Vibrio phage VP3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Chatterjeevirus; unclassified Chatterjeevirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9YAF0|H9YAF0_9CAUD Uncharacterized protein OS=Vibrio phage VP3 OX=588068 GN=VP3_002 PE=4 SV=1
MM1 pKa = 7.9SMQTNMMRR9 pKa = 11.84LEE11 pKa = 3.97AAMNRR16 pKa = 11.84LTMCFNISNMKK27 pKa = 8.84TVLGVYY33 pKa = 8.52QEE35 pKa = 4.56EE36 pKa = 4.6GQEE39 pKa = 3.81AATRR43 pKa = 11.84EE44 pKa = 4.17VSIKK48 pKa = 9.77MDD50 pKa = 3.78GLCWPEE56 pKa = 3.96VKK58 pKa = 10.47NLCTLFCEE66 pKa = 5.8DD67 pKa = 5.32FEE69 pKa = 4.43QDD71 pKa = 4.06CILVINNEE79 pKa = 4.01NGRR82 pKa = 11.84CALWSNSWSEE92 pKa = 4.26EE93 pKa = 3.88LGFWTQVTAEE103 pKa = 4.22EE104 pKa = 3.95AHH106 pKa = 6.27EE107 pKa = 4.27AGIYY111 pKa = 9.6TLDD114 pKa = 3.43TNFTYY119 pKa = 10.33WLAKK123 pKa = 10.01

Molecular weight: 14.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9YAF4|H9YAF4_9CAUD DNA-directed RNA polymerase OS=Vibrio phage VP3 OX=588068 GN=VP3_006 PE=3 SV=1
MM1 pKa = 7.1KK2 pKa = 9.48TSKK5 pKa = 10.36YY6 pKa = 10.21FKK8 pKa = 9.93AATMAMAYY16 pKa = 9.56GASTVIAYY24 pKa = 7.55NTTKK28 pKa = 10.68CFIDD32 pKa = 4.14CGLLNKK38 pKa = 9.97RR39 pKa = 11.84RR40 pKa = 11.84VKK42 pKa = 10.81KK43 pKa = 10.44FIKK46 pKa = 9.92KK47 pKa = 9.59HH48 pKa = 4.28NAKK51 pKa = 10.06IADD54 pKa = 3.69FRR56 pKa = 11.84GRR58 pKa = 11.84IYY60 pKa = 10.08PVRR63 pKa = 11.84YY64 pKa = 9.27IVV66 pKa = 3.37

Molecular weight: 7.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12179

38

1124

234.2

26.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.506 ± 0.475

1.1 ± 0.205

6.002 ± 0.234

7.127 ± 0.373

4.163 ± 0.2

6.848 ± 0.321

1.79 ± 0.208

5.641 ± 0.184

6.864 ± 0.328

8.309 ± 0.309

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.03 ± 0.2

4.623 ± 0.255

3.621 ± 0.158

4.27 ± 0.347

4.943 ± 0.157

6.019 ± 0.316

5.633 ± 0.301

6.388 ± 0.303

1.281 ± 0.188

3.843 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski