Avon-Heathcote Estuary associated circular virus 19

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IBR9|A0A0C5IBR9_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 19 OX=1618242 PE=3 SV=1
MM1 pKa = 7.03SRR3 pKa = 11.84KK4 pKa = 9.98RR5 pKa = 11.84NWCFTLFPDD14 pKa = 4.06QDD16 pKa = 3.48GYY18 pKa = 10.21YY19 pKa = 9.08TDD21 pKa = 5.42NIADD25 pKa = 4.2MVSGDD30 pKa = 3.91YY31 pKa = 11.03DD32 pKa = 3.75FEE34 pKa = 4.16EE35 pKa = 3.96QKK37 pKa = 10.37IRR39 pKa = 11.84FIIFQEE45 pKa = 4.62EE46 pKa = 4.2ICPEE50 pKa = 3.69SSRR53 pKa = 11.84RR54 pKa = 11.84HH55 pKa = 4.23LQGYY59 pKa = 7.96VEE61 pKa = 4.36YY62 pKa = 9.52TVSRR66 pKa = 11.84RR67 pKa = 11.84LAAVKK72 pKa = 10.33LSLGSEE78 pKa = 4.41SVHH81 pKa = 7.63LEE83 pKa = 3.6GRR85 pKa = 11.84RR86 pKa = 11.84GTASEE91 pKa = 3.7AAEE94 pKa = 4.12YY95 pKa = 10.55CEE97 pKa = 5.47KK98 pKa = 10.49EE99 pKa = 4.75DD100 pKa = 4.4SFAPDD105 pKa = 4.37GIRR108 pKa = 11.84FQWGEE113 pKa = 3.76LGGEE117 pKa = 3.79QGARR121 pKa = 11.84SDD123 pKa = 3.84LATVASAVISGKK135 pKa = 9.92RR136 pKa = 11.84VEE138 pKa = 4.4EE139 pKa = 4.13VALEE143 pKa = 4.14FPTTFIRR150 pKa = 11.84YY151 pKa = 8.54NRR153 pKa = 11.84GIEE156 pKa = 3.86KK157 pKa = 8.96LTNVVRR163 pKa = 11.84RR164 pKa = 11.84KK165 pKa = 9.05EE166 pKa = 3.79QMRR169 pKa = 11.84NLRR172 pKa = 11.84MLKK175 pKa = 10.26VVVLWGDD182 pKa = 3.03AGTGKK187 pKa = 8.25TRR189 pKa = 11.84TAFEE193 pKa = 5.05LSPDD197 pKa = 3.65HH198 pKa = 7.06YY199 pKa = 10.92ILSQQDD205 pKa = 3.29GQLWWDD211 pKa = 3.86GYY213 pKa = 10.88EE214 pKa = 4.32GQKK217 pKa = 10.61HH218 pKa = 6.5LIIDD222 pKa = 4.68DD223 pKa = 4.37FYY225 pKa = 11.9GWIKK229 pKa = 9.98WGLFLRR235 pKa = 11.84ILDD238 pKa = 4.5IYY240 pKa = 10.24PLQLPVKK247 pKa = 9.71GGFTIASWDD256 pKa = 3.59VVFITSNCMPRR267 pKa = 11.84NWYY270 pKa = 8.71EE271 pKa = 3.75RR272 pKa = 11.84GMPAEE277 pKa = 4.1LARR280 pKa = 11.84RR281 pKa = 11.84LTHH284 pKa = 6.26IVEE287 pKa = 4.37FEE289 pKa = 4.1SYY291 pKa = 8.62EE292 pKa = 3.99TDD294 pKa = 3.45FDD296 pKa = 4.5KK297 pKa = 11.02IKK299 pKa = 10.66EE300 pKa = 4.08ILVV303 pKa = 3.6

Molecular weight: 35.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IBR9|A0A0C5IBR9_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 19 OX=1618242 PE=3 SV=1
MM1 pKa = 7.08ARR3 pKa = 11.84SRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84YY8 pKa = 9.16KK9 pKa = 10.35KK10 pKa = 9.62SSRR13 pKa = 11.84SRR15 pKa = 11.84WSFPALHH22 pKa = 6.02YY23 pKa = 10.29PGYY26 pKa = 10.54NYY28 pKa = 10.52LGPGTKK34 pKa = 10.32NFDD37 pKa = 3.38RR38 pKa = 11.84APRR41 pKa = 11.84NDD43 pKa = 3.53LDD45 pKa = 3.69AAARR49 pKa = 11.84KK50 pKa = 9.45HH51 pKa = 6.2DD52 pKa = 4.19LDD54 pKa = 3.69YY55 pKa = 11.77GKK57 pKa = 10.12LQRR60 pKa = 11.84GGRR63 pKa = 11.84NPYY66 pKa = 9.92FKK68 pKa = 10.08WFKK71 pKa = 10.59ADD73 pKa = 3.38QRR75 pKa = 11.84FLDD78 pKa = 5.5RR79 pKa = 11.84IAKK82 pKa = 9.77DD83 pKa = 3.18KK84 pKa = 11.19SVPGWIARR92 pKa = 11.84QTFRR96 pKa = 11.84AKK98 pKa = 10.36KK99 pKa = 8.98YY100 pKa = 7.76FAPQGDD106 pKa = 4.25EE107 pKa = 4.33LPRR110 pKa = 11.84KK111 pKa = 8.29HH112 pKa = 5.69TGKK115 pKa = 10.24RR116 pKa = 11.84SRR118 pKa = 11.84SAIPVYY124 pKa = 10.68LIDD127 pKa = 4.14RR128 pKa = 11.84FSHH131 pKa = 6.55KK132 pKa = 9.96KK133 pKa = 9.74QKK135 pKa = 9.27TLGGSLKK142 pKa = 10.46KK143 pKa = 9.76MRR145 pKa = 11.84RR146 pKa = 11.84TWRR149 pKa = 11.84RR150 pKa = 11.84PRR152 pKa = 11.84ALKK155 pKa = 10.14RR156 pKa = 11.84RR157 pKa = 11.84RR158 pKa = 11.84SASYY162 pKa = 9.96GRR164 pKa = 11.84KK165 pKa = 8.91RR166 pKa = 11.84RR167 pKa = 11.84YY168 pKa = 9.07KK169 pKa = 9.14RR170 pKa = 11.84RR171 pKa = 11.84RR172 pKa = 11.84YY173 pKa = 9.83PSMKK177 pKa = 9.59PLYY180 pKa = 10.05RR181 pKa = 11.84KK182 pKa = 9.53IKK184 pKa = 10.15RR185 pKa = 11.84VAKK188 pKa = 10.02GLGRR192 pKa = 11.84MQHH195 pKa = 5.4QNAVANVYY203 pKa = 10.53RR204 pKa = 11.84DD205 pKa = 3.59VSSGNVSWDD214 pKa = 3.33AGEE217 pKa = 4.47CAYY220 pKa = 10.18KK221 pKa = 10.61DD222 pKa = 2.94ITAILTSGQMDD233 pKa = 3.51NVLSTFRR240 pKa = 11.84VLQMDD245 pKa = 3.82AGVNSRR251 pKa = 11.84EE252 pKa = 4.11TLDD255 pKa = 4.88LDD257 pKa = 4.07TGTATANVKK266 pKa = 9.55FVNARR271 pKa = 11.84SRR273 pKa = 11.84TVFRR277 pKa = 11.84NNTTQRR283 pKa = 11.84ANIAVYY289 pKa = 10.74VLTPTQDD296 pKa = 3.35LSNTPSSLVSSGLTTAEE313 pKa = 3.8ATAPSIDD320 pKa = 3.23IKK322 pKa = 10.64YY323 pKa = 9.27WPVDD327 pKa = 3.45SPIFRR332 pKa = 11.84RR333 pKa = 11.84YY334 pKa = 7.48WKK336 pKa = 9.81IVKK339 pKa = 9.83KK340 pKa = 9.2RR341 pKa = 11.84TRR343 pKa = 11.84ALDD346 pKa = 4.01PGDD349 pKa = 3.86EE350 pKa = 4.33MTVMMRR356 pKa = 11.84RR357 pKa = 11.84NKK359 pKa = 9.7PFSWNNKK366 pKa = 7.99PNDD369 pKa = 3.66VNAEE373 pKa = 4.13SYY375 pKa = 10.88RR376 pKa = 11.84GMKK379 pKa = 9.22SQVVMVRR386 pKa = 3.41

Molecular weight: 44.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

689

303

386

344.5

39.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.967 ± 0.861

0.726 ± 0.377

6.096 ± 0.111

4.79 ± 2.616

4.209 ± 0.68

6.676 ± 0.79

1.451 ± 0.126

4.644 ± 1.241

6.967 ± 1.489

6.967 ± 0.815

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.309

3.919 ± 1.021

4.209 ± 0.786

3.483 ± 0.303

10.885 ± 1.881

6.967 ± 0.651

5.515 ± 0.358

6.096 ± 0.111

2.467 ± 0.319

4.499 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski