Agrobacterium phage Atu_ph04

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; unclassified Ackermannviridae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223VZU8|A0A223VZU8_9CAUD Topoisomerase IV subunit A OS=Agrobacterium phage Atu_ph04 OX=2024263 PE=4 SV=2
MM1 pKa = 7.19VNPYY5 pKa = 9.87FKK7 pKa = 11.21SNFFDD12 pKa = 4.18NEE14 pKa = 4.03MNLLADD20 pKa = 4.15LTDD23 pKa = 3.42EE24 pKa = 5.44AIYY27 pKa = 8.11TTGIEE32 pKa = 4.22VEE34 pKa = 4.18YY35 pKa = 10.33LPRR38 pKa = 11.84KK39 pKa = 9.1IVQHH43 pKa = 6.17DD44 pKa = 4.18PIMNEE49 pKa = 3.82PLSSQFNEE57 pKa = 4.63VYY59 pKa = 10.35QIDD62 pKa = 3.86MAIEE66 pKa = 4.04STEE69 pKa = 4.04SFFAGNEE76 pKa = 4.32SISSIGLAFSFASSSLSVSRR96 pKa = 11.84RR97 pKa = 11.84KK98 pKa = 8.47FTSVTGMQEE107 pKa = 3.98PLNGDD112 pKa = 4.42LIFIKK117 pKa = 9.97QNQMLFEE124 pKa = 4.18IVNTNVRR131 pKa = 11.84DD132 pKa = 3.72PLISGGRR139 pKa = 11.84HH140 pKa = 3.34FTFTIFVKK148 pKa = 10.22PFSIGEE154 pKa = 4.26GNSSFKK160 pKa = 10.39EE161 pKa = 4.25SKK163 pKa = 10.6FKK165 pKa = 10.85GSRR168 pKa = 11.84DD169 pKa = 3.57LQEE172 pKa = 4.12TLSNFLGVVDD182 pKa = 3.84QKK184 pKa = 11.47EE185 pKa = 4.11WDD187 pKa = 3.85FLDD190 pKa = 4.16ASVDD194 pKa = 4.2SVTADD199 pKa = 3.41NNVLRR204 pKa = 11.84VDD206 pKa = 3.81VEE208 pKa = 4.26EE209 pKa = 4.99SGLSQIVRR217 pKa = 11.84ADD219 pKa = 3.65SEE221 pKa = 4.88TFTADD226 pKa = 4.05CEE228 pKa = 4.35LTADD232 pKa = 4.62DD233 pKa = 6.62LLGVWNNSIDD243 pKa = 3.91DD244 pKa = 3.74VMRR247 pKa = 11.84TMDD250 pKa = 3.32QKK252 pKa = 11.09IPNFSDD258 pKa = 2.95NGEE261 pKa = 4.19FEE263 pKa = 4.41CVEE266 pKa = 4.08EE267 pKa = 5.34KK268 pKa = 10.82ILVDD272 pKa = 3.15KK273 pKa = 9.67TNPFGFFF280 pKa = 3.32

Molecular weight: 31.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223W0H2|A0A223W0H2_9CAUD Single stranded DNA-binding protein OS=Agrobacterium phage Atu_ph04 OX=2024263 PE=4 SV=1
MM1 pKa = 8.16AEE3 pKa = 4.07EE4 pKa = 4.9DD5 pKa = 3.84KK6 pKa = 11.23DD7 pKa = 3.88VQKK10 pKa = 9.93TFDD13 pKa = 4.43EE14 pKa = 4.45IVKK17 pKa = 10.21SLEE20 pKa = 3.91RR21 pKa = 11.84GMSSSEE27 pKa = 3.37IAKK30 pKa = 9.81RR31 pKa = 11.84QRR33 pKa = 11.84EE34 pKa = 4.34SIAYY38 pKa = 9.34FSGRR42 pKa = 11.84NNAKK46 pKa = 8.79TVDD49 pKa = 3.33TGRR52 pKa = 11.84ANLSMMTKK60 pKa = 9.48GQRR63 pKa = 11.84GVSDD67 pKa = 5.52LIPGTMMTYY76 pKa = 10.38AYY78 pKa = 9.73SAKK81 pKa = 10.27NAEE84 pKa = 4.32TLPYY88 pKa = 9.1WDD90 pKa = 4.86RR91 pKa = 11.84FPLIVFLDD99 pKa = 3.74VQKK102 pKa = 11.26NGNLLALNMHH112 pKa = 6.67YY113 pKa = 10.52LPPDD117 pKa = 3.82FRR119 pKa = 11.84AKK121 pKa = 10.14ILAEE125 pKa = 3.91LMKK128 pKa = 10.4TVTAKK133 pKa = 10.38EE134 pKa = 3.91LRR136 pKa = 11.84HH137 pKa = 5.69DD138 pKa = 3.22VRR140 pKa = 11.84MRR142 pKa = 11.84ITYY145 pKa = 7.48EE146 pKa = 3.43KK147 pKa = 9.14CQRR150 pKa = 11.84IAAFQPLQFALKK162 pKa = 10.1SYY164 pKa = 9.57IPQRR168 pKa = 11.84ISTKK172 pKa = 9.53IVRR175 pKa = 11.84IQPDD179 pKa = 3.27AWHH182 pKa = 6.55HH183 pKa = 6.5AIFFPSQMFVNASEE197 pKa = 4.24RR198 pKa = 11.84KK199 pKa = 7.8VWSDD203 pKa = 3.36FRR205 pKa = 11.84RR206 pKa = 11.84FRR208 pKa = 4.68

Molecular weight: 24.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

19150

105

1695

399.0

44.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.313 ± 0.253

0.768 ± 0.078

6.695 ± 0.166

7.368 ± 0.423

5.097 ± 0.201

6.559 ± 0.459

1.749 ± 0.173

6.71 ± 0.23

6.971 ± 0.359

7.54 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.136 ± 0.128

5.441 ± 0.209

3.603 ± 0.179

2.94 ± 0.124

5.196 ± 0.188

7.661 ± 0.379

5.676 ± 0.342

7.097 ± 0.29

1.05 ± 0.083

3.431 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski