Streptococcus satellite phage Javan621

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZX80|A0A4D5ZX80_9VIRU Transcriptional regulator OS=Streptococcus satellite phage Javan621 OX=2558789 GN=JavanS621_0013 PE=4 SV=1
MM1 pKa = 7.46MKK3 pKa = 9.51TLSDD7 pKa = 3.62TPNTYY12 pKa = 9.07TFTATLPNFEE22 pKa = 4.12QAQRR26 pKa = 11.84AGSALIGYY34 pKa = 7.54MIGTYY39 pKa = 7.29EE40 pKa = 4.18QSVIDD45 pKa = 3.34ITYY48 pKa = 8.82TGNGDD53 pKa = 3.39IEE55 pKa = 4.38VEE57 pKa = 4.29YY58 pKa = 10.52TSDD61 pKa = 3.79EE62 pKa = 4.26DD63 pKa = 3.41LTEE66 pKa = 4.01NFEE69 pKa = 5.15RR70 pKa = 11.84ITNSLDD76 pKa = 3.23HH77 pKa = 6.91TDD79 pKa = 4.07DD80 pKa = 4.3FEE82 pKa = 7.25DD83 pKa = 4.38YY84 pKa = 11.27EE85 pKa = 4.49EE86 pKa = 5.88DD87 pKa = 3.84EE88 pKa = 4.46PTLKK92 pKa = 10.84GDD94 pKa = 3.97LDD96 pKa = 4.48AYY98 pKa = 8.91TALIGSFEE106 pKa = 4.4TLGQAQAFTEE116 pKa = 4.08NLDD119 pKa = 4.44DD120 pKa = 6.72DD121 pKa = 4.55LTNGNNFVFEE131 pKa = 4.14QRR133 pKa = 11.84PDD135 pKa = 3.29AVVVYY140 pKa = 8.41VSPQDD145 pKa = 3.62TIANSTIQKK154 pKa = 10.38LEE156 pKa = 3.57EE157 pKa = 4.35AYY159 pKa = 11.02VKK161 pKa = 10.6FEE163 pKa = 4.68ADD165 pKa = 3.57YY166 pKa = 11.21EE167 pKa = 4.39PDD169 pKa = 3.79DD170 pKa = 4.4FRR172 pKa = 11.84LNYY175 pKa = 10.47LDD177 pKa = 4.46MRR179 pKa = 11.84VQQLQSLDD187 pKa = 3.73KK188 pKa = 10.18DD189 pKa = 3.95TLISDD194 pKa = 4.33IINYY198 pKa = 8.55EE199 pKa = 4.29LEE201 pKa = 4.19LLDD204 pKa = 3.85YY205 pKa = 11.08ADD207 pKa = 5.15RR208 pKa = 11.84LLSDD212 pKa = 4.1EE213 pKa = 4.68PLPLNSLDD221 pKa = 4.93GYY223 pKa = 10.62DD224 pKa = 3.61TMEE227 pKa = 4.41LLGDD231 pKa = 3.85EE232 pKa = 5.11VIDD235 pKa = 4.73LVKK238 pKa = 10.14TLDD241 pKa = 3.35TDD243 pKa = 3.61KK244 pKa = 11.16QFDD247 pKa = 4.55GIHH250 pKa = 7.15DD251 pKa = 4.28YY252 pKa = 10.98IIGGG256 pKa = 3.65

Molecular weight: 29.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZT23|A0A4D5ZT23_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan621 OX=2558789 GN=JavanS621_0001 PE=4 SV=1
MM1 pKa = 7.83KK2 pKa = 10.07IIEE5 pKa = 4.27YY6 pKa = 9.79KK7 pKa = 10.74KK8 pKa = 10.11KK9 pKa = 10.53DD10 pKa = 3.31GTIMYY15 pKa = 9.41RR16 pKa = 11.84SQVYY20 pKa = 10.43LGVDD24 pKa = 3.95RR25 pKa = 11.84ITGKK29 pKa = 10.47KK30 pKa = 9.94SRR32 pKa = 11.84TSVSGRR38 pKa = 11.84TKK40 pKa = 10.78KK41 pKa = 9.89EE42 pKa = 3.5LKK44 pKa = 10.87NNIKK48 pKa = 9.97LAKK51 pKa = 10.26FEE53 pKa = 4.23FQANGEE59 pKa = 4.3TLSKK63 pKa = 10.25KK64 pKa = 9.62VQVKK68 pKa = 7.02TFKK71 pKa = 10.88DD72 pKa = 3.81LTDD75 pKa = 3.2MWLVNYY81 pKa = 10.29KK82 pKa = 8.93LTLKK86 pKa = 9.06PQSYY90 pKa = 10.34NSTVSMLNKK99 pKa = 10.17HH100 pKa = 5.7ILPYY104 pKa = 9.19FGKK107 pKa = 9.79MKK109 pKa = 10.19LDD111 pKa = 4.68KK112 pKa = 9.82ITSSDD117 pKa = 3.25VQTFINSLSKK127 pKa = 10.05KK128 pKa = 8.11TINYY132 pKa = 8.86VNARR136 pKa = 11.84SIISRR141 pKa = 11.84ILQHH145 pKa = 6.69GVLLQLIQHH154 pKa = 6.08NPARR158 pKa = 11.84DD159 pKa = 3.15IVLPRR164 pKa = 11.84KK165 pKa = 8.88QKK167 pKa = 10.58KK168 pKa = 10.47DD169 pKa = 2.97SDD171 pKa = 3.74KK172 pKa = 11.55VKK174 pKa = 10.67FIEE177 pKa = 5.41KK178 pKa = 10.28DD179 pKa = 3.47DD180 pKa = 4.03LKK182 pKa = 11.36KK183 pKa = 10.77FLDD186 pKa = 4.19YY187 pKa = 10.73IEE189 pKa = 4.82KK190 pKa = 10.73YY191 pKa = 10.29KK192 pKa = 10.83LRR194 pKa = 11.84NYY196 pKa = 7.56KK197 pKa = 9.94TNFNYY202 pKa = 9.99VLYY205 pKa = 10.41KK206 pKa = 10.85LLLATGLRR214 pKa = 11.84IGEE217 pKa = 4.17ACALEE222 pKa = 4.32WSDD225 pKa = 3.79IDD227 pKa = 5.93LINGTINIDD236 pKa = 3.1KK237 pKa = 9.64TYY239 pKa = 10.23SKK241 pKa = 10.54SIRR244 pKa = 11.84RR245 pKa = 11.84LSTTKK250 pKa = 10.45SKK252 pKa = 10.98SGTRR256 pKa = 11.84LISIDD261 pKa = 3.42QATVNMLKK269 pKa = 10.42LYY271 pKa = 8.44QVRR274 pKa = 11.84QRR276 pKa = 11.84QLFHH280 pKa = 6.73EE281 pKa = 4.87VGGKK285 pKa = 9.36APKK288 pKa = 10.35VVFATPTRR296 pKa = 11.84KK297 pKa = 9.63YY298 pKa = 8.28FHH300 pKa = 6.38TFVRR304 pKa = 11.84QNALDD309 pKa = 3.89RR310 pKa = 11.84ACIEE314 pKa = 3.8AGIPRR319 pKa = 11.84FTFHH323 pKa = 7.74AFRR326 pKa = 11.84HH327 pKa = 4.69THH329 pKa = 6.92ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.52RR346 pKa = 11.84LGHH349 pKa = 5.94SNIAMTLDD357 pKa = 3.93VYY359 pKa = 11.5SHH361 pKa = 7.06LSKK364 pKa = 11.04DD365 pKa = 3.51KK366 pKa = 10.41EE367 pKa = 4.26KK368 pKa = 10.88EE369 pKa = 3.87AVSYY373 pKa = 9.94FEE375 pKa = 4.94KK376 pKa = 10.6AINSII381 pKa = 3.84

Molecular weight: 44.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2301

47

479

153.4

17.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.259 ± 0.35

0.565 ± 0.146

6.997 ± 0.896

7.127 ± 0.852

5.085 ± 0.611

4.433 ± 0.311

1.304 ± 0.296

8.17 ± 0.505

8.953 ± 1.098

9.735 ± 0.666

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.694 ± 0.346

6.736 ± 0.487

3.477 ± 0.557

3.651 ± 0.538

4.65 ± 0.377

5.476 ± 0.616

6.215 ± 0.786

4.694 ± 0.404

0.869 ± 0.267

4.911 ± 0.433
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski