Streptomyces sp. (strain SirexAA-E / ActE)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6354 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2NJP1|G2NJP1_STREK NCS1 nucleoside transporter family OS=Streptomyces sp. (strain SirexAA-E / ActE) OX=862751 GN=SACTE_5615 PE=3 SV=1
MM1 pKa = 7.57SYY3 pKa = 11.34DD4 pKa = 3.35LAVWDD9 pKa = 4.89GEE11 pKa = 4.59RR12 pKa = 11.84PLNDD16 pKa = 3.88DD17 pKa = 3.66AAGSAFDD24 pKa = 3.95EE25 pKa = 4.55MYY27 pKa = 10.71EE28 pKa = 4.19RR29 pKa = 11.84YY30 pKa = 10.13LEE32 pKa = 4.42SEE34 pKa = 4.54DD35 pKa = 3.63VAVPPFPRR43 pKa = 11.84IKK45 pKa = 10.45AYY47 pKa = 10.85VNALIEE53 pKa = 4.74RR54 pKa = 11.84YY55 pKa = 9.73PEE57 pKa = 4.49DD58 pKa = 5.36DD59 pKa = 4.88RR60 pKa = 11.84DD61 pKa = 4.35SPWASPPVIDD71 pKa = 4.61EE72 pKa = 4.08ASGPIVYY79 pKa = 10.74LLMSYY84 pKa = 10.53SRR86 pKa = 11.84AEE88 pKa = 4.1EE89 pKa = 3.93VSEE92 pKa = 4.2YY93 pKa = 10.71AASLARR99 pKa = 11.84EE100 pKa = 4.05YY101 pKa = 11.12EE102 pKa = 4.51LICFDD107 pKa = 4.08PQGEE111 pKa = 4.45TLRR114 pKa = 11.84SS115 pKa = 3.4

Molecular weight: 13.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2NF06|G2NF06_STREK Alpha/beta hydrolase fold protein OS=Streptomyces sp. (strain SirexAA-E / ActE) OX=862751 GN=SACTE_3620 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ANLSAA45 pKa = 4.66

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6354

0

6354

2142609

32

13652

337.2

36.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.661 ± 0.051

0.752 ± 0.009

6.075 ± 0.025

5.728 ± 0.027

2.708 ± 0.017

9.675 ± 0.031

2.257 ± 0.016

2.991 ± 0.024

2.085 ± 0.028

10.286 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.735 ± 0.014

1.677 ± 0.015

6.171 ± 0.028

2.678 ± 0.019

8.098 ± 0.037

5.103 ± 0.024

6.212 ± 0.029

8.594 ± 0.031

1.47 ± 0.012

2.043 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski