Halobacillus halophilus (strain ATCC 35676 / DSM 2266 / JCM 20832 / KCTC 3685 / LMG 17431 / NBRC 102448 / NCIMB 2269) (Sporosarcina halophila)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Halobacillus; Halobacillus halophilus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0JI36|I0JI36_HALH3 Exopolysaccharide biosynthesis protein OS=Halobacillus halophilus (strain ATCC 35676 / DSM 2266 / JCM 20832 / KCTC 3685 / LMG 17431 / NBRC 102448 / NCIMB 2269) OX=866895 GN=HBHAL_1433 PE=3 SV=1
MM1 pKa = 7.21EE2 pKa = 6.15WIRR5 pKa = 11.84LNPDD9 pKa = 2.53ICSLFSNDD17 pKa = 3.89SVLVCYY23 pKa = 9.26LTDD26 pKa = 3.55EE27 pKa = 4.73LGEE30 pKa = 4.25TVEE33 pKa = 5.66SITCRR38 pKa = 11.84EE39 pKa = 3.99SGSRR43 pKa = 11.84EE44 pKa = 4.05DD45 pKa = 3.42VTVTLSGEE53 pKa = 4.38SVVLQRR59 pKa = 11.84VSLINQGFVVVEE71 pKa = 4.08ISDD74 pKa = 5.25DD75 pKa = 3.67EE76 pKa = 4.76TTCITDD82 pKa = 4.69PIPFCFVEE90 pKa = 4.28TLLLCAPDD98 pKa = 3.9GTDD101 pKa = 3.24IEE103 pKa = 4.89CKK105 pKa = 9.26VTEE108 pKa = 4.9FDD110 pKa = 4.24CQACVNCQNGEE121 pKa = 4.11FLSLDD126 pKa = 3.24IFFDD130 pKa = 3.63VCLSVQTVVDD140 pKa = 4.09TVFEE144 pKa = 4.36LPISFCVPRR153 pKa = 11.84KK154 pKa = 9.81EE155 pKa = 4.83IEE157 pKa = 4.07QPPCTFKK164 pKa = 10.84IPNQCPTTFSEE175 pKa = 5.36DD176 pKa = 3.19STEE179 pKa = 4.12RR180 pKa = 11.84KK181 pKa = 8.9QVQPEE186 pKa = 3.99VNEE189 pKa = 4.04ILNLPAPQPTPSQQQEE205 pKa = 4.57VACVSATKK213 pKa = 10.52VYY215 pKa = 10.87DD216 pKa = 3.31WVVSQNSFRR225 pKa = 11.84ITRR228 pKa = 11.84NQADD232 pKa = 3.37ITFQCFPCEE241 pKa = 3.54VDD243 pKa = 4.02LFVPADD249 pKa = 3.71IFCTNVISGRR259 pKa = 11.84VVCATIPIEE268 pKa = 4.22GAEE271 pKa = 4.33VTLSGSPDD279 pKa = 3.16LVLYY283 pKa = 10.8DD284 pKa = 4.24MNPVTTDD291 pKa = 2.82EE292 pKa = 4.24NGYY295 pKa = 10.38FDD297 pKa = 4.62VNVEE301 pKa = 3.99IPEE304 pKa = 4.27DD305 pKa = 3.94TEE307 pKa = 4.19ATNVTIMAEE316 pKa = 4.07AIVRR320 pKa = 11.84SVVASASTEE329 pKa = 4.25TVVEE333 pKa = 4.5CPEE336 pKa = 4.52DD337 pKa = 3.42PCGIDD342 pKa = 3.77LFAPATITCDD352 pKa = 3.18DD353 pKa = 3.92FMDD356 pKa = 4.32GRR358 pKa = 11.84VRR360 pKa = 11.84CNGRR364 pKa = 11.84LIEE367 pKa = 4.42GALVTFDD374 pKa = 4.01SNAPGIVTFEE384 pKa = 4.14PPQIEE389 pKa = 4.29TDD391 pKa = 3.52SLGDD395 pKa = 3.37YY396 pKa = 7.51FTGVRR401 pKa = 11.84ITNGTAPQTVTLIASTTVEE420 pKa = 3.99GQMVSDD426 pKa = 3.98SVDD429 pKa = 3.25VTVNCPFDD437 pKa = 4.16ACILTINVPQLIDD450 pKa = 3.63CMAVITGNISCNGMGIEE467 pKa = 3.96GAEE470 pKa = 3.97IFFSDD475 pKa = 4.12FPEE478 pKa = 4.32DD479 pKa = 3.58VVTYY483 pKa = 9.12EE484 pKa = 4.31PNPAISDD491 pKa = 3.57INGDD495 pKa = 3.64FTTTIMVPEE504 pKa = 4.66GTSLTDD510 pKa = 3.14IDD512 pKa = 4.62VEE514 pKa = 4.3ATTTVQGQMVEE525 pKa = 4.56ANFGTQIICLPEE537 pKa = 3.8EE538 pKa = 4.74CPCKK542 pKa = 10.39FRR544 pKa = 11.84IRR546 pKa = 11.84PRR548 pKa = 11.84GSRR551 pKa = 11.84TRR553 pKa = 11.84ATVEE557 pKa = 3.64LTQNGMVTFLDD568 pKa = 5.02GIINLSAIQCFRR580 pKa = 11.84IVPGCNPDD588 pKa = 3.12VDD590 pKa = 4.23DD591 pKa = 4.69FSVVFRR597 pKa = 11.84TDD599 pKa = 2.98QEE601 pKa = 4.29VIRR604 pKa = 11.84FVQGRR609 pKa = 11.84RR610 pKa = 11.84LDD612 pKa = 3.87IEE614 pKa = 4.59CEE616 pKa = 4.23SNTFARR622 pKa = 11.84VHH624 pKa = 5.38GTATAEE630 pKa = 4.12GNVLNGLFDD639 pKa = 3.65VTIEE643 pKa = 4.2LSITAPDD650 pKa = 3.15IGVWTVSATDD660 pKa = 3.29NMGNTFFTSFVQRR673 pKa = 11.84VSRR676 pKa = 11.84STFIGDD682 pKa = 4.78CDD684 pKa = 4.03DD685 pKa = 4.41APNN688 pKa = 3.56

Molecular weight: 75.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0JH13|I0JH13_HALH3 DNA polymerase III delta' subunit OS=Halobacillus halophilus (strain ATCC 35676 / DSM 2266 / JCM 20832 / KCTC 3685 / LMG 17431 / NBRC 102448 / NCIMB 2269) OX=866895 GN=holB PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.46VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MSSPNGRR28 pKa = 11.84KK29 pKa = 9.21VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4100

0

4100

1128406

30

1526

275.2

30.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.866 ± 0.042

0.623 ± 0.011

5.307 ± 0.036

8.053 ± 0.054

4.494 ± 0.034

6.981 ± 0.046

2.285 ± 0.021

7.153 ± 0.04

6.329 ± 0.039

9.56 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.919 ± 0.018

4.182 ± 0.027

3.745 ± 0.021

3.995 ± 0.028

4.244 ± 0.031

6.309 ± 0.03

5.318 ± 0.028

6.95 ± 0.033

1.1 ± 0.017

3.588 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski