Fusobacterium sp. CAG:439
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2311 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7JE10|R7JE10_9FUSO Transcriptional regulator LuxR family OS=Fusobacterium sp. CAG:439 OX=1262899 GN=BN657_00084 PE=4 SV=1
MM1 pKa = 7.66 QYY3 pKa = 10.99 QNDD6 pKa = 3.82 RR7 pKa = 11.84 NAVNLVEE14 pKa = 5.57 AGLNSQDD21 pKa = 3.21 AANNFIEE28 pKa = 4.91 TYY30 pKa = 9.63 FKK32 pKa = 11.11 VVNKK36 pKa = 10.04 CDD38 pKa = 3.7 NLSGCFADD46 pKa = 3.84 SYY48 pKa = 11.32 KK49 pKa = 10.71 KK50 pKa = 10.58 ISGADD55 pKa = 3.35 AGIGDD60 pKa = 3.88 YY61 pKa = 10.68 SSYY64 pKa = 11.2 VIASGAALRR73 pKa = 11.84 PAYY76 pKa = 10.53 NRR78 pKa = 11.84 VDD80 pKa = 3.52 DD81 pKa = 4.23 SVISFLVDD89 pKa = 3.19 VNGKK93 pKa = 9.32 QGPNIAGRR101 pKa = 11.84 DD102 pKa = 3.77 LFIMCLYY109 pKa = 10.73 NNGVLDD115 pKa = 4.14 DD116 pKa = 4.36 ANYY119 pKa = 11.02 NIDD122 pKa = 4.59 DD123 pKa = 5.21 DD124 pKa = 5.14 GNTVNFSGAPSEE136 pKa = 4.34 EE137 pKa = 3.81 EE138 pKa = 4.1 RR139 pKa = 11.84 NDD141 pKa = 3.8 LFNSQCISSNSDD153 pKa = 3.45 GISGCFGKK161 pKa = 10.31 ILNDD165 pKa = 3.21 NWEE168 pKa = 3.95 MTYY171 pKa = 11.36
Molecular weight: 18.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.935
IPC_protein 3.935
Toseland 3.694
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.897
Rodwell 3.757
Grimsley 3.605
Solomon 3.923
Lehninger 3.884
Nozaki 4.05
DTASelect 4.329
Thurlkill 3.77
EMBOSS 3.897
Sillero 4.05
Patrickios 0.782
IPC_peptide 3.923
IPC2_peptide 4.024
IPC2.peptide.svr19 3.932
Protein with the highest isoelectric point:
>tr|R7JEA2|R7JEA2_9FUSO Anhydro-N-acetylmuramic acid kinase OS=Fusobacterium sp. CAG:439 OX=1262899 GN=anmK PE=3 SV=1
MM1 pKa = 7.42 ARR3 pKa = 11.84 LAGVDD8 pKa = 3.49 LPRR11 pKa = 11.84 NKK13 pKa = 10.15 RR14 pKa = 11.84 MEE16 pKa = 3.88 IALTYY21 pKa = 9.91 IYY23 pKa = 10.66 GIGPTRR29 pKa = 11.84 AKK31 pKa = 10.41 KK32 pKa = 10.38 ILDD35 pKa = 3.53 ATKK38 pKa = 10.09 ISPDD42 pKa = 3.68 LRR44 pKa = 11.84 TDD46 pKa = 3.54 DD47 pKa = 5.08 LTDD50 pKa = 3.53 EE51 pKa = 5.08 DD52 pKa = 4.55 IKK54 pKa = 11.3 LLRR57 pKa = 11.84 NEE59 pKa = 4.02 LANYY63 pKa = 9.4 HH64 pKa = 6.41 IEE66 pKa = 4.36 GDD68 pKa = 3.51 LRR70 pKa = 11.84 RR71 pKa = 11.84 EE72 pKa = 4.02 VTLHH76 pKa = 6.11 IKK78 pKa = 10.22 RR79 pKa = 11.84 LQEE82 pKa = 3.7 IGSYY86 pKa = 9.98 RR87 pKa = 11.84 GLRR90 pKa = 11.84 HH91 pKa = 6.3 KK92 pKa = 10.86 RR93 pKa = 11.84 NLPCRR98 pKa = 11.84 GQRR101 pKa = 11.84 TKK103 pKa = 10.84 TNARR107 pKa = 11.84 TRR109 pKa = 11.84 RR110 pKa = 11.84 GKK112 pKa = 10.45 KK113 pKa = 9.91 GLAITKK119 pKa = 10.11 KK120 pKa = 9.23 KK121 pKa = 8.2 TAA123 pKa = 3.9
Molecular weight: 14.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.736
IPC_protein 10.394
Toseland 10.833
ProMoST 10.452
Dawson 10.906
Bjellqvist 10.57
Wikipedia 11.082
Rodwell 11.213
Grimsley 10.935
Solomon 11.008
Lehninger 10.979
Nozaki 10.804
DTASelect 10.57
Thurlkill 10.804
EMBOSS 11.228
Sillero 10.833
Patrickios 10.935
IPC_peptide 11.023
IPC2_peptide 9.311
IPC2.peptide.svr19 8.698
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2311
0
2311
640807
30
1484
277.3
31.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.872 ± 0.057
1.345 ± 0.024
5.827 ± 0.045
6.885 ± 0.056
4.534 ± 0.038
6.001 ± 0.056
1.515 ± 0.019
8.164 ± 0.049
8.974 ± 0.053
8.887 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.5 ± 0.027
6.328 ± 0.061
3.277 ± 0.028
3.237 ± 0.028
3.488 ± 0.029
5.942 ± 0.036
5.343 ± 0.038
6.018 ± 0.048
0.724 ± 0.017
4.134 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here