Amino acid dipepetide frequency for Fusobacterium sp. CAG:439

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.289AlaAla: 5.289 ± 0.131	1.008AlaCys: 1.008 ± 0.046	4.014AlaAsp: 4.014 ± 0.086	4.864AlaGlu: 4.864 ± 0.1	2.892AlaPhe: 2.892 ± 0.067	4.852AlaGly: 4.852 ± 0.099	1.021AlaHis: 1.021 ± 0.039	5.076AlaIle: 5.076 ± 0.109	6.105AlaLys: 6.105 ± 0.117	6.684AlaLeu: 6.684 ± 0.121	1.877AlaMet: 1.877 ± 0.061	3.411AlaAsn: 3.411 ± 0.078	1.952AlaPro: 1.952 ± 0.061	2.653AlaGln: 2.653 ± 0.074	2.492AlaArg: 2.492 ± 0.073	3.997AlaSer: 3.997 ± 0.088	2.619AlaThr: 2.619 ± 0.082	4.995AlaVal: 4.995 ± 0.09	0.389AlaTrp: 0.389 ± 0.025	2.534AlaTyr: 2.534 ± 0.07	0.0AlaXaa: 0.0 ± 0.0
Cys	0.985CysAla: 0.985 ± 0.038	0.245CysCys: 0.245 ± 0.021	0.874CysAsp: 0.874 ± 0.043	0.946CysGlu: 0.946 ± 0.041	0.715CysPhe: 0.715 ± 0.033	1.23CysGly: 1.23 ± 0.05	0.223CysHis: 0.223 ± 0.017	0.955CysIle: 0.955 ± 0.045	1.013CysLys: 1.013 ± 0.041	0.949CysLeu: 0.949 ± 0.039	0.304CysMet: 0.304 ± 0.024	0.634CysAsn: 0.634 ± 0.035	0.637CysPro: 0.637 ± 0.04	0.343CysGln: 0.343 ± 0.026	0.456CysArg: 0.456 ± 0.029	0.869CysSer: 0.869 ± 0.038	0.663CysThr: 0.663 ± 0.034	0.808CysVal: 0.808 ± 0.04	0.112CysTrp: 0.112 ± 0.017	0.492CysTyr: 0.492 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	3.805AspAla: 3.805 ± 0.076	0.877AspCys: 0.877 ± 0.042	3.363AspAsp: 3.363 ± 0.085	4.6AspGlu: 4.6 ± 0.107	3.251AspPhe: 3.251 ± 0.075	3.767AspGly: 3.767 ± 0.091	0.474AspHis: 0.474 ± 0.026	5.537AspIle: 5.537 ± 0.107	5.679AspLys: 5.679 ± 0.089	5.115AspLeu: 5.115 ± 0.11	1.483AspMet: 1.483 ± 0.044	3.589AspAsn: 3.589 ± 0.088	1.383AspPro: 1.383 ± 0.052	0.85AspGln: 0.85 ± 0.039	1.874AspArg: 1.874 ± 0.06	2.228AspSer: 2.228 ± 0.056	2.979AspThr: 2.979 ± 0.127	3.728AspVal: 3.728 ± 0.075	0.548AspTrp: 0.548 ± 0.03	3.135AspTyr: 3.135 ± 0.068	0.0AspXaa: 0.0 ± 0.0
Glu	4.515GluAla: 4.515 ± 0.111	0.797GluCys: 0.797 ± 0.037	3.603GluAsp: 3.603 ± 0.083	5.044GluGlu: 5.044 ± 0.102	3.262GluPhe: 3.262 ± 0.076	3.146GluGly: 3.146 ± 0.077	1.144GluHis: 1.144 ± 0.043	6.233GluIle: 6.233 ± 0.116	7.358GluLys: 7.358 ± 0.127	6.643GluLeu: 6.643 ± 0.11	1.832GluMet: 1.832 ± 0.06	5.29GluAsn: 5.29 ± 0.093	1.559GluPro: 1.559 ± 0.057	2.509GluGln: 2.509 ± 0.069	2.569GluArg: 2.569 ± 0.071	2.369GluSer: 2.369 ± 0.06	3.572GluThr: 3.572 ± 0.073	3.89GluVal: 3.89 ± 0.08	0.481GluTrp: 0.481 ± 0.03	3.032GluTyr: 3.032 ± 0.077	0.0GluXaa: 0.0 ± 0.0
Phe	3.265PheAla: 3.265 ± 0.074	0.613PheCys: 0.613 ± 0.031	3.11PheAsp: 3.11 ± 0.084	3.252PheGlu: 3.252 ± 0.074	1.893PhePhe: 1.893 ± 0.064	2.825PheGly: 2.825 ± 0.067	0.654PheHis: 0.654 ± 0.029	3.619PheIle: 3.619 ± 0.098	3.858PheLys: 3.858 ± 0.083	3.766PheLeu: 3.766 ± 0.089	1.153PheMet: 1.153 ± 0.043	2.786PheAsn: 2.786 ± 0.08	1.255PhePro: 1.255 ± 0.045	1.209PheGln: 1.209 ± 0.044	1.404PheArg: 1.404 ± 0.049	3.093PheSer: 3.093 ± 0.076	2.481PheThr: 2.481 ± 0.062	2.837PheVal: 2.837 ± 0.071	0.365PheTrp: 0.365 ± 0.024	1.902PheTyr: 1.902 ± 0.059	0.0PheXaa: 0.0 ± 0.0
Gly	4.224GlyAla: 4.224 ± 0.111	1.108GlyCys: 1.108 ± 0.045	3.045GlyAsp: 3.045 ± 0.068	3.605GlyGlu: 3.605 ± 0.079	2.979GlyPhe: 2.979 ± 0.065	4.057GlyGly: 4.057 ± 0.132	1.063GlyHis: 1.063 ± 0.041	5.339GlyIle: 5.339 ± 0.101	5.262GlyLys: 5.262 ± 0.094	5.278GlyLeu: 5.278 ± 0.103	1.631GlyMet: 1.631 ± 0.054	3.066GlyAsn: 3.066 ± 0.09	1.138GlyPro: 1.138 ± 0.047	1.785GlyGln: 1.785 ± 0.056	2.294GlyArg: 2.294 ± 0.069	3.497GlySer: 3.497 ± 0.096	3.519GlyThr: 3.519 ± 0.093	3.987GlyVal: 3.987 ± 0.086	0.568GlyTrp: 0.568 ± 0.034	2.564GlyTyr: 2.564 ± 0.064	0.005GlyXaa: 0.005 ± 0.003
His	0.904HisAla: 0.904 ± 0.037	0.247HisCys: 0.247 ± 0.02	0.785HisAsp: 0.785 ± 0.038	0.938HisGlu: 0.938 ± 0.044	0.719HisPhe: 0.719 ± 0.029	0.922HisGly: 0.922 ± 0.04	0.303HisHis: 0.303 ± 0.024	1.256HisIle: 1.256 ± 0.043	1.358HisLys: 1.358 ± 0.05	1.289HisLeu: 1.289 ± 0.041	0.301HisMet: 0.301 ± 0.02	0.994HisAsn: 0.994 ± 0.042	0.802HisPro: 0.802 ± 0.034	0.487HisGln: 0.487 ± 0.028	0.535HisArg: 0.535 ± 0.03	0.935HisSer: 0.935 ± 0.04	0.819HisThr: 0.819 ± 0.038	0.755HisVal: 0.755 ± 0.034	0.148HisTrp: 0.148 ± 0.019	0.655HisTyr: 0.655 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.96IleAla: 5.96 ± 0.106	1.066IleCys: 1.066 ± 0.046	5.023IleAsp: 5.023 ± 0.093	5.733IleGlu: 5.733 ± 0.094	3.503IlePhe: 3.503 ± 0.094	4.771IleGly: 4.771 ± 0.099	1.069IleHis: 1.069 ± 0.037	6.55IleIle: 6.55 ± 0.12	7.277IleLys: 7.277 ± 0.106	7.246IleLeu: 7.246 ± 0.125	1.826IleMet: 1.826 ± 0.054	5.435IleAsn: 5.435 ± 0.106	3.062IlePro: 3.062 ± 0.071	2.428IleGln: 2.428 ± 0.057	2.834IleArg: 2.834 ± 0.07	5.407IleSer: 5.407 ± 0.102	4.611IleThr: 4.611 ± 0.082	4.429IleVal: 4.429 ± 0.09	0.432IleTrp: 0.432 ± 0.028	2.981IleTyr: 2.981 ± 0.067	0.0IleXaa: 0.0 ± 0.0
Lys	5.699LysAla: 5.699 ± 0.13	0.993LysCys: 0.993 ± 0.044	5.306LysAsp: 5.306 ± 0.095	6.923LysGlu: 6.923 ± 0.116	3.686LysPhe: 3.686 ± 0.084	4.393LysGly: 4.393 ± 0.076	1.403LysHis: 1.403 ± 0.046	7.722LysIle: 7.722 ± 0.127	8.023LysLys: 8.023 ± 0.133	7.851LysLeu: 7.851 ± 0.105	2.31LysMet: 2.31 ± 0.068	6.509LysAsn: 6.509 ± 0.108	2.931LysPro: 2.931 ± 0.078	3.08LysGln: 3.08 ± 0.073	3.187LysArg: 3.187 ± 0.072	4.989LysSer: 4.989 ± 0.082	5.271LysThr: 5.271 ± 0.097	4.764LysVal: 4.764 ± 0.081	0.587LysTrp: 0.587 ± 0.032	4.118LysTyr: 4.118 ± 0.095	0.0LysXaa: 0.0 ± 0.0
Leu	6.326LeuAla: 6.326 ± 0.114	1.111LeuCys: 1.111 ± 0.049	4.936LeuAsp: 4.936 ± 0.092	5.896LeuGlu: 5.896 ± 0.111	3.752LeuPhe: 3.752 ± 0.101	5.424LeuGly: 5.424 ± 0.09	1.458LeuHis: 1.458 ± 0.052	7.061LeuIle: 7.061 ± 0.119	8.556LeuLys: 8.556 ± 0.122	7.275LeuLeu: 7.275 ± 0.127	2.238LeuMet: 2.238 ± 0.063	5.88LeuAsn: 5.88 ± 0.095	3.377LeuPro: 3.377 ± 0.071	2.871LeuGln: 2.871 ± 0.07	3.193LeuArg: 3.193 ± 0.075	6.139LeuSer: 6.139 ± 0.097	5.226LeuThr: 5.226 ± 0.107	4.546LeuVal: 4.546 ± 0.104	0.52LeuTrp: 0.52 ± 0.032	3.077LeuTyr: 3.077 ± 0.073	0.005LeuXaa: 0.005 ± 0.003
Met	1.806MetAla: 1.806 ± 0.054	0.27MetCys: 0.27 ± 0.023	1.33MetAsp: 1.33 ± 0.048	1.344MetGlu: 1.344 ± 0.051	1.116MetPhe: 1.116 ± 0.042	1.431MetGly: 1.431 ± 0.052	0.414MetHis: 0.414 ± 0.023	1.71MetIle: 1.71 ± 0.054	2.218MetLys: 2.218 ± 0.059	2.253MetLeu: 2.253 ± 0.06	0.651MetMet: 0.651 ± 0.035	1.603MetAsn: 1.603 ± 0.057	1.18MetPro: 1.18 ± 0.046	1.127MetGln: 1.127 ± 0.05	0.921MetArg: 0.921 ± 0.038	1.693MetSer: 1.693 ± 0.055	1.645MetThr: 1.645 ± 0.059	1.273MetVal: 1.273 ± 0.044	0.158MetTrp: 0.158 ± 0.017	0.861MetTyr: 0.861 ± 0.039	0.002MetXaa: 0.002 ± 0.001
Asn	4.087AsnAla: 4.087 ± 0.098	0.826AsnCys: 0.826 ± 0.042	3.212AsnAsp: 3.212 ± 0.076	3.608AsnGlu: 3.608 ± 0.062	3.041AsnPhe: 3.041 ± 0.069	4.203AsnGly: 4.203 ± 0.119	0.844AsnHis: 0.844 ± 0.03	5.769AsnIle: 5.769 ± 0.117	5.492AsnLys: 5.492 ± 0.115	5.637AsnLeu: 5.637 ± 0.113	1.531AsnMet: 1.531 ± 0.047	4.295AsnAsn: 4.295 ± 0.118	2.65AsnPro: 2.65 ± 0.068	1.785AsnGln: 1.785 ± 0.053	1.918AsnArg: 1.918 ± 0.063	4.256AsnSer: 4.256 ± 0.111	3.162AsnThr: 3.162 ± 0.098	3.433AsnVal: 3.433 ± 0.069	0.632AsnTrp: 0.632 ± 0.036	2.903AsnTyr: 2.903 ± 0.071	0.002AsnXaa: 0.002 ± 0.002
Pro	2.358ProAla: 2.358 ± 0.069	0.493ProCys: 0.493 ± 0.029	2.508ProAsp: 2.508 ± 0.064	2.884ProGlu: 2.884 ± 0.076	1.459ProPhe: 1.459 ± 0.054	1.331ProGly: 1.331 ± 0.049	0.595ProHis: 0.595 ± 0.032	2.061ProIle: 2.061 ± 0.052	2.43ProLys: 2.43 ± 0.062	2.669ProLeu: 2.669 ± 0.065	0.726ProMet: 0.726 ± 0.034	1.985ProAsn: 1.985 ± 0.062	0.985ProPro: 0.985 ± 0.05	1.443ProGln: 1.443 ± 0.055	0.89ProArg: 0.89 ± 0.034	2.091ProSer: 2.091 ± 0.052	1.401ProThr: 1.401 ± 0.056	2.786ProVal: 2.786 ± 0.075	0.208ProTrp: 0.208 ± 0.021	1.467ProTyr: 1.467 ± 0.052	0.0ProXaa: 0.0 ± 0.0
Gln	2.47GlnAla: 2.47 ± 0.068	0.325GlnCys: 0.325 ± 0.024	1.618GlnAsp: 1.618 ± 0.049	2.264GlnGlu: 2.264 ± 0.066	1.183GlnPhe: 1.183 ± 0.042	1.626GlnGly: 1.626 ± 0.047	0.448GlnHis: 0.448 ± 0.025	2.765GlnIle: 2.765 ± 0.068	3.198GlnLys: 3.198 ± 0.084	2.665GlnLeu: 2.665 ± 0.07	0.891GlnMet: 0.891 ± 0.033	2.411GlnAsn: 2.411 ± 0.061	0.922GlnPro: 0.922 ± 0.046	1.35GlnGln: 1.35 ± 0.062	1.256GlnArg: 1.256 ± 0.04	1.788GlnSer: 1.788 ± 0.053	1.907GlnThr: 1.907 ± 0.055	1.738GlnVal: 1.738 ± 0.053	0.233GlnTrp: 0.233 ± 0.018	1.311GlnTyr: 1.311 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	2.258ArgAla: 2.258 ± 0.067	0.432ArgCys: 0.432 ± 0.028	1.949ArgAsp: 1.949 ± 0.058	2.684ArgGlu: 2.684 ± 0.068	1.668ArgPhe: 1.668 ± 0.048	1.941ArgGly: 1.941 ± 0.061	0.607ArgHis: 0.607 ± 0.035	2.929ArgIle: 2.929 ± 0.067	3.138ArgLys: 3.138 ± 0.079	3.338ArgLeu: 3.338 ± 0.086	0.861ArgMet: 0.861 ± 0.041	2.108ArgAsn: 2.108 ± 0.059	1.099ArgPro: 1.099 ± 0.044	1.291ArgGln: 1.291 ± 0.041	1.35ArgArg: 1.35 ± 0.048	1.668ArgSer: 1.668 ± 0.055	1.751ArgThr: 1.751 ± 0.058	2.094ArgVal: 2.094 ± 0.062	0.268ArgTrp: 0.268 ± 0.02	1.442ArgTyr: 1.442 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	4.029SerAla: 4.029 ± 0.088	0.707SerCys: 0.707 ± 0.033	3.636SerAsp: 3.636 ± 0.076	3.831SerGlu: 3.831 ± 0.086	2.737SerPhe: 2.737 ± 0.071	4.287SerGly: 4.287 ± 0.093	0.955SerHis: 0.955 ± 0.034	4.538SerIle: 4.538 ± 0.084	4.866SerLys: 4.866 ± 0.082	5.154SerLeu: 5.154 ± 0.09	1.445SerMet: 1.445 ± 0.048	3.311SerAsn: 3.311 ± 0.079	1.859SerPro: 1.859 ± 0.059	2.199SerGln: 2.199 ± 0.056	2.083SerArg: 2.083 ± 0.054	4.028SerSer: 4.028 ± 0.088	3.026SerThr: 3.026 ± 0.075	3.855SerVal: 3.855 ± 0.067	0.46SerTrp: 0.46 ± 0.024	2.409SerTyr: 2.409 ± 0.066	0.0SerXaa: 0.0 ± 0.0
Thr	4.011ThrAla: 4.011 ± 0.091	0.657ThrCys: 0.657 ± 0.037	3.505ThrAsp: 3.505 ± 0.131	3.304ThrGlu: 3.304 ± 0.081	2.338ThrPhe: 2.338 ± 0.066	3.725ThrGly: 3.725 ± 0.078	0.805ThrHis: 0.805 ± 0.037	3.951ThrIle: 3.951 ± 0.086	4.042ThrLys: 4.042 ± 0.079	5.209ThrLeu: 5.209 ± 0.103	1.122ThrMet: 1.122 ± 0.04	2.806ThrAsn: 2.806 ± 0.078	2.233ThrPro: 2.233 ± 0.07	1.678ThrGln: 1.678 ± 0.049	1.791ThrArg: 1.791 ± 0.05	3.269ThrSer: 3.269 ± 0.083	2.795ThrThr: 2.795 ± 0.077	3.801ThrVal: 3.801 ± 0.074	0.297ThrTrp: 0.297 ± 0.026	2.093ThrTyr: 2.093 ± 0.058	0.0ThrXaa: 0.0 ± 0.0
Val	3.78ValAla: 3.78 ± 0.086	0.935ValCys: 0.935 ± 0.043	3.497ValAsp: 3.497 ± 0.07	3.998ValGlu: 3.998 ± 0.093	2.692ValPhe: 2.692 ± 0.071	3.12ValGly: 3.12 ± 0.075	0.88ValHis: 0.88 ± 0.034	5.002ValIle: 5.002 ± 0.111	5.673ValLys: 5.673 ± 0.092	5.532ValLeu: 5.532 ± 0.095	1.589ValMet: 1.589 ± 0.049	3.634ValAsn: 3.634 ± 0.085	2.091ValPro: 2.091 ± 0.059	1.884ValGln: 1.884 ± 0.064	2.058ValArg: 2.058 ± 0.062	3.951ValSer: 3.951 ± 0.079	3.418ValThr: 3.418 ± 0.083	3.772ValVal: 3.772 ± 0.087	0.414ValTrp: 0.414 ± 0.027	2.261ValTyr: 2.261 ± 0.065	0.003ValXaa: 0.003 ± 0.002
Trp	0.485TrpAla: 0.485 ± 0.027	0.105TrpCys: 0.105 ± 0.013	0.432TrpAsp: 0.432 ± 0.024	0.549TrpGlu: 0.549 ± 0.032	0.354TrpPhe: 0.354 ± 0.023	0.571TrpGly: 0.571 ± 0.029	0.169TrpHis: 0.169 ± 0.015	0.484TrpIle: 0.484 ± 0.03	0.51TrpLys: 0.51 ± 0.029	0.755TrpLeu: 0.755 ± 0.032	0.203TrpMet: 0.203 ± 0.019	0.414TrpAsn: 0.414 ± 0.027	0.133TrpPro: 0.133 ± 0.016	0.264TrpGln: 0.264 ± 0.024	0.25TrpArg: 0.25 ± 0.021	0.39TrpSer: 0.39 ± 0.023	0.331TrpThr: 0.331 ± 0.021	0.448TrpVal: 0.448 ± 0.029	0.092TrpTrp: 0.092 ± 0.013	0.303TrpTyr: 0.303 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.467TyrAla: 2.467 ± 0.062	0.635TyrCys: 0.635 ± 0.035	2.52TyrAsp: 2.52 ± 0.075	2.581TyrGlu: 2.581 ± 0.067	2.1TyrPhe: 2.1 ± 0.064	2.392TyrGly: 2.392 ± 0.067	0.626TyrHis: 0.626 ± 0.03	3.085TyrIle: 3.085 ± 0.076	3.689TyrLys: 3.689 ± 0.083	3.556TyrLeu: 3.556 ± 0.081	1.028TyrMet: 1.028 ± 0.041	3.121TyrAsn: 3.121 ± 0.084	1.523TyrPro: 1.523 ± 0.062	1.133TyrGln: 1.133 ± 0.045	1.522TyrArg: 1.522 ± 0.048	2.757TyrSer: 2.757 ± 0.07	2.233TyrThr: 2.233 ± 0.068	2.239TyrVal: 2.239 ± 0.058	0.331TyrTrp: 0.331 ± 0.022	1.801TyrTyr: 1.801 ± 0.06	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.003XaaCys: 0.003 ± 0.002	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.003XaaLys: 0.003 ± 0.002	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.002XaaThr: 0.002 ± 0.002	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.023XaaXaa: 0.023 ± 0.008

Statistics based on 2311 proteins (640807 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---