Luteimonas sp. FCS-9
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3200 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H1ASJ2|A0A0H1ASJ2_9GAMM DUF3298 domain-containing protein OS=Luteimonas sp. FCS-9 OX=1547516 GN=WQ56_04070 PE=4 SV=1
MM1 pKa = 7.5 HH2 pKa = 7.32 IAAPDD7 pKa = 3.64 ALRR10 pKa = 11.84 AAFLPPPHH18 pKa = 7.35 DD19 pKa = 4.08 PQPAAKK25 pKa = 10.08 ADD27 pKa = 3.99 TPEE30 pKa = 3.61 QTPFVRR36 pKa = 11.84 GEE38 pKa = 4.18 GDD40 pKa = 3.02 RR41 pKa = 11.84 HH42 pKa = 5.04 TVDD45 pKa = 4.65 PNDD48 pKa = 3.66 IQQDD52 pKa = 4.35 GYY54 pKa = 11.19 GSCAVLSTLHH64 pKa = 6.76 TIAQHH69 pKa = 6.35 DD70 pKa = 4.03 PSVIQDD76 pKa = 3.99 MIQDD80 pKa = 4.22 NGDD83 pKa = 2.95 GTYY86 pKa = 10.17 TVTFQEE92 pKa = 4.39 KK93 pKa = 10.25 VEE95 pKa = 3.98 ILGFTFFKK103 pKa = 10.34 PVEE106 pKa = 4.3 VTVSGPFTGGAANPADD122 pKa = 3.37 VGANGEE128 pKa = 4.47 EE129 pKa = 4.15 VWPAIIEE136 pKa = 3.88 AAYY139 pKa = 8.84 AQQYY143 pKa = 10.14 KK144 pKa = 10.82 GGDD147 pKa = 3.38 LTYY150 pKa = 9.32 EE151 pKa = 4.21 TGVMPADD158 pKa = 3.35 VMEE161 pKa = 5.74 RR162 pKa = 11.84 ILGADD167 pKa = 3.3 AQTAGTGEE175 pKa = 4.12 VSAEE179 pKa = 3.87 QMSQRR184 pKa = 11.84 LADD187 pKa = 3.98 GEE189 pKa = 4.41 ATVAWTPGFKK199 pKa = 10.49 DD200 pKa = 5.25 DD201 pKa = 5.22 DD202 pKa = 4.93 GNWLPEE208 pKa = 3.81 ITEE211 pKa = 4.06 EE212 pKa = 3.93 QQALIEE218 pKa = 4.55 HH219 pKa = 6.18 YY220 pKa = 10.58 GIAGGHH226 pKa = 6.75 AYY228 pKa = 9.82 AVSEE232 pKa = 4.4 VIPAGTSYY240 pKa = 11.47 VDD242 pKa = 3.35 PTTGEE247 pKa = 4.22 SVVASEE253 pKa = 5.49 DD254 pKa = 4.14 VIVLDD259 pKa = 4.49 NPWGSSDD266 pKa = 3.76 VVMPYY271 pKa = 10.55 SDD273 pKa = 3.65 YY274 pKa = 11.07 QEE276 pKa = 4.17 VYY278 pKa = 10.1 GWVSSAATEE287 pKa = 3.96
Molecular weight: 30.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.884
IPC_protein 3.872
Toseland 3.668
ProMoST 3.999
Dawson 3.846
Bjellqvist 4.024
Wikipedia 3.757
Rodwell 3.706
Grimsley 3.579
Solomon 3.846
Lehninger 3.808
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.986
Patrickios 1.227
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.895
Protein with the highest isoelectric point:
>tr|A0A0H1ANH3|A0A0H1ANH3_9GAMM Uncharacterized protein OS=Luteimonas sp. FCS-9 OX=1547516 GN=WQ56_08935 PE=4 SV=1
MM1 pKa = 7.86 PLPSATRR8 pKa = 11.84 APARR12 pKa = 11.84 CRR14 pKa = 11.84 RR15 pKa = 11.84 PARR18 pKa = 11.84 PPRR21 pKa = 11.84 PRR23 pKa = 11.84 PRR25 pKa = 11.84 PRR27 pKa = 11.84 PGPPGCRR34 pKa = 11.84 TSRR37 pKa = 11.84 PRR39 pKa = 11.84 AWPRR43 pKa = 11.84 PARR46 pKa = 11.84 RR47 pKa = 11.84 TPRR50 pKa = 11.84 AASPAGRR57 pKa = 11.84 PGGNGRR63 pKa = 11.84 AVRR66 pKa = 11.84 LRR68 pKa = 11.84 TDD70 pKa = 3.08 AAA72 pKa = 3.9
Molecular weight: 7.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.382
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.828
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.176
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3200
0
3200
1065100
41
2810
332.8
35.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.355 ± 0.08
0.766 ± 0.011
6.438 ± 0.038
4.982 ± 0.04
3.286 ± 0.029
8.984 ± 0.038
2.222 ± 0.022
3.843 ± 0.028
1.933 ± 0.033
10.709 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.989 ± 0.019
2.089 ± 0.03
5.625 ± 0.041
3.395 ± 0.027
8.453 ± 0.045
4.549 ± 0.032
4.93 ± 0.025
7.707 ± 0.037
1.562 ± 0.021
2.184 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here