Luteipulveratus halotolerans
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3827 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0L6CH81|A0A0L6CH81_9MICO Proteasome protein OS=Luteipulveratus halotolerans OX=1631356 GN=VV01_07880 PE=4 SV=1
MM1 pKa = 7.86 SEE3 pKa = 4.36 LEE5 pKa = 4.11 YY6 pKa = 10.25 PADD9 pKa = 3.42 LRR11 pKa = 11.84 YY12 pKa = 8.7 TGDD15 pKa = 3.84 HH16 pKa = 6.32 EE17 pKa = 4.42 WVADD21 pKa = 3.65 KK22 pKa = 11.31 GDD24 pKa = 3.45 GVVRR28 pKa = 11.84 VGITAYY34 pKa = 10.48 AQDD37 pKa = 3.56 ALGDD41 pKa = 3.79 VVYY44 pKa = 11.12 VSLPAVGDD52 pKa = 3.64 AVTAGDD58 pKa = 3.41 SCGEE62 pKa = 4.14 VEE64 pKa = 4.44 STKK67 pKa = 10.7 SVSDD71 pKa = 4.11 LYY73 pKa = 11.49 SPLEE77 pKa = 4.22 GEE79 pKa = 4.51 VVAVNDD85 pKa = 4.57 ALDD88 pKa = 4.04 GAPEE92 pKa = 4.13 QVNSAPYY99 pKa = 9.06 GDD101 pKa = 3.08 GWMFEE106 pKa = 4.39 MKK108 pKa = 10.45 VADD111 pKa = 3.91 PAAIEE116 pKa = 4.13 ALLDD120 pKa = 3.48 VEE122 pKa = 5.2 AYY124 pKa = 9.4 KK125 pKa = 10.06 ATLGG129 pKa = 3.68
Molecular weight: 13.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.694
IPC_protein 3.668
Toseland 3.465
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.605
Rodwell 3.503
Grimsley 3.376
Solomon 3.656
Lehninger 3.605
Nozaki 3.795
DTASelect 3.999
Thurlkill 3.528
EMBOSS 3.617
Sillero 3.795
Patrickios 0.896
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.73
Protein with the highest isoelectric point:
>tr|A0A0L6CM08|A0A0L6CM08_9MICO ATP-binding protein OS=Luteipulveratus halotolerans OX=1631356 GN=VV01_18400 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.97 KK16 pKa = 9.33 HH17 pKa = 4.25 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AVLASRR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.74 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3827
0
3827
1160312
37
2006
303.2
32.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.987 ± 0.048
0.726 ± 0.011
6.66 ± 0.04
5.414 ± 0.043
2.613 ± 0.023
8.98 ± 0.039
2.306 ± 0.018
3.514 ± 0.027
2.166 ± 0.03
9.947 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.939 ± 0.016
1.69 ± 0.019
5.469 ± 0.029
3.144 ± 0.022
7.725 ± 0.043
5.566 ± 0.029
6.267 ± 0.035
9.33 ± 0.039
1.562 ± 0.018
1.998 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here