Halorubrum vacuolatum (Natronobacterium vacuolatum)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halorubrum

Average proteome isoelectric point is 5.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3371 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A238W4I5|A0A238W4I5_HALVU Dimethylglycine oxidase OS=Halorubrum vacuolatum OX=63740 GN=SAMN06264855_105105 PE=3 SV=1
MM1 pKa = 7.54FPRR4 pKa = 11.84EE5 pKa = 3.92YY6 pKa = 10.29RR7 pKa = 11.84GVAFAVALFVLVQLGALALVPSFAEE32 pKa = 3.89SGYY35 pKa = 10.73QPVDD39 pKa = 3.71DD40 pKa = 5.12PQDD43 pKa = 3.72PVNSLIYY50 pKa = 9.85IVAIVVMTGFMLAAFRR66 pKa = 11.84YY67 pKa = 8.38EE68 pKa = 4.12LDD70 pKa = 2.84WAIRR74 pKa = 11.84LLIVGVSGWLSWYY87 pKa = 9.65VFSAILPPLLAAIPAIAVAVGLLVHH112 pKa = 6.31PEE114 pKa = 3.7WYY116 pKa = 10.47VIDD119 pKa = 3.83SAGVLMGAGAAGLFGITFGILPALILLVFLAIYY152 pKa = 9.95DD153 pKa = 4.47AISVYY158 pKa = 9.3GTEE161 pKa = 4.41HH162 pKa = 6.88MLTLAEE168 pKa = 4.67GVMDD172 pKa = 4.42LNIPVVLVIPTTLSYY187 pKa = 11.5SLLEE191 pKa = 4.81DD192 pKa = 4.5DD193 pKa = 5.41FSGAADD199 pKa = 3.4VHH201 pKa = 6.56EE202 pKa = 5.21GDD204 pKa = 5.01DD205 pKa = 3.72EE206 pKa = 5.0GEE208 pKa = 3.87EE209 pKa = 4.41GEE211 pKa = 4.65PIKK214 pKa = 10.89GDD216 pKa = 3.32EE217 pKa = 4.73DD218 pKa = 4.64GEE220 pKa = 4.34ITADD224 pKa = 3.54SDD226 pKa = 4.9DD227 pKa = 5.3EE228 pKa = 4.24ITADD232 pKa = 5.0SDD234 pKa = 4.81DD235 pKa = 3.98EE236 pKa = 5.96HH237 pKa = 7.03DD238 pKa = 4.23TADD241 pKa = 4.74GDD243 pKa = 5.7DD244 pKa = 4.11PDD246 pKa = 4.73EE247 pKa = 5.07DD248 pKa = 3.95PSRR251 pKa = 11.84DD252 pKa = 3.29AFFIGLGDD260 pKa = 3.74AVIPAVLVASAATFSPAPHH279 pKa = 6.7LSVPVVGLNLPALLAMVGIVAGLLVLMSWVMKK311 pKa = 10.23GRR313 pKa = 11.84AHH315 pKa = 7.57AGLPLLNGGAIGGYY329 pKa = 10.39LIGSLLAGVPLIEE342 pKa = 4.71AVGLGPFVV350 pKa = 3.38

Molecular weight: 36.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A238VL29|A0A238VL29_HALVU C2H2-type zinc finger OS=Halorubrum vacuolatum OX=63740 GN=SAMN06264855_10358 PE=4 SV=1
MM1 pKa = 7.36ATLLCRR7 pKa = 11.84RR8 pKa = 11.84CFPGFFSEE16 pKa = 5.14LPTGSGDD23 pKa = 3.23RR24 pKa = 11.84SIRR27 pKa = 11.84YY28 pKa = 8.26NFSQCLVLEE37 pKa = 4.32YY38 pKa = 10.95SCIRR42 pKa = 11.84RR43 pKa = 11.84FALHH47 pKa = 6.69LVKK50 pKa = 10.6LPNVRR55 pKa = 11.84APSSVSQHH63 pKa = 5.84TIYY66 pKa = 10.84SIGPGQNLLDD76 pKa = 4.64GIKK79 pKa = 10.28QIVRR83 pKa = 11.84GHH85 pKa = 5.29TLSTEE90 pKa = 3.94NTGGNRR96 pKa = 11.84LARR99 pKa = 11.84DD100 pKa = 3.68GVSAPKK106 pKa = 10.42LDD108 pKa = 3.78VKK110 pKa = 8.69YY111 pKa = 10.34HH112 pKa = 6.25RR113 pKa = 11.84SHH115 pKa = 7.19RR116 pKa = 11.84PTASPPKK123 pKa = 9.33SHH125 pKa = 5.5IHH127 pKa = 5.07GRR129 pKa = 11.84AVATSIKK136 pKa = 9.46CTIPNAPHH144 pKa = 6.99RR145 pKa = 11.84FSNGSDD151 pKa = 3.29GYY153 pKa = 9.72TIDD156 pKa = 5.59LRR158 pKa = 11.84QQAAGNTARR167 pKa = 11.84EE168 pKa = 4.03SDD170 pKa = 3.46KK171 pKa = 11.37LGADD175 pKa = 3.05RR176 pKa = 11.84RR177 pKa = 11.84VITGG181 pKa = 3.32

Molecular weight: 19.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3371

0

3371

1002170

26

2727

297.3

32.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.899 ± 0.067

0.716 ± 0.014

8.415 ± 0.06

8.695 ± 0.061

3.298 ± 0.03

8.474 ± 0.046

2.062 ± 0.023

4.664 ± 0.032

1.947 ± 0.034

8.727 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.71 ± 0.021

2.307 ± 0.027

4.746 ± 0.037

2.217 ± 0.029

6.924 ± 0.046

5.241 ± 0.032

6.499 ± 0.047

8.723 ± 0.04

1.103 ± 0.018

2.633 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski