Peristrophe mosaic virus
Average proteome isoelectric point is 8.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K4Q718|K4Q718_9GEMI Movement protein BC1 OS=Peristrophe mosaic virus OX=1188812 GN=BC1 PE=3 SV=1
MM1 pKa = 7.56 NSQLANPPSAFNYY14 pKa = 9.74 IEE16 pKa = 4.05 SHH18 pKa = 6.24 RR19 pKa = 11.84 DD20 pKa = 3.22 EE21 pKa = 4.7 YY22 pKa = 11.29 QLSHH26 pKa = 7.37 DD27 pKa = 4.31 LNEE30 pKa = 5.35 IILQFPSTTSQFTARR45 pKa = 11.84 LSRR48 pKa = 11.84 SCMKK52 pKa = 9.84 IDD54 pKa = 3.26 HH55 pKa = 6.45 CVIEE59 pKa = 4.31 YY60 pKa = 9.79 RR61 pKa = 11.84 QQVPINASGTVIVEE75 pKa = 3.83 IHH77 pKa = 7.14 DD78 pKa = 3.91 KK79 pKa = 11.26 RR80 pKa = 11.84 MTDD83 pKa = 3.35 NEE85 pKa = 4.35 SLQACWTFPIRR96 pKa = 11.84 CNIDD100 pKa = 2.89 LHH102 pKa = 6.29 YY103 pKa = 10.06 FSCSFFSLKK112 pKa = 10.84 DD113 pKa = 3.8 PIPWKK118 pKa = 10.22 LYY120 pKa = 10.12 YY121 pKa = 9.87 RR122 pKa = 11.84 VSDD125 pKa = 3.88 TNVHH129 pKa = 5.9 QGTHH133 pKa = 4.64 FAKK136 pKa = 10.89 FKK138 pKa = 11.14 GKK140 pKa = 10.52 LKK142 pKa = 10.82 LSTAKK147 pKa = 10.37 HH148 pKa = 5.69 SVDD151 pKa = 2.95 IPFRR155 pKa = 11.84 APTVKK160 pKa = 10.11 ILSKK164 pKa = 10.52 QFSSKK169 pKa = 10.84 DD170 pKa = 3.12 VDD172 pKa = 4.45 FSHH175 pKa = 7.04 VGYY178 pKa = 10.7 GKK180 pKa = 9.46 WEE182 pKa = 3.93 RR183 pKa = 11.84 KK184 pKa = 9.53 LIRR187 pKa = 11.84 STSMTRR193 pKa = 11.84 LGQHH197 pKa = 6.25 GPIEE201 pKa = 4.36 LNPGEE206 pKa = 4.39 SWASRR211 pKa = 11.84 STIGLTPTNEE221 pKa = 3.88 EE222 pKa = 4.04 SDD224 pKa = 4.17 VEE226 pKa = 4.38 NAIHH230 pKa = 7.81 PYY232 pKa = 10.4 RR233 pKa = 11.84 EE234 pKa = 4.19 LNKK237 pKa = 10.61 LGTSLLDD244 pKa = 4.08 PGEE247 pKa = 4.34 SASVVGAQRR256 pKa = 11.84 AQSNLTISMAQLNEE270 pKa = 3.87 IVRR273 pKa = 11.84 TAAQEE278 pKa = 4.39 CINNNCIPSQPKK290 pKa = 9.67 SLKK293 pKa = 10.34
Molecular weight: 33.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.15
IPC2_protein 7.454
IPC_protein 7.38
Toseland 7.117
ProMoST 8.068
Dawson 8.053
Bjellqvist 8.419
Wikipedia 7.98
Rodwell 8.068
Grimsley 7.19
Solomon 8.141
Lehninger 8.17
Nozaki 8.682
DTASelect 8.17
Thurlkill 8.229
EMBOSS 8.287
Sillero 8.551
Patrickios 4.38
IPC_peptide 8.141
IPC2_peptide 7.541
IPC2.peptide.svr19 7.497
Protein with the highest isoelectric point:
>tr|K4Q718|K4Q718_9GEMI Movement protein BC1 OS=Peristrophe mosaic virus OX=1188812 GN=BC1 PE=3 SV=1
MM1 pKa = 7.28 YY2 pKa = 9.93 SSKK5 pKa = 10.47 YY6 pKa = 10.16 RR7 pKa = 11.84 RR8 pKa = 11.84 DD9 pKa = 2.83 QSTYY13 pKa = 6.64 QRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 YY18 pKa = 8.63 SRR20 pKa = 11.84 NYY22 pKa = 8.32 VFKK25 pKa = 9.7 RR26 pKa = 11.84 TSGSKK31 pKa = 8.56 RR32 pKa = 11.84 HH33 pKa = 5.22 EE34 pKa = 4.34 GKK36 pKa = 9.99 RR37 pKa = 11.84 RR38 pKa = 11.84 HH39 pKa = 5.02 SQGVKK44 pKa = 10.35 DD45 pKa = 3.97 YY46 pKa = 9.63 DD47 pKa = 3.61 HH48 pKa = 7.02 CVKK51 pKa = 10.44 MMGQHH56 pKa = 5.82 INEE59 pKa = 4.21 NQFGPEE65 pKa = 3.18 FAMAHH70 pKa = 5.12 NTSISTFINYY80 pKa = 7.74 PHH82 pKa = 7.31 LGKK85 pKa = 10.14 NLPNRR90 pKa = 11.84 SRR92 pKa = 11.84 SYY94 pKa = 10.92 IKK96 pKa = 10.58 LKK98 pKa = 10.35 RR99 pKa = 11.84 LRR101 pKa = 11.84 FKK103 pKa = 10.51 GTVKK107 pKa = 10.13 IEE109 pKa = 3.97 RR110 pKa = 11.84 VPVDD114 pKa = 3.41 VNMDD118 pKa = 3.4 GLIPKK123 pKa = 9.85 IEE125 pKa = 4.28 GVFSLVIVVDD135 pKa = 4.77 RR136 pKa = 11.84 KK137 pKa = 9.78 PHH139 pKa = 5.83 LSSSGSLHH147 pKa = 6.14 TFDD150 pKa = 4.83 EE151 pKa = 4.77 LFGARR156 pKa = 11.84 IHH158 pKa = 5.92 SHH160 pKa = 5.9 GNLTISSSLKK170 pKa = 8.43 DD171 pKa = 3.18 RR172 pKa = 11.84 FYY174 pKa = 10.68 IRR176 pKa = 11.84 HH177 pKa = 4.4 VFKK180 pKa = 10.81 RR181 pKa = 11.84 VLSVEE186 pKa = 4.32 KK187 pKa = 10.17 DD188 pKa = 2.97 SMMIDD193 pKa = 3.18 IEE195 pKa = 4.54 GSTNLSNRR203 pKa = 11.84 RR204 pKa = 11.84 YY205 pKa = 10.86 NMWSTFKK212 pKa = 11.16 DD213 pKa = 3.61 LDD215 pKa = 3.8 HH216 pKa = 7.43 DD217 pKa = 4.4 SCNGVYY223 pKa = 10.88 DD224 pKa = 4.73 NISKK228 pKa = 10.35 NAILIYY234 pKa = 8.67 YY235 pKa = 9.04 CWMSDD240 pKa = 3.0 IVSKK244 pKa = 11.17 ASTYY248 pKa = 10.84 VSFDD252 pKa = 3.29 LSYY255 pKa = 11.44 VGG257 pKa = 4.98
Molecular weight: 29.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.917
IPC2_protein 9.341
IPC_protein 9.37
Toseland 9.823
ProMoST 9.619
Dawson 10.087
Bjellqvist 9.809
Wikipedia 10.292
Rodwell 10.379
Grimsley 10.189
Solomon 10.116
Lehninger 10.072
Nozaki 9.853
DTASelect 9.794
Thurlkill 9.926
EMBOSS 10.248
Sillero 10.014
Patrickios 5.575
IPC_peptide 10.116
IPC2_peptide 8.521
IPC2.peptide.svr19 8.323
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
550
257
293
275.0
31.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.818 ± 1.34
1.818 ± 0.466
5.273 ± 0.682
4.364 ± 0.895
4.364 ± 0.219
4.909 ± 0.664
4.182 ± 0.349
6.909 ± 0.211
6.545 ± 0.606
7.091 ± 0.619
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.545 ± 0.684
5.818 ± 0.013
4.0 ± 1.192
3.636 ± 1.21
6.727 ± 1.311
11.818 ± 0.453
5.091 ± 0.858
5.818 ± 0.848
1.091 ± 0.224
4.182 ± 1.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here