Methanobacterium virus Drs3
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385AH44|A0A385AH44_9CAUD Portal protein OS=Methanobacterium virus Drs3 OX=1430441 GN=Drs3_00022 PE=4 SV=1
MM1 pKa = 7.02 KK2 pKa = 9.58 TCEE5 pKa = 4.01 YY6 pKa = 9.69 CGGMEE11 pKa = 4.97 FMFEE15 pKa = 4.12 EE16 pKa = 4.48 EE17 pKa = 4.14 TGRR20 pKa = 11.84 EE21 pKa = 4.0 YY22 pKa = 10.75 CTTCGYY28 pKa = 8.26 YY29 pKa = 10.01 TGVVDD34 pKa = 4.84 EE35 pKa = 5.24 SYY37 pKa = 11.35 EE38 pKa = 4.15 EE39 pKa = 4.15 YY40 pKa = 8.82 HH41 pKa = 6.37 TLMGDD46 pKa = 3.58 YY47 pKa = 10.92
Molecular weight: 5.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.014
IPC2_protein 4.101
IPC_protein 3.884
Toseland 3.757
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.706
Rodwell 3.732
Grimsley 3.681
Solomon 3.795
Lehninger 3.745
Nozaki 3.973
DTASelect 3.999
Thurlkill 3.783
EMBOSS 3.719
Sillero 3.999
Patrickios 0.006
IPC_peptide 3.808
IPC2_peptide 3.986
IPC2.peptide.svr19 3.915
Protein with the highest isoelectric point:
>tr|A0A385AH85|A0A385AH85_9CAUD Uncharacterized protein OS=Methanobacterium virus Drs3 OX=1430441 GN=Drs3_00001 PE=4 SV=1
MM1 pKa = 7.45 AAGYY5 pKa = 8.15 TIITHH10 pKa = 4.98 IQKK13 pKa = 10.76 DD14 pKa = 4.06 KK15 pKa = 11.43 LPTALDD21 pKa = 3.58 RR22 pKa = 11.84 VVDD25 pKa = 3.98 RR26 pKa = 11.84 ALDD29 pKa = 4.07 LGQMEE34 pKa = 5.12 LLGNLKK40 pKa = 10.33 RR41 pKa = 11.84 NSPVDD46 pKa = 3.38 EE47 pKa = 4.92 GNLQNSWFPLGRR59 pKa = 11.84 SRR61 pKa = 11.84 RR62 pKa = 11.84 EE63 pKa = 3.47 RR64 pKa = 11.84 LVRR67 pKa = 11.84 SSAKK71 pKa = 9.28 YY72 pKa = 10.63 AKK74 pKa = 9.72 FVNDD78 pKa = 3.39 GTGIYY83 pKa = 9.99 GPRR86 pKa = 11.84 GKK88 pKa = 10.9 LITPKK93 pKa = 9.62 TKK95 pKa = 9.92 KK96 pKa = 10.48 ALAFEE101 pKa = 4.67 YY102 pKa = 9.82 KK103 pKa = 10.4 GAMVVVKK110 pKa = 9.49 SVKK113 pKa = 9.9 GIKK116 pKa = 8.03 PQRR119 pKa = 11.84 YY120 pKa = 7.33 VEE122 pKa = 3.95 KK123 pKa = 10.63 SINEE127 pKa = 3.97 TEE129 pKa = 3.94 KK130 pKa = 11.0 RR131 pKa = 11.84 GDD133 pKa = 3.43 EE134 pKa = 4.56 FIIRR138 pKa = 11.84 AAMEE142 pKa = 4.4 AKK144 pKa = 10.47 GLLL147 pKa = 3.75
Molecular weight: 16.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.108
IPC2_protein 9.399
IPC_protein 9.414
Toseland 10.306
ProMoST 9.853
Dawson 10.423
Bjellqvist 10.028
Wikipedia 10.555
Rodwell 10.979
Grimsley 10.467
Solomon 10.452
Lehninger 10.438
Nozaki 10.262
DTASelect 10.028
Thurlkill 10.292
EMBOSS 10.672
Sillero 10.335
Patrickios 10.701
IPC_peptide 10.452
IPC2_peptide 8.434
IPC2.peptide.svr19 8.547
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
39
0
39
10464
43
1403
268.3
30.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.288 ± 0.728
0.898 ± 0.107
5.753 ± 0.393
7.97 ± 0.724
3.823 ± 0.189
7.502 ± 0.373
1.577 ± 0.158
6.68 ± 0.241
7.617 ± 0.54
8.582 ± 0.266
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.485 ± 0.226
5.419 ± 0.27
3.937 ± 0.265
3.087 ± 0.19
4.654 ± 0.365
6.135 ± 0.356
6.04 ± 0.399
6.355 ± 0.381
1.386 ± 0.157
3.813 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here