Methanobacterium virus Drs3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A385AH44|A0A385AH44_9CAUD Portal protein OS=Methanobacterium virus Drs3 OX=1430441 GN=Drs3_00022 PE=4 SV=1
MM1 pKa = 7.02KK2 pKa = 9.58TCEE5 pKa = 4.01YY6 pKa = 9.69CGGMEE11 pKa = 4.97FMFEE15 pKa = 4.12EE16 pKa = 4.48EE17 pKa = 4.14TGRR20 pKa = 11.84EE21 pKa = 4.0YY22 pKa = 10.75CTTCGYY28 pKa = 8.26YY29 pKa = 10.01TGVVDD34 pKa = 4.84EE35 pKa = 5.24SYY37 pKa = 11.35EE38 pKa = 4.15EE39 pKa = 4.15YY40 pKa = 8.82HH41 pKa = 6.37TLMGDD46 pKa = 3.58YY47 pKa = 10.92

Molecular weight:
5.55 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A385AH85|A0A385AH85_9CAUD Uncharacterized protein OS=Methanobacterium virus Drs3 OX=1430441 GN=Drs3_00001 PE=4 SV=1
MM1 pKa = 7.45AAGYY5 pKa = 8.15TIITHH10 pKa = 4.98IQKK13 pKa = 10.76DD14 pKa = 4.06KK15 pKa = 11.43LPTALDD21 pKa = 3.58RR22 pKa = 11.84VVDD25 pKa = 3.98RR26 pKa = 11.84ALDD29 pKa = 4.07LGQMEE34 pKa = 5.12LLGNLKK40 pKa = 10.33RR41 pKa = 11.84NSPVDD46 pKa = 3.38EE47 pKa = 4.92GNLQNSWFPLGRR59 pKa = 11.84SRR61 pKa = 11.84RR62 pKa = 11.84EE63 pKa = 3.47RR64 pKa = 11.84LVRR67 pKa = 11.84SSAKK71 pKa = 9.28YY72 pKa = 10.63AKK74 pKa = 9.72FVNDD78 pKa = 3.39GTGIYY83 pKa = 9.99GPRR86 pKa = 11.84GKK88 pKa = 10.9LITPKK93 pKa = 9.62TKK95 pKa = 9.92KK96 pKa = 10.48ALAFEE101 pKa = 4.67YY102 pKa = 9.82KK103 pKa = 10.4GAMVVVKK110 pKa = 9.49SVKK113 pKa = 9.9GIKK116 pKa = 8.03PQRR119 pKa = 11.84YY120 pKa = 7.33VEE122 pKa = 3.95KK123 pKa = 10.63SINEE127 pKa = 3.97TEE129 pKa = 3.94KK130 pKa = 11.0RR131 pKa = 11.84GDD133 pKa = 3.43EE134 pKa = 4.56FIIRR138 pKa = 11.84AAMEE142 pKa = 4.4AKK144 pKa = 10.47GLLL147 pKa = 3.75

Molecular weight:
16.45 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

10464

43

1403

268.3

30.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.288 ± 0.728

0.898 ± 0.107

5.753 ± 0.393

7.97 ± 0.724

3.823 ± 0.189

7.502 ± 0.373

1.577 ± 0.158

6.68 ± 0.241

7.617 ± 0.54

8.582 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.226

5.419 ± 0.27

3.937 ± 0.265

3.087 ± 0.19

4.654 ± 0.365

6.135 ± 0.356

6.04 ± 0.399

6.355 ± 0.381

1.386 ± 0.157

3.813 ± 0.246

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski