Enterobacterial phage mEp390

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7PHC8|K7PHC8_9CAUD Uncharacterized protein OS=Enterobacterial phage mEp390 OX=1147158 GN=mEp390_058 PE=4 SV=1
MM1 pKa = 7.08TVVLTAKK8 pKa = 10.33QIEE11 pKa = 4.34DD12 pKa = 3.48LANFAKK18 pKa = 10.51EE19 pKa = 4.3DD20 pKa = 3.63GAPQYY25 pKa = 10.61TITTGTIPEE34 pKa = 4.51FEE36 pKa = 5.03ADD38 pKa = 3.8DD39 pKa = 4.54GEE41 pKa = 4.96IIPEE45 pKa = 4.03YY46 pKa = 9.54TGLIAYY52 pKa = 9.73SDD54 pKa = 3.97SLEE57 pKa = 4.52HH58 pKa = 7.2GVLQLDD64 pKa = 4.27DD65 pKa = 3.8

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7PJS8|K7PJS8_9CAUD Uncharacterized protein OS=Enterobacterial phage mEp390 OX=1147158 GN=mEp390_055 PE=4 SV=1
MM1 pKa = 7.08NQSQFQKK8 pKa = 10.95AAGLSAEE15 pKa = 4.28LAARR19 pKa = 11.84WFQPVTDD26 pKa = 3.77AMKK29 pKa = 10.59EE30 pKa = 3.84FGITKK35 pKa = 9.84PVDD38 pKa = 3.12QAMFIAQVGHH48 pKa = 6.44EE49 pKa = 4.38SAGFTLLVEE58 pKa = 4.31SFNYY62 pKa = 10.13RR63 pKa = 11.84IAALVNFIRR72 pKa = 11.84AGRR75 pKa = 11.84LTAEE79 pKa = 4.19QANALGRR86 pKa = 11.84RR87 pKa = 11.84PEE89 pKa = 4.03EE90 pKa = 3.45RR91 pKa = 11.84TLPIEE96 pKa = 4.14RR97 pKa = 11.84QRR99 pKa = 11.84AIANLVYY106 pKa = 10.22SKK108 pKa = 11.17RR109 pKa = 11.84MGNNAPGDD117 pKa = 3.23GWLYY121 pKa = 10.48RR122 pKa = 11.84GRR124 pKa = 11.84GLIQITGLNNYY135 pKa = 8.63RR136 pKa = 11.84DD137 pKa = 4.19CGNGLKK143 pKa = 10.05VDD145 pKa = 4.38LVKK148 pKa = 10.71QPQLLAEE155 pKa = 4.43DD156 pKa = 4.76VYY158 pKa = 11.38AARR161 pKa = 11.84SAAWFFVTKK170 pKa = 10.57GCLNHH175 pKa = 6.63SGDD178 pKa = 3.92LMRR181 pKa = 11.84VTKK184 pKa = 10.41IINGGTNGLEE194 pKa = 3.96DD195 pKa = 3.05RR196 pKa = 11.84RR197 pKa = 11.84ARR199 pKa = 11.84FGQAKK204 pKa = 7.84TVLVV208 pKa = 3.9

Molecular weight: 23.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

12440

42

1149

210.8

23.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.992 ± 0.543

1.182 ± 0.143

5.772 ± 0.269

6.222 ± 0.32

3.304 ± 0.195

7.613 ± 0.339

1.72 ± 0.232

5.498 ± 0.242

5.225 ± 0.308

7.958 ± 0.33

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.159

4.566 ± 0.224

4.188 ± 0.309

4.542 ± 0.238

5.82 ± 0.257

6.809 ± 0.357

5.892 ± 0.34

6.752 ± 0.243

1.833 ± 0.129

2.556 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski