Firmicutes bacterium CAG:582

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1293 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6GRD9|R6GRD9_9FIRM 30S ribosomal protein S6 OS=Firmicutes bacterium CAG:582 OX=1262997 GN=rpsF PE=3 SV=1
MM1 pKa = 7.72KK2 pKa = 10.04IIEE5 pKa = 4.4YY6 pKa = 9.53EE7 pKa = 3.99DD8 pKa = 4.26KK9 pKa = 11.07YY10 pKa = 11.52LEE12 pKa = 4.35DD13 pKa = 3.62VKK15 pKa = 11.17EE16 pKa = 4.17LLVQLEE22 pKa = 4.47EE23 pKa = 4.6YY24 pKa = 10.09IVSIDD29 pKa = 4.55KK30 pKa = 10.93DD31 pKa = 3.8CLDD34 pKa = 4.18QVHH37 pKa = 6.37SDD39 pKa = 3.47YY40 pKa = 10.72RR41 pKa = 11.84EE42 pKa = 3.67KK43 pKa = 10.29MACIDD48 pKa = 4.26LKK50 pKa = 11.17DD51 pKa = 3.82VNDD54 pKa = 4.11NNGKK58 pKa = 10.42CYY60 pKa = 10.18LALEE64 pKa = 4.42NDD66 pKa = 4.21NILGLIMGYY75 pKa = 9.82IPMHH79 pKa = 7.18DD80 pKa = 3.53EE81 pKa = 4.78FDD83 pKa = 3.97YY84 pKa = 11.55LDD86 pKa = 3.66YY87 pKa = 11.58

Molecular weight: 10.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6HR47|R6HR47_9FIRM 30S ribosomal protein S15 OS=Firmicutes bacterium CAG:582 OX=1262997 GN=rpsO PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.47KK12 pKa = 10.26SKK14 pKa = 9.93KK15 pKa = 9.34HH16 pKa = 5.08GFFARR21 pKa = 11.84KK22 pKa = 8.75NSKK25 pKa = 9.3ILKK28 pKa = 6.75TRR30 pKa = 11.84RR31 pKa = 11.84AKK33 pKa = 10.06GRR35 pKa = 11.84KK36 pKa = 8.35ILSRR40 pKa = 3.77

Molecular weight: 4.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1293

0

1293

388265

29

2192

300.3

34.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.28 ± 0.058

1.189 ± 0.028

6.243 ± 0.068

7.277 ± 0.081

4.471 ± 0.059

5.208 ± 0.096

1.341 ± 0.025

9.837 ± 0.081

9.761 ± 0.069

9.035 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.363 ± 0.029

7.409 ± 0.071

2.251 ± 0.034

1.899 ± 0.027

3.046 ± 0.046

6.714 ± 0.065

5.554 ± 0.092

6.166 ± 0.056

0.507 ± 0.017

5.449 ± 0.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski