Amino acid dipepetide frequency for Streptomyces sp. CB01883

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.951AlaAla: 21.951 ± 0.173
1.101AlaCys: 1.101 ± 0.022
8.477AlaAsp: 8.477 ± 0.081
8.927AlaGlu: 8.927 ± 0.083
3.577AlaPhe: 3.577 ± 0.043
13.673AlaGly: 13.673 ± 0.101
3.069AlaHis: 3.069 ± 0.038
3.143AlaIle: 3.143 ± 0.047
2.801AlaLys: 2.801 ± 0.055
14.821AlaLeu: 14.821 ± 0.12
2.41AlaMet: 2.41 ± 0.034
1.879AlaAsn: 1.879 ± 0.031
7.227AlaPro: 7.227 ± 0.09
3.722AlaGln: 3.722 ± 0.043
10.852AlaArg: 10.852 ± 0.079
5.764AlaSer: 5.764 ± 0.05
6.979AlaThr: 6.979 ± 0.066
12.815AlaVal: 12.815 ± 0.096
1.892AlaTrp: 1.892 ± 0.029
2.823AlaTyr: 2.823 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
1.121CysAla: 1.121 ± 0.023
0.104CysCys: 0.104 ± 0.008
0.442CysAsp: 0.442 ± 0.014
0.393CysGlu: 0.393 ± 0.014
0.222CysPhe: 0.222 ± 0.011
0.963CysGly: 0.963 ± 0.021
0.198CysHis: 0.198 ± 0.009
0.159CysIle: 0.159 ± 0.008
0.11CysLys: 0.11 ± 0.006
0.799CysLeu: 0.799 ± 0.021
0.118CysMet: 0.118 ± 0.006
0.132CysAsn: 0.132 ± 0.008
0.501CysPro: 0.501 ± 0.016
0.163CysGln: 0.163 ± 0.008
0.613CysArg: 0.613 ± 0.018
0.402CysSer: 0.402 ± 0.012
0.524CysThr: 0.524 ± 0.014
0.691CysVal: 0.691 ± 0.017
0.127CysTrp: 0.127 ± 0.008
0.148CysTyr: 0.148 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.599AspAla: 7.599 ± 0.07
0.42AspCys: 0.42 ± 0.013
3.664AspAsp: 3.664 ± 0.044
3.735AspGlu: 3.735 ± 0.047
1.644AspPhe: 1.644 ± 0.028
6.622AspGly: 6.622 ± 0.073
1.453AspHis: 1.453 ± 0.026
1.79AspIle: 1.79 ± 0.03
1.184AspLys: 1.184 ± 0.026
6.317AspLeu: 6.317 ± 0.06
0.815AspMet: 0.815 ± 0.02
0.933AspAsn: 0.933 ± 0.022
4.536AspPro: 4.536 ± 0.055
1.473AspGln: 1.473 ± 0.027
5.053AspArg: 5.053 ± 0.054
2.448AspSer: 2.448 ± 0.036
3.328AspThr: 3.328 ± 0.043
4.819AspVal: 4.819 ± 0.051
1.062AspTrp: 1.062 ± 0.025
1.106AspTyr: 1.106 ± 0.023
0.0AspXaa: 0.0 ± 0.0
Glu
7.361GluAla: 7.361 ± 0.083
0.348GluCys: 0.348 ± 0.014
2.899GluAsp: 2.899 ± 0.038
3.567GluGlu: 3.567 ± 0.054
1.427GluPhe: 1.427 ± 0.029
4.242GluGly: 4.242 ± 0.049
1.534GluHis: 1.534 ± 0.028
2.14GluIle: 2.14 ± 0.039
1.376GluLys: 1.376 ± 0.028
6.666GluLeu: 6.666 ± 0.061
0.848GluMet: 0.848 ± 0.02
0.992GluAsn: 0.992 ± 0.021
3.375GluPro: 3.375 ± 0.045
2.171GluGln: 2.171 ± 0.032
5.56GluArg: 5.56 ± 0.056
2.443GluSer: 2.443 ± 0.037
2.833GluThr: 2.833 ± 0.036
4.403GluVal: 4.403 ± 0.053
0.758GluTrp: 0.758 ± 0.022
1.066GluTyr: 1.066 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.715PheAla: 3.715 ± 0.039
0.271PheCys: 0.271 ± 0.012
1.927PheAsp: 1.927 ± 0.032
1.414PheGlu: 1.414 ± 0.025
0.896PhePhe: 0.896 ± 0.024
3.054PheGly: 3.054 ± 0.038
0.62PheHis: 0.62 ± 0.015
0.634PheIle: 0.634 ± 0.018
0.509PheLys: 0.509 ± 0.017
2.644PheLeu: 2.644 ± 0.038
0.385PheMet: 0.385 ± 0.013
0.533PheAsn: 0.533 ± 0.017
1.387PhePro: 1.387 ± 0.025
0.658PheGln: 0.658 ± 0.018
1.901PheArg: 1.901 ± 0.027
1.394PheSer: 1.394 ± 0.026
2.033PheThr: 2.033 ± 0.034
2.241PheVal: 2.241 ± 0.036
0.416PheTrp: 0.416 ± 0.014
0.575PheTyr: 0.575 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
11.363GlyAla: 11.363 ± 0.082
0.853GlyCys: 0.853 ± 0.02
5.336GlyAsp: 5.336 ± 0.059
4.999GlyGlu: 4.999 ± 0.053
2.897GlyPhe: 2.897 ± 0.038
8.971GlyGly: 8.971 ± 0.084
2.548GlyHis: 2.548 ± 0.033
3.305GlyIle: 3.305 ± 0.036
2.377GlyLys: 2.377 ± 0.037
9.58GlyLeu: 9.58 ± 0.084
1.969GlyMet: 1.969 ± 0.028
1.68GlyAsn: 1.68 ± 0.033
5.32GlyPro: 5.32 ± 0.064
2.593GlyGln: 2.593 ± 0.037
8.352GlyArg: 8.352 ± 0.071
5.248GlySer: 5.248 ± 0.055
6.717GlyThr: 6.717 ± 0.06
7.638GlyVal: 7.638 ± 0.064
1.721GlyTrp: 1.721 ± 0.031
2.301GlyTyr: 2.301 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
2.83HisAla: 2.83 ± 0.039
0.213HisCys: 0.213 ± 0.01
1.394HisAsp: 1.394 ± 0.025
1.258HisGlu: 1.258 ± 0.025
0.675HisPhe: 0.675 ± 0.017
2.617HisGly: 2.617 ± 0.035
0.713HisHis: 0.713 ± 0.02
0.638HisIle: 0.638 ± 0.015
0.36HisLys: 0.36 ± 0.012
2.51HisLeu: 2.51 ± 0.039
0.356HisMet: 0.356 ± 0.012
0.335HisAsn: 0.335 ± 0.011
1.932HisPro: 1.932 ± 0.033
0.621HisGln: 0.621 ± 0.019
2.222HisArg: 2.222 ± 0.034
1.024HisSer: 1.024 ± 0.019
1.435HisThr: 1.435 ± 0.027
1.78HisVal: 1.78 ± 0.027
0.385HisTrp: 0.385 ± 0.013
0.501HisTyr: 0.501 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.523IleAla: 4.523 ± 0.049
0.269IleCys: 0.269 ± 0.011
2.114IleAsp: 2.114 ± 0.034
1.809IleGlu: 1.809 ± 0.033
0.622IlePhe: 0.622 ± 0.018
3.419IleGly: 3.419 ± 0.042
0.586IleHis: 0.586 ± 0.015
0.78IleIle: 0.78 ± 0.021
0.704IleLys: 0.704 ± 0.02
2.297IleLeu: 2.297 ± 0.032
0.409IleMet: 0.409 ± 0.014
0.638IleAsn: 0.638 ± 0.019
1.626IlePro: 1.626 ± 0.027
0.674IleGln: 0.674 ± 0.017
2.089IleArg: 2.089 ± 0.033
1.489IleSer: 1.489 ± 0.027
2.087IleThr: 2.087 ± 0.032
2.598IleVal: 2.598 ± 0.039
0.348IleTrp: 0.348 ± 0.013
0.489IleTyr: 0.489 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
2.939LysAla: 2.939 ± 0.045
0.111LysCys: 0.111 ± 0.007
1.362LysAsp: 1.362 ± 0.031
1.112LysGlu: 1.112 ± 0.027
0.448LysPhe: 0.448 ± 0.017
1.874LysGly: 1.874 ± 0.035
0.425LysHis: 0.425 ± 0.014
0.839LysIle: 0.839 ± 0.024
0.896LysLys: 0.896 ± 0.03
1.926LysLeu: 1.926 ± 0.036
0.365LysMet: 0.365 ± 0.013
0.517LysAsn: 0.517 ± 0.018
1.29LysPro: 1.29 ± 0.026
0.692LysGln: 0.692 ± 0.02
1.39LysArg: 1.39 ± 0.025
1.097LysSer: 1.097 ± 0.023
1.229LysThr: 1.229 ± 0.03
1.927LysVal: 1.927 ± 0.038
0.274LysTrp: 0.274 ± 0.012
0.451LysTyr: 0.451 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
15.458LeuAla: 15.458 ± 0.115
0.867LeuCys: 0.867 ± 0.021
6.752LeuAsp: 6.752 ± 0.059
4.693LeuGlu: 4.693 ± 0.057
2.586LeuPhe: 2.586 ± 0.039
9.467LeuGly: 9.467 ± 0.068
2.389LeuHis: 2.389 ± 0.036
3.161LeuIle: 3.161 ± 0.04
2.054LeuLys: 2.054 ± 0.036
11.658LeuLeu: 11.658 ± 0.104
1.63LeuMet: 1.63 ± 0.033
1.614LeuAsn: 1.614 ± 0.028
6.626LeuPro: 6.626 ± 0.063
2.212LeuGln: 2.212 ± 0.037
8.874LeuArg: 8.874 ± 0.079
5.196LeuSer: 5.196 ± 0.048
6.973LeuThr: 6.973 ± 0.06
8.925LeuVal: 8.925 ± 0.074
1.348LeuTrp: 1.348 ± 0.025
1.947LeuTyr: 1.947 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
2.194MetAla: 2.194 ± 0.032
0.135MetCys: 0.135 ± 0.007
0.876MetAsp: 0.876 ± 0.02
0.716MetGlu: 0.716 ± 0.018
0.461MetPhe: 0.461 ± 0.016
1.276MetGly: 1.276 ± 0.024
0.37MetHis: 0.37 ± 0.014
0.608MetIle: 0.608 ± 0.016
0.389MetLys: 0.389 ± 0.015
1.587MetLeu: 1.587 ± 0.03
0.283MetMet: 0.283 ± 0.012
0.417MetAsn: 0.417 ± 0.015
1.078MetPro: 1.078 ± 0.021
0.435MetGln: 0.435 ± 0.015
1.443MetArg: 1.443 ± 0.026
1.282MetSer: 1.282 ± 0.023
1.557MetThr: 1.557 ± 0.025
1.222MetVal: 1.222 ± 0.028
0.21MetTrp: 0.21 ± 0.009
0.326MetTyr: 0.326 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.097AsnAla: 2.097 ± 0.032
0.161AsnCys: 0.161 ± 0.008
0.885AsnAsp: 0.885 ± 0.018
0.734AsnGlu: 0.734 ± 0.019
0.465AsnPhe: 0.465 ± 0.013
1.804AsnGly: 1.804 ± 0.031
0.397AsnHis: 0.397 ± 0.014
0.607AsnIle: 0.607 ± 0.019
0.394AsnLys: 0.394 ± 0.016
1.587AsnLeu: 1.587 ± 0.032
0.293AsnMet: 0.293 ± 0.012
0.376AsnAsn: 0.376 ± 0.017
1.305AsnPro: 1.305 ± 0.023
0.473AsnGln: 0.473 ± 0.015
1.24AsnArg: 1.24 ± 0.023
0.889AsnSer: 0.889 ± 0.021
1.059AsnThr: 1.059 ± 0.027
1.386AsnVal: 1.386 ± 0.028
0.28AsnTrp: 0.28 ± 0.011
0.384AsnTyr: 0.384 ± 0.013
0.0AsnXaa: 0.0 ± 0.0
Pro
9.237ProAla: 9.237 ± 0.095
0.343ProCys: 0.343 ± 0.011
4.515ProAsp: 4.515 ± 0.054
4.332ProGlu: 4.332 ± 0.049
1.535ProPhe: 1.535 ± 0.031
6.785ProGly: 6.785 ± 0.061
1.478ProHis: 1.478 ± 0.027
1.105ProIle: 1.105 ± 0.021
1.134ProLys: 1.134 ± 0.023
5.488ProLeu: 5.488 ± 0.062
0.946ProMet: 0.946 ± 0.022
0.842ProAsn: 0.842 ± 0.021
3.561ProPro: 3.561 ± 0.053
1.646ProGln: 1.646 ± 0.034
4.092ProArg: 4.092 ± 0.047
3.039ProSer: 3.039 ± 0.04
3.025ProThr: 3.025 ± 0.038
5.608ProVal: 5.608 ± 0.051
0.884ProTrp: 0.884 ± 0.02
1.488ProTyr: 1.488 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.65GlnAla: 3.65 ± 0.041
0.184GlnCys: 0.184 ± 0.008
1.376GlnAsp: 1.376 ± 0.026
1.427GlnGlu: 1.427 ± 0.028
0.651GlnPhe: 0.651 ± 0.018
2.299GlnGly: 2.299 ± 0.034
0.661GlnHis: 0.661 ± 0.017
1.024GlnIle: 1.024 ± 0.021
0.612GlnLys: 0.612 ± 0.018
2.949GlnLeu: 2.949 ± 0.032
0.464GlnMet: 0.464 ± 0.015
0.469GlnAsn: 0.469 ± 0.016
1.622GlnPro: 1.622 ± 0.039
1.224GlnGln: 1.224 ± 0.029
2.344GlnArg: 2.344 ± 0.033
1.226GlnSer: 1.226 ± 0.024
1.312GlnThr: 1.312 ± 0.025
2.344GlnVal: 2.344 ± 0.03
0.456GlnTrp: 0.456 ± 0.013
0.583GlnTyr: 0.583 ± 0.015
0.0GlnXaa: 0.0 ± 0.0
Arg
10.466ArgAla: 10.466 ± 0.103
0.602ArgCys: 0.602 ± 0.016
4.305ArgAsp: 4.305 ± 0.044
4.851ArgGlu: 4.851 ± 0.049
2.36ArgPhe: 2.36 ± 0.034
5.954ArgGly: 5.954 ± 0.049
2.26ArgHis: 2.26 ± 0.032
3.188ArgIle: 3.188 ± 0.039
1.598ArgLys: 1.598 ± 0.028
9.326ArgLeu: 9.326 ± 0.08
1.769ArgMet: 1.769 ± 0.027
1.306ArgAsn: 1.306 ± 0.026
5.225ArgPro: 5.225 ± 0.056
2.303ArgGln: 2.303 ± 0.03
8.083ArgArg: 8.083 ± 0.083
3.965ArgSer: 3.965 ± 0.037
5.625ArgThr: 5.625 ± 0.058
6.08ArgVal: 6.08 ± 0.062
1.367ArgTrp: 1.367 ± 0.023
1.853ArgTyr: 1.853 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
6.741SerAla: 6.741 ± 0.065
0.409SerCys: 0.409 ± 0.014
2.519SerAsp: 2.519 ± 0.035
2.234SerGlu: 2.234 ± 0.034
1.51SerPhe: 1.51 ± 0.027
5.831SerGly: 5.831 ± 0.051
0.983SerHis: 0.983 ± 0.02
1.254SerIle: 1.254 ± 0.027
0.992SerLys: 0.992 ± 0.025
4.831SerLeu: 4.831 ± 0.052
1.004SerMet: 1.004 ± 0.02
0.812SerAsn: 0.812 ± 0.018
3.013SerPro: 3.013 ± 0.041
1.162SerGln: 1.162 ± 0.025
3.606SerArg: 3.606 ± 0.043
2.674SerSer: 2.674 ± 0.043
2.872SerThr: 2.872 ± 0.039
4.159SerVal: 4.159 ± 0.046
0.832SerTrp: 0.832 ± 0.018
1.233SerTyr: 1.233 ± 0.031
0.0SerXaa: 0.0 ± 0.0
Thr
9.252ThrAla: 9.252 ± 0.08
0.415ThrCys: 0.415 ± 0.012
3.738ThrAsp: 3.738 ± 0.045
3.259ThrGlu: 3.259 ± 0.042
1.593ThrPhe: 1.593 ± 0.032
7.009ThrGly: 7.009 ± 0.055
1.245ThrHis: 1.245 ± 0.023
1.54ThrIle: 1.54 ± 0.026
1.147ThrLys: 1.147 ± 0.025
5.729ThrLeu: 5.729 ± 0.056
0.908ThrMet: 0.908 ± 0.021
0.926ThrAsn: 0.926 ± 0.02
4.078ThrPro: 4.078 ± 0.048
1.325ThrGln: 1.325 ± 0.023
4.022ThrArg: 4.022 ± 0.046
3.002ThrSer: 3.002 ± 0.041
3.802ThrThr: 3.802 ± 0.055
6.237ThrVal: 6.237 ± 0.054
0.93ThrTrp: 0.93 ± 0.019
1.318ThrTyr: 1.318 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
10.879ValAla: 10.879 ± 0.091
0.787ValCys: 0.787 ± 0.018
4.981ValAsp: 4.981 ± 0.057
4.583ValGlu: 4.583 ± 0.054
2.467ValPhe: 2.467 ± 0.035
6.485ValGly: 6.485 ± 0.055
2.084ValHis: 2.084 ± 0.031
2.644ValIle: 2.644 ± 0.04
1.755ValLys: 1.755 ± 0.031
9.819ValLeu: 9.819 ± 0.076
1.363ValMet: 1.363 ± 0.026
1.636ValAsn: 1.636 ± 0.028
5.537ValPro: 5.537 ± 0.048
2.122ValGln: 2.122 ± 0.029
7.528ValArg: 7.528 ± 0.069
4.261ValSer: 4.261 ± 0.046
5.799ValThr: 5.799 ± 0.056
8.177ValVal: 8.177 ± 0.077
1.173ValTrp: 1.173 ± 0.022
1.646ValTyr: 1.646 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.682TrpAla: 1.682 ± 0.031
0.162TrpCys: 0.162 ± 0.01
0.86TrpAsp: 0.86 ± 0.026
0.697TrpGlu: 0.697 ± 0.016
0.504TrpPhe: 0.504 ± 0.014
1.035TrpGly: 1.035 ± 0.021
0.39TrpHis: 0.39 ± 0.013
0.539TrpIle: 0.539 ± 0.014
0.371TrpLys: 0.371 ± 0.012
1.791TrpLeu: 1.791 ± 0.03
0.277TrpMet: 0.277 ± 0.01
0.383TrpAsn: 0.383 ± 0.013
0.785TrpPro: 0.785 ± 0.019
0.631TrpGln: 0.631 ± 0.017
1.376TrpArg: 1.376 ± 0.024
0.912TrpSer: 0.912 ± 0.019
1.042TrpThr: 1.042 ± 0.025
0.98TrpVal: 0.98 ± 0.018
0.359TrpTrp: 0.359 ± 0.013
0.386TrpTyr: 0.386 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.842TyrAla: 2.842 ± 0.039
0.177TyrCys: 0.177 ± 0.008
1.582TyrAsp: 1.582 ± 0.03
1.269TyrGlu: 1.269 ± 0.022
0.666TyrPhe: 0.666 ± 0.02
2.389TyrGly: 2.389 ± 0.036
0.395TyrHis: 0.395 ± 0.016
0.471TyrIle: 0.471 ± 0.016
0.401TyrLys: 0.401 ± 0.015
2.103TyrLeu: 2.103 ± 0.033
0.252TyrMet: 0.252 ± 0.011
0.396TyrAsn: 0.396 ± 0.013
1.057TyrPro: 1.057 ± 0.024
0.58TyrGln: 0.58 ± 0.014
1.844TyrArg: 1.844 ± 0.029
0.917TyrSer: 0.917 ± 0.02
1.243TyrThr: 1.243 ± 0.027
1.696TyrVal: 1.696 ± 0.029
0.343TyrTrp: 0.343 ± 0.013
0.438TyrTyr: 0.438 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7450 proteins (2405307 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski