Amino acid dipepetide frequency for Saccharopolyspora antimicrobica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.197AlaAla: 20.197 ± 0.147	1.011AlaCys: 1.011 ± 0.022	8.231AlaAsp: 8.231 ± 0.073	10.453AlaGlu: 10.453 ± 0.094	3.555AlaPhe: 3.555 ± 0.04	12.29AlaGly: 12.29 ± 0.085	2.685AlaHis: 2.685 ± 0.04	4.441AlaIle: 4.441 ± 0.05	2.731AlaLys: 2.731 ± 0.038	13.904AlaLeu: 13.904 ± 0.099	2.709AlaMet: 2.709 ± 0.035	2.192AlaAsn: 2.192 ± 0.035	6.28AlaPro: 6.28 ± 0.064	3.998AlaGln: 3.998 ± 0.042	9.3AlaArg: 9.3 ± 0.073	5.49AlaSer: 5.49 ± 0.052	6.539AlaThr: 6.539 ± 0.057	12.132AlaVal: 12.132 ± 0.091	1.72AlaTrp: 1.72 ± 0.026	2.132AlaTyr: 2.132 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	1.112CysAla: 1.112 ± 0.021	0.106CysCys: 0.106 ± 0.007	0.455CysAsp: 0.455 ± 0.013	0.465CysGlu: 0.465 ± 0.015	0.246CysPhe: 0.246 ± 0.011	0.966CysGly: 0.966 ± 0.023	0.198CysHis: 0.198 ± 0.009	0.194CysIle: 0.194 ± 0.01	0.099CysLys: 0.099 ± 0.007	0.737CysLeu: 0.737 ± 0.019	0.133CysMet: 0.133 ± 0.007	0.136CysAsn: 0.136 ± 0.008	0.47CysPro: 0.47 ± 0.015	0.211CysGln: 0.211 ± 0.009	0.591CysArg: 0.591 ± 0.014	0.495CysSer: 0.495 ± 0.015	0.51CysThr: 0.51 ± 0.015	0.627CysVal: 0.627 ± 0.019	0.138CysTrp: 0.138 ± 0.007	0.197CysTyr: 0.197 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.142AspAla: 7.142 ± 0.063	0.437AspCys: 0.437 ± 0.015	3.633AspAsp: 3.633 ± 0.046	4.271AspGlu: 4.271 ± 0.051	1.712AspPhe: 1.712 ± 0.029	5.809AspGly: 5.809 ± 0.064	1.373AspHis: 1.373 ± 0.024	1.83AspIle: 1.83 ± 0.026	0.952AspLys: 0.952 ± 0.024	6.325AspLeu: 6.325 ± 0.059	0.807AspMet: 0.807 ± 0.018	1.006AspAsn: 1.006 ± 0.024	4.346AspPro: 4.346 ± 0.045	1.899AspGln: 1.899 ± 0.032	4.919AspArg: 4.919 ± 0.047	2.646AspSer: 2.646 ± 0.041	2.591AspThr: 2.591 ± 0.033	5.211AspVal: 5.211 ± 0.052	0.951AspTrp: 0.951 ± 0.021	1.19AspTyr: 1.19 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	7.002GluAla: 7.002 ± 0.068	0.397GluCys: 0.397 ± 0.012	2.701GluAsp: 2.701 ± 0.04	3.24GluGlu: 3.24 ± 0.045	1.954GluPhe: 1.954 ± 0.028	3.518GluGly: 3.518 ± 0.042	2.077GluHis: 2.077 ± 0.03	2.751GluIle: 2.751 ± 0.034	1.265GluLys: 1.265 ± 0.025	8.376GluLeu: 8.376 ± 0.07	1.008GluMet: 1.008 ± 0.022	1.098GluAsn: 1.098 ± 0.021	3.885GluPro: 3.885 ± 0.053	3.08GluGln: 3.08 ± 0.04	5.596GluArg: 5.596 ± 0.059	2.93GluSer: 2.93 ± 0.043	2.731GluThr: 2.731 ± 0.039	5.373GluVal: 5.373 ± 0.052	0.876GluTrp: 0.876 ± 0.019	1.015GluTyr: 1.015 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	4.201PheAla: 4.201 ± 0.045	0.284PheCys: 0.284 ± 0.011	2.177PheAsp: 2.177 ± 0.031	1.577PheGlu: 1.577 ± 0.028	0.904PhePhe: 0.904 ± 0.021	3.473PheGly: 3.473 ± 0.042	0.662PheHis: 0.662 ± 0.017	0.808PheIle: 0.808 ± 0.022	0.426PheLys: 0.426 ± 0.014	2.708PheLeu: 2.708 ± 0.04	0.382PheMet: 0.382 ± 0.012	0.62PheAsn: 0.62 ± 0.015	1.444PhePro: 1.444 ± 0.029	0.75PheGln: 0.75 ± 0.019	1.96PheArg: 1.96 ± 0.03	1.59PheSer: 1.59 ± 0.031	1.942PheThr: 1.942 ± 0.029	2.353PheVal: 2.353 ± 0.031	0.445PheTrp: 0.445 ± 0.015	0.594PheTyr: 0.594 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	10.327GlyAla: 10.327 ± 0.074	0.838GlyCys: 0.838 ± 0.019	4.698GlyAsp: 4.698 ± 0.048	5.537GlyGlu: 5.537 ± 0.053	3.065GlyPhe: 3.065 ± 0.04	8.23GlyGly: 8.23 ± 0.11	2.057GlyHis: 2.057 ± 0.029	3.83GlyIle: 3.83 ± 0.036	2.232GlyLys: 2.232 ± 0.032	9.311GlyLeu: 9.311 ± 0.068	2.147GlyMet: 2.147 ± 0.03	1.803GlyAsn: 1.803 ± 0.03	4.606GlyPro: 4.606 ± 0.05	2.831GlyGln: 2.831 ± 0.042	6.902GlyArg: 6.902 ± 0.053	5.301GlySer: 5.301 ± 0.061	5.38GlyThr: 5.38 ± 0.05	7.636GlyVal: 7.636 ± 0.061	1.756GlyTrp: 1.756 ± 0.029	2.203GlyTyr: 2.203 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	2.588HisAla: 2.588 ± 0.033	0.245HisCys: 0.245 ± 0.01	1.415HisAsp: 1.415 ± 0.025	1.284HisGlu: 1.284 ± 0.025	0.688HisPhe: 0.688 ± 0.018	2.285HisGly: 2.285 ± 0.038	0.681HisHis: 0.681 ± 0.017	0.638HisIle: 0.638 ± 0.016	0.284HisLys: 0.284 ± 0.012	2.44HisLeu: 2.44 ± 0.037	0.291HisMet: 0.291 ± 0.012	0.415HisAsn: 0.415 ± 0.013	1.715HisPro: 1.715 ± 0.027	0.708HisGln: 0.708 ± 0.018	2.235HisArg: 2.235 ± 0.033	1.041HisSer: 1.041 ± 0.019	1.086HisThr: 1.086 ± 0.022	1.824HisVal: 1.824 ± 0.028	0.392HisTrp: 0.392 ± 0.015	0.53HisTyr: 0.53 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	5.839IleAla: 5.839 ± 0.054	0.33IleCys: 0.33 ± 0.012	2.571IleAsp: 2.571 ± 0.035	2.182IleGlu: 2.182 ± 0.029	0.79IlePhe: 0.79 ± 0.019	4.33IleGly: 4.33 ± 0.048	0.58IleHis: 0.58 ± 0.015	1.045IleIle: 1.045 ± 0.026	0.709IleLys: 0.709 ± 0.017	2.294IleLeu: 2.294 ± 0.034	0.487IleMet: 0.487 ± 0.012	0.877IleAsn: 0.877 ± 0.02	1.993IlePro: 1.993 ± 0.029	0.713IleGln: 0.713 ± 0.015	2.568IleArg: 2.568 ± 0.03	2.12IleSer: 2.12 ± 0.029	2.634IleThr: 2.634 ± 0.035	2.949IleVal: 2.949 ± 0.036	0.42IleTrp: 0.42 ± 0.013	0.609IleTyr: 0.609 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	2.366LysAla: 2.366 ± 0.039	0.109LysCys: 0.109 ± 0.008	0.936LysAsp: 0.936 ± 0.023	0.928LysGlu: 0.928 ± 0.02	0.499LysPhe: 0.499 ± 0.015	1.265LysGly: 1.265 ± 0.027	0.508LysHis: 0.508 ± 0.014	0.841LysIle: 0.841 ± 0.019	0.573LysLys: 0.573 ± 0.019	2.124LysLeu: 2.124 ± 0.035	0.355LysMet: 0.355 ± 0.012	0.408LysAsn: 0.408 ± 0.014	1.378LysPro: 1.378 ± 0.029	0.775LysGln: 0.775 ± 0.019	1.52LysArg: 1.52 ± 0.027	1.066LysSer: 1.066 ± 0.022	1.077LysThr: 1.077 ± 0.022	1.684LysVal: 1.684 ± 0.029	0.29LysTrp: 0.29 ± 0.012	0.377LysTyr: 0.377 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	15.499LeuAla: 15.499 ± 0.107	0.865LeuCys: 0.865 ± 0.016	6.786LeuAsp: 6.786 ± 0.064	4.821LeuGlu: 4.821 ± 0.059	2.806LeuPhe: 2.806 ± 0.036	9.537LeuGly: 9.537 ± 0.087	2.364LeuHis: 2.364 ± 0.033	3.843LeuIle: 3.843 ± 0.046	1.576LeuLys: 1.576 ± 0.03	11.693LeuLeu: 11.693 ± 0.114	1.515LeuMet: 1.515 ± 0.025	1.835LeuAsn: 1.835 ± 0.027	6.487LeuPro: 6.487 ± 0.059	2.549LeuGln: 2.549 ± 0.033	9.095LeuArg: 9.095 ± 0.077	5.669LeuSer: 5.669 ± 0.051	6.13LeuThr: 6.13 ± 0.057	9.748LeuVal: 9.748 ± 0.081	1.285LeuTrp: 1.285 ± 0.027	1.511LeuTyr: 1.511 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	2.245MetAla: 2.245 ± 0.032	0.153MetCys: 0.153 ± 0.009	0.777MetAsp: 0.777 ± 0.021	0.622MetGlu: 0.622 ± 0.017	0.56MetPhe: 0.56 ± 0.016	1.271MetGly: 1.271 ± 0.026	0.408MetHis: 0.408 ± 0.013	0.75MetIle: 0.75 ± 0.02	0.33MetLys: 0.33 ± 0.012	1.972MetLeu: 1.972 ± 0.026	0.329MetMet: 0.329 ± 0.012	0.44MetAsn: 0.44 ± 0.013	1.162MetPro: 1.162 ± 0.022	0.541MetGln: 0.541 ± 0.014	1.572MetArg: 1.572 ± 0.025	1.423MetSer: 1.423 ± 0.024	1.553MetThr: 1.553 ± 0.03	1.383MetVal: 1.383 ± 0.026	0.215MetTrp: 0.215 ± 0.009	0.273MetTyr: 0.273 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.458AsnAla: 2.458 ± 0.037	0.177AsnCys: 0.177 ± 0.009	0.976AsnAsp: 0.976 ± 0.022	0.875AsnGlu: 0.875 ± 0.018	0.561AsnPhe: 0.561 ± 0.017	1.863AsnGly: 1.863 ± 0.035	0.403AsnHis: 0.403 ± 0.012	0.692AsnIle: 0.692 ± 0.019	0.358AsnLys: 0.358 ± 0.013	1.874AsnLeu: 1.874 ± 0.029	0.314AsnMet: 0.314 ± 0.012	0.46AsnAsn: 0.46 ± 0.017	1.611AsnPro: 1.611 ± 0.029	0.597AsnGln: 0.597 ± 0.014	1.505AsnArg: 1.505 ± 0.026	1.036AsnSer: 1.036 ± 0.023	1.127AsnThr: 1.127 ± 0.026	1.444AsnVal: 1.444 ± 0.028	0.32AsnTrp: 0.32 ± 0.011	0.429AsnTyr: 0.429 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	7.684ProAla: 7.684 ± 0.07	0.354ProCys: 0.354 ± 0.012	4.419ProAsp: 4.419 ± 0.047	4.782ProGlu: 4.782 ± 0.05	1.561ProPhe: 1.561 ± 0.028	6.147ProGly: 6.147 ± 0.064	1.273ProHis: 1.273 ± 0.027	1.653ProIle: 1.653 ± 0.026	1.205ProLys: 1.205 ± 0.024	5.165ProLeu: 5.165 ± 0.049	1.071ProMet: 1.071 ± 0.02	1.177ProAsn: 1.177 ± 0.023	3.561ProPro: 3.561 ± 0.076	1.952ProGln: 1.952 ± 0.033	3.649ProArg: 3.649 ± 0.045	2.902ProSer: 2.902 ± 0.041	3.086ProThr: 3.086 ± 0.04	5.477ProVal: 5.477 ± 0.047	0.91ProTrp: 0.91 ± 0.022	0.989ProTyr: 0.989 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	3.84GlnAla: 3.84 ± 0.046	0.201GlnCys: 0.201 ± 0.009	1.433GlnAsp: 1.433 ± 0.025	1.54GlnGlu: 1.54 ± 0.028	0.845GlnPhe: 0.845 ± 0.019	2.049GlnGly: 2.049 ± 0.035	0.878GlnHis: 0.878 ± 0.018	1.269GlnIle: 1.269 ± 0.026	0.526GlnLys: 0.526 ± 0.015	3.861GlnLeu: 3.861 ± 0.044	0.505GlnMet: 0.505 ± 0.015	0.597GlnAsn: 0.597 ± 0.017	2.061GlnPro: 2.061 ± 0.037	1.7GlnGln: 1.7 ± 0.037	3.253GlnArg: 3.253 ± 0.042	1.285GlnSer: 1.285 ± 0.025	1.257GlnThr: 1.257 ± 0.024	2.948GlnVal: 2.948 ± 0.033	0.541GlnTrp: 0.541 ± 0.013	0.585GlnTyr: 0.585 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	9.596ArgAla: 9.596 ± 0.08	0.668ArgCys: 0.668 ± 0.019	4.294ArgAsp: 4.294 ± 0.046	5.172ArgGlu: 5.172 ± 0.046	2.543ArgPhe: 2.543 ± 0.032	5.807ArgGly: 5.807 ± 0.061	1.804ArgHis: 1.804 ± 0.032	3.548ArgIle: 3.548 ± 0.04	1.821ArgLys: 1.821 ± 0.031	8.292ArgLeu: 8.292 ± 0.079	1.971ArgMet: 1.971 ± 0.029	1.617ArgAsn: 1.617 ± 0.029	4.463ArgPro: 4.463 ± 0.052	2.466ArgGln: 2.466 ± 0.036	7.457ArgArg: 7.457 ± 0.064	4.446ArgSer: 4.446 ± 0.049	4.874ArgThr: 4.874 ± 0.05	5.696ArgVal: 5.696 ± 0.048	1.455ArgTrp: 1.455 ± 0.024	1.763ArgTyr: 1.763 ± 0.025	0.0ArgXaa: 0.0 ± 0.0
Ser	6.984SerAla: 6.984 ± 0.057	0.435SerCys: 0.435 ± 0.014	2.758SerAsp: 2.758 ± 0.038	2.659SerGlu: 2.659 ± 0.034	1.564SerPhe: 1.564 ± 0.025	6.007SerGly: 6.007 ± 0.06	0.933SerHis: 0.933 ± 0.019	1.794SerIle: 1.794 ± 0.034	1.019SerLys: 1.019 ± 0.024	4.779SerLeu: 4.779 ± 0.052	1.231SerMet: 1.231 ± 0.02	0.973SerAsn: 0.973 ± 0.022	3.049SerPro: 3.049 ± 0.038	1.308SerGln: 1.308 ± 0.026	3.785SerArg: 3.785 ± 0.044	3.055SerSer: 3.055 ± 0.045	3.284SerThr: 3.284 ± 0.039	4.291SerVal: 4.291 ± 0.043	1.001SerTrp: 1.001 ± 0.02	1.147SerTyr: 1.147 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	8.056ThrAla: 8.056 ± 0.059	0.436ThrCys: 0.436 ± 0.014	3.343ThrAsp: 3.343 ± 0.035	3.384ThrGlu: 3.384 ± 0.036	1.641ThrPhe: 1.641 ± 0.028	6.186ThrGly: 6.186 ± 0.061	1.032ThrHis: 1.032 ± 0.021	1.924ThrIle: 1.924 ± 0.028	1.069ThrLys: 1.069 ± 0.023	4.709ThrLeu: 4.709 ± 0.051	0.922ThrMet: 0.922 ± 0.02	1.053ThrAsn: 1.053 ± 0.023	3.509ThrPro: 3.509 ± 0.038	1.239ThrGln: 1.239 ± 0.026	3.687ThrArg: 3.687 ± 0.041	3.298ThrSer: 3.298 ± 0.04	3.79ThrThr: 3.79 ± 0.045	4.927ThrVal: 4.927 ± 0.053	0.867ThrTrp: 0.867 ± 0.021	1.096ThrTyr: 1.096 ± 0.023	0.0ThrXaa: 0.0 ± 0.0
Val	10.947ValAla: 10.947 ± 0.082	0.704ValCys: 0.704 ± 0.019	5.444ValAsp: 5.444 ± 0.053	5.366ValGlu: 5.366 ± 0.061	2.579ValPhe: 2.579 ± 0.037	6.866ValGly: 6.866 ± 0.058	2.085ValHis: 2.085 ± 0.033	3.16ValIle: 3.16 ± 0.041	1.335ValLys: 1.335 ± 0.026	10.962ValLeu: 10.962 ± 0.091	1.265ValMet: 1.265 ± 0.025	1.645ValAsn: 1.645 ± 0.028	5.091ValPro: 5.091 ± 0.043	2.492ValGln: 2.492 ± 0.032	6.97ValArg: 6.97 ± 0.053	4.307ValSer: 4.307 ± 0.045	4.681ValThr: 4.681 ± 0.045	8.932ValVal: 8.932 ± 0.081	1.032ValTrp: 1.032 ± 0.022	1.343ValTyr: 1.343 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.642TrpAla: 1.642 ± 0.027	0.149TrpCys: 0.149 ± 0.007	0.785TrpAsp: 0.785 ± 0.02	0.689TrpGlu: 0.689 ± 0.019	0.569TrpPhe: 0.569 ± 0.015	1.018TrpGly: 1.018 ± 0.021	0.373TrpHis: 0.373 ± 0.013	0.604TrpIle: 0.604 ± 0.017	0.289TrpLys: 0.289 ± 0.012	1.93TrpLeu: 1.93 ± 0.031	0.339TrpMet: 0.339 ± 0.015	0.375TrpAsn: 0.375 ± 0.013	0.845TrpPro: 0.845 ± 0.019	0.678TrpGln: 0.678 ± 0.019	1.376TrpArg: 1.376 ± 0.024	0.973TrpSer: 0.973 ± 0.021	0.93TrpThr: 0.93 ± 0.023	1.076TrpVal: 1.076 ± 0.022	0.372TrpTrp: 0.372 ± 0.013	0.303TrpTyr: 0.303 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.264TyrAla: 2.264 ± 0.03	0.185TyrCys: 0.185 ± 0.009	1.219TyrAsp: 1.219 ± 0.024	1.027TyrGlu: 1.027 ± 0.023	0.656TyrPhe: 0.656 ± 0.016	1.775TyrGly: 1.775 ± 0.025	0.411TyrHis: 0.411 ± 0.013	0.43TyrIle: 0.43 ± 0.015	0.276TyrLys: 0.276 ± 0.01	2.156TyrLeu: 2.156 ± 0.034	0.199TyrMet: 0.199 ± 0.009	0.352TyrAsn: 0.352 ± 0.012	1.064TyrPro: 1.064 ± 0.019	0.689TyrGln: 0.689 ± 0.017	1.813TyrArg: 1.813 ± 0.03	0.981TyrSer: 0.981 ± 0.021	0.962TyrThr: 0.962 ± 0.02	1.497TyrVal: 1.497 ± 0.025	0.33TyrTrp: 0.33 ± 0.013	0.453TyrTyr: 0.453 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 7655 proteins (2397594 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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