Mesorhizobium carbonis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4987 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A429Z023|A0A429Z023_9RHIZ Lipopolysaccharide biosynthesis protein OS=Mesorhizobium carbonis OX=2495581 GN=EJC49_07325 PE=4 SV=1
MM1 pKa = 7.58DD2 pKa = 3.33QRR4 pKa = 11.84RR5 pKa = 11.84TTRR8 pKa = 11.84ALLSGAALGTVFLALGMPAPAEE30 pKa = 4.1AACLADD36 pKa = 4.44GTTLTCTGDD45 pKa = 3.33LGAGVIVADD54 pKa = 4.4PIEE57 pKa = 4.17RR58 pKa = 11.84LEE60 pKa = 4.25INGVTAMIAPAEE72 pKa = 4.24GVSGIDD78 pKa = 3.69FQSSGVIAIVSDD90 pKa = 3.52TGAYY94 pKa = 9.62QILATDD100 pKa = 3.82ADD102 pKa = 4.99GIHH105 pKa = 6.78ADD107 pKa = 3.4SWNGSITIEE116 pKa = 3.95HH117 pKa = 6.45TGDD120 pKa = 2.9IRR122 pKa = 11.84SVGGYY127 pKa = 7.58GVRR130 pKa = 11.84TISLAGTSVTVDD142 pKa = 3.13GDD144 pKa = 3.71IEE146 pKa = 4.62ASLGGIVVNNDD157 pKa = 3.26DD158 pKa = 4.67AGTTTEE164 pKa = 4.22ISHH167 pKa = 6.51TGDD170 pKa = 2.93ILVTEE175 pKa = 4.79GAGISAVTSSTPLVVVNEE193 pKa = 4.05GSINASGDD201 pKa = 3.38GISAVNTSEE210 pKa = 4.23SGSVTVTQTGNVIAGDD226 pKa = 3.82RR227 pKa = 11.84GIHH230 pKa = 6.42ADD232 pKa = 3.34AAFGSVTLTSAGNISSVGDD251 pKa = 4.34GIQLLSTGSDD261 pKa = 3.06VTATHH266 pKa = 6.94TGNVTSTEE274 pKa = 3.91GRR276 pKa = 11.84GIFLDD281 pKa = 3.97APTAGASLSGSGTIQSYY298 pKa = 11.04GDD300 pKa = 3.76GIFAQSTGVAHH311 pKa = 5.9STSVTWTGNITSEE324 pKa = 3.93IGKK327 pKa = 10.21GIYY330 pKa = 10.01AYY332 pKa = 10.05AANGAVTVLSAGDD345 pKa = 3.61IVSSGDD351 pKa = 3.42SIFAMNVSTDD361 pKa = 3.11GTAEE365 pKa = 4.13DD366 pKa = 4.07VSVTHH371 pKa = 6.7TGDD374 pKa = 3.21LTSNAGNGVYY384 pKa = 10.14AYY386 pKa = 8.17TAGGQVDD393 pKa = 3.84VTVNNSILTASGYY406 pKa = 11.16GIFAQTKK413 pKa = 7.66GTAVDD418 pKa = 4.01DD419 pKa = 4.39DD420 pKa = 4.17VTVEE424 pKa = 4.03FNGTIAQSWIGILAEE439 pKa = 4.49ANTGTVLVTSSGEE452 pKa = 3.58IAAWDD457 pKa = 3.66HH458 pKa = 6.55GIWAANTGGGNVTVSQTGGIDD479 pKa = 3.0AGTGHH484 pKa = 8.04AIYY487 pKa = 9.83AYY489 pKa = 9.07SPHH492 pKa = 6.45GAVSVTATGDD502 pKa = 3.35MFRR505 pKa = 11.84SGMTAFYY512 pKa = 11.21VEE514 pKa = 4.61TKK516 pKa = 10.06SGNAATVNVTGDD528 pKa = 3.25IVSGDD533 pKa = 3.53GGITALSAQGTVTVTMRR550 pKa = 11.84GDD552 pKa = 2.99IDD554 pKa = 3.89AGADD558 pKa = 3.75GIYY561 pKa = 10.7AEE563 pKa = 5.23SKK565 pKa = 11.1DD566 pKa = 3.65NDD568 pKa = 3.92LVKK571 pKa = 10.64VDD573 pKa = 4.31TIGDD577 pKa = 3.54ITAGGSAIFAASSQGVVDD595 pKa = 3.87VDD597 pKa = 4.6SIGNLSAGGYY607 pKa = 9.88GIYY610 pKa = 10.26AANLGDD616 pKa = 3.85NVVTVNSTGNIDD628 pKa = 3.21AGNRR632 pKa = 11.84GIHH635 pKa = 5.86AQSATQAVTIVTSGAVTSGSDD656 pKa = 3.16AVFAEE661 pKa = 4.3SLGGNIVSITSIGNLVAGTDD681 pKa = 3.38GDD683 pKa = 4.2GIYY686 pKa = 10.7AFSATGTVTVTSTGDD701 pKa = 2.8IDD703 pKa = 5.32AGDD706 pKa = 4.26DD707 pKa = 4.22GIFARR712 pKa = 11.84STGGQDD718 pKa = 3.44VSVTSLGDD726 pKa = 3.05IGAGTGYY733 pKa = 10.87GIYY736 pKa = 10.27GYY738 pKa = 10.05SATGAVTIGSTGEE751 pKa = 4.14IVSGDD756 pKa = 3.34HH757 pKa = 7.53GIYY760 pKa = 10.52ALNLGYY766 pKa = 8.96GTVGVTQVGDD776 pKa = 3.29IDD778 pKa = 4.36AGGSGIRR785 pKa = 11.84ATSTAGIVSVSNKK798 pKa = 10.01GNIDD802 pKa = 3.38AGGDD806 pKa = 3.6GIYY809 pKa = 10.83ALTLGYY815 pKa = 9.94EE816 pKa = 4.21AATVTNEE823 pKa = 4.24GYY825 pKa = 10.19IDD827 pKa = 3.71ANGYY831 pKa = 9.23GIHH834 pKa = 6.01GHH836 pKa = 6.05SDD838 pKa = 3.11AGSVTIDD845 pKa = 3.17NKK847 pKa = 9.74GAIWSGSDD855 pKa = 3.75GIRR858 pKa = 11.84ATTLSSSLVKK868 pKa = 9.58VTQLGDD874 pKa = 3.45IDD876 pKa = 3.92SGGYY880 pKa = 9.89GVFVNAEE887 pKa = 4.17AGDD890 pKa = 3.96ADD892 pKa = 4.17IDD894 pKa = 3.99TTGDD898 pKa = 3.12ITSVNHH904 pKa = 7.0GIYY907 pKa = 10.73VLTNGNTLIDD917 pKa = 3.59IDD919 pKa = 4.02HH920 pKa = 7.08RR921 pKa = 11.84GSITSTSGNGITATSVAQATIDD943 pKa = 3.59VSVSGGTVSGALDD956 pKa = 4.83GIRR959 pKa = 11.84LASEE963 pKa = 4.21RR964 pKa = 11.84EE965 pKa = 3.83MTVTVGADD973 pKa = 3.05ASVIGGAGNAGVRR986 pKa = 11.84FVEE989 pKa = 4.3GLGIQLTNRR998 pKa = 11.84GSISNAGGIDD1008 pKa = 2.92EE1009 pKa = 4.26WAIVSAQNDD1018 pKa = 4.02TTVDD1022 pKa = 3.26NYY1024 pKa = 10.19GTIAGNVLLGPWSNPFNNFAGALFNMGSTVNIGADD1059 pKa = 3.37STLSNHH1065 pKa = 5.77GTLSPGGEE1073 pKa = 3.84GVVQTTTLTGILVNEE1088 pKa = 4.26ATGTLLFDD1096 pKa = 3.84VDD1098 pKa = 4.08IEE1100 pKa = 4.27NATTDD1105 pKa = 3.63RR1106 pKa = 11.84LNVTNTAALDD1116 pKa = 3.65GNLRR1120 pKa = 11.84LNFVSATGTPGSYY1133 pKa = 10.2TIVTTGEE1140 pKa = 4.4GVTSQSLTLLNPFVLADD1157 pKa = 3.15IATVNNGNDD1166 pKa = 3.36VEE1168 pKa = 4.37LSIHH1172 pKa = 6.33GFDD1175 pKa = 4.85FSPAGMSEE1183 pKa = 4.05NASSIGNAIQTSIQGNGALEE1203 pKa = 4.72PIAVALLNLQTPEE1216 pKa = 3.84EE1217 pKa = 4.53AEE1219 pKa = 4.14DD1220 pKa = 3.87AFEE1223 pKa = 3.92QLSPNTYY1230 pKa = 9.77VADD1233 pKa = 3.8QIGSVDD1239 pKa = 4.33SIATFSNSMLSCRR1252 pKa = 11.84MASGEE1257 pKa = 4.09NAFGAEE1263 pKa = 4.92GEE1265 pKa = 4.71CAWGRR1270 pKa = 11.84AVYY1273 pKa = 9.94SAYY1276 pKa = 10.74DD1277 pKa = 3.55LAANGDD1283 pKa = 3.53NSGFNSRR1290 pKa = 11.84SVEE1293 pKa = 3.93MMGGVQVAVPDD1304 pKa = 4.03TAWRR1308 pKa = 11.84LGGSVGFQASEE1319 pKa = 3.62QDD1321 pKa = 3.7GNNGSSSEE1329 pKa = 4.07GSSFTVGAVAKK1340 pKa = 8.05YY1341 pKa = 10.74APGPYY1346 pKa = 10.05LFAASVAASRR1356 pKa = 11.84GNYY1359 pKa = 7.69DD1360 pKa = 3.39TLRR1363 pKa = 11.84PIDD1366 pKa = 3.76IGNFTDD1372 pKa = 4.07LLSGEE1377 pKa = 4.39TDD1379 pKa = 3.16VTTVSGRR1386 pKa = 11.84LRR1388 pKa = 11.84AAYY1391 pKa = 9.25TMEE1394 pKa = 3.61QGAFYY1399 pKa = 10.22LRR1401 pKa = 11.84PMVDD1405 pKa = 3.35ASATYY1410 pKa = 10.49VRR1412 pKa = 11.84TGAFTEE1418 pKa = 4.4TGGVASLSVGAVEE1431 pKa = 4.21NTVLALMPAIEE1442 pKa = 4.55IGGQVSVGEE1451 pKa = 4.3DD1452 pKa = 3.17MLLRR1456 pKa = 11.84PYY1458 pKa = 10.81LRR1460 pKa = 11.84GGASVYY1466 pKa = 10.76SGNEE1470 pKa = 3.56YY1471 pKa = 10.94SLTGSFTADD1480 pKa = 2.98GNAAAPFTVTSSSEE1494 pKa = 3.99DD1495 pKa = 3.6VLWTVSTGVDD1505 pKa = 3.77LLRR1508 pKa = 11.84GDD1510 pKa = 3.86VGTLQIFYY1518 pKa = 9.82EE1519 pKa = 4.88GAFGEE1524 pKa = 4.53HH1525 pKa = 5.02TTINSGGAKK1534 pKa = 10.27FSVNFF1539 pKa = 3.9

Molecular weight: 153.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A429YZ32|A0A429YZ32_9RHIZ Uncharacterized protein OS=Mesorhizobium carbonis OX=2495581 GN=EJC49_09135 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11GGRR28 pKa = 11.84GVVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4987

0

4987

1586296

22

6283

318.1

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.77 ± 0.053

0.8 ± 0.012

5.88 ± 0.029

5.714 ± 0.036

3.844 ± 0.025

8.856 ± 0.049

2.037 ± 0.018

5.225 ± 0.027

2.883 ± 0.03

9.861 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.018

2.402 ± 0.022

5.122 ± 0.029

2.801 ± 0.021

7.489 ± 0.044

5.358 ± 0.031

5.25 ± 0.03

7.648 ± 0.031

1.336 ± 0.015

2.131 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski