Gammapapillomavirus 18
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2AM25|A0A2D2AM25_9PAPI Minor capsid protein L2 OS=Gammapapillomavirus 18 OX=1513263 GN=L2 PE=3 SV=1
MM1 pKa = 7.18 SVSRR5 pKa = 11.84 NKK7 pKa = 10.32 RR8 pKa = 11.84 DD9 pKa = 3.62 TVEE12 pKa = 4.29 NLYY15 pKa = 10.59 KK16 pKa = 10.31 SCKK19 pKa = 10.05 LGGDD23 pKa = 3.95 CPPDD27 pKa = 3.3 VVNKK31 pKa = 9.97 VEE33 pKa = 4.12 QKK35 pKa = 9.21 TLADD39 pKa = 3.33 ILLQAFSSVLYY50 pKa = 10.76 LGGLGFGTGKK60 pKa = 10.81 GSTGTVGVRR69 pKa = 11.84 PLPEE73 pKa = 3.97 VPPIRR78 pKa = 11.84 IPTEE82 pKa = 3.95 TVPEE86 pKa = 4.8 EE87 pKa = 4.16 IPLAPIKK94 pKa = 10.17 PKK96 pKa = 10.46 TPITVGQGRR105 pKa = 11.84 RR106 pKa = 11.84 PFSVPLDD113 pKa = 3.73 TIGAGFRR120 pKa = 11.84 PVDD123 pKa = 3.55 PSGSRR128 pKa = 11.84 TIDD131 pKa = 3.44 VIDD134 pKa = 4.32 PSSPSVITPRR144 pKa = 11.84 EE145 pKa = 4.12 SLPDD149 pKa = 3.72 TIITIGQPNLEE160 pKa = 4.37 TGDD163 pKa = 4.1 SVVTDD168 pKa = 4.23 LNIITDD174 pKa = 3.69 TTSIISHH181 pKa = 5.72 PTVIQGVEE189 pKa = 4.06 EE190 pKa = 4.01 NVAILTVTPADD201 pKa = 3.9 PPPTRR206 pKa = 11.84 VKK208 pKa = 9.98 FTLPEE213 pKa = 3.72 QSPNISIEE221 pKa = 3.98 SLAGHH226 pKa = 6.08 VEE228 pKa = 3.62 PTYY231 pKa = 11.22 DD232 pKa = 3.58 VFVDD236 pKa = 4.92 PVASGEE242 pKa = 4.15 DD243 pKa = 3.29 IYY245 pKa = 11.21 FGEE248 pKa = 5.51 DD249 pKa = 3.12 IPLEE253 pKa = 4.47 PINPRR258 pKa = 11.84 LEE260 pKa = 4.2 FEE262 pKa = 4.29 IEE264 pKa = 4.12 EE265 pKa = 4.79 PPQSSTPEE273 pKa = 3.62 QRR275 pKa = 11.84 LQRR278 pKa = 11.84 AFSRR282 pKa = 11.84 ARR284 pKa = 11.84 EE285 pKa = 4.16 FYY287 pKa = 10.38 SRR289 pKa = 11.84 HH290 pKa = 4.52 IQQVRR295 pKa = 11.84 TRR297 pKa = 11.84 NLNLLGDD304 pKa = 3.82 VARR307 pKa = 11.84 AIEE310 pKa = 4.38 FGFEE314 pKa = 3.7 NPAFDD319 pKa = 5.25 SEE321 pKa = 4.22 ITLEE325 pKa = 4.32 FEE327 pKa = 5.23 RR328 pKa = 11.84 EE329 pKa = 4.24 VNDD332 pKa = 4.25 LRR334 pKa = 11.84 AAPDD338 pKa = 3.12 IDD340 pKa = 3.7 FTDD343 pKa = 3.14 IQTISRR349 pKa = 11.84 PYY351 pKa = 10.09 IEE353 pKa = 4.77 STPEE357 pKa = 3.37 GTVRR361 pKa = 11.84 VSRR364 pKa = 11.84 LGTRR368 pKa = 11.84 AGVRR372 pKa = 11.84 TRR374 pKa = 11.84 RR375 pKa = 11.84 GTVLSQNVHH384 pKa = 5.72 YY385 pKa = 10.16 YY386 pKa = 10.85 YY387 pKa = 10.43 DD388 pKa = 3.64 ISPIPSTEE396 pKa = 4.15 TIEE399 pKa = 5.17 LSTFTPEE406 pKa = 4.24 TVVDD410 pKa = 3.6 TSVNPNTEE418 pKa = 3.96 SVFINEE424 pKa = 4.02 IEE426 pKa = 4.13 EE427 pKa = 4.31 LPEE430 pKa = 4.91 DD431 pKa = 4.0 VLLDD435 pKa = 3.68 SYY437 pKa = 11.25 TEE439 pKa = 4.25 SFNDD443 pKa = 3.17 AHH445 pKa = 6.98 LLLEE449 pKa = 4.55 ATDD452 pKa = 4.19 EE453 pKa = 4.14 EE454 pKa = 5.16 GEE456 pKa = 4.28 RR457 pKa = 11.84 YY458 pKa = 9.57 QIPVVASDD466 pKa = 3.61 TVKK469 pKa = 10.65 VFVYY473 pKa = 10.26 DD474 pKa = 3.65 YY475 pKa = 11.12 GSDD478 pKa = 3.57 IIVAEE483 pKa = 4.56 PGSSEE488 pKa = 4.64 SPFPVPNIPLTPLQPPANILIVSDD512 pKa = 4.71 DD513 pKa = 4.32 FYY515 pKa = 11.53 LHH517 pKa = 7.37 PSLQRR522 pKa = 11.84 KK523 pKa = 9.12 RR524 pKa = 11.84 KK525 pKa = 9.4 RR526 pKa = 11.84 KK527 pKa = 9.54 RR528 pKa = 11.84 LDD530 pKa = 3.15 YY531 pKa = 10.84 FF532 pKa = 3.88
Molecular weight: 58.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.593
IPC2_protein 4.507
IPC_protein 4.457
Toseland 4.304
ProMoST 4.558
Dawson 4.406
Bjellqvist 4.558
Wikipedia 4.266
Rodwell 4.304
Grimsley 4.215
Solomon 4.406
Lehninger 4.355
Nozaki 4.507
DTASelect 4.673
Thurlkill 4.304
EMBOSS 4.291
Sillero 4.571
Patrickios 3.973
IPC_peptide 4.406
IPC2_peptide 4.558
IPC2.peptide.svr19 4.513
Protein with the highest isoelectric point:
>tr|A0A2D2ALY4|A0A2D2ALY4_9PAPI Protein E7 OS=Gammapapillomavirus 18 OX=1513263 GN=E7 PE=3 SV=1
MM1 pKa = 7.01 NQADD5 pKa = 3.91 LTKK8 pKa = 10.64 RR9 pKa = 11.84 SDD11 pKa = 3.51 ALQEE15 pKa = 3.97 RR16 pKa = 11.84 LMNLYY21 pKa = 10.39 EE22 pKa = 4.71 EE23 pKa = 5.11 GAQTIDD29 pKa = 3.53 AQIEE33 pKa = 3.79 HH34 pKa = 6.52 WKK36 pKa = 10.21 IIKK39 pKa = 8.71 QQYY42 pKa = 6.29 ITYY45 pKa = 9.3 YY46 pKa = 8.2 YY47 pKa = 10.64 ARR49 pKa = 11.84 KK50 pKa = 9.88 EE51 pKa = 4.16 GFKK54 pKa = 10.93 KK55 pKa = 10.59 LGLQILPTYY64 pKa = 10.24 VVSEE68 pKa = 4.28 YY69 pKa = 10.22 KK70 pKa = 10.6 AKK72 pKa = 10.54 EE73 pKa = 4.11 AIQQILLLEE82 pKa = 4.25 SLKK85 pKa = 10.7 KK86 pKa = 10.49 SQFGNEE92 pKa = 3.75 PWTLSEE98 pKa = 4.22 TSAEE102 pKa = 4.18 LTLTPPRR109 pKa = 11.84 YY110 pKa = 9.81 SFKK113 pKa = 10.45 KK114 pKa = 9.82 DD115 pKa = 3.32 PYY117 pKa = 11.1 VVDD120 pKa = 3.09 VWFDD124 pKa = 3.83 NNPEE128 pKa = 3.84 NSFPYY133 pKa = 9.96 TNWNKK138 pKa = 9.98 IYY140 pKa = 10.52 YY141 pKa = 9.48 QDD143 pKa = 4.16 EE144 pKa = 4.12 QEE146 pKa = 3.95 HH147 pKa = 4.4 WHH149 pKa = 5.7 RR150 pKa = 11.84 AEE152 pKa = 4.15 GKK154 pKa = 9.59 VDD156 pKa = 3.21 INGLYY161 pKa = 10.3 FVDD164 pKa = 3.89 INGDD168 pKa = 3.04 RR169 pKa = 11.84 NYY171 pKa = 10.5 FVVFAPDD178 pKa = 3.17 VEE180 pKa = 4.83 RR181 pKa = 11.84 YY182 pKa = 7.51 STTGLWTVNFKK193 pKa = 10.68 NQTISSVDD201 pKa = 3.26 SSQTRR206 pKa = 11.84 VSDD209 pKa = 3.51 ISPQGSVSSSRR220 pKa = 11.84 DD221 pKa = 3.35 TIQRR225 pKa = 11.84 PTTTSSRR232 pKa = 11.84 GSEE235 pKa = 4.06 GEE237 pKa = 3.73 EE238 pKa = 3.63 RR239 pKa = 11.84 SPHH242 pKa = 5.17 STTPSSPDD250 pKa = 2.94 LRR252 pKa = 11.84 GGQRR256 pKa = 11.84 RR257 pKa = 11.84 RR258 pKa = 11.84 RR259 pKa = 11.84 QRR261 pKa = 11.84 EE262 pKa = 3.51 SSPRR266 pKa = 11.84 GRR268 pKa = 11.84 SKK270 pKa = 10.59 RR271 pKa = 11.84 RR272 pKa = 11.84 RR273 pKa = 11.84 EE274 pKa = 3.87 EE275 pKa = 3.74 EE276 pKa = 3.89 ATSGVAPGEE285 pKa = 4.22 VGRR288 pKa = 11.84 GSVTVPRR295 pKa = 11.84 RR296 pKa = 11.84 GLGRR300 pKa = 11.84 IEE302 pKa = 4.39 RR303 pKa = 11.84 LTEE306 pKa = 3.72 EE307 pKa = 4.87 ARR309 pKa = 11.84 DD310 pKa = 3.64 PPIIFVKK317 pKa = 10.83 GGANNLKK324 pKa = 9.87 CWRR327 pKa = 11.84 NRR329 pKa = 11.84 CQKK332 pKa = 8.97 TNRR335 pKa = 11.84 LYY337 pKa = 10.62 TVMSTVFRR345 pKa = 11.84 YY346 pKa = 9.48 PGTEE350 pKa = 3.44 LGHH353 pKa = 6.64 RR354 pKa = 11.84 MVIGFRR360 pKa = 11.84 DD361 pKa = 3.71 FDD363 pKa = 3.59 QRR365 pKa = 11.84 TLFLKK370 pKa = 10.03 TITFPKK376 pKa = 9.84 GCTYY380 pKa = 10.65 SLGRR384 pKa = 11.84 IDD386 pKa = 4.88 SLL388 pKa = 4.34
Molecular weight: 44.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.806
IPC2_protein 8.829
IPC_protein 8.814
Toseland 9.282
ProMoST 9.18
Dawson 9.633
Bjellqvist 9.399
Wikipedia 9.838
Rodwell 9.78
Grimsley 9.721
Solomon 9.663
Lehninger 9.619
Nozaki 9.385
DTASelect 9.37
Thurlkill 9.443
EMBOSS 9.736
Sillero 9.575
Patrickios 4.774
IPC_peptide 9.648
IPC2_peptide 8.053
IPC2.peptide.svr19 7.814
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2258
96
597
376.3
42.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.606 ± 0.367
2.834 ± 1.293
6.289 ± 0.432
6.82 ± 0.607
5.049 ± 0.62
5.27 ± 0.639
1.284 ± 0.134
5.624 ± 0.699
5.359 ± 0.985
8.902 ± 0.967
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.55 ± 0.451
5.226 ± 0.606
6.289 ± 1.327
4.429 ± 0.433
5.802 ± 0.936
6.776 ± 0.81
6.643 ± 0.725
6.289 ± 0.732
1.151 ± 0.375
3.809 ± 0.63
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here