Edwardsiella phage vB_EtaM_ET-ABTNL-9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8D3B5|A0A5P8D3B5_9CAUD Structural protein OS=Edwardsiella phage vB_EtaM_ET-ABTNL-9 OX=2651067 PE=4 SV=1
MM1 pKa = 7.06YY2 pKa = 10.19KK3 pKa = 10.38VVINRR8 pKa = 11.84CFGGFSLSKK17 pKa = 10.3EE18 pKa = 3.52ASEE21 pKa = 4.36YY22 pKa = 11.35LNEE25 pKa = 4.48KK26 pKa = 9.65YY27 pKa = 10.69NLGIDD32 pKa = 3.57PEE34 pKa = 4.24YY35 pKa = 11.14GYY37 pKa = 11.35LSDD40 pKa = 6.1LDD42 pKa = 4.18DD43 pKa = 4.97DD44 pKa = 5.02NYY46 pKa = 10.86VPRR49 pKa = 11.84HH50 pKa = 5.98DD51 pKa = 3.75KK52 pKa = 10.9RR53 pKa = 11.84LIEE56 pKa = 4.1VVEE59 pKa = 4.06TLGEE63 pKa = 4.07DD64 pKa = 3.61RR65 pKa = 11.84ASGDD69 pKa = 3.83CSDD72 pKa = 4.33LSIVEE77 pKa = 4.23ISTPCYY83 pKa = 10.43RR84 pKa = 11.84IDD86 pKa = 4.07EE87 pKa = 4.37YY88 pKa = 11.65DD89 pKa = 3.24GMEE92 pKa = 4.14SVEE95 pKa = 4.18TPEE98 pKa = 5.13DD99 pKa = 3.68IEE101 pKa = 3.81WEE103 pKa = 4.28YY104 pKa = 10.82IHH106 pKa = 6.92EE107 pKa = 4.7GEE109 pKa = 4.62

Molecular weight: 12.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8D5H1|A0A5P8D5H1_9CAUD Uncharacterized protein OS=Edwardsiella phage vB_EtaM_ET-ABTNL-9 OX=2651067 PE=4 SV=1
MM1 pKa = 7.18VSSIMCLTLMVFHH14 pKa = 7.14EE15 pKa = 5.25AGNQPVKK22 pKa = 11.06GKK24 pKa = 8.75MATLEE29 pKa = 4.24VVQNRR34 pKa = 11.84VNSDD38 pKa = 3.21KK39 pKa = 11.1YY40 pKa = 10.23PNSHH44 pKa = 6.84CSVIRR49 pKa = 11.84QKK51 pKa = 11.17NQFSWVNSSNRR62 pKa = 11.84ALNKK66 pKa = 9.94IPNKK70 pKa = 9.86VKK72 pKa = 10.71NSPTYY77 pKa = 9.0KK78 pKa = 10.15RR79 pKa = 11.84QWEE82 pKa = 4.21EE83 pKa = 3.5SQEE86 pKa = 4.01AVKK89 pKa = 10.73KK90 pKa = 10.11FLKK93 pKa = 10.8SPTNYY98 pKa = 9.71TKK100 pKa = 10.65GAHH103 pKa = 5.37YY104 pKa = 10.53FNTNGLGVRR113 pKa = 11.84YY114 pKa = 7.36KK115 pKa = 11.0TNVKK119 pKa = 8.68PVKK122 pKa = 10.17IGGHH126 pKa = 4.55TFYY129 pKa = 11.41

Molecular weight: 14.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

18947

58

1878

291.5

32.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.555 ± 0.592

1.071 ± 0.174

6.587 ± 0.193

7.563 ± 0.333

3.758 ± 0.252

6.65 ± 0.3

1.647 ± 0.138

6.122 ± 0.219

7.859 ± 0.297

8.123 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.259 ± 0.118

5.304 ± 0.23

2.898 ± 0.223

3.246 ± 0.156

4.296 ± 0.142

7.4 ± 0.256

6.355 ± 0.327

6.993 ± 0.22

1.151 ± 0.119

4.164 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski