Grapevine fleck virus (isolate Italy/MT48) (GFkV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Tymoviridae; Maculavirus; Grapevine fleck virus

Average proteome isoelectric point is 8.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q8UZB6|RDRP_GFKVM RNA replication protein OS=Grapevine fleck virus (isolate Italy/MT48) OX=652668 PE=4 SV=1
MM1 pKa = 7.56SLPADD6 pKa = 4.19LLLGAISSLLRR17 pKa = 11.84NPPTSDD23 pKa = 3.13AASPSADD30 pKa = 3.21QPAVSSSRR38 pKa = 11.84SDD40 pKa = 3.2SRR42 pKa = 11.84LVSAPLPAAPPAPTAIARR60 pKa = 11.84NPRR63 pKa = 11.84VSIHH67 pKa = 6.96LPFQFLWYY75 pKa = 9.71DD76 pKa = 3.15ITGTEE81 pKa = 3.7SSYY84 pKa = 11.12TSLSIASRR92 pKa = 11.84PEE94 pKa = 3.82VVTVARR100 pKa = 11.84PYY102 pKa = 9.56RR103 pKa = 11.84HH104 pKa = 6.52ARR106 pKa = 11.84LTSLEE111 pKa = 4.45AFVQPTASSATYY123 pKa = 8.56PQTVDD128 pKa = 3.54LCWTIDD134 pKa = 3.35SVTPARR140 pKa = 11.84SEE142 pKa = 3.81ILSVFGAQRR151 pKa = 11.84IAWGSVHH158 pKa = 6.78FSAPILLPAEE168 pKa = 4.43LSSLNPTIKK177 pKa = 10.73DD178 pKa = 3.34SVTYY182 pKa = 9.14TDD184 pKa = 5.3CPRR187 pKa = 11.84LTCGFYY193 pKa = 10.9RR194 pKa = 11.84NDD196 pKa = 3.09ACVALGSSAPICGSILIRR214 pKa = 11.84GVIEE218 pKa = 3.92CSAPINRR225 pKa = 11.84PTPSSS230 pKa = 3.3

Molecular weight: 24.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q8UZB4|ORF3_GFKVM Uncharacterized protein ORF3 OS=Grapevine fleck virus (isolate Italy/MT48) OX=652668 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.32NRR3 pKa = 11.84GPPLRR8 pKa = 11.84SRR10 pKa = 11.84PPSSPPPASAFPGPSPFPSPSPANSLPSASPPPPTCTPSSPVSRR54 pKa = 11.84PFASARR60 pKa = 11.84LRR62 pKa = 11.84TSHH65 pKa = 7.5PPRR68 pKa = 11.84CPHH71 pKa = 6.88RR72 pKa = 11.84SAPPSAPSPPFTPPHH87 pKa = 6.68PLPTPTPSSSPRR99 pKa = 11.84SPWLSLAPLPTSSASLASFPPPPSSFSSPSSPSTSPLSPSSSSFPSSSSFSFLVPSNSS157 pKa = 3.04

Molecular weight: 15.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2645

157

1949

661.3

71.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.603 ± 0.614

1.172 ± 0.303

3.289 ± 0.925

2.457 ± 0.755

4.688 ± 0.884

3.138 ± 0.735

3.781 ± 1.136

3.516 ± 0.896

1.701 ± 0.667

12.703 ± 1.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.567 ± 0.03

2.155 ± 0.414

16.295 ± 5.454

2.533 ± 0.837

6.238 ± 0.222

10.888 ± 4.771

7.486 ± 0.808

4.423 ± 0.851

1.134 ± 0.327

2.231 ± 0.723
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski