Amino acid dipepetide frequency for Rhodomicrobium vannielii (strain ATCC 17100 / ATH 3.1.1 / DSM 162 / LMG 4299)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.155AlaAla: 18.155 ± 0.193
1.148AlaCys: 1.148 ± 0.037
6.774AlaAsp: 6.774 ± 0.096
8.014AlaGlu: 8.014 ± 0.112
4.919AlaPhe: 4.919 ± 0.076
9.59AlaGly: 9.59 ± 0.102
2.24AlaHis: 2.24 ± 0.041
6.602AlaIle: 6.602 ± 0.075
5.052AlaLys: 5.052 ± 0.079
13.76AlaLeu: 13.76 ± 0.141
3.076AlaMet: 3.076 ± 0.058
3.044AlaAsn: 3.044 ± 0.051
6.082AlaPro: 6.082 ± 0.1
3.994AlaGln: 3.994 ± 0.074
8.929AlaArg: 8.929 ± 0.118
7.238AlaSer: 7.238 ± 0.102
6.03AlaThr: 6.03 ± 0.146
8.523AlaVal: 8.523 ± 0.091
1.448AlaTrp: 1.448 ± 0.035
2.691AlaTyr: 2.691 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
1.023CysAla: 1.023 ± 0.035
0.115CysCys: 0.115 ± 0.012
0.529CysAsp: 0.529 ± 0.026
0.497CysGlu: 0.497 ± 0.02
0.351CysPhe: 0.351 ± 0.018
0.922CysGly: 0.922 ± 0.034
0.245CysHis: 0.245 ± 0.019
0.419CysIle: 0.419 ± 0.021
0.276CysLys: 0.276 ± 0.019
0.854CysLeu: 0.854 ± 0.026
0.172CysMet: 0.172 ± 0.011
0.245CysAsn: 0.245 ± 0.016
0.5CysPro: 0.5 ± 0.025
0.234CysGln: 0.234 ± 0.014
0.616CysArg: 0.616 ± 0.024
0.496CysSer: 0.496 ± 0.024
0.375CysThr: 0.375 ± 0.018
0.689CysVal: 0.689 ± 0.023
0.109CysTrp: 0.109 ± 0.01
0.215CysTyr: 0.215 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
7.32AspAla: 7.32 ± 0.102
0.483AspCys: 0.483 ± 0.022
3.018AspAsp: 3.018 ± 0.067
3.692AspGlu: 3.692 ± 0.063
2.297AspPhe: 2.297 ± 0.046
4.843AspGly: 4.843 ± 0.069
1.097AspHis: 1.097 ± 0.032
3.123AspIle: 3.123 ± 0.056
2.023AspLys: 2.023 ± 0.05
5.566AspLeu: 5.566 ± 0.078
1.141AspMet: 1.141 ± 0.033
1.286AspAsn: 1.286 ± 0.04
2.953AspPro: 2.953 ± 0.052
1.458AspGln: 1.458 ± 0.042
3.947AspArg: 3.947 ± 0.063
2.139AspSer: 2.139 ± 0.048
2.535AspThr: 2.535 ± 0.051
4.081AspVal: 4.081 ± 0.054
0.838AspTrp: 0.838 ± 0.029
1.517AspTyr: 1.517 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
8.399GluAla: 8.399 ± 0.11
0.399GluCys: 0.399 ± 0.018
2.968GluAsp: 2.968 ± 0.055
3.393GluGlu: 3.393 ± 0.075
1.821GluPhe: 1.821 ± 0.04
4.451GluGly: 4.451 ± 0.061
1.212GluHis: 1.212 ± 0.032
3.533GluIle: 3.533 ± 0.061
2.978GluLys: 2.978 ± 0.059
5.259GluLeu: 5.259 ± 0.069
1.397GluMet: 1.397 ± 0.041
1.732GluAsn: 1.732 ± 0.044
3.062GluPro: 3.062 ± 0.06
1.992GluGln: 1.992 ± 0.049
5.416GluArg: 5.416 ± 0.082
2.54GluSer: 2.54 ± 0.05
3.831GluThr: 3.831 ± 0.057
3.881GluVal: 3.881 ± 0.064
0.738GluTrp: 0.738 ± 0.027
1.004GluTyr: 1.004 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.912PheAla: 4.912 ± 0.075
0.459PheCys: 0.459 ± 0.02
2.623PheAsp: 2.623 ± 0.05
2.445PheGlu: 2.445 ± 0.051
1.61PhePhe: 1.61 ± 0.038
3.669PheGly: 3.669 ± 0.056
0.795PheHis: 0.795 ± 0.025
1.889PheIle: 1.889 ± 0.04
1.208PheLys: 1.208 ± 0.033
3.581PheLeu: 3.581 ± 0.062
0.782PheMet: 0.782 ± 0.027
1.051PheAsn: 1.051 ± 0.03
1.668PhePro: 1.668 ± 0.042
1.058PheGln: 1.058 ± 0.034
2.535PheArg: 2.535 ± 0.052
2.32PheSer: 2.32 ± 0.043
1.958PheThr: 1.958 ± 0.044
2.886PheVal: 2.886 ± 0.055
0.524PheTrp: 0.524 ± 0.025
0.97PheTyr: 0.97 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
9.292GlyAla: 9.292 ± 0.162
0.867GlyCys: 0.867 ± 0.032
4.425GlyAsp: 4.425 ± 0.075
4.979GlyGlu: 4.979 ± 0.078
3.635GlyPhe: 3.635 ± 0.056
7.261GlyGly: 7.261 ± 0.118
1.714GlyHis: 1.714 ± 0.04
4.302GlyIle: 4.302 ± 0.079
3.528GlyLys: 3.528 ± 0.066
8.003GlyLeu: 8.003 ± 0.087
1.741GlyMet: 1.741 ± 0.045
2.174GlyAsn: 2.174 ± 0.091
3.17GlyPro: 3.17 ± 0.06
2.619GlyGln: 2.619 ± 0.052
5.759GlyArg: 5.759 ± 0.076
4.515GlySer: 4.515 ± 0.085
4.146GlyThr: 4.146 ± 0.069
6.217GlyVal: 6.217 ± 0.07
1.205GlyTrp: 1.205 ± 0.036
2.206GlyTyr: 2.206 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
2.329HisAla: 2.329 ± 0.046
0.244HisCys: 0.244 ± 0.015
1.092HisAsp: 1.092 ± 0.034
1.126HisGlu: 1.126 ± 0.034
0.853HisPhe: 0.853 ± 0.031
1.816HisGly: 1.816 ± 0.043
0.509HisHis: 0.509 ± 0.027
1.025HisIle: 1.025 ± 0.031
0.603HisLys: 0.603 ± 0.025
2.011HisLeu: 2.011 ± 0.05
0.466HisMet: 0.466 ± 0.023
0.486HisAsn: 0.486 ± 0.02
1.247HisPro: 1.247 ± 0.036
0.522HisGln: 0.522 ± 0.021
1.435HisArg: 1.435 ± 0.036
0.97HisSer: 0.97 ± 0.032
0.862HisThr: 0.862 ± 0.027
1.457HisVal: 1.457 ± 0.038
0.309HisTrp: 0.309 ± 0.017
0.565HisTyr: 0.565 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
7.43IleAla: 7.43 ± 0.083
0.505IleCys: 0.505 ± 0.021
3.314IleAsp: 3.314 ± 0.065
3.843IleGlu: 3.843 ± 0.064
1.791IlePhe: 1.791 ± 0.038
4.613IleGly: 4.613 ± 0.075
0.933IleHis: 0.933 ± 0.03
2.308IleIle: 2.308 ± 0.052
1.746IleLys: 1.746 ± 0.043
4.585IleLeu: 4.585 ± 0.075
0.99IleMet: 0.99 ± 0.033
1.375IleAsn: 1.375 ± 0.036
2.434IlePro: 2.434 ± 0.049
1.207IleGln: 1.207 ± 0.035
3.318IleArg: 3.318 ± 0.057
2.892IleSer: 2.892 ± 0.062
2.53IleThr: 2.53 ± 0.052
4.326IleVal: 4.326 ± 0.067
0.608IleTrp: 0.608 ± 0.023
1.212IleTyr: 1.212 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
5.235LysAla: 5.235 ± 0.079
0.225LysCys: 0.225 ± 0.014
2.033LysAsp: 2.033 ± 0.044
2.11LysGlu: 2.11 ± 0.047
1.067LysPhe: 1.067 ± 0.036
3.023LysGly: 3.023 ± 0.058
0.742LysHis: 0.742 ± 0.025
2.022LysIle: 2.022 ± 0.045
1.92LysLys: 1.92 ± 0.055
3.957LysLeu: 3.957 ± 0.076
0.763LysMet: 0.763 ± 0.023
1.084LysAsn: 1.084 ± 0.03
2.734LysPro: 2.734 ± 0.051
1.166LysGln: 1.166 ± 0.033
2.942LysArg: 2.942 ± 0.057
2.24LysSer: 2.24 ± 0.046
2.45LysThr: 2.45 ± 0.048
2.564LysVal: 2.564 ± 0.05
0.41LysTrp: 0.41 ± 0.02
0.769LysTyr: 0.769 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
13.434LeuAla: 13.434 ± 0.142
0.926LeuCys: 0.926 ± 0.031
5.863LeuAsp: 5.863 ± 0.088
5.309LeuGlu: 5.309 ± 0.068
3.822LeuPhe: 3.822 ± 0.066
7.828LeuGly: 7.828 ± 0.102
1.892LeuHis: 1.892 ± 0.04
5.082LeuIle: 5.082 ± 0.073
4.374LeuLys: 4.374 ± 0.071
8.965LeuLeu: 8.965 ± 0.117
2.015LeuMet: 2.015 ± 0.049
2.693LeuAsn: 2.693 ± 0.053
5.499LeuPro: 5.499 ± 0.069
2.685LeuGln: 2.685 ± 0.048
6.85LeuArg: 6.85 ± 0.083
6.646LeuSer: 6.646 ± 0.088
5.268LeuThr: 5.268 ± 0.078
6.755LeuVal: 6.755 ± 0.086
1.051LeuTrp: 1.051 ± 0.034
2.159LeuTyr: 2.159 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
2.613MetAla: 2.613 ± 0.059
0.158MetCys: 0.158 ± 0.013
0.972MetAsp: 0.972 ± 0.027
1.008MetGlu: 1.008 ± 0.027
0.632MetPhe: 0.632 ± 0.023
1.5MetGly: 1.5 ± 0.04
0.449MetHis: 0.449 ± 0.018
1.178MetIle: 1.178 ± 0.032
0.98MetLys: 0.98 ± 0.03
2.076MetLeu: 2.076 ± 0.046
0.537MetMet: 0.537 ± 0.026
0.66MetAsn: 0.66 ± 0.024
1.376MetPro: 1.376 ± 0.039
0.756MetGln: 0.756 ± 0.027
1.776MetArg: 1.776 ± 0.035
1.41MetSer: 1.41 ± 0.036
1.591MetThr: 1.591 ± 0.04
1.305MetVal: 1.305 ± 0.035
0.183MetTrp: 0.183 ± 0.013
0.269MetTyr: 0.269 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.505AsnAla: 3.505 ± 0.056
0.288AsnCys: 0.288 ± 0.018
1.384AsnAsp: 1.384 ± 0.035
1.435AsnGlu: 1.435 ± 0.039
0.985AsnPhe: 0.985 ± 0.031
2.466AsnGly: 2.466 ± 0.053
0.458AsnHis: 0.458 ± 0.02
1.467AsnIle: 1.467 ± 0.036
0.821AsnLys: 0.821 ± 0.032
2.474AsnLeu: 2.474 ± 0.054
0.583AsnMet: 0.583 ± 0.022
0.727AsnAsn: 0.727 ± 0.032
1.823AsnPro: 1.823 ± 0.038
0.713AsnGln: 0.713 ± 0.027
1.925AsnArg: 1.925 ± 0.04
1.284AsnSer: 1.284 ± 0.041
1.32AsnThr: 1.32 ± 0.041
1.902AsnVal: 1.902 ± 0.044
0.407AsnTrp: 0.407 ± 0.019
0.73AsnTyr: 0.73 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
6.156ProAla: 6.156 ± 0.104
0.385ProCys: 0.385 ± 0.018
3.409ProAsp: 3.409 ± 0.056
3.756ProGlu: 3.756 ± 0.056
2.131ProPhe: 2.131 ± 0.045
4.3ProGly: 4.3 ± 0.068
1.112ProHis: 1.112 ± 0.033
2.275ProIle: 2.275 ± 0.05
2.179ProLys: 2.179 ± 0.047
4.847ProLeu: 4.847 ± 0.064
1.012ProMet: 1.012 ± 0.027
1.379ProAsn: 1.379 ± 0.037
2.726ProPro: 2.726 ± 0.075
1.781ProGln: 1.781 ± 0.043
3.134ProArg: 3.134 ± 0.064
3.029ProSer: 3.029 ± 0.049
2.37ProThr: 2.37 ± 0.045
4.207ProVal: 4.207 ± 0.066
0.662ProTrp: 0.662 ± 0.029
1.269ProTyr: 1.269 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
3.704GlnAla: 3.704 ± 0.065
0.191GlnCys: 0.191 ± 0.014
1.318GlnAsp: 1.318 ± 0.037
1.513GlnGlu: 1.513 ± 0.038
1.08GlnPhe: 1.08 ± 0.03
2.266GlnGly: 2.266 ± 0.05
0.634GlnHis: 0.634 ± 0.026
1.72GlnIle: 1.72 ± 0.042
1.361GlnLys: 1.361 ± 0.039
2.733GlnLeu: 2.733 ± 0.053
0.738GlnMet: 0.738 ± 0.026
0.947GlnAsn: 0.947 ± 0.029
1.825GlnPro: 1.825 ± 0.053
1.154GlnGln: 1.154 ± 0.041
2.392GlnArg: 2.392 ± 0.055
1.83GlnSer: 1.83 ± 0.047
1.79GlnThr: 1.79 ± 0.046
2.009GlnVal: 2.009 ± 0.045
0.339GlnTrp: 0.339 ± 0.019
0.591GlnTyr: 0.591 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
8.397ArgAla: 8.397 ± 0.095
0.559ArgCys: 0.559 ± 0.023
4.159ArgAsp: 4.159 ± 0.07
4.624ArgGlu: 4.624 ± 0.07
3.163ArgPhe: 3.163 ± 0.064
4.912ArgGly: 4.912 ± 0.076
1.681ArgHis: 1.681 ± 0.045
3.945ArgIle: 3.945 ± 0.074
2.613ArgLys: 2.613 ± 0.053
7.87ArgLeu: 7.87 ± 0.101
1.711ArgMet: 1.711 ± 0.037
1.849ArgAsn: 1.849 ± 0.045
3.351ArgPro: 3.351 ± 0.061
2.413ArgGln: 2.413 ± 0.048
5.682ArgArg: 5.682 ± 0.102
3.811ArgSer: 3.811 ± 0.06
3.258ArgThr: 3.258 ± 0.051
4.95ArgVal: 4.95 ± 0.073
0.926ArgTrp: 0.926 ± 0.028
1.834ArgTyr: 1.834 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
6.634SerAla: 6.634 ± 0.083
0.46SerCys: 0.46 ± 0.022
3.007SerAsp: 3.007 ± 0.054
3.18SerGlu: 3.18 ± 0.055
2.396SerPhe: 2.396 ± 0.052
5.504SerGly: 5.504 ± 0.11
1.123SerHis: 1.123 ± 0.034
2.8SerIle: 2.8 ± 0.052
1.9SerLys: 1.9 ± 0.049
5.663SerLeu: 5.663 ± 0.072
1.088SerMet: 1.088 ± 0.028
1.485SerAsn: 1.485 ± 0.043
3.004SerPro: 3.004 ± 0.059
1.692SerGln: 1.692 ± 0.036
3.879SerArg: 3.879 ± 0.069
3.32SerSer: 3.32 ± 0.056
2.748SerThr: 2.748 ± 0.058
4.113SerVal: 4.113 ± 0.067
0.753SerTrp: 0.753 ± 0.026
1.338SerTyr: 1.338 ± 0.034
0.0SerXaa: 0.0 ± 0.0
Thr
6.112ThrAla: 6.112 ± 0.077
0.425ThrCys: 0.425 ± 0.02
2.516ThrAsp: 2.516 ± 0.044
2.611ThrGlu: 2.611 ± 0.052
2.043ThrPhe: 2.043 ± 0.04
4.836ThrGly: 4.836 ± 0.184
1.002ThrHis: 1.002 ± 0.03
2.773ThrIle: 2.773 ± 0.055
1.804ThrLys: 1.804 ± 0.044
5.825ThrLeu: 5.825 ± 0.075
0.985ThrMet: 0.985 ± 0.032
1.374ThrAsn: 1.374 ± 0.04
3.196ThrPro: 3.196 ± 0.057
1.42ThrGln: 1.42 ± 0.037
3.231ThrArg: 3.231 ± 0.048
2.931ThrSer: 2.931 ± 0.063
2.562ThrThr: 2.562 ± 0.066
4.148ThrVal: 4.148 ± 0.065
0.583ThrTrp: 0.583 ± 0.026
1.237ThrTyr: 1.237 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
8.933ValAla: 8.933 ± 0.092
0.657ValCys: 0.657 ± 0.025
3.909ValAsp: 3.909 ± 0.062
4.579ValGlu: 4.579 ± 0.07
2.864ValPhe: 2.864 ± 0.05
4.942ValGly: 4.942 ± 0.07
1.317ValHis: 1.317 ± 0.033
3.89ValIle: 3.89 ± 0.066
2.781ValLys: 2.781 ± 0.058
7.213ValLeu: 7.213 ± 0.082
1.541ValMet: 1.541 ± 0.034
1.999ValAsn: 1.999 ± 0.045
3.794ValPro: 3.794 ± 0.063
1.98ValGln: 1.98 ± 0.041
4.902ValArg: 4.902 ± 0.066
4.489ValSer: 4.489 ± 0.068
4.092ValThr: 4.092 ± 0.06
5.442ValVal: 5.442 ± 0.077
0.88ValTrp: 0.88 ± 0.029
1.605ValTyr: 1.605 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.144TrpAla: 1.144 ± 0.034
0.123TrpCys: 0.123 ± 0.01
0.592TrpAsp: 0.592 ± 0.023
0.543TrpGlu: 0.543 ± 0.024
0.496TrpPhe: 0.496 ± 0.022
0.9TrpGly: 0.9 ± 0.031
0.299TrpHis: 0.299 ± 0.017
0.581TrpIle: 0.581 ± 0.023
0.484TrpLys: 0.484 ± 0.024
1.617TrpLeu: 1.617 ± 0.04
0.276TrpMet: 0.276 ± 0.015
0.402TrpAsn: 0.402 ± 0.022
0.637TrpPro: 0.637 ± 0.026
0.552TrpGln: 0.552 ± 0.021
1.212TrpArg: 1.212 ± 0.039
0.73TrpSer: 0.73 ± 0.03
0.752TrpThr: 0.752 ± 0.026
0.72TrpVal: 0.72 ± 0.025
0.185TrpTrp: 0.185 ± 0.013
0.282TrpTyr: 0.282 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.581TyrAla: 2.581 ± 0.047
0.265TyrCys: 0.265 ± 0.018
1.45TyrAsp: 1.45 ± 0.039
1.348TyrGlu: 1.348 ± 0.036
0.989TyrPhe: 0.989 ± 0.031
2.116TyrGly: 2.116 ± 0.045
0.469TyrHis: 0.469 ± 0.018
1.026TyrIle: 1.026 ± 0.033
0.824TyrLys: 0.824 ± 0.028
2.261TyrLeu: 2.261 ± 0.046
0.415TyrMet: 0.415 ± 0.019
0.704TyrAsn: 0.704 ± 0.028
1.04TyrPro: 1.04 ± 0.033
0.738TyrGln: 0.738 ± 0.024
1.831TyrArg: 1.831 ± 0.04
1.256TyrSer: 1.256 ± 0.036
1.148TyrThr: 1.148 ± 0.036
1.633TyrVal: 1.633 ± 0.044
0.367TyrTrp: 0.367 ± 0.019
0.696TyrTyr: 0.696 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3513 proteins (1117615 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski