Tomato aspermy virus (TAV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Cucumovirus

Average proteome isoelectric point is 7.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q88619|Q88619_TAV Capsid protein OS=Tomato aspermy virus OX=12315 GN=cp PE=2 SV=1
MM1 pKa = 7.19STFSLEE7 pKa = 3.74NCLNGSYY14 pKa = 10.84GVDD17 pKa = 3.2TPEE20 pKa = 3.55EE21 pKa = 3.98VTFIRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84DD29 pKa = 3.63ANPSAFHH36 pKa = 6.99GEE38 pKa = 3.94EE39 pKa = 4.11TVPSNTTEE47 pKa = 3.87VHH49 pKa = 5.87SEE51 pKa = 4.17SVSYY55 pKa = 11.13SSDD58 pKa = 3.46FEE60 pKa = 5.35GYY62 pKa = 10.22SCDD65 pKa = 4.68SDD67 pKa = 3.63FTGDD71 pKa = 3.41NQYY74 pKa = 11.51SSFDD78 pKa = 3.65GADD81 pKa = 3.71SLLLSYY87 pKa = 10.79DD88 pKa = 2.79IDD90 pKa = 3.92EE91 pKa = 5.26YY92 pKa = 11.13IDD94 pKa = 3.86LSEE97 pKa = 4.89SEE99 pKa = 4.47LRR101 pKa = 11.84VLFDD105 pKa = 4.15EE106 pKa = 4.8MVQPIHH112 pKa = 6.84FGMVLSPTFSRR123 pKa = 11.84NRR125 pKa = 11.84FLSCLSLAKK134 pKa = 10.16SVVVAPLYY142 pKa = 10.84DD143 pKa = 3.45PEE145 pKa = 4.22RR146 pKa = 11.84TLRR149 pKa = 11.84PFPEE153 pKa = 4.24LVKK156 pKa = 10.6NLYY159 pKa = 10.62SGVHH163 pKa = 7.03DD164 pKa = 4.7IYY166 pKa = 11.3LSDD169 pKa = 3.92SEE171 pKa = 4.31EE172 pKa = 3.92AAISSVTDD180 pKa = 3.79TIEE183 pKa = 4.74GYY185 pKa = 8.49TFSPRR190 pKa = 11.84DD191 pKa = 3.3VVDD194 pKa = 3.72NYY196 pKa = 10.63EE197 pKa = 4.4PPPLCSVCGLIAYY210 pKa = 7.44QCPHH214 pKa = 6.94YY215 pKa = 10.52DD216 pKa = 3.24INSLRR221 pKa = 11.84KK222 pKa = 8.83NCAEE226 pKa = 3.69MTIAHH231 pKa = 7.57DD232 pKa = 3.93YY233 pKa = 10.26PIEE236 pKa = 4.22GLCGLIDD243 pKa = 5.32DD244 pKa = 4.6ATLLKK249 pKa = 10.91NLGSFLLPIQCEE261 pKa = 4.31YY262 pKa = 10.94TKK264 pKa = 10.9LPEE267 pKa = 4.32PTLITPPEE275 pKa = 4.12LTRR278 pKa = 11.84PTDD281 pKa = 3.25RR282 pKa = 11.84VTVDD286 pKa = 4.22LLQAICDD293 pKa = 3.95STLPTHH299 pKa = 6.17VCYY302 pKa = 10.56DD303 pKa = 3.57DD304 pKa = 5.03TYY306 pKa = 10.44HH307 pKa = 5.85QTFIEE312 pKa = 4.24NADD315 pKa = 3.46YY316 pKa = 11.16SVDD319 pKa = 3.3IDD321 pKa = 4.06RR322 pKa = 11.84VRR324 pKa = 11.84LKK326 pKa = 10.83QSDD329 pKa = 4.17LLAKK333 pKa = 10.62VIDD336 pKa = 4.21EE337 pKa = 4.33GHH339 pKa = 7.07LKK341 pKa = 10.23PVLNTGSGQKK351 pKa = 10.37RR352 pKa = 11.84IGTTRR357 pKa = 11.84EE358 pKa = 3.64VLCAIKK364 pKa = 10.17KK365 pKa = 10.03RR366 pKa = 11.84NADD369 pKa = 3.51VPEE372 pKa = 4.28LCGSVNLKK380 pKa = 10.34RR381 pKa = 11.84LSDD384 pKa = 3.91DD385 pKa = 3.37VAEE388 pKa = 4.51CFMLSFMNGDD398 pKa = 4.12KK399 pKa = 10.85LCSSNFINIVSDD411 pKa = 3.79FHH413 pKa = 9.25AYY415 pKa = 7.45MSKK418 pKa = 8.18WQSVLSYY425 pKa = 11.52DD426 pKa = 5.54DD427 pKa = 5.9LPDD430 pKa = 4.0LNAEE434 pKa = 3.83NLQFYY439 pKa = 9.9EE440 pKa = 4.57HH441 pKa = 6.27MVKK444 pKa = 10.41SDD446 pKa = 3.47VKK448 pKa = 10.83PSVTDD453 pKa = 3.07TLNIDD458 pKa = 3.66RR459 pKa = 11.84PLPATITFHH468 pKa = 7.31RR469 pKa = 11.84KK470 pKa = 8.36QLTSQFSPLFTALFQRR486 pKa = 11.84FQRR489 pKa = 11.84CLTKK493 pKa = 10.46RR494 pKa = 11.84VILPVGKK501 pKa = 10.07ISSLEE506 pKa = 3.83IKK508 pKa = 10.53DD509 pKa = 3.84FSVLNKK515 pKa = 9.82FCLEE519 pKa = 3.67IDD521 pKa = 3.44LSKK524 pKa = 10.66FDD526 pKa = 3.77KK527 pKa = 11.17SQGEE531 pKa = 4.26LHH533 pKa = 7.14LMIQEE538 pKa = 5.14GILNRR543 pKa = 11.84LGCPVHH549 pKa = 7.19ISKK552 pKa = 8.59WWCDD556 pKa = 4.24FHH558 pKa = 8.96RR559 pKa = 11.84MSYY562 pKa = 10.37IKK564 pKa = 10.2DD565 pKa = 3.1KK566 pKa = 10.84RR567 pKa = 11.84AGVSMPISFQRR578 pKa = 11.84RR579 pKa = 11.84TGDD582 pKa = 2.78AFTYY586 pKa = 10.33FGNTLVTMSMFAWCYY601 pKa = 8.26DD602 pKa = 3.0TSQFDD607 pKa = 3.68KK608 pKa = 11.33LIFSGDD614 pKa = 3.51DD615 pKa = 3.19SLGFSIKK622 pKa = 10.7APVGDD627 pKa = 4.02PSLFTSLFNMEE638 pKa = 4.61AKK640 pKa = 10.52VMEE643 pKa = 4.39PSVPYY648 pKa = 10.05ICSKK652 pKa = 10.76FLLTDD657 pKa = 3.47DD658 pKa = 4.79FGNTFSVPDD667 pKa = 4.45PLRR670 pKa = 11.84EE671 pKa = 3.88IQRR674 pKa = 11.84LGSKK678 pKa = 10.16KK679 pKa = 10.13IPLDD683 pKa = 3.6EE684 pKa = 5.73DD685 pKa = 3.72DD686 pKa = 5.28RR687 pKa = 11.84SLHH690 pKa = 5.02AHH692 pKa = 6.06FMSFVDD698 pKa = 3.91RR699 pKa = 11.84LKK701 pKa = 10.95FLNHH705 pKa = 6.01MNQTSMTQLSLFYY718 pKa = 10.42EE719 pKa = 4.17MKK721 pKa = 10.12YY722 pKa = 10.38RR723 pKa = 11.84KK724 pKa = 10.14SGDD727 pKa = 4.06DD728 pKa = 3.06ILLVLGAFNKK738 pKa = 8.67YY739 pKa = 6.24TANFNAYY746 pKa = 9.22KK747 pKa = 10.1EE748 pKa = 4.42LYY750 pKa = 10.0YY751 pKa = 10.4SEE753 pKa = 4.76KK754 pKa = 9.84QQCALINSFSISDD767 pKa = 4.44LIVGRR772 pKa = 11.84GKK774 pKa = 10.36SSKK777 pKa = 9.99ASRR780 pKa = 11.84RR781 pKa = 11.84KK782 pKa = 9.69AVEE785 pKa = 4.0SNGKK789 pKa = 8.84HH790 pKa = 6.11RR791 pKa = 11.84DD792 pKa = 3.1PSTRR796 pKa = 11.84DD797 pKa = 3.17HH798 pKa = 6.92SKK800 pKa = 10.99VGTDD804 pKa = 3.38EE805 pKa = 4.46SKK807 pKa = 9.48EE808 pKa = 4.23TSTEE812 pKa = 3.76EE813 pKa = 3.99TTQTEE818 pKa = 4.23PQGAGSQKK826 pKa = 10.86SKK828 pKa = 11.24

Molecular weight: 93.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P28931|1A_TAV Replication protein 1a OS=Tomato aspermy virus OX=12315 GN=ORF1a PE=3 SV=1
MM1 pKa = 7.26AQNGTGGGSRR11 pKa = 11.84RR12 pKa = 11.84PRR14 pKa = 11.84RR15 pKa = 11.84GRR17 pKa = 11.84RR18 pKa = 11.84NNNNNNSTARR28 pKa = 11.84DD29 pKa = 3.58KK30 pKa = 11.54ALLALTQQVNRR41 pKa = 11.84LANIASSSAPSLQHH55 pKa = 5.37PTFIASKK62 pKa = 9.33KK63 pKa = 9.13CRR65 pKa = 11.84AGYY68 pKa = 8.61TYY70 pKa = 10.49TSLDD74 pKa = 3.31VRR76 pKa = 11.84PTRR79 pKa = 11.84TEE81 pKa = 3.58KK82 pKa = 10.9DD83 pKa = 2.95KK84 pKa = 11.8SFGQRR89 pKa = 11.84LIIPVPVSEE98 pKa = 4.23YY99 pKa = 9.43PKK101 pKa = 10.66KK102 pKa = 10.4KK103 pKa = 9.72VSCVQVRR110 pKa = 11.84LNPSPKK116 pKa = 9.47FNSTIWVSLRR126 pKa = 11.84RR127 pKa = 11.84LDD129 pKa = 3.62EE130 pKa = 4.33TTLLTSEE137 pKa = 4.43NVFKK141 pKa = 11.33LFTDD145 pKa = 4.24GLAAVLIYY153 pKa = 9.4QHH155 pKa = 6.54VPTGIQPNNKK165 pKa = 8.16ITFDD169 pKa = 3.57MSNVGAEE176 pKa = 3.78IGDD179 pKa = 3.54MGKK182 pKa = 10.16YY183 pKa = 10.55ALIVYY188 pKa = 10.32SKK190 pKa = 11.29DD191 pKa = 3.38DD192 pKa = 3.49VLEE195 pKa = 4.46ADD197 pKa = 4.13EE198 pKa = 4.49MVIHH202 pKa = 7.17IDD204 pKa = 3.74IEE206 pKa = 4.31HH207 pKa = 5.69QRR209 pKa = 11.84IPSLQRR215 pKa = 11.84SRR217 pKa = 11.84CDD219 pKa = 3.05STRR222 pKa = 11.84MHH224 pKa = 7.06DD225 pKa = 3.35VRR227 pKa = 11.84RR228 pKa = 11.84RR229 pKa = 3.31

Molecular weight: 25.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2920

95

993

417.1

46.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.438 ± 0.771

2.397 ± 0.2

6.61 ± 0.685

5.205 ± 0.34

4.041 ± 0.564

5.137 ± 0.277

2.637 ± 0.247

5.034 ± 0.261

5.685 ± 0.542

9.178 ± 0.324

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.466 ± 0.205

4.178 ± 0.597

4.486 ± 0.642

3.699 ± 0.315

6.233 ± 0.74

9.075 ± 0.807

6.712 ± 0.505

6.849 ± 0.488

0.856 ± 0.162

3.082 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski