Mycoplasma hyorhinis SK76

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma hyorhinis

Average proteome isoelectric point is 7.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 751 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7XKN1|K7XKN1_MYCHR Thioredoxin domain-containing protein OS=Mycoplasma hyorhinis SK76 OX=1118964 GN=MOS_100 PE=4 SV=1
MM1 pKa = 7.32FSSIGLFLSIEE12 pKa = 3.99AKK14 pKa = 10.79GIDD17 pKa = 3.39VCEE20 pKa = 4.21IEE22 pKa = 5.05LEE24 pKa = 4.09IGFSISFCIFSVLFCEE40 pKa = 5.15ISTSYY45 pKa = 10.75LFKK48 pKa = 10.78LIVSFEE54 pKa = 4.1IVSVFVLIFITSSLFLSSFVLVSTKK79 pKa = 10.27EE80 pKa = 3.37IWFLFGSVKK89 pKa = 9.12ITFLLVFSSFWLGFSWISSWDD110 pKa = 3.4LVLFSFFEE118 pKa = 4.05KK119 pKa = 10.37MNLFDD124 pKa = 5.79YY125 pKa = 10.25VKK127 pKa = 10.64NWNLCFLNEE136 pKa = 3.99YY137 pKa = 10.65LFLDD141 pKa = 4.15DD142 pKa = 3.44QTFYY146 pKa = 11.73

Molecular weight: 17.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7X245|K7X245_MYCHR DNA topoisomerase (ATP-hydrolyzing) OS=Mycoplasma hyorhinis SK76 OX=1118964 GN=MOS_143 PE=4 SV=1
MM1 pKa = 7.88ADD3 pKa = 3.09KK4 pKa = 10.78KK5 pKa = 9.68ITYY8 pKa = 9.61RR9 pKa = 11.84GLGRR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 9.81SSVARR20 pKa = 11.84VIITPGTGEE29 pKa = 3.81FKK31 pKa = 10.46INKK34 pKa = 8.94RR35 pKa = 11.84AAKK38 pKa = 10.15DD39 pKa = 3.66YY40 pKa = 11.41LKK42 pKa = 10.8LDD44 pKa = 4.31LLIQDD49 pKa = 4.74ALQPLEE55 pKa = 4.03ITKK58 pKa = 7.96TTDD61 pKa = 2.33QWNISVNASGGGLSGQAGAIRR82 pKa = 11.84LGIARR87 pKa = 11.84ALLEE91 pKa = 4.0ASADD95 pKa = 3.56YY96 pKa = 10.8RR97 pKa = 11.84PEE99 pKa = 4.19LKK101 pKa = 10.49SKK103 pKa = 10.6GMLTRR108 pKa = 11.84DD109 pKa = 3.06ARR111 pKa = 11.84AKK113 pKa = 9.34EE114 pKa = 3.95RR115 pKa = 11.84KK116 pKa = 9.7KK117 pKa = 10.8FGLRR121 pKa = 11.84AARR124 pKa = 11.84RR125 pKa = 11.84SRR127 pKa = 11.84QFSKK131 pKa = 10.94RR132 pKa = 3.36

Molecular weight: 14.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

751

0

751

250504

50

3704

333.6

38.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.037 ± 0.09

0.391 ± 0.031

5.051 ± 0.079

6.51 ± 0.104

6.297 ± 0.117

4.174 ± 0.085

1.432 ± 0.033

9.236 ± 0.117

10.729 ± 0.112

9.774 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.374 ± 0.035

7.677 ± 0.111

2.601 ± 0.045

4.294 ± 0.068

2.706 ± 0.055

7.23 ± 0.094

5.234 ± 0.122

5.394 ± 0.074

1.004 ± 0.032

3.856 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski