Shahe hepe-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KK16|A0A1L3KK16_9VIRU Uncharacterized protein OS=Shahe hepe-like virus 1 OX=1923415 PE=4 SV=1
MM1 pKa = 7.42NSDD4 pKa = 3.66PAQANSVSEE13 pKa = 4.1GAPVEE18 pKa = 4.17LSTGSTGLPSSDD30 pKa = 3.37FTDD33 pKa = 3.5SKK35 pKa = 11.33PLFLEE40 pKa = 5.07AGTQHH45 pKa = 6.17TAVHH49 pKa = 6.76CFRR52 pKa = 11.84VAKK55 pKa = 10.58DD56 pKa = 3.64LVTLAEE62 pKa = 4.38GSKK65 pKa = 10.31PGEE68 pKa = 3.83ICFVQPLTPFGIDD81 pKa = 3.03NTATNLASDD90 pKa = 3.75YY91 pKa = 10.75EE92 pKa = 4.56YY93 pKa = 11.6YY94 pKa = 10.66SFKK97 pKa = 10.77NVEE100 pKa = 3.82IAIEE104 pKa = 4.08AVSPFGTSSGGMQVAWITDD123 pKa = 3.85PEE125 pKa = 4.58NVTLPQGDD133 pKa = 4.54DD134 pKa = 3.75GQKK137 pKa = 8.95LTSLAKK143 pKa = 10.33VIRR146 pKa = 11.84QDD148 pKa = 2.89GSVLVRR154 pKa = 11.84PRR156 pKa = 11.84NSALFNISTEE166 pKa = 4.05GARR169 pKa = 11.84YY170 pKa = 8.94CLPGTEE176 pKa = 4.53SRR178 pKa = 11.84LSSFGSLVALVRR190 pKa = 11.84SPPGTGDD197 pKa = 3.31VADD200 pKa = 5.37FAATITGEE208 pKa = 3.85IHH210 pKa = 7.04FMRR213 pKa = 11.84STRR216 pKa = 11.84NAPVYY221 pKa = 10.25IGEE224 pKa = 4.22FPIGTFTIDD233 pKa = 3.08EE234 pKa = 4.36TQPLIEE240 pKa = 4.84EE241 pKa = 4.46NDD243 pKa = 4.08LILPVRR249 pKa = 11.84LSSNDD254 pKa = 3.09VQTDD258 pKa = 2.89ISRR261 pKa = 11.84WDD263 pKa = 3.2FRR265 pKa = 11.84TPLKK269 pKa = 9.99LTLRR273 pKa = 11.84GKK275 pKa = 10.21DD276 pKa = 3.37GRR278 pKa = 11.84FTKK281 pKa = 10.04IHH283 pKa = 6.58EE284 pKa = 4.36IKK286 pKa = 10.8ADD288 pKa = 3.55FATFKK293 pKa = 9.93LTNIDD298 pKa = 3.29DD299 pKa = 3.96QYY301 pKa = 12.05AEE303 pKa = 5.13GILMIDD309 pKa = 3.78LPFYY313 pKa = 11.1GLTNPVVRR321 pKa = 11.84INSFPRR327 pKa = 11.84QIINSYY333 pKa = 10.07EE334 pKa = 3.92VLRR337 pKa = 11.84NN338 pKa = 3.42

Molecular weight: 36.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KK10|A0A1L3KK10_9VIRU Putative structural protein OS=Shahe hepe-like virus 1 OX=1923415 PE=4 SV=1
MM1 pKa = 7.11QVRR4 pKa = 11.84ILTQYY9 pKa = 10.26QRR11 pKa = 11.84EE12 pKa = 4.02QRR14 pKa = 11.84KK15 pKa = 9.84DD16 pKa = 3.08RR17 pKa = 11.84VKK19 pKa = 11.24YY20 pKa = 10.45LLQTFKK26 pKa = 11.17LSLSKK31 pKa = 10.92VPKK34 pKa = 9.43TDD36 pKa = 3.7LFRR39 pKa = 11.84QINTIISNQFKK50 pKa = 9.59MRR52 pKa = 11.84PLMPVPFSVTPPKK65 pKa = 10.05QASHH69 pKa = 6.94SIHH72 pKa = 6.65HH73 pKa = 5.81SRR75 pKa = 11.84VRR77 pKa = 11.84NTGLAKK83 pKa = 10.96LITNGKK89 pKa = 7.97NDD91 pKa = 3.47EE92 pKa = 4.5FEE94 pKa = 4.16FHH96 pKa = 7.12LLDD99 pKa = 4.93AEE101 pKa = 4.17NKK103 pKa = 10.19YY104 pKa = 10.85FFDD107 pKa = 3.91EE108 pKa = 4.24NALEE112 pKa = 3.97MFKK115 pKa = 10.49NHH117 pKa = 5.42EE118 pKa = 4.17YY119 pKa = 11.07YY120 pKa = 10.31RR121 pKa = 11.84IKK123 pKa = 10.3EE124 pKa = 3.57ITMTINNTEE133 pKa = 3.33NWLKK137 pKa = 10.75VRR139 pKa = 11.84GAFQASYY146 pKa = 10.57VHH148 pKa = 6.98DD149 pKa = 4.23PEE151 pKa = 6.31GRR153 pKa = 11.84ADD155 pKa = 3.71PLSVRR160 pKa = 11.84FQDD163 pKa = 3.62TQLVRR168 pKa = 11.84IGKK171 pKa = 9.37AVVWQIPVLKK181 pKa = 9.68KK182 pKa = 9.67WLYY185 pKa = 5.22TTSGGSSGSKK195 pKa = 9.76RR196 pKa = 11.84LSAAGKK202 pKa = 9.43VIIKK206 pKa = 9.92IRR208 pKa = 11.84SKK210 pKa = 10.86QNNVKK215 pKa = 10.29DD216 pKa = 3.92APPNGAEE223 pKa = 4.14SYY225 pKa = 10.2KK226 pKa = 10.72VAVTIDD232 pKa = 3.99AIIEE236 pKa = 4.36FCSKK240 pKa = 9.23TRR242 pKa = 11.84ILRR245 pKa = 11.84LGGIIYY251 pKa = 8.79RR252 pKa = 11.84IPFTGDD258 pKa = 2.37AGGQVVNDD266 pKa = 3.51YY267 pKa = 10.94VRR269 pKa = 11.84LFTKK273 pKa = 10.29TDD275 pKa = 3.17DD276 pKa = 3.39RR277 pKa = 11.84DD278 pKa = 3.61YY279 pKa = 11.65YY280 pKa = 11.24LDD282 pKa = 3.35IRR284 pKa = 11.84IAEE287 pKa = 4.59LFPFKK292 pKa = 10.07PAQGFFFLQSPVMVTFKK309 pKa = 10.43FHH311 pKa = 7.76SSEE314 pKa = 4.06EE315 pKa = 4.18TNKK318 pKa = 10.38IIIYY322 pKa = 10.36DD323 pKa = 3.77FMMTNGNFYY332 pKa = 10.42MNEE335 pKa = 3.92DD336 pKa = 3.98SLSIWLRR343 pKa = 11.84TKK345 pKa = 10.69VDD347 pKa = 2.72IGSAYY352 pKa = 10.54SGTDD356 pKa = 3.48FQPDD360 pKa = 3.24EE361 pKa = 4.44LSIEE365 pKa = 4.09FQYY368 pKa = 11.57DD369 pKa = 3.09SAYY372 pKa = 10.57LEE374 pKa = 4.0QGIIPNFITFMPDD387 pKa = 2.82DD388 pKa = 3.92FEE390 pKa = 4.99KK391 pKa = 10.96EE392 pKa = 3.87KK393 pKa = 10.42TFSYY397 pKa = 10.42RR398 pKa = 11.84DD399 pKa = 3.2QRR401 pKa = 11.84PRR403 pKa = 11.84KK404 pKa = 9.01FLQQ407 pKa = 3.4

Molecular weight: 47.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3206

173

2103

641.2

72.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.174 ± 0.938

1.372 ± 0.514

5.646 ± 0.309

6.02 ± 0.425

5.552 ± 0.7

5.209 ± 0.518

2.277 ± 0.589

7.143 ± 0.468

7.049 ± 0.957

8.141 ± 0.599

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.183 ± 0.314

5.739 ± 0.411

4.46 ± 0.502

3.587 ± 0.619

4.523 ± 0.446

6.644 ± 0.632

7.299 ± 0.74

5.77 ± 0.437

0.967 ± 0.227

3.244 ± 0.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski