Firmicutes bacterium R50

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; unclassified Firmicutes sensu stricto

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2747 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6F8ZJ71|A0A6F8ZJ71_9FIRM HTH_17 domain-containing protein OS=Firmicutes bacterium R50 OX=2707344 GN=R50_2547 PE=4 SV=1
MM1 pKa = 7.52PACLVCGEE9 pKa = 4.95PGDD12 pKa = 4.08PDD14 pKa = 4.39RR15 pKa = 11.84DD16 pKa = 3.38FALYY20 pKa = 8.48LTAEE24 pKa = 4.28SGDD27 pKa = 3.64PVSASFCSEE36 pKa = 3.62ACEE39 pKa = 4.09DD40 pKa = 3.96QAVLDD45 pKa = 4.53APPLVATCDD54 pKa = 3.03RR55 pKa = 11.84CGRR58 pKa = 11.84DD59 pKa = 3.77LLVAMDD65 pKa = 4.64GVDD68 pKa = 3.88LRR70 pKa = 11.84IPQAYY75 pKa = 9.38NVLTNGDD82 pKa = 3.84TGEE85 pKa = 4.22VLCLRR90 pKa = 11.84CAGVDD95 pKa = 3.54RR96 pKa = 11.84EE97 pKa = 4.31RR98 pKa = 11.84LGDD101 pKa = 4.61LADD104 pKa = 3.82PWEE107 pKa = 4.56LGAA110 pKa = 6.07

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6F8ZD39|A0A6F8ZD39_9FIRM Uncharacterized protein OS=Firmicutes bacterium R50 OX=2707344 GN=R50_0282 PE=4 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84LALRR6 pKa = 11.84RR7 pKa = 11.84AARR10 pKa = 11.84ISPASAFSSNSGPAVIQNANLAMPTPHH37 pKa = 6.89FLSGIRR43 pKa = 11.84IRR45 pKa = 4.61

Molecular weight: 4.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2747

0

2747

771356

20

1439

280.8

30.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.139 ± 0.079

0.621 ± 0.017

4.358 ± 0.041

5.534 ± 0.061

2.802 ± 0.026

9.875 ± 0.056

2.044 ± 0.023

3.179 ± 0.04

1.491 ± 0.032

11.627 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.875 ± 0.021

1.643 ± 0.024

7.04 ± 0.054

3.019 ± 0.034

9.109 ± 0.059

3.92 ± 0.036

4.808 ± 0.037

8.503 ± 0.039

2.239 ± 0.029

2.174 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski