Maize rayado fino virus (isolate Costa Rica/Guapiles) (MRFV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Tymoviridae; Marafivirus; Maize rayado fino virus

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q91TW9-2|POLG-2_MRFVC Isoform of Q91TW9 Isoform Subgenomic capsid protein CP2 of Genome polyprotein OS=Maize rayado fino virus (isolate Costa Rica/Guapiles) OX=652669 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.98ADD3 pKa = 3.15NATQVGPVPPRR14 pKa = 11.84DD15 pKa = 3.61DD16 pKa = 3.59RR17 pKa = 11.84VDD19 pKa = 3.74RR20 pKa = 11.84QPPLPDD26 pKa = 3.57PPRR29 pKa = 11.84VLEE32 pKa = 4.02TAPSHH37 pKa = 7.1FLDD40 pKa = 6.1LPFQWKK46 pKa = 7.96VTDD49 pKa = 3.78FTGYY53 pKa = 10.43AAYY56 pKa = 10.18HH57 pKa = 5.47GTDD60 pKa = 3.96DD61 pKa = 4.23LVASAVLTTLCAPYY75 pKa = 10.22RR76 pKa = 11.84HH77 pKa = 6.64AEE79 pKa = 3.94LLYY82 pKa = 10.82VEE84 pKa = 5.48ISVAPCPPSFSKK96 pKa = 10.86PIMFTVVWTPATLSPRR112 pKa = 11.84DD113 pKa = 3.72GKK115 pKa = 9.3EE116 pKa = 3.21TDD118 pKa = 3.7YY119 pKa = 11.7YY120 pKa = 10.94GGRR123 pKa = 11.84QITVGGPVMLSSTTAVPADD142 pKa = 4.22LARR145 pKa = 11.84MNPFIKK151 pKa = 10.54SSVSYY156 pKa = 10.97NDD158 pKa = 3.26TPRR161 pKa = 11.84WTMSVPAVTGGDD173 pKa = 3.51TKK175 pKa = 10.95IPLATAFVRR184 pKa = 11.84GIVRR188 pKa = 11.84VRR190 pKa = 11.84APSGAATPSAA200 pKa = 3.93

Molecular weight: 21.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q91TW9|POLG_MRFVC Genome polyprotein OS=Maize rayado fino virus (isolate Costa Rica/Guapiles) OX=652669 GN=ORF1 PE=4 SV=1
MM1 pKa = 8.0PLTPTPSIRR10 pKa = 11.84PSRR13 pKa = 11.84PTSFSMSGPTTLGVRR28 pKa = 11.84QTSCSSSLRR37 pKa = 11.84SSPSSSPDD45 pKa = 3.09SPTSPTSSTTGSCPKK60 pKa = 9.29TPPGTPPLPRR70 pKa = 11.84TSRR73 pKa = 11.84TARR76 pKa = 11.84PSSCMMLSCITPQGRR91 pKa = 11.84SLTSSSSVPSSRR103 pKa = 11.84RR104 pKa = 11.84SMPPLSFRR112 pKa = 11.84LSRR115 pKa = 11.84ASRR118 pKa = 11.84ISRR121 pKa = 11.84SIRR124 pKa = 11.84KK125 pKa = 7.44FTASVFRR132 pKa = 11.84VLTLSTSRR140 pKa = 11.84RR141 pKa = 11.84ATQPPTTRR149 pKa = 11.84SRR151 pKa = 11.84VRR153 pKa = 11.84PSTGSRR159 pKa = 11.84PPVSPLVTSSSPSPFSTLSARR180 pKa = 11.84SIPSSSSVAALPSFRR195 pKa = 11.84PKK197 pKa = 10.09TLLPSAFRR205 pKa = 11.84TPSPSPLPPPSTRR218 pKa = 11.84TFVTGWSPARR228 pKa = 11.84CTTRR232 pKa = 11.84SSIMSEE238 pKa = 3.86PSAFGLPTPPASSGLRR254 pKa = 11.84SASLSTAGSPLPPGTTFSTSRR275 pKa = 11.84SRR277 pKa = 11.84PPPFVPTPRR286 pKa = 11.84IPFSSRR292 pKa = 11.84PSLACPTGFAPTLGRR307 pKa = 11.84SGAWLPPRR315 pKa = 11.84PLSLPGPLPVPSAGSSPFTPTVSGCSASTSSAGGSGLVSHH355 pKa = 6.8SMAPSQGFFGRR366 pKa = 11.84PIQPAARR373 pKa = 11.84QCSLPTPPLSAKK385 pKa = 10.39SLLVWQTGVLPPPSGRR401 pKa = 11.84VCSPRR406 pKa = 11.84PLRR409 pKa = 11.84RR410 pKa = 11.84PGSPTQPWPP419 pKa = 3.27

Molecular weight: 43.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

1

3

2646

200

2027

882.0

96.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.788 ± 1.458

1.474 ± 0.206

4.875 ± 1.467

3.175 ± 1.185

4.724 ± 0.497

5.329 ± 0.319

2.91 ± 1.125

2.91 ± 0.347

2.192 ± 0.41

9.864 ± 1.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.55 ± 0.216

1.587 ± 0.574

11.489 ± 2.816

2.683 ± 0.367

7.596 ± 0.666

10.847 ± 3.935

6.916 ± 1.848

6.16 ± 0.777

1.512 ± 0.199

2.419 ± 0.767
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski