Aciduliprofundum boonei (strain DSM 19572 / T469)

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; DHVE2 group; Aciduliprofundum; Aciduliprofundum boonei

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1539 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5IAI9|B5IAI9_ACIB4 Oligopeptide/dipeptide ABC transporter ATPase subunit OS=Aciduliprofundum boonei (strain DSM 19572 / T469) OX=439481 GN=Aboo_0845 PE=4 SV=1
MM1 pKa = 7.75KK2 pKa = 9.29WRR4 pKa = 11.84CMVCGYY10 pKa = 10.28IYY12 pKa = 10.47DD13 pKa = 4.35PEE15 pKa = 5.13VGDD18 pKa = 4.38PDD20 pKa = 3.9SGVPAGTSFEE30 pKa = 4.41EE31 pKa = 4.92LPEE34 pKa = 4.54DD35 pKa = 3.78WVCPVCGASKK45 pKa = 11.33DD46 pKa = 3.57MFEE49 pKa = 4.6KK50 pKa = 11.16VEE52 pKa = 3.95

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5IFD6|B5IFD6_ACIB4 DNA-binding protein Aboo_0025 OS=Aciduliprofundum boonei (strain DSM 19572 / T469) OX=439481 GN=Aboo_0025 PE=3 SV=1
MM1 pKa = 7.56ARR3 pKa = 11.84NKK5 pKa = 10.07HH6 pKa = 4.95VARR9 pKa = 11.84KK10 pKa = 8.17MRR12 pKa = 11.84LIRR15 pKa = 11.84KK16 pKa = 6.88MNSNRR21 pKa = 11.84RR22 pKa = 11.84VPAWVMMRR30 pKa = 11.84TDD32 pKa = 3.4RR33 pKa = 11.84KK34 pKa = 8.91FTQHH38 pKa = 4.32PHH40 pKa = 4.81RR41 pKa = 11.84RR42 pKa = 11.84NWRR45 pKa = 11.84RR46 pKa = 11.84NKK48 pKa = 10.66LKK50 pKa = 10.85VV51 pKa = 3.09

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1539

0

1539

467080

33

2166

303.5

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.392 ± 0.059

0.693 ± 0.021

5.06 ± 0.05

7.858 ± 0.104

4.24 ± 0.053

7.054 ± 0.058

1.75 ± 0.023

9.318 ± 0.07

8.267 ± 0.09

9.062 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.031

4.59 ± 0.094

3.81 ± 0.04

1.836 ± 0.029

4.787 ± 0.065

5.442 ± 0.073

4.279 ± 0.061

6.905 ± 0.054

1.169 ± 0.029

4.604 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski