Amino acid dipepetide frequency for Enterobacteria phage mEp460

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.836AlaAla: 13.836 ± 1.995
1.087AlaCys: 1.087 ± 0.382
6.012AlaAsp: 6.012 ± 0.705
5.288AlaGlu: 5.288 ± 0.623
3.694AlaPhe: 3.694 ± 0.576
8.548AlaGly: 8.548 ± 0.924
1.449AlaHis: 1.449 ± 0.306
4.419AlaIle: 4.419 ± 0.568
4.708AlaLys: 4.708 ± 0.904
8.475AlaLeu: 8.475 ± 0.773
3.405AlaMet: 3.405 ± 0.56
3.115AlaAsn: 3.115 ± 0.548
2.898AlaPro: 2.898 ± 0.533
3.839AlaGln: 3.839 ± 0.683
5.723AlaArg: 5.723 ± 0.759
7.389AlaSer: 7.389 ± 1.09
5.723AlaThr: 5.723 ± 0.874
6.954AlaVal: 6.954 ± 0.917
1.594AlaTrp: 1.594 ± 0.351
2.825AlaTyr: 2.825 ± 0.444
0.0AlaXaa: 0.0 ± 0.0
Cys
1.159CysAla: 1.159 ± 0.467
0.29CysCys: 0.29 ± 0.15
0.58CysAsp: 0.58 ± 0.199
0.724CysGlu: 0.724 ± 0.286
0.362CysPhe: 0.362 ± 0.159
1.304CysGly: 1.304 ± 0.395
0.145CysHis: 0.145 ± 0.098
0.507CysIle: 0.507 ± 0.151
0.507CysLys: 0.507 ± 0.228
1.014CysLeu: 1.014 ± 0.282
0.362CysMet: 0.362 ± 0.144
0.58CysAsn: 0.58 ± 0.186
0.724CysPro: 0.724 ± 0.286
0.724CysGln: 0.724 ± 0.276
1.159CysArg: 1.159 ± 0.468
1.087CysSer: 1.087 ± 0.374
0.797CysThr: 0.797 ± 0.27
0.724CysVal: 0.724 ± 0.288
0.362CysTrp: 0.362 ± 0.214
0.435CysTyr: 0.435 ± 0.177
0.0CysXaa: 0.0 ± 0.0
Asp
6.302AspAla: 6.302 ± 0.604
0.724AspCys: 0.724 ± 0.208
3.984AspAsp: 3.984 ± 0.601
3.332AspGlu: 3.332 ± 0.482
1.521AspPhe: 1.521 ± 0.387
6.012AspGly: 6.012 ± 0.733
0.58AspHis: 0.58 ± 0.18
2.825AspIle: 2.825 ± 0.467
2.825AspLys: 2.825 ± 0.507
4.853AspLeu: 4.853 ± 0.717
1.304AspMet: 1.304 ± 0.282
3.477AspAsn: 3.477 ± 0.448
2.246AspPro: 2.246 ± 0.524
2.028AspGln: 2.028 ± 0.35
2.246AspArg: 2.246 ± 0.483
2.825AspSer: 2.825 ± 0.52
2.028AspThr: 2.028 ± 0.429
4.419AspVal: 4.419 ± 0.704
1.376AspTrp: 1.376 ± 0.437
1.739AspTyr: 1.739 ± 0.348
0.0AspXaa: 0.0 ± 0.0
Glu
5.216GluAla: 5.216 ± 0.553
0.797GluCys: 0.797 ± 0.247
2.825GluAsp: 2.825 ± 0.426
3.26GluGlu: 3.26 ± 0.598
1.594GluPhe: 1.594 ± 0.337
3.332GluGly: 3.332 ± 0.559
1.159GluHis: 1.159 ± 0.299
3.042GluIle: 3.042 ± 0.44
3.839GluLys: 3.839 ± 0.507
6.882GluLeu: 6.882 ± 0.798
1.449GluMet: 1.449 ± 0.392
2.535GluAsn: 2.535 ± 0.477
2.39GluPro: 2.39 ± 0.53
3.767GluGln: 3.767 ± 0.629
4.201GluArg: 4.201 ± 0.692
3.622GluSer: 3.622 ± 0.618
4.274GluThr: 4.274 ± 0.692
3.26GluVal: 3.26 ± 0.428
1.449GluTrp: 1.449 ± 0.401
1.376GluTyr: 1.376 ± 0.285
0.0GluXaa: 0.0 ± 0.0
Phe
2.318PheAla: 2.318 ± 0.473
0.652PheCys: 0.652 ± 0.274
2.246PheAsp: 2.246 ± 0.427
2.101PheGlu: 2.101 ± 0.408
1.087PhePhe: 1.087 ± 0.298
1.883PheGly: 1.883 ± 0.426
0.435PheHis: 0.435 ± 0.179
1.811PheIle: 1.811 ± 0.336
1.883PheLys: 1.883 ± 0.378
2.028PheLeu: 2.028 ± 0.435
1.449PheMet: 1.449 ± 0.287
1.521PheAsn: 1.521 ± 0.295
1.521PhePro: 1.521 ± 0.349
0.942PheGln: 0.942 ± 0.218
2.753PheArg: 2.753 ± 0.521
2.68PheSer: 2.68 ± 0.458
2.028PheThr: 2.028 ± 0.409
2.97PheVal: 2.97 ± 0.421
1.159PheTrp: 1.159 ± 0.242
1.087PheTyr: 1.087 ± 0.243
0.072PheXaa: 0.072 ± 0.071
Gly
6.447GlyAla: 6.447 ± 0.717
0.942GlyCys: 0.942 ± 0.297
4.781GlyAsp: 4.781 ± 0.886
4.491GlyGlu: 4.491 ± 0.68
3.26GlyPhe: 3.26 ± 0.567
5.216GlyGly: 5.216 ± 0.621
1.159GlyHis: 1.159 ± 0.29
3.912GlyIle: 3.912 ± 0.589
5.433GlyLys: 5.433 ± 0.649
4.201GlyLeu: 4.201 ± 0.486
2.463GlyMet: 2.463 ± 0.387
4.201GlyAsn: 4.201 ± 0.71
2.318GlyPro: 2.318 ± 0.362
3.187GlyGln: 3.187 ± 0.573
4.564GlyArg: 4.564 ± 0.573
4.853GlySer: 4.853 ± 0.607
3.839GlyThr: 3.839 ± 0.892
6.085GlyVal: 6.085 ± 0.656
1.521GlyTrp: 1.521 ± 0.301
2.246GlyTyr: 2.246 ± 0.429
0.0GlyXaa: 0.0 ± 0.0
His
1.883HisAla: 1.883 ± 0.483
0.435HisCys: 0.435 ± 0.204
1.087HisAsp: 1.087 ± 0.253
1.087HisGlu: 1.087 ± 0.362
1.014HisPhe: 1.014 ± 0.251
1.159HisGly: 1.159 ± 0.356
0.652HisHis: 0.652 ± 0.306
1.159HisIle: 1.159 ± 0.372
0.942HisLys: 0.942 ± 0.228
1.376HisLeu: 1.376 ± 0.327
0.58HisMet: 0.58 ± 0.198
0.652HisAsn: 0.652 ± 0.209
0.724HisPro: 0.724 ± 0.215
0.724HisGln: 0.724 ± 0.212
1.087HisArg: 1.087 ± 0.306
0.869HisSer: 0.869 ± 0.313
0.724HisThr: 0.724 ± 0.212
0.797HisVal: 0.797 ± 0.238
0.435HisTrp: 0.435 ± 0.176
0.58HisTyr: 0.58 ± 0.153
0.0HisXaa: 0.0 ± 0.0
Ile
5.216IleAla: 5.216 ± 0.625
1.014IleCys: 1.014 ± 0.284
3.767IleAsp: 3.767 ± 0.442
2.898IleGlu: 2.898 ± 0.592
1.376IlePhe: 1.376 ± 0.375
3.405IleGly: 3.405 ± 0.645
0.724IleHis: 0.724 ± 0.214
2.68IleIle: 2.68 ± 0.523
2.463IleLys: 2.463 ± 0.4
2.753IleLeu: 2.753 ± 0.434
0.942IleMet: 0.942 ± 0.286
3.187IleAsn: 3.187 ± 0.482
2.68IlePro: 2.68 ± 0.448
1.666IleGln: 1.666 ± 0.35
4.057IleArg: 4.057 ± 0.605
4.201IleSer: 4.201 ± 0.562
3.477IleThr: 3.477 ± 0.721
2.173IleVal: 2.173 ± 0.373
0.507IleTrp: 0.507 ± 0.199
1.014IleTyr: 1.014 ± 0.364
0.0IleXaa: 0.0 ± 0.0
Lys
5.65LysAla: 5.65 ± 0.721
1.231LysCys: 1.231 ± 0.403
2.608LysAsp: 2.608 ± 0.348
2.898LysGlu: 2.898 ± 0.474
1.739LysPhe: 1.739 ± 0.372
4.129LysGly: 4.129 ± 0.59
0.942LysHis: 0.942 ± 0.235
2.608LysIle: 2.608 ± 0.509
2.463LysLys: 2.463 ± 0.463
4.129LysLeu: 4.129 ± 0.682
1.594LysMet: 1.594 ± 0.315
2.535LysAsn: 2.535 ± 0.517
1.811LysPro: 1.811 ± 0.454
2.246LysGln: 2.246 ± 0.329
3.332LysArg: 3.332 ± 0.486
3.549LysSer: 3.549 ± 0.571
3.477LysThr: 3.477 ± 0.411
3.405LysVal: 3.405 ± 0.466
0.724LysTrp: 0.724 ± 0.207
1.811LysTyr: 1.811 ± 0.299
0.0LysXaa: 0.0 ± 0.0
Leu
7.823LeuAla: 7.823 ± 0.776
1.304LeuCys: 1.304 ± 0.421
4.419LeuAsp: 4.419 ± 0.483
3.912LeuGlu: 3.912 ± 0.541
2.39LeuPhe: 2.39 ± 0.501
4.926LeuGly: 4.926 ± 0.685
1.739LeuHis: 1.739 ± 0.386
3.839LeuIle: 3.839 ± 0.688
4.636LeuLys: 4.636 ± 0.601
4.853LeuLeu: 4.853 ± 0.537
1.739LeuMet: 1.739 ± 0.411
4.201LeuAsn: 4.201 ± 0.633
3.839LeuPro: 3.839 ± 0.7
3.332LeuGln: 3.332 ± 0.514
5.433LeuArg: 5.433 ± 0.528
6.882LeuSer: 6.882 ± 0.861
6.157LeuThr: 6.157 ± 0.709
5.505LeuVal: 5.505 ± 0.522
1.159LeuTrp: 1.159 ± 0.3
1.594LeuTyr: 1.594 ± 0.377
0.0LeuXaa: 0.0 ± 0.0
Met
2.535MetAla: 2.535 ± 0.382
0.072MetCys: 0.072 ± 0.079
1.087MetAsp: 1.087 ± 0.263
1.811MetGlu: 1.811 ± 0.351
0.724MetPhe: 0.724 ± 0.239
1.594MetGly: 1.594 ± 0.318
0.29MetHis: 0.29 ± 0.139
0.797MetIle: 0.797 ± 0.254
1.449MetLys: 1.449 ± 0.516
2.39MetLeu: 2.39 ± 0.325
0.435MetMet: 0.435 ± 0.17
1.521MetAsn: 1.521 ± 0.281
1.666MetPro: 1.666 ± 0.352
1.304MetGln: 1.304 ± 0.343
1.811MetArg: 1.811 ± 0.304
1.956MetSer: 1.956 ± 0.415
1.666MetThr: 1.666 ± 0.371
1.956MetVal: 1.956 ± 0.36
0.29MetTrp: 0.29 ± 0.138
0.362MetTyr: 0.362 ± 0.191
0.0MetXaa: 0.0 ± 0.0
Asn
4.781AsnAla: 4.781 ± 0.637
0.435AsnCys: 0.435 ± 0.153
2.173AsnAsp: 2.173 ± 0.295
2.825AsnGlu: 2.825 ± 0.485
1.594AsnPhe: 1.594 ± 0.344
4.346AsnGly: 4.346 ± 0.798
0.869AsnHis: 0.869 ± 0.226
2.535AsnIle: 2.535 ± 0.422
1.739AsnLys: 1.739 ± 0.391
2.753AsnLeu: 2.753 ± 0.613
0.869AsnMet: 0.869 ± 0.246
1.594AsnAsn: 1.594 ± 0.292
2.318AsnPro: 2.318 ± 0.445
2.028AsnGln: 2.028 ± 0.35
2.97AsnArg: 2.97 ± 0.559
3.332AsnSer: 3.332 ± 0.824
2.318AsnThr: 2.318 ± 0.353
2.535AsnVal: 2.535 ± 0.461
0.29AsnTrp: 0.29 ± 0.123
1.376AsnTyr: 1.376 ± 0.274
0.0AsnXaa: 0.0 ± 0.0
Pro
4.491ProAla: 4.491 ± 0.76
0.507ProCys: 0.507 ± 0.234
2.68ProAsp: 2.68 ± 0.523
4.201ProGlu: 4.201 ± 0.817
1.159ProPhe: 1.159 ± 0.346
3.477ProGly: 3.477 ± 0.5
0.869ProHis: 0.869 ± 0.283
0.869ProIle: 0.869 ± 0.28
1.521ProLys: 1.521 ± 0.406
2.535ProLeu: 2.535 ± 0.591
0.942ProMet: 0.942 ± 0.305
1.231ProAsn: 1.231 ± 0.309
1.376ProPro: 1.376 ± 0.397
1.304ProGln: 1.304 ± 0.319
2.246ProArg: 2.246 ± 0.427
2.608ProSer: 2.608 ± 0.46
1.956ProThr: 1.956 ± 0.375
4.201ProVal: 4.201 ± 0.513
0.507ProTrp: 0.507 ± 0.249
1.304ProTyr: 1.304 ± 0.348
0.0ProXaa: 0.0 ± 0.0
Gln
4.564GlnAla: 4.564 ± 0.991
0.724GlnCys: 0.724 ± 0.203
1.159GlnAsp: 1.159 ± 0.263
2.753GlnGlu: 2.753 ± 0.456
1.594GlnPhe: 1.594 ± 0.35
2.68GlnGly: 2.68 ± 0.39
1.159GlnHis: 1.159 ± 0.239
2.753GlnIle: 2.753 ± 0.509
2.753GlnLys: 2.753 ± 0.541
4.057GlnLeu: 4.057 ± 0.733
1.304GlnMet: 1.304 ± 0.349
1.376GlnAsn: 1.376 ± 0.377
1.376GlnPro: 1.376 ± 0.357
2.753GlnGln: 2.753 ± 1.002
2.68GlnArg: 2.68 ± 0.684
2.898GlnSer: 2.898 ± 0.409
2.101GlnThr: 2.101 ± 0.482
3.405GlnVal: 3.405 ± 0.501
0.942GlnTrp: 0.942 ± 0.264
0.942GlnTyr: 0.942 ± 0.226
0.0GlnXaa: 0.0 ± 0.0
Arg
4.926ArgAla: 4.926 ± 0.594
1.231ArgCys: 1.231 ± 0.368
3.477ArgAsp: 3.477 ± 0.58
3.984ArgGlu: 3.984 ± 0.432
2.101ArgPhe: 2.101 ± 0.318
3.984ArgGly: 3.984 ± 0.542
1.739ArgHis: 1.739 ± 0.403
3.332ArgIle: 3.332 ± 0.485
3.26ArgLys: 3.26 ± 0.36
5.867ArgLeu: 5.867 ± 0.603
2.608ArgMet: 2.608 ± 0.462
3.042ArgAsn: 3.042 ± 0.438
2.318ArgPro: 2.318 ± 0.448
3.622ArgGln: 3.622 ± 0.949
5.143ArgArg: 5.143 ± 1.084
2.535ArgSer: 2.535 ± 0.465
3.115ArgThr: 3.115 ± 0.67
3.405ArgVal: 3.405 ± 0.752
1.231ArgTrp: 1.231 ± 0.317
3.042ArgTyr: 3.042 ± 0.396
0.0ArgXaa: 0.0 ± 0.0
Ser
8.548SerAla: 8.548 ± 1.439
0.58SerCys: 0.58 ± 0.266
4.708SerAsp: 4.708 ± 0.445
4.926SerGlu: 4.926 ± 0.884
2.101SerPhe: 2.101 ± 0.414
6.954SerGly: 6.954 ± 0.754
0.869SerHis: 0.869 ± 0.223
2.825SerIle: 2.825 ± 0.509
3.042SerLys: 3.042 ± 0.47
5.578SerLeu: 5.578 ± 0.642
1.231SerMet: 1.231 ± 0.257
2.246SerAsn: 2.246 ± 0.395
2.173SerPro: 2.173 ± 0.584
3.115SerGln: 3.115 ± 0.391
3.912SerArg: 3.912 ± 0.661
4.998SerSer: 4.998 ± 1.01
3.694SerThr: 3.694 ± 0.706
5.795SerVal: 5.795 ± 0.824
0.435SerTrp: 0.435 ± 0.202
1.304SerTyr: 1.304 ± 0.27
0.0SerXaa: 0.0 ± 0.0
Thr
5.723ThrAla: 5.723 ± 0.892
0.217ThrCys: 0.217 ± 0.132
3.405ThrAsp: 3.405 ± 0.514
3.912ThrGlu: 3.912 ± 0.682
2.535ThrPhe: 2.535 ± 0.362
4.708ThrGly: 4.708 ± 0.666
1.521ThrHis: 1.521 ± 0.31
2.97ThrIle: 2.97 ± 0.5
2.173ThrLys: 2.173 ± 0.458
4.491ThrLeu: 4.491 ± 0.677
0.724ThrMet: 0.724 ± 0.22
1.739ThrAsn: 1.739 ± 0.588
3.26ThrPro: 3.26 ± 0.817
2.246ThrGln: 2.246 ± 0.453
3.622ThrArg: 3.622 ± 0.61
3.839ThrSer: 3.839 ± 0.72
3.332ThrThr: 3.332 ± 0.653
4.636ThrVal: 4.636 ± 0.907
1.087ThrTrp: 1.087 ± 0.264
1.811ThrTyr: 1.811 ± 0.341
0.0ThrXaa: 0.0 ± 0.0
Val
5.795ValAla: 5.795 ± 0.639
0.869ValCys: 0.869 ± 0.237
3.332ValAsp: 3.332 ± 0.636
3.694ValGlu: 3.694 ± 0.589
2.68ValPhe: 2.68 ± 0.578
3.984ValGly: 3.984 ± 0.563
0.797ValHis: 0.797 ± 0.243
4.564ValIle: 4.564 ± 0.555
4.998ValLys: 4.998 ± 0.691
6.447ValLeu: 6.447 ± 0.651
1.521ValMet: 1.521 ± 0.339
3.115ValAsn: 3.115 ± 0.486
2.97ValPro: 2.97 ± 0.727
2.97ValGln: 2.97 ± 0.572
4.057ValArg: 4.057 ± 0.557
5.578ValSer: 5.578 ± 0.576
4.491ValThr: 4.491 ± 0.591
3.912ValVal: 3.912 ± 0.566
1.159ValTrp: 1.159 ± 0.262
1.666ValTyr: 1.666 ± 0.363
0.0ValXaa: 0.0 ± 0.0
Trp
1.231TrpAla: 1.231 ± 0.277
0.217TrpCys: 0.217 ± 0.121
0.797TrpAsp: 0.797 ± 0.304
0.724TrpGlu: 0.724 ± 0.198
0.869TrpPhe: 0.869 ± 0.273
1.376TrpGly: 1.376 ± 0.278
0.362TrpHis: 0.362 ± 0.212
1.014TrpIle: 1.014 ± 0.242
0.942TrpLys: 0.942 ± 0.227
2.318TrpLeu: 2.318 ± 0.54
0.58TrpMet: 0.58 ± 0.203
0.58TrpAsn: 0.58 ± 0.208
0.58TrpPro: 0.58 ± 0.367
0.724TrpGln: 0.724 ± 0.218
0.942TrpArg: 0.942 ± 0.34
1.014TrpSer: 1.014 ± 0.22
0.724TrpThr: 0.724 ± 0.21
0.942TrpVal: 0.942 ± 0.324
0.507TrpTrp: 0.507 ± 0.237
0.724TrpTyr: 0.724 ± 0.249
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.535TyrAla: 2.535 ± 0.483
0.217TyrCys: 0.217 ± 0.155
1.521TyrAsp: 1.521 ± 0.368
1.521TyrGlu: 1.521 ± 0.409
1.231TyrPhe: 1.231 ± 0.356
2.101TyrGly: 2.101 ± 0.401
0.507TyrHis: 0.507 ± 0.165
1.739TyrIle: 1.739 ± 0.402
1.304TyrLys: 1.304 ± 0.367
2.68TyrLeu: 2.68 ± 0.381
0.145TyrMet: 0.145 ± 0.109
1.231TyrAsn: 1.231 ± 0.293
0.797TyrPro: 0.797 ± 0.203
1.449TyrGln: 1.449 ± 0.283
1.956TyrArg: 1.956 ± 0.374
2.318TyrSer: 2.318 ± 0.464
2.028TyrThr: 2.028 ± 0.355
1.521TyrVal: 1.521 ± 0.302
0.435TyrTrp: 0.435 ± 0.173
0.724TyrTyr: 0.724 ± 0.296
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.072XaaArg: 0.072 ± 0.071
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (13806 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski