Oceanibaculum pacificum
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3560 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A154VS37|A0A154VS37_9PROT LysR family transcriptional regulator OS=Oceanibaculum pacificum OX=580166 GN=AUP43_03185 PE=3 SV=1
MM1 pKa = 7.57 PVWQAYY7 pKa = 5.27 TGKK10 pKa = 9.75 GVKK13 pKa = 9.98 VGIYY17 pKa = 10.14 DD18 pKa = 3.49 SGVQISHH25 pKa = 7.8 PDD27 pKa = 3.37 LLVNLDD33 pKa = 3.99 PALQPTTVSGTHH45 pKa = 6.96 DD46 pKa = 4.64 PDD48 pKa = 4.18 PVDD51 pKa = 3.99 GRR53 pKa = 11.84 FVTNDD58 pKa = 2.87 GHH60 pKa = 5.44 GTAVAGLILAANNGFGVVGVAYY82 pKa = 9.92 DD83 pKa = 3.52 ATFGAVNLILSDD95 pKa = 3.52 TYY97 pKa = 11.1 EE98 pKa = 4.37 SQNEE102 pKa = 4.16 TLDD105 pKa = 3.85 MIEE108 pKa = 4.19 LQALRR113 pKa = 11.84 YY114 pKa = 9.37 QMARR118 pKa = 11.84 FDD120 pKa = 4.82 VINHH124 pKa = 6.19 SYY126 pKa = 10.7 GPNEE130 pKa = 3.62 PFIANFTEE138 pKa = 4.2 YY139 pKa = 11.11 EE140 pKa = 4.09 NFAEE144 pKa = 4.66 LAAFDD149 pKa = 4.34 RR150 pKa = 11.84 ASLMGRR156 pKa = 11.84 NGLGTVHH163 pKa = 6.18 VVAGGNEE170 pKa = 3.74 RR171 pKa = 11.84 DD172 pKa = 3.64 QGNMTTYY179 pKa = 11.37 GDD181 pKa = 4.18 LGNLRR186 pKa = 11.84 YY187 pKa = 8.31 TINVAAGSAEE197 pKa = 3.66 GDD199 pKa = 3.2 IIYY202 pKa = 10.37 YY203 pKa = 9.87 SNPGASLVATAPVDD217 pKa = 4.31 RR218 pKa = 11.84 NPDD221 pKa = 3.35 DD222 pKa = 5.76 DD223 pKa = 4.19 GFKK226 pKa = 10.9 SVTTDD231 pKa = 3.46 LLGSLGYY238 pKa = 10.13 SGSNAEE244 pKa = 5.0 EE245 pKa = 4.22 ITSDD249 pKa = 3.76 AYY251 pKa = 11.46 SDD253 pKa = 3.44
Molecular weight: 26.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 4.012
IPC_protein 4.012
Toseland 3.795
ProMoST 4.139
Dawson 4.012
Bjellqvist 4.19
Wikipedia 3.948
Rodwell 3.846
Grimsley 3.706
Solomon 3.999
Lehninger 3.961
Nozaki 4.126
DTASelect 4.38
Thurlkill 3.846
EMBOSS 3.961
Sillero 4.139
Patrickios 1.214
IPC_peptide 3.999
IPC2_peptide 4.113
IPC2.peptide.svr19 4.024
Protein with the highest isoelectric point:
>tr|A0A154VYF9|A0A154VYF9_9PROT Uncharacterized protein OS=Oceanibaculum pacificum OX=580166 GN=AUP43_11020 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 8.7 IVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.49 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.32 VIANRR34 pKa = 11.84 RR35 pKa = 11.84 SRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.9 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3560
0
3560
1140145
29
5060
320.3
34.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.756 ± 0.071
0.823 ± 0.012
5.704 ± 0.04
5.722 ± 0.037
3.568 ± 0.03
8.798 ± 0.046
1.941 ± 0.019
5.202 ± 0.03
3.199 ± 0.04
10.779 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.578 ± 0.02
2.436 ± 0.028
5.317 ± 0.044
3.268 ± 0.025
7.091 ± 0.05
4.862 ± 0.025
5.098 ± 0.039
7.25 ± 0.04
1.268 ± 0.016
2.339 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here