Alkalispirochaeta americana

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Spirochaetaceae; Alkalispirochaeta

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2891 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1N6PQN8|A0A1N6PQN8_9SPIO Uncharacterized protein OS=Alkalispirochaeta americana OX=159291 GN=SAMN05920897_10386 PE=4 SV=1
MM1 pKa = 6.76KK2 pKa = 9.68TKK4 pKa = 10.54NALFSACPSLHH15 pKa = 6.41SSFFLGVAWFFLIFLALGGCYY36 pKa = 7.32WTPDD40 pKa = 3.3EE41 pKa = 4.51SRR43 pKa = 11.84GSISLRR49 pKa = 11.84IQAPSSGEE57 pKa = 3.76QVTASDD63 pKa = 4.11TVGEE67 pKa = 4.1PAYY70 pKa = 10.76LLLVYY75 pKa = 10.4VIEE78 pKa = 4.33EE79 pKa = 3.84AFIRR83 pKa = 11.84QDD85 pKa = 3.12PEE87 pKa = 4.01GNMEE91 pKa = 5.02HH92 pKa = 7.56IFGLWEE98 pKa = 3.88ASFDD102 pKa = 3.81EE103 pKa = 4.66EE104 pKa = 4.75DD105 pKa = 4.39FEE107 pKa = 5.6GADD110 pKa = 3.84SSPGSLGSISLGTSALASQFQGFAEE135 pKa = 4.04LLGYY139 pKa = 9.9EE140 pKa = 4.39SSAGGTILFSNLNPDD155 pKa = 2.63SSYY158 pKa = 11.23LVVAEE163 pKa = 4.0VHH165 pKa = 6.21SLEE168 pKa = 4.15EE169 pKa = 3.98LVGEE173 pKa = 4.24GQEE176 pKa = 3.84DD177 pKa = 3.2SRR179 pKa = 11.84YY180 pKa = 9.87SFRR183 pKa = 11.84TTTVRR188 pKa = 11.84SGEE191 pKa = 4.01NRR193 pKa = 11.84TVNLDD198 pKa = 3.24LDD200 pKa = 3.92KK201 pKa = 11.11TLNQYY206 pKa = 11.22LEE208 pKa = 4.31FLNARR213 pKa = 11.84YY214 pKa = 9.54GLSEE218 pKa = 4.9PDD220 pKa = 4.12DD221 pKa = 4.65PSDD224 pKa = 3.61GSIEE228 pKa = 3.8ITANLVNPEE237 pKa = 4.18DD238 pKa = 4.04EE239 pKa = 4.93EE240 pKa = 5.35IEE242 pKa = 4.17LTADD246 pKa = 3.31AEE248 pKa = 4.64VNLDD252 pKa = 4.27EE253 pKa = 5.67ISEE256 pKa = 4.35LTFTAPEE263 pKa = 5.09GYY265 pKa = 10.1DD266 pKa = 3.39SYY268 pKa = 11.78EE269 pKa = 3.66WTVNNMEE276 pKa = 4.34GWDD279 pKa = 3.78NSNTITISEE288 pKa = 4.36SEE290 pKa = 3.93ISDD293 pKa = 3.85VIFPGLNTVDD303 pKa = 4.97LEE305 pKa = 4.47VEE307 pKa = 4.12LGGEE311 pKa = 4.61VYY313 pKa = 10.64HH314 pKa = 6.34GTFTFTAIEE323 pKa = 4.46TILGEE328 pKa = 4.15LVIEE332 pKa = 5.19AILVNPEE339 pKa = 4.27DD340 pKa = 3.85EE341 pKa = 5.07QITLDD346 pKa = 4.15ADD348 pKa = 3.7AEE350 pKa = 4.57VNLDD354 pKa = 4.27EE355 pKa = 5.67ISEE358 pKa = 4.35LTFTAPEE365 pKa = 5.21GYY367 pKa = 10.18DD368 pKa = 3.41SYY370 pKa = 12.02NWTVNNMEE378 pKa = 4.57DD379 pKa = 3.16WGNSNTITISEE390 pKa = 4.17SDD392 pKa = 3.2ISDD395 pKa = 3.73VIFPGLNTVDD405 pKa = 4.97LEE407 pKa = 4.47VEE409 pKa = 4.12LGGEE413 pKa = 4.61VYY415 pKa = 10.64HH416 pKa = 6.34GTFTFTAIEE425 pKa = 4.46TILGEE430 pKa = 4.15LVIEE434 pKa = 5.19AILVNPEE441 pKa = 3.96DD442 pKa = 4.07EE443 pKa = 4.9EE444 pKa = 5.27IEE446 pKa = 4.16LTADD450 pKa = 3.3TEE452 pKa = 4.58VNIDD456 pKa = 4.43EE457 pKa = 4.32ITEE460 pKa = 4.19LTFTAPTGYY469 pKa = 10.44DD470 pKa = 3.17SYY472 pKa = 11.29EE473 pKa = 3.71WTVNDD478 pKa = 3.72MEE480 pKa = 4.9GWDD483 pKa = 3.83NSNTITIPEE492 pKa = 4.1SDD494 pKa = 3.27ISDD497 pKa = 3.99VIKK500 pKa = 10.84LGQNTVDD507 pKa = 4.13LVVEE511 pKa = 4.27KK512 pKa = 10.98AGALYY517 pKa = 9.69SHH519 pKa = 7.48RR520 pKa = 11.84FTFTAISDD528 pKa = 3.62

Molecular weight:
58.0 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1N6RXP6|A0A1N6RXP6_9SPIO Alpha amylase catalytic domain OS=Alkalispirochaeta americana OX=159291 GN=SAMN05920897_10755 PE=4 SV=1
MM1 pKa = 7.9PPQNIYY7 pKa = 10.07PCYY10 pKa = 9.66GGQGNILVGTAQTAGYY26 pKa = 8.74FVHH29 pKa = 6.51PHH31 pKa = 6.51PFGSQEE37 pKa = 3.72KK38 pKa = 10.05LKK40 pKa = 10.78IEE42 pKa = 4.86GYY44 pKa = 8.84PPKK47 pKa = 10.86AEE49 pKa = 4.33GPGDD53 pKa = 3.92ALRR56 pKa = 11.84DD57 pKa = 3.9VPDD60 pKa = 4.31LPGPAPGKK68 pKa = 9.68ARR70 pKa = 11.84GVAVCQAHH78 pKa = 6.07AQALTARR85 pKa = 11.84EE86 pKa = 4.23VGRR89 pKa = 11.84EE90 pKa = 4.31GYY92 pKa = 8.86PVRR95 pKa = 11.84HH96 pKa = 6.07GPDD99 pKa = 3.45LGLCPLPGRR108 pKa = 11.84PPAEE112 pKa = 3.73GHH114 pKa = 6.59LPGGVVFFHH123 pKa = 6.45QEE125 pKa = 3.0ARR127 pKa = 11.84LPGKK131 pKa = 10.47GEE133 pKa = 4.12SPGPMVQKK141 pKa = 10.83LPTIVDD147 pKa = 3.36NRR149 pKa = 11.84NAPLALAVQGFYY161 pKa = 11.33YY162 pKa = 9.89PGKK165 pKa = 9.83AQLRR169 pKa = 11.84QPLLIPPRR177 pKa = 11.84GVITQNRR184 pKa = 11.84PGNGGNTGMAKK195 pKa = 10.31PLFHH199 pKa = 7.1VSLVGNKK206 pKa = 8.77QSLPGRR212 pKa = 11.84KK213 pKa = 8.97RR214 pKa = 11.84IGEE217 pKa = 4.07PPILRR222 pKa = 11.84SPGCGIHH229 pKa = 6.53GLVGPGRR236 pKa = 11.84HH237 pKa = 5.39NCIDD241 pKa = 3.46SQLPCRR247 pKa = 11.84PGDD250 pKa = 3.08RR251 pKa = 11.84SMIEE255 pKa = 3.67GRR257 pKa = 11.84GLHH260 pKa = 6.02IAISQDD266 pKa = 2.88PPRR269 pKa = 11.84RR270 pKa = 11.84VFSLIGQDD278 pKa = 3.4HH279 pKa = 6.29QKK281 pKa = 10.24PLPAGPLQNPQLAATTPQNHH301 pKa = 6.33KK302 pKa = 9.71PGPGEE307 pKa = 3.91RR308 pKa = 11.84APGTARR314 pKa = 11.84NPPQGPQRR322 pKa = 11.84PPIEE326 pKa = 4.11QEE328 pKa = 3.78QPVYY332 pKa = 10.45LQEE335 pKa = 4.17PRR337 pKa = 11.84QGIAAATRR345 pKa = 11.84GSAPGRR351 pKa = 11.84GSAAAALVVATRR363 pKa = 11.84GSAAAFLVVATRR375 pKa = 11.84GSAAALVVATRR386 pKa = 11.84GSAAGLVVATRR397 pKa = 11.84GSAAALVVATRR408 pKa = 11.84GSAAAFLVVATRR420 pKa = 11.84GSMAASLVVATRR432 pKa = 11.84GSAAALVVATRR443 pKa = 11.84GSTPGRR449 pKa = 11.84GSAASLVVATRR460 pKa = 11.84GSAAAFLVVATRR472 pKa = 11.84GSTTGRR478 pKa = 11.84GSAAAFLVVATRR490 pKa = 11.84GSAAALVVATRR501 pKa = 11.84GSAAALVVATRR512 pKa = 11.84GSMAAFLVVATRR524 pKa = 11.84GSAAALVVATRR535 pKa = 11.84GSAAALVVATRR546 pKa = 11.84GSAAALVVATRR557 pKa = 11.84GSAAGRR563 pKa = 11.84GSAAASLVVATRR575 pKa = 11.84GSAAALVVATRR586 pKa = 11.84GSTPGRR592 pKa = 11.84GSAAAFLVVATRR604 pKa = 11.84GSAAGRR610 pKa = 11.84GSAPGRR616 pKa = 11.84GSAAALVVATRR627 pKa = 11.84GSTTGRR633 pKa = 11.84GSAAAFLVVATRR645 pKa = 11.84GSAAALVVATRR656 pKa = 11.84GSAAAFLATRR666 pKa = 11.84PLAVAHH672 pKa = 6.51RR673 pKa = 11.84RR674 pKa = 11.84RR675 pKa = 11.84NRR677 pKa = 11.84PEE679 pKa = 3.82LQEE682 pKa = 3.79HH683 pKa = 5.68RR684 pKa = 11.84RR685 pKa = 11.84KK686 pKa = 9.31IRR688 pKa = 11.84KK689 pKa = 8.48RR690 pKa = 11.84HH691 pKa = 5.4GPLQRR696 pKa = 11.84PHH698 pKa = 5.72QQLPPKK704 pKa = 9.41HH705 pKa = 6.55AKK707 pKa = 7.76TAHH710 pKa = 6.07GNAPGQPQKK719 pKa = 11.3KK720 pKa = 8.24MKK722 pKa = 9.5QKK724 pKa = 10.05RR725 pKa = 11.84VQPRR729 pKa = 11.84SQPPHH734 pKa = 6.62PPGLSARR741 pKa = 11.84NTVDD745 pKa = 2.88QRR747 pKa = 11.84TARR750 pKa = 11.84HH751 pKa = 6.04LLQTAAQEE759 pKa = 4.22RR760 pKa = 11.84NHH762 pKa = 6.75LGRR765 pKa = 11.84VGPIIVQRR773 pKa = 11.84NHH775 pKa = 5.44QLPPRR780 pKa = 11.84RR781 pKa = 11.84GHH783 pKa = 6.6PRR785 pKa = 11.84QSSPDD790 pKa = 3.55LPCPSGHH797 pKa = 5.98QNQPNPGPGHH807 pKa = 7.25PGQSLPVNRR816 pKa = 11.84ITPVVNDD823 pKa = 3.39NHH825 pKa = 6.79LAPASLGQNRR835 pKa = 11.84PHH837 pKa = 7.37PGHH840 pKa = 6.99KK841 pKa = 9.69PGQTNTPVQGRR852 pKa = 11.84NNQRR856 pKa = 11.84KK857 pKa = 8.97KK858 pKa = 10.23RR859 pKa = 11.84SSVHH863 pKa = 6.12GNHH866 pKa = 5.83STLMHH871 pKa = 7.5PDD873 pKa = 2.71QSMCYY878 pKa = 8.08TFSMDD883 pKa = 3.14HH884 pKa = 6.07ATDD887 pKa = 3.7LVLRR891 pKa = 11.84NPDD894 pKa = 3.54YY895 pKa = 11.32LLTPQGPITGQDD907 pKa = 2.83LWISRR912 pKa = 11.84GAITAILPQGSPPPPGTPTPRR933 pKa = 11.84SVDD936 pKa = 3.49CRR938 pKa = 11.84GKK940 pKa = 10.5AAIPGFKK947 pKa = 9.79NAHH950 pKa = 4.42THH952 pKa = 6.26AAMTLLRR959 pKa = 11.84GYY961 pKa = 10.48GDD963 pKa = 5.38DD964 pKa = 4.26MPLQAWLQEE973 pKa = 4.42RR974 pKa = 11.84IWPAEE979 pKa = 3.49AHH981 pKa = 5.45MTPEE985 pKa = 4.92DD986 pKa = 4.18IYY988 pKa = 10.77WGTRR992 pKa = 11.84LAALEE997 pKa = 4.27MIKK1000 pKa = 10.59SGTTFANDD1008 pKa = 3.56MYY1010 pKa = 10.59FHH1012 pKa = 7.56PAQAAQAFTDD1022 pKa = 3.36AGIRR1026 pKa = 11.84AALGLALFDD1035 pKa = 4.59FDD1037 pKa = 5.04DD1038 pKa = 3.74PHH1040 pKa = 8.4RR1041 pKa = 11.84RR1042 pKa = 11.84AQVQDD1047 pKa = 3.42RR1048 pKa = 11.84LQDD1051 pKa = 3.53NLEE1054 pKa = 4.47TIWPNRR1060 pKa = 11.84RR1061 pKa = 11.84KK1062 pKa = 10.22SPPEE1066 pKa = 3.51EE1067 pKa = 4.33PEE1069 pKa = 3.79QVFPVIAPHH1078 pKa = 7.35SIYY1081 pKa = 10.08TCSGEE1086 pKa = 3.97LLQWCARR1093 pKa = 11.84QASEE1097 pKa = 4.16NHH1099 pKa = 6.09LLYY1102 pKa = 10.1HH1103 pKa = 6.09IHH1105 pKa = 5.84MNEE1108 pKa = 3.45TRR1110 pKa = 11.84RR1111 pKa = 11.84EE1112 pKa = 3.88VDD1114 pKa = 2.54EE1115 pKa = 4.53CLAARR1120 pKa = 11.84GVPPMEE1126 pKa = 4.56HH1127 pKa = 6.04LQNLGVLEE1135 pKa = 4.28TMGDD1139 pKa = 3.46RR1140 pKa = 11.84VVAAHH1145 pKa = 6.17MVWLDD1150 pKa = 3.5PPEE1153 pKa = 5.71LDD1155 pKa = 3.67IAARR1159 pKa = 11.84WGITAVHH1166 pKa = 6.19NPASNMKK1173 pKa = 9.58LASGCFPWQDD1183 pKa = 3.34YY1184 pKa = 10.76ARR1186 pKa = 11.84RR1187 pKa = 11.84KK1188 pKa = 9.5APLMLATDD1196 pKa = 4.65GTASNNNLDD1205 pKa = 4.2MFDD1208 pKa = 3.8EE1209 pKa = 4.56MKK1211 pKa = 10.73LAALLQKK1218 pKa = 10.43HH1219 pKa = 6.18HH1220 pKa = 6.53FQDD1223 pKa = 3.87STRR1226 pKa = 11.84LPAEE1230 pKa = 4.6EE1231 pKa = 4.4ILAIATGARR1240 pKa = 11.84STVFEE1245 pKa = 4.04PWGVGGALTEE1255 pKa = 4.29GAPADD1260 pKa = 3.54IALIDD1265 pKa = 4.3LDD1267 pKa = 4.39HH1268 pKa = 6.68PQMVPVHH1275 pKa = 6.04NLQSNLVYY1283 pKa = 10.35SANGSVVDD1291 pKa = 4.65SVICNGRR1298 pKa = 11.84VLMEE1302 pKa = 3.96NRR1304 pKa = 11.84QVPGEE1309 pKa = 4.14EE1310 pKa = 3.73EE1311 pKa = 4.11VLRR1314 pKa = 11.84EE1315 pKa = 3.89ARR1317 pKa = 11.84RR1318 pKa = 11.84CTRR1321 pKa = 11.84EE1322 pKa = 3.75LVKK1325 pKa = 10.57RR1326 pKa = 11.84AAPP1329 pKa = 3.58

Molecular weight:
139.86 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2891

0

2891

1021938

26

2692

353.5

39.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.107 ± 0.047

0.84 ± 0.015

5.402 ± 0.038

7.207 ± 0.047

4.044 ± 0.033

8.085 ± 0.046

2.071 ± 0.021

5.593 ± 0.035

2.981 ± 0.038

10.911 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.142 ± 0.024

2.716 ± 0.028

5.058 ± 0.042

3.756 ± 0.028

7.672 ± 0.047

6.287 ± 0.038

5.182 ± 0.048

7.146 ± 0.045

1.155 ± 0.017

2.644 ± 0.026

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski