Alkalispirochaeta americana
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2891 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N6PQN8|A0A1N6PQN8_9SPIO Uncharacterized protein OS=Alkalispirochaeta americana OX=159291 GN=SAMN05920897_10386 PE=4 SV=1
MM1 pKa = 6.76 KK2 pKa = 9.68 TKK4 pKa = 10.54 NALFSACPSLHH15 pKa = 6.41 SSFFLGVAWFFLIFLALGGCYY36 pKa = 7.32 WTPDD40 pKa = 3.3 EE41 pKa = 4.51 SRR43 pKa = 11.84 GSISLRR49 pKa = 11.84 IQAPSSGEE57 pKa = 3.76 QVTASDD63 pKa = 4.11 TVGEE67 pKa = 4.1 PAYY70 pKa = 10.76 LLLVYY75 pKa = 10.4 VIEE78 pKa = 4.33 EE79 pKa = 3.84 AFIRR83 pKa = 11.84 QDD85 pKa = 3.12 PEE87 pKa = 4.01 GNMEE91 pKa = 5.02 HH92 pKa = 7.56 IFGLWEE98 pKa = 3.88 ASFDD102 pKa = 3.81 EE103 pKa = 4.66 EE104 pKa = 4.75 DD105 pKa = 4.39 FEE107 pKa = 5.6 GADD110 pKa = 3.84 SSPGSLGSISLGTSALASQFQGFAEE135 pKa = 4.04 LLGYY139 pKa = 9.9 EE140 pKa = 4.39 SSAGGTILFSNLNPDD155 pKa = 2.63 SSYY158 pKa = 11.23 LVVAEE163 pKa = 4.0 VHH165 pKa = 6.21 SLEE168 pKa = 4.15 EE169 pKa = 3.98 LVGEE173 pKa = 4.24 GQEE176 pKa = 3.84 DD177 pKa = 3.2 SRR179 pKa = 11.84 YY180 pKa = 9.87 SFRR183 pKa = 11.84 TTTVRR188 pKa = 11.84 SGEE191 pKa = 4.01 NRR193 pKa = 11.84 TVNLDD198 pKa = 3.24 LDD200 pKa = 3.92 KK201 pKa = 11.11 TLNQYY206 pKa = 11.22 LEE208 pKa = 4.31 FLNARR213 pKa = 11.84 YY214 pKa = 9.54 GLSEE218 pKa = 4.9 PDD220 pKa = 4.12 DD221 pKa = 4.65 PSDD224 pKa = 3.61 GSIEE228 pKa = 3.8 ITANLVNPEE237 pKa = 4.18 DD238 pKa = 4.04 EE239 pKa = 4.93 EE240 pKa = 5.35 IEE242 pKa = 4.17 LTADD246 pKa = 3.31 AEE248 pKa = 4.64 VNLDD252 pKa = 4.27 EE253 pKa = 5.67 ISEE256 pKa = 4.35 LTFTAPEE263 pKa = 5.09 GYY265 pKa = 10.1 DD266 pKa = 3.39 SYY268 pKa = 11.78 EE269 pKa = 3.66 WTVNNMEE276 pKa = 4.34 GWDD279 pKa = 3.78 NSNTITISEE288 pKa = 4.36 SEE290 pKa = 3.93 ISDD293 pKa = 3.85 VIFPGLNTVDD303 pKa = 4.97 LEE305 pKa = 4.47 VEE307 pKa = 4.12 LGGEE311 pKa = 4.61 VYY313 pKa = 10.64 HH314 pKa = 6.34 GTFTFTAIEE323 pKa = 4.46 TILGEE328 pKa = 4.15 LVIEE332 pKa = 5.19 AILVNPEE339 pKa = 4.27 DD340 pKa = 3.85 EE341 pKa = 5.07 QITLDD346 pKa = 4.15 ADD348 pKa = 3.7 AEE350 pKa = 4.57 VNLDD354 pKa = 4.27 EE355 pKa = 5.67 ISEE358 pKa = 4.35 LTFTAPEE365 pKa = 5.21 GYY367 pKa = 10.18 DD368 pKa = 3.41 SYY370 pKa = 12.02 NWTVNNMEE378 pKa = 4.57 DD379 pKa = 3.16 WGNSNTITISEE390 pKa = 4.17 SDD392 pKa = 3.2 ISDD395 pKa = 3.73 VIFPGLNTVDD405 pKa = 4.97 LEE407 pKa = 4.47 VEE409 pKa = 4.12 LGGEE413 pKa = 4.61 VYY415 pKa = 10.64 HH416 pKa = 6.34 GTFTFTAIEE425 pKa = 4.46 TILGEE430 pKa = 4.15 LVIEE434 pKa = 5.19 AILVNPEE441 pKa = 3.96 DD442 pKa = 4.07 EE443 pKa = 4.9 EE444 pKa = 5.27 IEE446 pKa = 4.16 LTADD450 pKa = 3.3 TEE452 pKa = 4.58 VNIDD456 pKa = 4.43 EE457 pKa = 4.32 ITEE460 pKa = 4.19 LTFTAPTGYY469 pKa = 10.44 DD470 pKa = 3.17 SYY472 pKa = 11.29 EE473 pKa = 3.71 WTVNDD478 pKa = 3.72 MEE480 pKa = 4.9 GWDD483 pKa = 3.83 NSNTITIPEE492 pKa = 4.1 SDD494 pKa = 3.27 ISDD497 pKa = 3.99 VIKK500 pKa = 10.84 LGQNTVDD507 pKa = 4.13 LVVEE511 pKa = 4.27 KK512 pKa = 10.98 AGALYY517 pKa = 9.69 SHH519 pKa = 7.48 RR520 pKa = 11.84 FTFTAISDD528 pKa = 3.62
Molecular weight: 58.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.816
IPC2_protein 3.541
IPC_protein 3.541
Toseland 3.338
ProMoST 3.656
Dawson 3.503
Bjellqvist 3.668
Wikipedia 3.401
Rodwell 3.363
Grimsley 3.249
Solomon 3.503
Lehninger 3.452
Nozaki 3.617
DTASelect 3.783
Thurlkill 3.376
EMBOSS 3.414
Sillero 3.656
Patrickios 0.718
IPC_peptide 3.503
IPC2_peptide 3.643
IPC2.peptide.svr19 3.65
Protein with the highest isoelectric point:
>tr|A0A1N6RXP6|A0A1N6RXP6_9SPIO Alpha amylase catalytic domain OS=Alkalispirochaeta americana OX=159291 GN=SAMN05920897_10755 PE=4 SV=1
MM1 pKa = 7.9 PPQNIYY7 pKa = 10.07 PCYY10 pKa = 9.66 GGQGNILVGTAQTAGYY26 pKa = 8.74 FVHH29 pKa = 6.51 PHH31 pKa = 6.51 PFGSQEE37 pKa = 3.72 KK38 pKa = 10.05 LKK40 pKa = 10.78 IEE42 pKa = 4.86 GYY44 pKa = 8.84 PPKK47 pKa = 10.86 AEE49 pKa = 4.33 GPGDD53 pKa = 3.92 ALRR56 pKa = 11.84 DD57 pKa = 3.9 VPDD60 pKa = 4.31 LPGPAPGKK68 pKa = 9.68 ARR70 pKa = 11.84 GVAVCQAHH78 pKa = 6.07 AQALTARR85 pKa = 11.84 EE86 pKa = 4.23 VGRR89 pKa = 11.84 EE90 pKa = 4.31 GYY92 pKa = 8.86 PVRR95 pKa = 11.84 HH96 pKa = 6.07 GPDD99 pKa = 3.45 LGLCPLPGRR108 pKa = 11.84 PPAEE112 pKa = 3.73 GHH114 pKa = 6.59 LPGGVVFFHH123 pKa = 6.45 QEE125 pKa = 3.0 ARR127 pKa = 11.84 LPGKK131 pKa = 10.47 GEE133 pKa = 4.12 SPGPMVQKK141 pKa = 10.83 LPTIVDD147 pKa = 3.36 NRR149 pKa = 11.84 NAPLALAVQGFYY161 pKa = 11.33 YY162 pKa = 9.89 PGKK165 pKa = 9.83 AQLRR169 pKa = 11.84 QPLLIPPRR177 pKa = 11.84 GVITQNRR184 pKa = 11.84 PGNGGNTGMAKK195 pKa = 10.31 PLFHH199 pKa = 7.1 VSLVGNKK206 pKa = 8.77 QSLPGRR212 pKa = 11.84 KK213 pKa = 8.97 RR214 pKa = 11.84 IGEE217 pKa = 4.07 PPILRR222 pKa = 11.84 SPGCGIHH229 pKa = 6.53 GLVGPGRR236 pKa = 11.84 HH237 pKa = 5.39 NCIDD241 pKa = 3.46 SQLPCRR247 pKa = 11.84 PGDD250 pKa = 3.08 RR251 pKa = 11.84 SMIEE255 pKa = 3.67 GRR257 pKa = 11.84 GLHH260 pKa = 6.02 IAISQDD266 pKa = 2.88 PPRR269 pKa = 11.84 RR270 pKa = 11.84 VFSLIGQDD278 pKa = 3.4 HH279 pKa = 6.29 QKK281 pKa = 10.24 PLPAGPLQNPQLAATTPQNHH301 pKa = 6.33 KK302 pKa = 9.71 PGPGEE307 pKa = 3.91 RR308 pKa = 11.84 APGTARR314 pKa = 11.84 NPPQGPQRR322 pKa = 11.84 PPIEE326 pKa = 4.11 QEE328 pKa = 3.78 QPVYY332 pKa = 10.45 LQEE335 pKa = 4.17 PRR337 pKa = 11.84 QGIAAATRR345 pKa = 11.84 GSAPGRR351 pKa = 11.84 GSAAAALVVATRR363 pKa = 11.84 GSAAAFLVVATRR375 pKa = 11.84 GSAAALVVATRR386 pKa = 11.84 GSAAGLVVATRR397 pKa = 11.84 GSAAALVVATRR408 pKa = 11.84 GSAAAFLVVATRR420 pKa = 11.84 GSMAASLVVATRR432 pKa = 11.84 GSAAALVVATRR443 pKa = 11.84 GSTPGRR449 pKa = 11.84 GSAASLVVATRR460 pKa = 11.84 GSAAAFLVVATRR472 pKa = 11.84 GSTTGRR478 pKa = 11.84 GSAAAFLVVATRR490 pKa = 11.84 GSAAALVVATRR501 pKa = 11.84 GSAAALVVATRR512 pKa = 11.84 GSMAAFLVVATRR524 pKa = 11.84 GSAAALVVATRR535 pKa = 11.84 GSAAALVVATRR546 pKa = 11.84 GSAAALVVATRR557 pKa = 11.84 GSAAGRR563 pKa = 11.84 GSAAASLVVATRR575 pKa = 11.84 GSAAALVVATRR586 pKa = 11.84 GSTPGRR592 pKa = 11.84 GSAAAFLVVATRR604 pKa = 11.84 GSAAGRR610 pKa = 11.84 GSAPGRR616 pKa = 11.84 GSAAALVVATRR627 pKa = 11.84 GSTTGRR633 pKa = 11.84 GSAAAFLVVATRR645 pKa = 11.84 GSAAALVVATRR656 pKa = 11.84 GSAAAFLATRR666 pKa = 11.84 PLAVAHH672 pKa = 6.51 RR673 pKa = 11.84 RR674 pKa = 11.84 RR675 pKa = 11.84 NRR677 pKa = 11.84 PEE679 pKa = 3.82 LQEE682 pKa = 3.79 HH683 pKa = 5.68 RR684 pKa = 11.84 RR685 pKa = 11.84 KK686 pKa = 9.31 IRR688 pKa = 11.84 KK689 pKa = 8.48 RR690 pKa = 11.84 HH691 pKa = 5.4 GPLQRR696 pKa = 11.84 PHH698 pKa = 5.72 QQLPPKK704 pKa = 9.41 HH705 pKa = 6.55 AKK707 pKa = 7.76 TAHH710 pKa = 6.07 GNAPGQPQKK719 pKa = 11.3 KK720 pKa = 8.24 MKK722 pKa = 9.5 QKK724 pKa = 10.05 RR725 pKa = 11.84 VQPRR729 pKa = 11.84 SQPPHH734 pKa = 6.62 PPGLSARR741 pKa = 11.84 NTVDD745 pKa = 2.88 QRR747 pKa = 11.84 TARR750 pKa = 11.84 HH751 pKa = 6.04 LLQTAAQEE759 pKa = 4.22 RR760 pKa = 11.84 NHH762 pKa = 6.75 LGRR765 pKa = 11.84 VGPIIVQRR773 pKa = 11.84 NHH775 pKa = 5.44 QLPPRR780 pKa = 11.84 RR781 pKa = 11.84 GHH783 pKa = 6.6 PRR785 pKa = 11.84 QSSPDD790 pKa = 3.55 LPCPSGHH797 pKa = 5.98 QNQPNPGPGHH807 pKa = 7.25 PGQSLPVNRR816 pKa = 11.84 ITPVVNDD823 pKa = 3.39 NHH825 pKa = 6.79 LAPASLGQNRR835 pKa = 11.84 PHH837 pKa = 7.37 PGHH840 pKa = 6.99 KK841 pKa = 9.69 PGQTNTPVQGRR852 pKa = 11.84 NNQRR856 pKa = 11.84 KK857 pKa = 8.97 KK858 pKa = 10.23 RR859 pKa = 11.84 SSVHH863 pKa = 6.12 GNHH866 pKa = 5.83 STLMHH871 pKa = 7.5 PDD873 pKa = 2.71 QSMCYY878 pKa = 8.08 TFSMDD883 pKa = 3.14 HH884 pKa = 6.07 ATDD887 pKa = 3.7 LVLRR891 pKa = 11.84 NPDD894 pKa = 3.54 YY895 pKa = 11.32 LLTPQGPITGQDD907 pKa = 2.83 LWISRR912 pKa = 11.84 GAITAILPQGSPPPPGTPTPRR933 pKa = 11.84 SVDD936 pKa = 3.49 CRR938 pKa = 11.84 GKK940 pKa = 10.5 AAIPGFKK947 pKa = 9.79 NAHH950 pKa = 4.42 THH952 pKa = 6.26 AAMTLLRR959 pKa = 11.84 GYY961 pKa = 10.48 GDD963 pKa = 5.38 DD964 pKa = 4.26 MPLQAWLQEE973 pKa = 4.42 RR974 pKa = 11.84 IWPAEE979 pKa = 3.49 AHH981 pKa = 5.45 MTPEE985 pKa = 4.92 DD986 pKa = 4.18 IYY988 pKa = 10.77 WGTRR992 pKa = 11.84 LAALEE997 pKa = 4.27 MIKK1000 pKa = 10.59 SGTTFANDD1008 pKa = 3.56 MYY1010 pKa = 10.59 FHH1012 pKa = 7.56 PAQAAQAFTDD1022 pKa = 3.36 AGIRR1026 pKa = 11.84 AALGLALFDD1035 pKa = 4.59 FDD1037 pKa = 5.04 DD1038 pKa = 3.74 PHH1040 pKa = 8.4 RR1041 pKa = 11.84 RR1042 pKa = 11.84 AQVQDD1047 pKa = 3.42 RR1048 pKa = 11.84 LQDD1051 pKa = 3.53 NLEE1054 pKa = 4.47 TIWPNRR1060 pKa = 11.84 RR1061 pKa = 11.84 KK1062 pKa = 10.22 SPPEE1066 pKa = 3.51 EE1067 pKa = 4.33 PEE1069 pKa = 3.79 QVFPVIAPHH1078 pKa = 7.35 SIYY1081 pKa = 10.08 TCSGEE1086 pKa = 3.97 LLQWCARR1093 pKa = 11.84 QASEE1097 pKa = 4.16 NHH1099 pKa = 6.09 LLYY1102 pKa = 10.1 HH1103 pKa = 6.09 IHH1105 pKa = 5.84 MNEE1108 pKa = 3.45 TRR1110 pKa = 11.84 RR1111 pKa = 11.84 EE1112 pKa = 3.88 VDD1114 pKa = 2.54 EE1115 pKa = 4.53 CLAARR1120 pKa = 11.84 GVPPMEE1126 pKa = 4.56 HH1127 pKa = 6.04 LQNLGVLEE1135 pKa = 4.28 TMGDD1139 pKa = 3.46 RR1140 pKa = 11.84 VVAAHH1145 pKa = 6.17 MVWLDD1150 pKa = 3.5 PPEE1153 pKa = 5.71 LDD1155 pKa = 3.67 IAARR1159 pKa = 11.84 WGITAVHH1166 pKa = 6.19 NPASNMKK1173 pKa = 9.58 LASGCFPWQDD1183 pKa = 3.34 YY1184 pKa = 10.76 ARR1186 pKa = 11.84 RR1187 pKa = 11.84 KK1188 pKa = 9.5 APLMLATDD1196 pKa = 4.65 GTASNNNLDD1205 pKa = 4.2 MFDD1208 pKa = 3.8 EE1209 pKa = 4.56 MKK1211 pKa = 10.73 LAALLQKK1218 pKa = 10.43 HH1219 pKa = 6.18 HH1220 pKa = 6.53 FQDD1223 pKa = 3.87 STRR1226 pKa = 11.84 LPAEE1230 pKa = 4.6 EE1231 pKa = 4.4 ILAIATGARR1240 pKa = 11.84 STVFEE1245 pKa = 4.04 PWGVGGALTEE1255 pKa = 4.29 GAPADD1260 pKa = 3.54 IALIDD1265 pKa = 4.3 LDD1267 pKa = 4.39 HH1268 pKa = 6.68 PQMVPVHH1275 pKa = 6.04 NLQSNLVYY1283 pKa = 10.35 SANGSVVDD1291 pKa = 4.65 SVICNGRR1298 pKa = 11.84 VLMEE1302 pKa = 3.96 NRR1304 pKa = 11.84 QVPGEE1309 pKa = 4.14 EE1310 pKa = 3.73 EE1311 pKa = 4.11 VLRR1314 pKa = 11.84 EE1315 pKa = 3.89 ARR1317 pKa = 11.84 RR1318 pKa = 11.84 CTRR1321 pKa = 11.84 EE1322 pKa = 3.75 LVKK1325 pKa = 10.57 RR1326 pKa = 11.84 AAPP1329 pKa = 3.58
Molecular weight: 139.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.589
IPC_protein 10.584
Toseland 10.745
ProMoST 10.57
Dawson 10.818
Bjellqvist 10.599
Wikipedia 11.082
Rodwell 10.891
Grimsley 10.877
Solomon 11.008
Lehninger 10.965
Nozaki 10.745
DTASelect 10.584
Thurlkill 10.745
EMBOSS 11.169
Sillero 10.774
Patrickios 10.555
IPC_peptide 11.008
IPC2_peptide 9.823
IPC2.peptide.svr19 8.686
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2891
0
2891
1021938
26
2692
353.5
39.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.107 ± 0.047
0.84 ± 0.015
5.402 ± 0.038
7.207 ± 0.047
4.044 ± 0.033
8.085 ± 0.046
2.071 ± 0.021
5.593 ± 0.035
2.981 ± 0.038
10.911 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.142 ± 0.024
2.716 ± 0.028
5.058 ± 0.042
3.756 ± 0.028
7.672 ± 0.047
6.287 ± 0.038
5.182 ± 0.048
7.146 ± 0.045
1.155 ± 0.017
2.644 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here