Chitinophagaceae bacterium IBVUCB2
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3527 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y2RA62|A0A1Y2RA62_9BACT Naringenin-chalcone synthase OS=Chitinophagaceae bacterium IBVUCB2 OX=1985174 GN=CAP36_14800 PE=3 SV=1
MM1 pKa = 7.2 SQIKK5 pKa = 9.86 LISYY9 pKa = 9.48 ISQSATFKK17 pKa = 10.64 ILYY20 pKa = 9.19 PKK22 pKa = 10.65 DD23 pKa = 3.82 FILNEE28 pKa = 4.38 DD29 pKa = 3.29 EE30 pKa = 5.44 DD31 pKa = 5.54 GIVTITSPITYY42 pKa = 10.44 SNLTLSGYY50 pKa = 9.95 HH51 pKa = 6.18 GNIEE55 pKa = 3.84 IDD57 pKa = 3.54 EE58 pKa = 4.39 KK59 pKa = 11.43 VLTEE63 pKa = 4.08 FFEE66 pKa = 5.02 DD67 pKa = 3.14 ATEE70 pKa = 4.22 AYY72 pKa = 9.66 IPISPTTKK80 pKa = 10.6 DD81 pKa = 2.7 ITTDD85 pKa = 2.96 RR86 pKa = 11.84 LYY88 pKa = 10.43 IEE90 pKa = 4.4 QAFKK94 pKa = 10.71 QDD96 pKa = 3.64 NINWIWWAVAKK107 pKa = 9.98 EE108 pKa = 3.98 NQIIMISVNSEE119 pKa = 4.15 DD120 pKa = 3.88 EE121 pKa = 4.4 LSEE124 pKa = 3.93 EE125 pKa = 4.64 DD126 pKa = 3.77 YY127 pKa = 11.62 NLYY130 pKa = 10.51 RR131 pKa = 11.84 YY132 pKa = 9.36 MIDD135 pKa = 3.35 QIEE138 pKa = 4.4 IYY140 pKa = 9.89 PAEE143 pKa = 4.38 FEE145 pKa = 4.48 DD146 pKa = 4.28
Molecular weight: 17.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.783
IPC2_protein 3.961
IPC_protein 3.91
Toseland 3.719
ProMoST 3.986
Dawson 3.872
Bjellqvist 4.062
Wikipedia 3.77
Rodwell 3.745
Grimsley 3.63
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.151
Thurlkill 3.757
EMBOSS 3.783
Sillero 4.024
Patrickios 1.888
IPC_peptide 3.859
IPC2_peptide 3.999
IPC2.peptide.svr19 3.924
Protein with the highest isoelectric point:
>tr|A0A1Y2RDF3|A0A1Y2RDF3_9BACT Uncharacterized protein OS=Chitinophagaceae bacterium IBVUCB2 OX=1985174 GN=CAP36_01450 PE=4 SV=1
MM1 pKa = 7.27 KK2 pKa = 10.39 RR3 pKa = 11.84 IFTLFTALLLTVALFAADD21 pKa = 3.9 RR22 pKa = 11.84 RR23 pKa = 11.84 PVVTVNSSKK32 pKa = 10.71 NYY34 pKa = 10.04 KK35 pKa = 9.71 IVIDD39 pKa = 3.8 GRR41 pKa = 11.84 SYY43 pKa = 11.18 YY44 pKa = 11.25 SNDD47 pKa = 3.1 RR48 pKa = 11.84 NIRR51 pKa = 11.84 LANLHH56 pKa = 6.19 NGRR59 pKa = 11.84 HH60 pKa = 4.95 TIKK63 pKa = 10.17 VFEE66 pKa = 4.57 MKK68 pKa = 10.56 RR69 pKa = 11.84 MGYY72 pKa = 7.32 NQRR75 pKa = 11.84 EE76 pKa = 4.24 RR77 pKa = 11.84 LVAATSFHH85 pKa = 6.06 VNRR88 pKa = 11.84 KK89 pKa = 8.21 NVHH92 pKa = 5.43 INIDD96 pKa = 3.28 RR97 pKa = 11.84 FGNINIRR104 pKa = 11.84 EE105 pKa = 3.96 VRR107 pKa = 11.84 NNGRR111 pKa = 11.84 FDD113 pKa = 4.32 NDD115 pKa = 3.63 RR116 pKa = 11.84 DD117 pKa = 3.46 WDD119 pKa = 3.89 DD120 pKa = 3.83 RR121 pKa = 11.84 DD122 pKa = 3.63 YY123 pKa = 11.74 RR124 pKa = 11.84 DD125 pKa = 3.55 DD126 pKa = 3.44 NRR128 pKa = 11.84 RR129 pKa = 11.84 FF130 pKa = 3.3
Molecular weight: 15.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.78
IPC_protein 10.818
Toseland 10.804
ProMoST 10.687
Dawson 10.891
Bjellqvist 10.672
Wikipedia 11.169
Rodwell 10.935
Grimsley 10.95
Solomon 11.082
Lehninger 11.038
Nozaki 10.774
DTASelect 10.672
Thurlkill 10.804
EMBOSS 11.228
Sillero 10.833
Patrickios 10.657
IPC_peptide 11.082
IPC2_peptide 9.692
IPC2.peptide.svr19 8.563
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3527
0
3527
1204414
38
2523
341.5
38.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.315 ± 0.038
0.872 ± 0.016
5.184 ± 0.03
5.812 ± 0.051
4.998 ± 0.033
6.859 ± 0.041
1.768 ± 0.02
7.446 ± 0.035
7.639 ± 0.05
9.033 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.413 ± 0.022
5.71 ± 0.041
3.79 ± 0.025
3.614 ± 0.024
3.726 ± 0.026
6.301 ± 0.038
6.027 ± 0.053
6.308 ± 0.03
1.251 ± 0.02
3.933 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here