Mouse mammary tumor virus (strain BR6) (MMTV)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Betaretrovirus; Mouse mammary tumor virus

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P10259|ENV_MMTVB Envelope glycoprotein gp70 OS=Mouse mammary tumor virus (strain BR6) OX=11758 GN=env PE=3 SV=1
MM1 pKa = 7.24GVSGSKK7 pKa = 9.48GQKK10 pKa = 10.01LFVSVLQRR18 pKa = 11.84LLSEE22 pKa = 4.7RR23 pKa = 11.84GLHH26 pKa = 5.31VKK28 pKa = 10.05EE29 pKa = 4.39SSAIEE34 pKa = 4.43FYY36 pKa = 11.02QFLIKK41 pKa = 10.55VSPWFPEE48 pKa = 3.79EE49 pKa = 4.5GGLNLQDD56 pKa = 3.26WKK58 pKa = 11.05RR59 pKa = 11.84VGRR62 pKa = 11.84EE63 pKa = 3.69MKK65 pKa = 10.22RR66 pKa = 11.84YY67 pKa = 8.78AAEE70 pKa = 4.68HH71 pKa = 5.81GTDD74 pKa = 4.27SIPKK78 pKa = 7.84QAYY81 pKa = 7.84PIWLQLRR88 pKa = 11.84EE89 pKa = 4.17ILTEE93 pKa = 3.88QSDD96 pKa = 4.44LVLLSAEE103 pKa = 4.15AKK105 pKa = 10.18SVTEE109 pKa = 4.08EE110 pKa = 3.97EE111 pKa = 4.47LEE113 pKa = 4.17EE114 pKa = 4.46GLTGLLSTSSQEE126 pKa = 3.65KK127 pKa = 9.03TYY129 pKa = 8.93GTRR132 pKa = 11.84GTAYY136 pKa = 10.45AEE138 pKa = 4.13IDD140 pKa = 3.78TEE142 pKa = 4.13VDD144 pKa = 3.01KK145 pKa = 11.4LSEE148 pKa = 4.53HH149 pKa = 7.04IYY151 pKa = 10.55DD152 pKa = 3.66EE153 pKa = 4.71PYY155 pKa = 10.62EE156 pKa = 4.21EE157 pKa = 5.61KK158 pKa = 10.79EE159 pKa = 4.01KK160 pKa = 10.96ADD162 pKa = 3.5KK163 pKa = 11.34NEE165 pKa = 4.22EE166 pKa = 3.82KK167 pKa = 11.02DD168 pKa = 3.34HH169 pKa = 5.29VRR171 pKa = 11.84KK172 pKa = 9.54IKK174 pKa = 10.65KK175 pKa = 8.89VVQRR179 pKa = 11.84KK180 pKa = 8.51EE181 pKa = 3.6NSEE184 pKa = 4.08GKK186 pKa = 10.25RR187 pKa = 11.84KK188 pKa = 10.13EE189 pKa = 4.06KK190 pKa = 10.42DD191 pKa = 3.16SKK193 pKa = 11.67AFLATDD199 pKa = 3.36WNDD202 pKa = 3.66DD203 pKa = 3.89DD204 pKa = 6.41LSPEE208 pKa = 4.02DD209 pKa = 3.83WDD211 pKa = 4.93DD212 pKa = 4.49LEE214 pKa = 4.4EE215 pKa = 4.25QAAHH219 pKa = 5.73YY220 pKa = 10.51HH221 pKa = 7.21DD222 pKa = 5.91DD223 pKa = 4.67DD224 pKa = 4.55EE225 pKa = 7.21LILPVKK231 pKa = 10.42RR232 pKa = 11.84KK233 pKa = 8.6VVKK236 pKa = 10.38KK237 pKa = 10.36KK238 pKa = 9.74PQALRR243 pKa = 11.84RR244 pKa = 11.84KK245 pKa = 8.41PLPPVGFAGAMAEE258 pKa = 3.92ARR260 pKa = 11.84EE261 pKa = 4.19KK262 pKa = 11.38GDD264 pKa = 3.29LTFTFPVVFMGEE276 pKa = 4.04SDD278 pKa = 4.25EE279 pKa = 5.92DD280 pKa = 3.87DD281 pKa = 3.87TPVWEE286 pKa = 4.37PLPLKK291 pKa = 8.8TLKK294 pKa = 10.12EE295 pKa = 4.02LQSAVRR301 pKa = 11.84TMGPSAPYY309 pKa = 8.48TLQVVDD315 pKa = 4.68MVASQWLTPSDD326 pKa = 2.96WHH328 pKa = 4.57QTARR332 pKa = 11.84ATLSPGDD339 pKa = 3.64YY340 pKa = 10.37VLWRR344 pKa = 11.84TEE346 pKa = 3.81YY347 pKa = 10.21EE348 pKa = 4.29EE349 pKa = 4.39KK350 pKa = 10.59SKK352 pKa = 11.12EE353 pKa = 4.11MVQKK357 pKa = 10.83AAGKK361 pKa = 9.84RR362 pKa = 11.84KK363 pKa = 10.08GKK365 pKa = 10.57VSLDD369 pKa = 3.39MLLGTGQFLSPSSQIKK385 pKa = 10.11LSKK388 pKa = 10.67DD389 pKa = 3.14VLKK392 pKa = 10.93DD393 pKa = 3.24VTTNAVLAWRR403 pKa = 11.84AIPPPGVKK411 pKa = 8.73KK412 pKa = 9.08TVLAGLKK419 pKa = 9.73QGNEE423 pKa = 3.61EE424 pKa = 4.18SYY426 pKa = 9.06EE427 pKa = 4.17TFISRR432 pKa = 11.84LEE434 pKa = 3.95EE435 pKa = 3.46AVYY438 pKa = 11.09RR439 pKa = 11.84MMPRR443 pKa = 11.84GEE445 pKa = 4.33GSDD448 pKa = 3.43ILIKK452 pKa = 10.43QLAWEE457 pKa = 4.39NANSLCQDD465 pKa = 4.72LIRR468 pKa = 11.84PIRR471 pKa = 11.84KK472 pKa = 7.7TGTIQDD478 pKa = 4.17YY479 pKa = 10.95IRR481 pKa = 11.84ACLDD485 pKa = 3.11ASPAVVQGMAYY496 pKa = 9.19AAAMRR501 pKa = 11.84GQKK504 pKa = 10.41YY505 pKa = 7.44STFVKK510 pKa = 8.72QTYY513 pKa = 10.18GGGKK517 pKa = 9.42GGQGAEE523 pKa = 4.15GPVCFSCGKK532 pKa = 8.21TGHH535 pKa = 6.21IRR537 pKa = 11.84KK538 pKa = 9.18DD539 pKa = 3.55CKK541 pKa = 10.58DD542 pKa = 3.31EE543 pKa = 4.57KK544 pKa = 10.47GSKK547 pKa = 9.53RR548 pKa = 11.84APPGLCPRR556 pKa = 11.84CKK558 pKa = 10.06KK559 pKa = 10.1GYY561 pKa = 8.72HH562 pKa = 5.97WKK564 pKa = 10.34SEE566 pKa = 4.25CKK568 pKa = 10.4SKK570 pKa = 10.64FDD572 pKa = 4.67KK573 pKa = 11.01DD574 pKa = 4.08GNPLPPLEE582 pKa = 4.45TNAEE586 pKa = 3.98NSKK589 pKa = 10.66NLL591 pKa = 3.56

Molecular weight: 66.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P10271|PRO_MMTVB Gag-Pro polyprotein OS=Mouse mammary tumor virus (strain BR6) OX=11758 GN=gag-pro PE=1 SV=2
MM1 pKa = 7.81PRR3 pKa = 11.84LQQKK7 pKa = 8.42WLNSRR12 pKa = 11.84EE13 pKa = 4.22CPTLRR18 pKa = 11.84GEE20 pKa = 4.13AAKK23 pKa = 10.9GLFPTKK29 pKa = 10.09DD30 pKa = 3.35DD31 pKa = 4.12PSAHH35 pKa = 6.27KK36 pKa = 10.61RR37 pKa = 11.84MSPSDD42 pKa = 3.69KK43 pKa = 10.73DD44 pKa = 4.22ILILCCKK51 pKa = 10.39LGIALLCLGLLGEE64 pKa = 4.43VAVRR68 pKa = 11.84ARR70 pKa = 11.84RR71 pKa = 11.84ALTLDD76 pKa = 3.59SFNSSSVQDD85 pKa = 3.71YY86 pKa = 11.0NLNDD90 pKa = 3.87SEE92 pKa = 4.64NSTFLLRR99 pKa = 11.84QGPQPTSSYY108 pKa = 10.51KK109 pKa = 9.98PHH111 pKa = 6.17QPCPSEE117 pKa = 3.8IEE119 pKa = 3.7IRR121 pKa = 11.84MLAKK125 pKa = 10.53NYY127 pKa = 10.11IFTNKK132 pKa = 8.61TNPIGRR138 pKa = 11.84LLVTMLRR145 pKa = 11.84NEE147 pKa = 4.39SLPFSTIFTQIQRR160 pKa = 11.84LEE162 pKa = 3.83MGIEE166 pKa = 3.58NRR168 pKa = 11.84KK169 pKa = 8.49RR170 pKa = 11.84HH171 pKa = 4.71STSVEE176 pKa = 3.78EE177 pKa = 4.14QVQGLRR183 pKa = 11.84ASGLEE188 pKa = 3.87VKK190 pKa = 10.29RR191 pKa = 11.84GKK193 pKa = 10.39RR194 pKa = 11.84SALVKK199 pKa = 10.52IGDD202 pKa = 3.93RR203 pKa = 11.84WWQPGTYY210 pKa = 8.74RR211 pKa = 11.84GPYY214 pKa = 9.15IYY216 pKa = 10.27RR217 pKa = 11.84PTDD220 pKa = 3.06APLPYY225 pKa = 9.61TGRR228 pKa = 11.84YY229 pKa = 9.07DD230 pKa = 4.33LNFDD234 pKa = 3.01RR235 pKa = 11.84WVTVNGYY242 pKa = 9.47KK243 pKa = 10.13VLYY246 pKa = 9.47RR247 pKa = 11.84SLSFRR252 pKa = 11.84EE253 pKa = 3.6RR254 pKa = 11.84LARR257 pKa = 11.84ARR259 pKa = 11.84PPWCMLTQEE268 pKa = 5.24EE269 pKa = 4.81KK270 pKa = 11.18NDD272 pKa = 3.74MKK274 pKa = 11.01QQVHH278 pKa = 6.94DD279 pKa = 4.61YY280 pKa = 9.71IYY282 pKa = 10.4LGTGMSSIWGKK293 pKa = 10.03IFHH296 pKa = 6.08TKK298 pKa = 9.09EE299 pKa = 3.56RR300 pKa = 11.84TVAALIEE307 pKa = 4.6HH308 pKa = 6.62YY309 pKa = 10.01SAKK312 pKa = 9.69TYY314 pKa = 10.38GMSYY318 pKa = 11.1YY319 pKa = 10.7DD320 pKa = 3.32

Molecular weight: 36.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

4214

320

1755

842.8

94.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.431 ± 0.246

1.305 ± 0.11

5.719 ± 0.253

6.645 ± 0.661

3.037 ± 0.322

6.977 ± 0.494

2.373 ± 0.285

4.96 ± 0.404

7.997 ± 0.611

10.18 ± 0.351

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.898 ± 0.123

2.943 ± 0.414

6.455 ± 0.227

4.699 ± 0.355

5.031 ± 0.423

6.906 ± 0.26

5.79 ± 0.229

5.79 ± 0.209

2.065 ± 0.058

2.8 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski