Actinocorallia herbida
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8804 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N1CUK6|A0A3N1CUK6_9ACTN Uncharacterized protein (TIGR03086 family) OS=Actinocorallia herbida OX=58109 GN=EDD29_1911 PE=4 SV=1
MM1 pKa = 7.76 SEE3 pKa = 3.88 VTEE6 pKa = 4.76 PYY8 pKa = 9.67 ATGTPCWVDD17 pKa = 5.27 LMAKK21 pKa = 9.66 DD22 pKa = 3.44 QQAALDD28 pKa = 4.32 FYY30 pKa = 11.44 RR31 pKa = 11.84 EE32 pKa = 3.89 LLGWQGEE39 pKa = 4.15 IGPAEE44 pKa = 3.91 FGGYY48 pKa = 7.0 TVCEE52 pKa = 4.03 LNGRR56 pKa = 11.84 AVAGIAPTMAPEE68 pKa = 4.73 GASEE72 pKa = 4.62 PPTVWTNYY80 pKa = 9.97 LSSTDD85 pKa = 3.47 AQATQDD91 pKa = 4.1 AIVAAGGLLIAPVMDD106 pKa = 3.62 VGSLGRR112 pKa = 11.84 MLVAADD118 pKa = 3.47 PQGAVFGVWEE128 pKa = 4.23 ARR130 pKa = 11.84 DD131 pKa = 3.4 FAGAQVVNEE140 pKa = 4.15 AGALVWNEE148 pKa = 3.96 LHH150 pKa = 6.67 TDD152 pKa = 4.84 DD153 pKa = 5.53 IPGATAFYY161 pKa = 11.25 AEE163 pKa = 4.67 AFDD166 pKa = 5.55 LRR168 pKa = 11.84 IKK170 pKa = 10.4 PMEE173 pKa = 4.3 GAEE176 pKa = 4.52 PYY178 pKa = 10.52 CEE180 pKa = 4.56 LRR182 pKa = 11.84 VGDD185 pKa = 3.86 QCVGGATHH193 pKa = 7.61 LAADD197 pKa = 3.94 PPGTPSHH204 pKa = 6.55 WLTYY208 pKa = 9.87 FAVDD212 pKa = 4.62 DD213 pKa = 3.99 VDD215 pKa = 5.01 ASVDD219 pKa = 3.4 TLLEE223 pKa = 4.23 HH224 pKa = 6.91 GGTVLAPPFDD234 pKa = 4.49 MIAGRR239 pKa = 11.84 MTVVTDD245 pKa = 3.61 PQGAPFALINPTPMM259 pKa = 4.56
Molecular weight: 27.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.884
IPC_protein 3.872
Toseland 3.668
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.567
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.177
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.986
Patrickios 1.138
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.867
Protein with the highest isoelectric point:
>tr|A0A3N1DB48|A0A3N1DB48_9ACTN Aryl-alcohol dehydrogenase-like predicted oxidoreductase OS=Actinocorallia herbida OX=58109 GN=EDD29_8491 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 HH14 pKa = 5.34 KK15 pKa = 10.47 KK16 pKa = 8.84 HH17 pKa = 5.5 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGQAILAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.43 GRR40 pKa = 11.84 ARR42 pKa = 11.84 VAVV45 pKa = 3.65
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8804
0
8804
2882551
29
6424
327.4
34.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.997 ± 0.038
0.767 ± 0.007
5.932 ± 0.021
5.746 ± 0.023
2.97 ± 0.014
9.506 ± 0.023
2.2 ± 0.013
3.443 ± 0.02
2.068 ± 0.02
10.743 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.723 ± 0.012
1.64 ± 0.014
6.213 ± 0.027
2.379 ± 0.014
7.961 ± 0.028
4.85 ± 0.019
5.861 ± 0.021
8.472 ± 0.025
1.518 ± 0.011
2.009 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here