Amino acid dipepetide frequency for Variovorax sp. PBL-E5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.105AlaAla: 19.105 ± 0.133	1.396AlaCys: 1.396 ± 0.027	6.615AlaAsp: 6.615 ± 0.063	6.922AlaGlu: 6.922 ± 0.07	4.457AlaPhe: 4.457 ± 0.048	11.198AlaGly: 11.198 ± 0.085	2.846AlaHis: 2.846 ± 0.039	5.51AlaIle: 5.51 ± 0.054	3.885AlaLys: 3.885 ± 0.056	15.198AlaLeu: 15.198 ± 0.11	3.721AlaMet: 3.721 ± 0.042	2.77AlaAsn: 2.77 ± 0.042	6.566AlaPro: 6.566 ± 0.076	5.538AlaGln: 5.538 ± 0.055	9.742AlaArg: 9.742 ± 0.081	7.263AlaSer: 7.263 ± 0.067	6.161AlaThr: 6.161 ± 0.059	9.271AlaVal: 9.271 ± 0.063	1.855AlaTrp: 1.855 ± 0.035	2.541AlaTyr: 2.541 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	1.282CysAla: 1.282 ± 0.025	0.122CysCys: 0.122 ± 0.008	0.579CysAsp: 0.579 ± 0.017	0.593CysGlu: 0.593 ± 0.018	0.351CysPhe: 0.351 ± 0.013	1.028CysGly: 1.028 ± 0.027	0.253CysHis: 0.253 ± 0.011	0.456CysIle: 0.456 ± 0.016	0.239CysLys: 0.239 ± 0.011	0.823CysLeu: 0.823 ± 0.023	0.23CysMet: 0.23 ± 0.011	0.231CysAsn: 0.231 ± 0.011	0.465CysPro: 0.465 ± 0.016	0.25CysGln: 0.25 ± 0.01	0.626CysArg: 0.626 ± 0.017	0.514CysSer: 0.514 ± 0.018	0.541CysThr: 0.541 ± 0.018	0.782CysVal: 0.782 ± 0.02	0.123CysTrp: 0.123 ± 0.008	0.211CysTyr: 0.211 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.83AspAla: 7.83 ± 0.063	0.504AspCys: 0.504 ± 0.016	2.821AspAsp: 2.821 ± 0.039	3.287AspGlu: 3.287 ± 0.04	2.119AspPhe: 2.119 ± 0.031	4.592AspGly: 4.592 ± 0.053	1.125AspHis: 1.125 ± 0.027	2.416AspIle: 2.416 ± 0.038	1.644AspLys: 1.644 ± 0.029	5.221AspLeu: 5.221 ± 0.054	1.229AspMet: 1.229 ± 0.024	1.157AspAsn: 1.157 ± 0.026	2.961AspPro: 2.961 ± 0.038	1.43AspGln: 1.43 ± 0.028	3.471AspArg: 3.471 ± 0.045	2.261AspSer: 2.261 ± 0.033	2.469AspThr: 2.469 ± 0.041	3.997AspVal: 3.997 ± 0.041	0.856AspTrp: 0.856 ± 0.023	1.367AspTyr: 1.367 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	7.165GluAla: 7.165 ± 0.068	0.452GluCys: 0.452 ± 0.015	2.238GluAsp: 2.238 ± 0.037	2.456GluGlu: 2.456 ± 0.041	1.795GluPhe: 1.795 ± 0.03	3.936GluGly: 3.936 ± 0.047	1.306GluHis: 1.306 ± 0.028	2.63GluIle: 2.63 ± 0.034	1.747GluLys: 1.747 ± 0.03	5.9GluLeu: 5.9 ± 0.058	1.311GluMet: 1.311 ± 0.026	1.167GluAsn: 1.167 ± 0.024	2.554GluPro: 2.554 ± 0.038	2.18GluGln: 2.18 ± 0.032	4.788GluArg: 4.788 ± 0.048	2.671GluSer: 2.671 ± 0.036	2.483GluThr: 2.483 ± 0.036	4.025GluVal: 4.025 ± 0.054	0.729GluTrp: 0.729 ± 0.018	0.957GluTyr: 0.957 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	4.558PheAla: 4.558 ± 0.046	0.438PheCys: 0.438 ± 0.013	2.621PheAsp: 2.621 ± 0.034	2.199PheGlu: 2.199 ± 0.031	1.346PhePhe: 1.346 ± 0.029	3.599PheGly: 3.599 ± 0.051	0.775PheHis: 0.775 ± 0.018	1.526PheIle: 1.526 ± 0.031	1.146PheLys: 1.146 ± 0.024	2.992PheLeu: 2.992 ± 0.038	0.803PheMet: 0.803 ± 0.018	1.03PheAsn: 1.03 ± 0.021	1.561PhePro: 1.561 ± 0.027	1.028PheGln: 1.028 ± 0.02	2.039PheArg: 2.039 ± 0.031	2.146PheSer: 2.146 ± 0.033	1.862PheThr: 1.862 ± 0.029	2.931PheVal: 2.931 ± 0.048	0.504PheTrp: 0.504 ± 0.017	0.827PheTyr: 0.827 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	9.858GlyAla: 9.858 ± 0.083	0.912GlyCys: 0.912 ± 0.025	3.997GlyAsp: 3.997 ± 0.045	4.402GlyGlu: 4.402 ± 0.051	3.417GlyPhe: 3.417 ± 0.038	7.164GlyGly: 7.164 ± 0.093	1.952GlyHis: 1.952 ± 0.031	4.289GlyIle: 4.289 ± 0.045	3.18GlyLys: 3.18 ± 0.046	8.724GlyLeu: 8.724 ± 0.077	2.332GlyMet: 2.332 ± 0.04	2.075GlyAsn: 2.075 ± 0.041	3.43GlyPro: 3.43 ± 0.042	3.039GlyGln: 3.039 ± 0.042	5.784GlyArg: 5.784 ± 0.052	4.73GlySer: 4.73 ± 0.057	4.632GlyThr: 4.632 ± 0.057	6.59GlyVal: 6.59 ± 0.057	1.455GlyTrp: 1.455 ± 0.029	2.297GlyTyr: 2.297 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	3.1HisAla: 3.1 ± 0.051	0.301HisCys: 0.301 ± 0.012	1.209HisAsp: 1.209 ± 0.026	1.199HisGlu: 1.199 ± 0.027	0.887HisPhe: 0.887 ± 0.021	2.165HisGly: 2.165 ± 0.036	0.606HisHis: 0.606 ± 0.018	0.913HisIle: 0.913 ± 0.019	0.54HisLys: 0.54 ± 0.014	2.253HisLeu: 2.253 ± 0.033	0.524HisMet: 0.524 ± 0.015	0.484HisAsn: 0.484 ± 0.017	1.514HisPro: 1.514 ± 0.029	0.667HisGln: 0.667 ± 0.018	1.688HisArg: 1.688 ± 0.031	1.038HisSer: 1.038 ± 0.021	1.002HisThr: 1.002 ± 0.023	1.726HisVal: 1.726 ± 0.025	0.418HisTrp: 0.418 ± 0.016	0.611HisTyr: 0.611 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	6.7IleAla: 6.7 ± 0.057	0.426IleCys: 0.426 ± 0.015	3.256IleAsp: 3.256 ± 0.04	3.178IleGlu: 3.178 ± 0.041	1.263IlePhe: 1.263 ± 0.026	4.492IleGly: 4.492 ± 0.054	0.892IleHis: 0.892 ± 0.02	1.358IleIle: 1.358 ± 0.03	1.324IleLys: 1.324 ± 0.03	3.217IleLeu: 3.217 ± 0.045	0.706IleMet: 0.706 ± 0.018	1.243IleAsn: 1.243 ± 0.029	2.063IlePro: 2.063 ± 0.032	1.201IleGln: 1.201 ± 0.024	2.731IleArg: 2.731 ± 0.038	2.302IleSer: 2.302 ± 0.033	2.28IleThr: 2.28 ± 0.037	3.816IleVal: 3.816 ± 0.048	0.479IleTrp: 0.479 ± 0.015	0.961IleTyr: 0.961 ± 0.022	0.0IleXaa: 0.0 ± 0.0
Lys	4.098LysAla: 4.098 ± 0.058	0.171LysCys: 0.171 ± 0.01	1.638LysAsp: 1.638 ± 0.031	1.512LysGlu: 1.512 ± 0.025	0.92LysPhe: 0.92 ± 0.022	2.372LysGly: 2.372 ± 0.038	0.62LysHis: 0.62 ± 0.015	1.418LysIle: 1.418 ± 0.026	1.381LysLys: 1.381 ± 0.037	3.489LysLeu: 3.489 ± 0.043	0.721LysMet: 0.721 ± 0.019	0.851LysAsn: 0.851 ± 0.024	2.049LysPro: 2.049 ± 0.037	1.091LysGln: 1.091 ± 0.023	2.146LysArg: 2.146 ± 0.036	1.773LysSer: 1.773 ± 0.032	1.915LysThr: 1.915 ± 0.03	2.496LysVal: 2.496 ± 0.036	0.378LysTrp: 0.378 ± 0.015	0.667LysTyr: 0.667 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	15.053LeuAla: 15.053 ± 0.111	1.111LeuCys: 1.111 ± 0.023	5.861LeuAsp: 5.861 ± 0.054	5.042LeuGlu: 5.042 ± 0.052	3.443LeuPhe: 3.443 ± 0.046	8.839LeuGly: 8.839 ± 0.072	2.421LeuHis: 2.421 ± 0.036	4.295LeuIle: 4.295 ± 0.052	3.372LeuLys: 3.372 ± 0.048	10.854LeuLeu: 10.854 ± 0.098	2.494LeuMet: 2.494 ± 0.033	2.545LeuAsn: 2.545 ± 0.039	5.981LeuPro: 5.981 ± 0.051	3.773LeuGln: 3.773 ± 0.045	7.965LeuArg: 7.965 ± 0.066	6.093LeuSer: 6.093 ± 0.057	5.021LeuThr: 5.021 ± 0.046	7.845LeuVal: 7.845 ± 0.07	1.256LeuTrp: 1.256 ± 0.028	1.982LeuTyr: 1.982 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	3.112MetAla: 3.112 ± 0.039	0.2MetCys: 0.2 ± 0.009	1.082MetAsp: 1.082 ± 0.023	0.994MetGlu: 0.994 ± 0.021	0.73MetPhe: 0.73 ± 0.02	1.836MetGly: 1.836 ± 0.035	0.595MetHis: 0.595 ± 0.017	0.954MetIle: 0.954 ± 0.023	1.079MetLys: 1.079 ± 0.023	2.653MetLeu: 2.653 ± 0.043	0.543MetMet: 0.543 ± 0.016	0.911MetAsn: 0.911 ± 0.02	1.619MetPro: 1.619 ± 0.026	1.084MetGln: 1.084 ± 0.022	1.83MetArg: 1.83 ± 0.031	1.634MetSer: 1.634 ± 0.028	1.659MetThr: 1.659 ± 0.031	1.758MetVal: 1.758 ± 0.034	0.199MetTrp: 0.199 ± 0.009	0.37MetTyr: 0.37 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.165AsnAla: 3.165 ± 0.041	0.258AsnCys: 0.258 ± 0.012	1.225AsnAsp: 1.225 ± 0.026	1.169AsnGlu: 1.169 ± 0.025	0.962AsnPhe: 0.962 ± 0.024	2.149AsnGly: 2.149 ± 0.039	0.483AsnHis: 0.483 ± 0.015	1.122AsnIle: 1.122 ± 0.025	0.768AsnLys: 0.768 ± 0.02	2.451AsnLeu: 2.451 ± 0.037	0.526AsnMet: 0.526 ± 0.016	0.66AsnAsn: 0.66 ± 0.02	1.719AsnPro: 1.719 ± 0.027	0.767AsnGln: 0.767 ± 0.019	1.586AsnArg: 1.586 ± 0.029	1.16AsnSer: 1.16 ± 0.027	1.318AsnThr: 1.318 ± 0.033	1.883AsnVal: 1.883 ± 0.033	0.371AsnTrp: 0.371 ± 0.014	0.645AsnTyr: 0.645 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	7.5ProAla: 7.5 ± 0.076	0.378ProCys: 0.378 ± 0.014	3.239ProAsp: 3.239 ± 0.04	3.267ProGlu: 3.267 ± 0.041	1.879ProPhe: 1.879 ± 0.031	4.731ProGly: 4.731 ± 0.052	1.22ProHis: 1.22 ± 0.025	2.08ProIle: 2.08 ± 0.031	1.606ProLys: 1.606 ± 0.027	5.233ProLeu: 5.233 ± 0.052	1.387ProMet: 1.387 ± 0.026	1.235ProAsn: 1.235 ± 0.026	2.859ProPro: 2.859 ± 0.055	1.958ProGln: 1.958 ± 0.034	3.236ProArg: 3.236 ± 0.041	3.066ProSer: 3.066 ± 0.037	2.635ProThr: 2.635 ± 0.039	4.269ProVal: 4.269 ± 0.048	0.802ProTrp: 0.802 ± 0.019	1.236ProTyr: 1.236 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	5.013GlnAla: 5.013 ± 0.054	0.286GlnCys: 0.286 ± 0.012	1.473GlnAsp: 1.473 ± 0.032	1.38GlnGlu: 1.38 ± 0.03	1.194GlnPhe: 1.194 ± 0.022	2.766GlnGly: 2.766 ± 0.041	0.864GlnHis: 0.864 ± 0.022	1.662GlnIle: 1.662 ± 0.029	1.084GlnLys: 1.084 ± 0.024	3.996GlnLeu: 3.996 ± 0.048	0.926GlnMet: 0.926 ± 0.019	0.768GlnAsn: 0.768 ± 0.018	2.074GlnPro: 2.074 ± 0.033	1.709GlnGln: 1.709 ± 0.041	3.239GlnArg: 3.239 ± 0.042	1.896GlnSer: 1.896 ± 0.031	1.759GlnThr: 1.759 ± 0.03	2.802GlnVal: 2.802 ± 0.039	0.564GlnTrp: 0.564 ± 0.019	0.694GlnTyr: 0.694 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	8.54ArgAla: 8.54 ± 0.065	0.669ArgCys: 0.669 ± 0.019	3.707ArgAsp: 3.707 ± 0.043	4.223ArgGlu: 4.223 ± 0.041	2.921ArgPhe: 2.921 ± 0.044	4.879ArgGly: 4.879 ± 0.052	1.933ArgHis: 1.933 ± 0.034	3.714ArgIle: 3.714 ± 0.04	2.237ArgLys: 2.237 ± 0.029	7.845ArgLeu: 7.845 ± 0.077	2.048ArgMet: 2.048 ± 0.033	1.759ArgAsn: 1.759 ± 0.031	3.522ArgPro: 3.522 ± 0.041	2.717ArgGln: 2.717 ± 0.037	5.538ArgArg: 5.538 ± 0.058	3.85ArgSer: 3.85 ± 0.045	3.519ArgThr: 3.519 ± 0.045	5.176ArgVal: 5.176 ± 0.054	1.194ArgTrp: 1.194 ± 0.026	1.782ArgTyr: 1.782 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	6.701SerAla: 6.701 ± 0.055	0.471SerCys: 0.471 ± 0.017	2.685SerAsp: 2.685 ± 0.036	2.552SerGlu: 2.552 ± 0.035	2.246SerPhe: 2.246 ± 0.033	5.203SerGly: 5.203 ± 0.047	1.22SerHis: 1.22 ± 0.026	2.515SerIle: 2.515 ± 0.041	1.648SerLys: 1.648 ± 0.031	5.595SerLeu: 5.595 ± 0.052	1.418SerMet: 1.418 ± 0.028	1.359SerAsn: 1.359 ± 0.028	3.103SerPro: 3.103 ± 0.039	1.783SerGln: 1.783 ± 0.029	3.642SerArg: 3.642 ± 0.044	3.2SerSer: 3.2 ± 0.049	2.959SerThr: 2.959 ± 0.039	3.985SerVal: 3.985 ± 0.041	0.72SerTrp: 0.72 ± 0.02	1.282SerTyr: 1.282 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	6.07ThrAla: 6.07 ± 0.058	0.416ThrCys: 0.416 ± 0.014	2.465ThrAsp: 2.465 ± 0.035	2.328ThrGlu: 2.328 ± 0.034	1.716ThrPhe: 1.716 ± 0.029	4.695ThrGly: 4.695 ± 0.053	1.217ThrHis: 1.217 ± 0.025	2.049ThrIle: 2.049 ± 0.031	1.262ThrLys: 1.262 ± 0.024	6.152ThrLeu: 6.152 ± 0.059	1.171ThrMet: 1.171 ± 0.027	1.157ThrAsn: 1.157 ± 0.029	3.518ThrPro: 3.518 ± 0.038	1.845ThrGln: 1.845 ± 0.029	3.368ThrArg: 3.368 ± 0.038	2.708ThrSer: 2.708 ± 0.039	2.763ThrThr: 2.763 ± 0.044	4.183ThrVal: 4.183 ± 0.047	0.66ThrTrp: 0.66 ± 0.019	1.059ThrTyr: 1.059 ± 0.021	0.0ThrXaa: 0.0 ± 0.0
Val	9.739ValAla: 9.739 ± 0.073	0.762ValCys: 0.762 ± 0.017	4.209ValAsp: 4.209 ± 0.041	4.107ValGlu: 4.107 ± 0.048	2.812ValPhe: 2.812 ± 0.039	5.585ValGly: 5.585 ± 0.058	1.683ValHis: 1.683 ± 0.031	3.527ValIle: 3.527 ± 0.041	2.398ValLys: 2.398 ± 0.04	8.406ValLeu: 8.406 ± 0.074	1.916ValMet: 1.916 ± 0.034	1.991ValAsn: 1.991 ± 0.036	4.333ValPro: 4.333 ± 0.042	2.697ValGln: 2.697 ± 0.034	5.498ValArg: 5.498 ± 0.053	4.077ValSer: 4.077 ± 0.046	3.938ValThr: 3.938 ± 0.047	6.507ValVal: 6.507 ± 0.064	0.934ValTrp: 0.934 ± 0.022	1.5ValTyr: 1.5 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.343TrpAla: 1.343 ± 0.03	0.158TrpCys: 0.158 ± 0.01	0.608TrpAsp: 0.608 ± 0.017	0.52TrpGlu: 0.52 ± 0.017	0.539TrpPhe: 0.539 ± 0.017	0.975TrpGly: 0.975 ± 0.021	0.402TrpHis: 0.402 ± 0.014	0.678TrpIle: 0.678 ± 0.018	0.502TrpLys: 0.502 ± 0.015	1.883TrpLeu: 1.883 ± 0.036	0.403TrpMet: 0.403 ± 0.014	0.445TrpAsn: 0.445 ± 0.016	0.722TrpPro: 0.722 ± 0.02	0.648TrpGln: 0.648 ± 0.018	1.227TrpArg: 1.227 ± 0.028	0.785TrpSer: 0.785 ± 0.021	0.758TrpThr: 0.758 ± 0.02	0.938TrpVal: 0.938 ± 0.019	0.246TrpTrp: 0.246 ± 0.013	0.312TrpTyr: 0.312 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.629TyrAla: 2.629 ± 0.04	0.266TyrCys: 0.266 ± 0.012	1.231TyrAsp: 1.231 ± 0.022	1.16TyrGlu: 1.16 ± 0.024	0.933TyrPhe: 0.933 ± 0.021	2.054TyrGly: 2.054 ± 0.027	0.434TyrHis: 0.434 ± 0.016	0.775TyrIle: 0.775 ± 0.02	0.663TyrLys: 0.663 ± 0.02	2.365TyrLeu: 2.365 ± 0.035	0.435TyrMet: 0.435 ± 0.015	0.549TyrAsn: 0.549 ± 0.016	1.201TyrPro: 1.201 ± 0.024	0.742TyrGln: 0.742 ± 0.018	1.628TyrArg: 1.628 ± 0.031	1.119TyrSer: 1.119 ± 0.023	1.13TyrThr: 1.13 ± 0.024	1.638TyrVal: 1.638 ± 0.031	0.35TyrTrp: 0.35 ± 0.013	0.543TyrTyr: 0.543 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6867 proteins (2151331 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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