Halorubrum pleomorphic virus 1 (HRPV-1)

Taxonomy: Viruses; Monodnaviria; Trapavirae; Saleviricota; Huolimaviricetes; Haloruvirales; Pleolipoviridae; Alphapleolipovirus; Alphapleolipovirus HRPV1

Average proteome isoelectric point is 5.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|C1JJY2|MATRX_HAPV1 Matrix protein OS=Halorubrum pleomorphic virus 1 OX=634168 GN=ORF3 PE=1 SV=1
MM1 pKa = 7.46SKK3 pKa = 10.65SKK5 pKa = 9.69TPNFDD10 pKa = 3.49DD11 pKa = 5.01MEE13 pKa = 4.51VLDD16 pKa = 4.8DD17 pKa = 4.56TNDD20 pKa = 3.48EE21 pKa = 4.27YY22 pKa = 11.72DD23 pKa = 3.73DD24 pKa = 5.74SEE26 pKa = 5.18SEE28 pKa = 4.37WIDD31 pKa = 3.44LDD33 pKa = 3.65RR34 pKa = 11.84GEE36 pKa = 4.59SVVGEE41 pKa = 3.87IRR43 pKa = 11.84EE44 pKa = 4.14INPDD48 pKa = 3.45CGDD51 pKa = 3.42YY52 pKa = 10.0GTTVLEE58 pKa = 4.36LSRR61 pKa = 11.84GLGDD65 pKa = 4.37NVCMWSNRR73 pKa = 11.84QIDD76 pKa = 3.98NKK78 pKa = 10.26IEE80 pKa = 3.89QHH82 pKa = 5.47GLGVGEE88 pKa = 4.13VVGIKK93 pKa = 8.71HH94 pKa = 6.24TDD96 pKa = 3.32RR97 pKa = 11.84EE98 pKa = 4.49QTFTPDD104 pKa = 3.71GSDD107 pKa = 3.28EE108 pKa = 4.02PVKK111 pKa = 10.73FDD113 pKa = 3.47VYY115 pKa = 9.96EE116 pKa = 4.14VRR118 pKa = 11.84AVNVGGNDD126 pKa = 3.13

Molecular weight: 14.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|C1JJY8|ORF9_HAPV1 Uncharacterized protein 9 OS=Halorubrum pleomorphic virus 1 OX=634168 GN=ORF9 PE=4 SV=1
MM1 pKa = 7.56GGGGPPARR9 pKa = 11.84VQGTEE14 pKa = 3.89GSQTGGGAVAVSYY27 pKa = 10.78HH28 pKa = 6.58PNNLDD33 pKa = 3.68TKK35 pKa = 10.02PLSCLPILSNFLLISSNSSLTSSTSSPLLMSSRR68 pKa = 11.84YY69 pKa = 9.59ASAVEE74 pKa = 4.37IPSSSAYY81 pKa = 10.56SSTNSIVASLSRR93 pKa = 11.84IDD95 pKa = 3.52TTSFEE100 pKa = 4.57LLMVSRR106 pKa = 11.84LRR108 pKa = 11.84SSTPGKK114 pKa = 9.06PFCSVPSVAA123 pKa = 5.59

Molecular weight: 12.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

2250

57

518

250.0

27.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.622 ± 0.787

0.667 ± 0.149

7.378 ± 0.768

6.578 ± 0.864

2.933 ± 0.409

7.022 ± 0.464

1.733 ± 0.65

4.267 ± 0.593

3.422 ± 0.479

9.6 ± 1.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.444 ± 0.393

4.489 ± 0.614

3.956 ± 0.426

3.022 ± 0.42

4.978 ± 0.663

10.044 ± 0.988

6.978 ± 0.664

6.889 ± 0.984

1.778 ± 0.3

3.2 ± 0.603
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski