Amino acid dipepetide frequency for Escherichia phage mEpX2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.675AlaAla: 9.675 ± 1.423
0.834AlaCys: 0.834 ± 0.294
6.589AlaAsp: 6.589 ± 0.827
5.922AlaGlu: 5.922 ± 0.743
3.253AlaPhe: 3.253 ± 0.509
7.673AlaGly: 7.673 ± 0.807
1.501AlaHis: 1.501 ± 0.458
7.84AlaIle: 7.84 ± 0.658
4.837AlaLys: 4.837 ± 0.728
6.672AlaLeu: 6.672 ± 0.546
3.67AlaMet: 3.67 ± 0.513
4.087AlaAsn: 4.087 ± 0.447
2.002AlaPro: 2.002 ± 0.369
4.17AlaGln: 4.17 ± 0.573
4.254AlaArg: 4.254 ± 0.685
6.505AlaSer: 6.505 ± 0.856
4.17AlaThr: 4.17 ± 0.635
6.505AlaVal: 6.505 ± 0.715
1.751AlaTrp: 1.751 ± 0.419
2.752AlaTyr: 2.752 ± 0.533
0.0AlaXaa: 0.0 ± 0.0
Cys
1.251CysAla: 1.251 ± 0.378
0.25CysCys: 0.25 ± 0.136
1.001CysAsp: 1.001 ± 0.249
0.751CysGlu: 0.751 ± 0.255
0.083CysPhe: 0.083 ± 0.074
0.834CysGly: 0.834 ± 0.297
0.334CysHis: 0.334 ± 0.197
1.001CysIle: 1.001 ± 0.3
0.917CysLys: 0.917 ± 0.284
1.084CysLeu: 1.084 ± 0.277
0.25CysMet: 0.25 ± 0.167
0.417CysAsn: 0.417 ± 0.167
0.417CysPro: 0.417 ± 0.209
0.417CysGln: 0.417 ± 0.182
0.5CysArg: 0.5 ± 0.218
1.001CysSer: 1.001 ± 0.252
0.25CysThr: 0.25 ± 0.119
0.751CysVal: 0.751 ± 0.242
0.167CysTrp: 0.167 ± 0.18
0.083CysTyr: 0.083 ± 0.081
0.0CysXaa: 0.0 ± 0.0
Asp
6.589AspAla: 6.589 ± 0.886
0.917AspCys: 0.917 ± 0.274
3.837AspAsp: 3.837 ± 0.447
3.086AspGlu: 3.086 ± 0.51
2.669AspPhe: 2.669 ± 0.487
5.421AspGly: 5.421 ± 0.693
0.5AspHis: 0.5 ± 0.243
2.669AspIle: 2.669 ± 0.452
3.086AspLys: 3.086 ± 0.558
5.088AspLeu: 5.088 ± 0.809
1.751AspMet: 1.751 ± 0.351
2.919AspAsn: 2.919 ± 0.433
2.335AspPro: 2.335 ± 0.361
1.835AspGln: 1.835 ± 0.393
3.67AspArg: 3.67 ± 0.616
3.753AspSer: 3.753 ± 0.567
2.002AspThr: 2.002 ± 0.505
4.17AspVal: 4.17 ± 0.609
1.084AspTrp: 1.084 ± 0.331
2.168AspTyr: 2.168 ± 0.437
0.0AspXaa: 0.0 ± 0.0
Glu
5.088GluAla: 5.088 ± 0.588
0.917GluCys: 0.917 ± 0.283
2.335GluAsp: 2.335 ± 0.38
3.92GluGlu: 3.92 ± 0.729
2.085GluPhe: 2.085 ± 0.426
3.336GluGly: 3.336 ± 0.594
0.834GluHis: 0.834 ± 0.255
3.42GluIle: 3.42 ± 0.465
3.586GluLys: 3.586 ± 0.534
5.088GluLeu: 5.088 ± 0.645
1.668GluMet: 1.668 ± 0.386
3.169GluAsn: 3.169 ± 0.462
1.835GluPro: 1.835 ± 0.325
3.753GluGln: 3.753 ± 0.594
4.087GluArg: 4.087 ± 0.784
3.92GluSer: 3.92 ± 0.571
3.336GluThr: 3.336 ± 0.429
4.003GluVal: 4.003 ± 0.646
0.834GluTrp: 0.834 ± 0.339
1.585GluTyr: 1.585 ± 0.371
0.0GluXaa: 0.0 ± 0.0
Phe
3.503PheAla: 3.503 ± 0.508
0.334PheCys: 0.334 ± 0.144
2.836PheAsp: 2.836 ± 0.505
1.835PheGlu: 1.835 ± 0.408
0.834PhePhe: 0.834 ± 0.273
2.919PheGly: 2.919 ± 0.487
0.5PheHis: 0.5 ± 0.2
2.002PheIle: 2.002 ± 0.562
1.751PheLys: 1.751 ± 0.341
1.334PheLeu: 1.334 ± 0.331
0.917PheMet: 0.917 ± 0.268
1.418PheAsn: 1.418 ± 0.336
1.334PhePro: 1.334 ± 0.29
1.001PheGln: 1.001 ± 0.318
1.835PheArg: 1.835 ± 0.525
2.335PheSer: 2.335 ± 0.504
3.086PheThr: 3.086 ± 0.534
1.835PheVal: 1.835 ± 0.323
0.667PheTrp: 0.667 ± 0.181
1.001PheTyr: 1.001 ± 0.292
0.0PheXaa: 0.0 ± 0.0
Gly
6.088GlyAla: 6.088 ± 0.823
0.834GlyCys: 0.834 ± 0.279
4.671GlyAsp: 4.671 ± 0.61
4.337GlyGlu: 4.337 ± 0.6
3.086GlyPhe: 3.086 ± 0.461
5.922GlyGly: 5.922 ± 0.805
1.168GlyHis: 1.168 ± 0.319
4.671GlyIle: 4.671 ± 0.541
5.421GlyLys: 5.421 ± 0.631
5.755GlyLeu: 5.755 ± 0.854
2.919GlyMet: 2.919 ± 0.381
4.17GlyAsn: 4.17 ± 0.738
1.084GlyPro: 1.084 ± 0.345
3.253GlyGln: 3.253 ± 0.507
4.754GlyArg: 4.754 ± 0.533
4.087GlySer: 4.087 ± 0.574
6.088GlyThr: 6.088 ± 1.108
5.505GlyVal: 5.505 ± 0.711
1.084GlyTrp: 1.084 ± 0.339
2.085GlyTyr: 2.085 ± 0.419
0.0GlyXaa: 0.0 ± 0.0
His
1.751HisAla: 1.751 ± 0.423
0.167HisCys: 0.167 ± 0.12
0.834HisAsp: 0.834 ± 0.233
0.751HisGlu: 0.751 ± 0.25
0.751HisPhe: 0.751 ± 0.32
1.668HisGly: 1.668 ± 0.371
0.584HisHis: 0.584 ± 0.211
0.751HisIle: 0.751 ± 0.301
1.001HisLys: 1.001 ± 0.341
1.334HisLeu: 1.334 ± 0.474
0.167HisMet: 0.167 ± 0.123
0.584HisAsn: 0.584 ± 0.207
0.751HisPro: 0.751 ± 0.272
1.001HisGln: 1.001 ± 0.286
1.501HisArg: 1.501 ± 0.373
1.001HisSer: 1.001 ± 0.359
0.584HisThr: 0.584 ± 0.235
0.584HisVal: 0.584 ± 0.302
0.25HisTrp: 0.25 ± 0.148
0.334HisTyr: 0.334 ± 0.159
0.0HisXaa: 0.0 ± 0.0
Ile
5.671IleAla: 5.671 ± 0.69
0.584IleCys: 0.584 ± 0.233
4.42IleAsp: 4.42 ± 0.711
3.169IleGlu: 3.169 ± 0.506
1.751IlePhe: 1.751 ± 0.353
3.753IleGly: 3.753 ± 0.626
1.251IleHis: 1.251 ± 0.361
3.837IleIle: 3.837 ± 0.59
3.086IleLys: 3.086 ± 0.478
5.004IleLeu: 5.004 ± 0.616
1.334IleMet: 1.334 ± 0.353
3.003IleAsn: 3.003 ± 0.522
2.168IlePro: 2.168 ± 0.328
3.837IleGln: 3.837 ± 0.643
3.253IleArg: 3.253 ± 0.529
4.837IleSer: 4.837 ± 0.645
4.17IleThr: 4.17 ± 0.542
2.669IleVal: 2.669 ± 0.558
0.667IleTrp: 0.667 ± 0.234
1.084IleTyr: 1.084 ± 0.235
0.0IleXaa: 0.0 ± 0.0
Lys
5.588LysAla: 5.588 ± 0.756
0.417LysCys: 0.417 ± 0.21
3.42LysAsp: 3.42 ± 0.573
3.837LysGlu: 3.837 ± 0.632
1.251LysPhe: 1.251 ± 0.348
4.17LysGly: 4.17 ± 0.616
0.667LysHis: 0.667 ± 0.223
2.502LysIle: 2.502 ± 0.501
4.337LysLys: 4.337 ± 0.691
5.088LysLeu: 5.088 ± 0.641
1.835LysMet: 1.835 ± 0.418
2.335LysAsn: 2.335 ± 0.34
2.585LysPro: 2.585 ± 0.505
2.919LysGln: 2.919 ± 0.576
3.837LysArg: 3.837 ± 0.735
4.921LysSer: 4.921 ± 0.618
3.253LysThr: 3.253 ± 0.436
3.92LysVal: 3.92 ± 0.633
1.084LysTrp: 1.084 ± 0.241
1.668LysTyr: 1.668 ± 0.343
0.0LysXaa: 0.0 ± 0.0
Leu
8.173LeuAla: 8.173 ± 0.805
1.001LeuCys: 1.001 ± 0.345
4.504LeuAsp: 4.504 ± 0.465
4.671LeuGlu: 4.671 ± 0.612
2.335LeuPhe: 2.335 ± 0.354
3.837LeuGly: 3.837 ± 0.632
1.084LeuHis: 1.084 ± 0.35
4.087LeuIle: 4.087 ± 0.582
4.17LeuLys: 4.17 ± 0.517
5.171LeuLeu: 5.171 ± 0.636
1.668LeuMet: 1.668 ± 0.304
4.17LeuAsn: 4.17 ± 0.689
3.92LeuPro: 3.92 ± 0.504
3.42LeuGln: 3.42 ± 0.613
5.338LeuArg: 5.338 ± 0.674
6.505LeuSer: 6.505 ± 0.735
5.421LeuThr: 5.421 ± 0.538
4.337LeuVal: 4.337 ± 0.517
0.417LeuTrp: 0.417 ± 0.178
2.252LeuTyr: 2.252 ± 0.498
0.0LeuXaa: 0.0 ± 0.0
Met
2.752MetAla: 2.752 ± 0.473
0.25MetCys: 0.25 ± 0.148
1.334MetAsp: 1.334 ± 0.277
1.835MetGlu: 1.835 ± 0.343
0.751MetPhe: 0.751 ± 0.258
2.085MetGly: 2.085 ± 0.378
0.334MetHis: 0.334 ± 0.147
1.251MetIle: 1.251 ± 0.336
2.669MetLys: 2.669 ± 0.447
2.836MetLeu: 2.836 ± 0.514
1.001MetMet: 1.001 ± 0.268
0.917MetAsn: 0.917 ± 0.252
1.251MetPro: 1.251 ± 0.271
1.418MetGln: 1.418 ± 0.329
1.585MetArg: 1.585 ± 0.273
2.168MetSer: 2.168 ± 0.424
2.002MetThr: 2.002 ± 0.412
1.418MetVal: 1.418 ± 0.283
0.167MetTrp: 0.167 ± 0.116
0.5MetTyr: 0.5 ± 0.196
0.0MetXaa: 0.0 ± 0.0
Asn
4.921AsnAla: 4.921 ± 0.699
0.417AsnCys: 0.417 ± 0.213
2.085AsnAsp: 2.085 ± 0.372
2.502AsnGlu: 2.502 ± 0.472
1.001AsnPhe: 1.001 ± 0.246
5.004AsnGly: 5.004 ± 0.636
1.334AsnHis: 1.334 ± 0.396
3.253AsnIle: 3.253 ± 0.572
2.335AsnLys: 2.335 ± 0.422
2.836AsnLeu: 2.836 ± 0.517
0.667AsnMet: 0.667 ± 0.218
1.918AsnAsn: 1.918 ± 0.397
2.419AsnPro: 2.419 ± 0.455
1.918AsnGln: 1.918 ± 0.319
2.252AsnArg: 2.252 ± 0.477
2.335AsnSer: 2.335 ± 0.651
3.67AsnThr: 3.67 ± 0.469
1.835AsnVal: 1.835 ± 0.441
0.834AsnTrp: 0.834 ± 0.283
1.334AsnTyr: 1.334 ± 0.403
0.0AsnXaa: 0.0 ± 0.0
Pro
3.253ProAla: 3.253 ± 0.452
0.5ProCys: 0.5 ± 0.189
2.168ProAsp: 2.168 ± 0.367
3.003ProGlu: 3.003 ± 0.539
1.835ProPhe: 1.835 ± 0.417
3.169ProGly: 3.169 ± 0.508
0.751ProHis: 0.751 ± 0.234
1.251ProIle: 1.251 ± 0.354
2.502ProLys: 2.502 ± 0.564
2.335ProLeu: 2.335 ± 0.572
1.501ProMet: 1.501 ± 0.432
1.251ProAsn: 1.251 ± 0.297
1.668ProPro: 1.668 ± 0.348
1.585ProGln: 1.585 ± 0.399
2.002ProArg: 2.002 ± 0.372
2.335ProSer: 2.335 ± 0.525
1.751ProThr: 1.751 ± 0.44
2.585ProVal: 2.585 ± 0.424
0.417ProTrp: 0.417 ± 0.239
1.168ProTyr: 1.168 ± 0.274
0.0ProXaa: 0.0 ± 0.0
Gln
5.171GlnAla: 5.171 ± 0.68
0.417GlnCys: 0.417 ± 0.209
1.751GlnAsp: 1.751 ± 0.387
2.585GlnGlu: 2.585 ± 0.359
1.251GlnPhe: 1.251 ± 0.358
2.669GlnGly: 2.669 ± 0.464
0.584GlnHis: 0.584 ± 0.227
3.003GlnIle: 3.003 ± 0.35
3.586GlnLys: 3.586 ± 0.634
3.92GlnLeu: 3.92 ± 0.614
1.418GlnMet: 1.418 ± 0.344
1.668GlnAsn: 1.668 ± 0.39
1.501GlnPro: 1.501 ± 0.276
2.752GlnGln: 2.752 ± 0.457
3.003GlnArg: 3.003 ± 0.504
3.753GlnSer: 3.753 ± 0.578
2.335GlnThr: 2.335 ± 0.533
3.086GlnVal: 3.086 ± 0.424
0.667GlnTrp: 0.667 ± 0.217
1.585GlnTyr: 1.585 ± 0.461
0.0GlnXaa: 0.0 ± 0.0
Arg
4.337ArgAla: 4.337 ± 0.627
1.001ArgCys: 1.001 ± 0.358
4.17ArgAsp: 4.17 ± 0.624
3.586ArgGlu: 3.586 ± 0.655
1.835ArgPhe: 1.835 ± 0.411
3.753ArgGly: 3.753 ± 0.598
1.501ArgHis: 1.501 ± 0.367
3.586ArgIle: 3.586 ± 0.503
4.42ArgLys: 4.42 ± 0.764
5.254ArgLeu: 5.254 ± 0.538
1.835ArgMet: 1.835 ± 0.378
3.003ArgAsn: 3.003 ± 0.384
2.168ArgPro: 2.168 ± 0.496
2.419ArgGln: 2.419 ± 0.389
3.503ArgArg: 3.503 ± 0.615
2.836ArgSer: 2.836 ± 0.418
3.169ArgThr: 3.169 ± 0.428
3.586ArgVal: 3.586 ± 0.724
1.668ArgTrp: 1.668 ± 0.394
2.919ArgTyr: 2.919 ± 0.448
0.0ArgXaa: 0.0 ± 0.0
Ser
5.922SerAla: 5.922 ± 0.82
0.5SerCys: 0.5 ± 0.189
4.587SerAsp: 4.587 ± 0.576
4.003SerGlu: 4.003 ± 0.523
2.252SerPhe: 2.252 ± 0.355
6.839SerGly: 6.839 ± 0.816
0.834SerHis: 0.834 ± 0.283
4.003SerIle: 4.003 ± 0.629
3.003SerLys: 3.003 ± 0.585
5.922SerLeu: 5.922 ± 0.665
1.668SerMet: 1.668 ± 0.392
3.086SerAsn: 3.086 ± 0.443
3.253SerPro: 3.253 ± 0.577
3.67SerGln: 3.67 ± 0.534
4.504SerArg: 4.504 ± 0.624
4.754SerSer: 4.754 ± 1.034
3.586SerThr: 3.586 ± 0.497
4.587SerVal: 4.587 ± 0.9
1.668SerTrp: 1.668 ± 0.28
1.168SerTyr: 1.168 ± 0.322
0.0SerXaa: 0.0 ± 0.0
Thr
6.005ThrAla: 6.005 ± 0.651
0.667ThrCys: 0.667 ± 0.228
2.669ThrAsp: 2.669 ± 0.475
3.42ThrGlu: 3.42 ± 0.575
2.919ThrPhe: 2.919 ± 0.644
5.588ThrGly: 5.588 ± 0.743
0.584ThrHis: 0.584 ± 0.208
3.586ThrIle: 3.586 ± 0.585
2.919ThrLys: 2.919 ± 0.571
4.087ThrLeu: 4.087 ± 0.499
1.168ThrMet: 1.168 ± 0.289
2.419ThrAsn: 2.419 ± 0.436
2.585ThrPro: 2.585 ± 0.567
2.669ThrGln: 2.669 ± 0.457
2.836ThrArg: 2.836 ± 0.39
4.17ThrSer: 4.17 ± 0.543
2.919ThrThr: 2.919 ± 0.53
4.17ThrVal: 4.17 ± 0.675
1.418ThrTrp: 1.418 ± 0.387
1.418ThrTyr: 1.418 ± 0.376
0.0ThrXaa: 0.0 ± 0.0
Val
5.004ValAla: 5.004 ± 0.605
0.751ValCys: 0.751 ± 0.26
3.837ValAsp: 3.837 ± 0.499
3.753ValGlu: 3.753 ± 0.505
2.335ValPhe: 2.335 ± 0.3
4.504ValGly: 4.504 ± 0.713
0.917ValHis: 0.917 ± 0.25
4.42ValIle: 4.42 ± 0.623
4.003ValLys: 4.003 ± 0.487
4.087ValLeu: 4.087 ± 0.488
1.918ValMet: 1.918 ± 0.299
3.086ValAsn: 3.086 ± 0.486
2.085ValPro: 2.085 ± 0.385
2.252ValGln: 2.252 ± 0.423
3.42ValArg: 3.42 ± 0.39
5.505ValSer: 5.505 ± 0.687
3.753ValThr: 3.753 ± 0.657
4.42ValVal: 4.42 ± 0.639
1.334ValTrp: 1.334 ± 0.335
1.251ValTyr: 1.251 ± 0.303
0.0ValXaa: 0.0 ± 0.0
Trp
1.084TrpAla: 1.084 ± 0.248
0.584TrpCys: 0.584 ± 0.247
0.917TrpAsp: 0.917 ± 0.292
0.751TrpGlu: 0.751 ± 0.235
0.417TrpPhe: 0.417 ± 0.167
1.501TrpGly: 1.501 ± 0.339
0.834TrpHis: 0.834 ± 0.227
0.667TrpIle: 0.667 ± 0.246
0.917TrpLys: 0.917 ± 0.267
1.668TrpLeu: 1.668 ± 0.473
0.5TrpMet: 0.5 ± 0.244
0.334TrpAsn: 0.334 ± 0.164
0.417TrpPro: 0.417 ± 0.159
0.834TrpGln: 0.834 ± 0.2
1.501TrpArg: 1.501 ± 0.332
1.001TrpSer: 1.001 ± 0.29
1.084TrpThr: 1.084 ± 0.395
1.251TrpVal: 1.251 ± 0.256
0.5TrpTrp: 0.5 ± 0.238
0.584TrpTyr: 0.584 ± 0.231
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.836TyrAla: 2.836 ± 0.437
0.417TyrCys: 0.417 ± 0.163
1.585TyrAsp: 1.585 ± 0.252
1.084TyrGlu: 1.084 ± 0.267
0.584TyrPhe: 0.584 ± 0.27
2.335TyrGly: 2.335 ± 0.459
0.25TyrHis: 0.25 ± 0.136
1.751TyrIle: 1.751 ± 0.363
1.168TyrLys: 1.168 ± 0.29
1.918TyrLeu: 1.918 ± 0.314
0.584TyrMet: 0.584 ± 0.218
0.917TyrAsn: 0.917 ± 0.269
1.251TyrPro: 1.251 ± 0.354
1.585TyrGln: 1.585 ± 0.288
2.919TyrArg: 2.919 ± 0.418
2.085TyrSer: 2.085 ± 0.511
1.585TyrThr: 1.585 ± 0.359
1.418TyrVal: 1.418 ± 0.325
0.667TyrTrp: 0.667 ± 0.185
0.334TyrTyr: 0.334 ± 0.137
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (11991 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski