Clostridium sp. CAG:433

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1308 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6YBZ9|R6YBZ9_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:433 OX=1262806 GN=BN654_00589 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 5.54NIEE5 pKa = 4.3EE6 pKa = 4.37KK7 pKa = 10.51KK8 pKa = 10.85CEE10 pKa = 4.09CGCDD14 pKa = 3.97KK15 pKa = 10.57EE16 pKa = 5.27HH17 pKa = 7.63CDD19 pKa = 5.27DD20 pKa = 5.65SCDD23 pKa = 3.89CCHH26 pKa = 7.32DD27 pKa = 3.64EE28 pKa = 4.55PMVLEE33 pKa = 4.23MEE35 pKa = 4.51DD36 pKa = 3.61VNGEE40 pKa = 4.12KK41 pKa = 10.97VNVQVVGSFDD51 pKa = 5.78DD52 pKa = 3.56NDD54 pKa = 3.09KK55 pKa = 11.35SYY57 pKa = 11.36AIVSDD62 pKa = 3.98LDD64 pKa = 4.16DD65 pKa = 4.64GEE67 pKa = 4.56SYY69 pKa = 11.29YY70 pKa = 10.31IFEE73 pKa = 4.46IQTTDD78 pKa = 3.17EE79 pKa = 4.34GDD81 pKa = 3.55MLVSIDD87 pKa = 5.73DD88 pKa = 3.52EE89 pKa = 5.23DD90 pKa = 4.51EE91 pKa = 3.98FDD93 pKa = 3.93RR94 pKa = 11.84LCKK97 pKa = 10.34VVEE100 pKa = 4.52SKK102 pKa = 11.01LSEE105 pKa = 4.2NNN107 pKa = 3.11

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6YAK0|R6YAK0_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:433 OX=1262806 GN=BN654_00373 PE=4 SV=1
MM1 pKa = 7.88AEE3 pKa = 4.05FRR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 9.15RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.25VCQLCAGKK18 pKa = 10.32EE19 pKa = 3.84LDD21 pKa = 4.39YY22 pKa = 11.7KK23 pKa = 11.15NADD26 pKa = 3.91DD27 pKa = 3.68LRR29 pKa = 11.84KK30 pKa = 10.32YY31 pKa = 10.58INEE34 pKa = 3.97KK35 pKa = 10.69GKK37 pKa = 9.89ILPRR41 pKa = 11.84RR42 pKa = 11.84VTGTCAKK49 pKa = 9.18HH50 pKa = 4.52QRR52 pKa = 11.84IVATQIKK59 pKa = 8.4RR60 pKa = 11.84ARR62 pKa = 11.84MMAILPFTKK71 pKa = 10.36

Molecular weight: 8.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1308

0

1308

404614

29

5855

309.3

35.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.929 ± 0.058

0.991 ± 0.031

6.432 ± 0.061

7.66 ± 0.098

4.124 ± 0.053

5.2 ± 0.094

1.12 ± 0.026

10.067 ± 0.108

10.612 ± 0.101

8.604 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.045

8.216 ± 0.094

2.196 ± 0.034

1.78 ± 0.036

2.839 ± 0.056

6.608 ± 0.089

5.398 ± 0.144

6.099 ± 0.07

0.483 ± 0.019

5.339 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski