Pontibacillus litoralis JSM 072002

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Pontibacillus; Pontibacillus litoralis

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A5FW96|A0A0A5FW96_9BACI Uncharacterized protein OS=Pontibacillus litoralis JSM 072002 OX=1385512 GN=N784_11110 PE=4 SV=1
MM1 pKa = 6.85ATIVIAYY8 pKa = 10.05ASMSGNTEE16 pKa = 3.6EE17 pKa = 4.15MADD20 pKa = 4.24LIKK23 pKa = 10.85EE24 pKa = 4.16DD25 pKa = 4.58LEE27 pKa = 4.62QAGHH31 pKa = 5.59TVTLEE36 pKa = 4.26EE37 pKa = 4.23IDD39 pKa = 3.62VLEE42 pKa = 4.16AHH44 pKa = 6.57TLTDD48 pKa = 3.34YY49 pKa = 11.54DD50 pKa = 4.42GILIGSYY57 pKa = 7.74TWNDD61 pKa = 2.43GDD63 pKa = 4.34LPYY66 pKa = 10.47EE67 pKa = 4.1AEE69 pKa = 4.2EE70 pKa = 4.48FYY72 pKa = 11.39EE73 pKa = 5.11DD74 pKa = 4.14LAEE77 pKa = 4.5VDD79 pKa = 4.05LTNKK83 pKa = 9.47RR84 pKa = 11.84AAVFGSGDD92 pKa = 3.24TAYY95 pKa = 9.91PLYY98 pKa = 10.64CEE100 pKa = 4.6AVHH103 pKa = 6.13TFEE106 pKa = 5.8KK107 pKa = 10.89QLTQCGATIVQEE119 pKa = 4.08GLKK122 pKa = 9.94IEE124 pKa = 4.23MAPTTDD130 pKa = 3.23EE131 pKa = 5.25DD132 pKa = 4.55YY133 pKa = 11.76DD134 pKa = 4.37NIASFSKK141 pKa = 10.57QFATFFNHH149 pKa = 5.09IQARR153 pKa = 11.84SS154 pKa = 3.16

Molecular weight: 17.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A5G1D5|A0A0A5G1D5_9BACI HTH merR-type domain-containing protein OS=Pontibacillus litoralis JSM 072002 OX=1385512 GN=N784_11625 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSDD24 pKa = 3.33PNGRR28 pKa = 11.84QVLQRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.93GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3202

0

3202

917159

27

1791

286.4

32.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.883 ± 0.046

0.718 ± 0.014

5.125 ± 0.036

7.632 ± 0.053

4.312 ± 0.036

6.579 ± 0.041

2.423 ± 0.028

7.656 ± 0.05

6.467 ± 0.041

9.3 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.033 ± 0.022

4.351 ± 0.032

3.542 ± 0.025

4.601 ± 0.042

4.025 ± 0.032

5.863 ± 0.035

5.581 ± 0.035

7.237 ± 0.04

1.008 ± 0.018

3.667 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski