Klebsiella phage vB_KpnP_SU552A
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5PVN0|A0A0C5PVN0_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnP_SU552A OX=1610835 GN=SU552A_07 PE=4 SV=1
MM1 pKa = 7.26 VNVFNIIVTSAMLVLGNDD19 pKa = 3.45 VNNPVPYY26 pKa = 9.36 CTVQLQQPATQEE38 pKa = 3.83 PQPRR42 pKa = 11.84 PEE44 pKa = 3.92 YY45 pKa = 10.91 DD46 pKa = 3.21 LFEE49 pKa = 6.24 DD50 pKa = 4.38 PEE52 pKa = 4.87 GGCKK56 pKa = 9.76 EE57 pKa = 3.82 LGARR61 pKa = 11.84 ILAAVQEE68 pKa = 4.41 QYY70 pKa = 11.3 PDD72 pKa = 3.27 ASVTLTVDD80 pKa = 3.36 GNSNNDD86 pKa = 2.68 II87 pKa = 4.18
Molecular weight: 9.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.777
IPC2_protein 3.77
IPC_protein 3.656
Toseland 3.465
ProMoST 3.846
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.605
Rodwell 3.503
Grimsley 3.389
Solomon 3.617
Lehninger 3.567
Nozaki 3.795
DTASelect 3.948
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.783
Patrickios 0.604
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.73
Protein with the highest isoelectric point:
>tr|A0A0C5PII3|A0A0C5PII3_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnP_SU552A OX=1610835 GN=SU552A_26 PE=4 SV=1
MM1 pKa = 6.8 TVQVISKK8 pKa = 10.09 VCDD11 pKa = 3.4 CGKK14 pKa = 10.54 GYY16 pKa = 10.76 RR17 pKa = 11.84 SAHH20 pKa = 5.89 DD21 pKa = 4.61 LKK23 pKa = 11.05 CGHH26 pKa = 6.6 CRR28 pKa = 11.84 SKK30 pKa = 11.12 QEE32 pKa = 3.92 GAQAGPVPPSARR44 pKa = 11.84 RR45 pKa = 11.84 CKK47 pKa = 10.38 ASAAVRR53 pKa = 11.84 ILSPLRR59 pKa = 11.84 PRR61 pKa = 11.84 RR62 pKa = 3.59
Molecular weight: 6.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.428
IPC_protein 9.706
Toseland 10.57
ProMoST 10.452
Dawson 10.643
Bjellqvist 10.321
Wikipedia 10.789
Rodwell 10.994
Grimsley 10.672
Solomon 10.73
Lehninger 10.716
Nozaki 10.613
DTASelect 10.277
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.584
Patrickios 10.804
IPC_peptide 10.745
IPC2_peptide 9.721
IPC2.peptide.svr19 8.479
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
13741
43
1232
254.5
28.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.654 ± 0.541
1.208 ± 0.193
6.302 ± 0.204
5.771 ± 0.426
3.107 ± 0.155
7.358 ± 0.29
1.776 ± 0.175
4.381 ± 0.173
4.81 ± 0.304
8.798 ± 0.259
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.838 ± 0.216
3.988 ± 0.256
3.69 ± 0.245
4.709 ± 0.353
5.946 ± 0.259
6.557 ± 0.374
5.829 ± 0.355
7.11 ± 0.245
1.426 ± 0.099
3.741 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here