Amino acid dipepetide frequency for Klebsiella sp. RIT-PI-d

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.096AlaAla: 10.096 ± 0.141
1.139AlaCys: 1.139 ± 0.031
5.33AlaAsp: 5.33 ± 0.088
5.579AlaGlu: 5.579 ± 0.088
3.569AlaPhe: 3.569 ± 0.055
7.838AlaGly: 7.838 ± 0.111
1.965AlaHis: 1.965 ± 0.045
6.046AlaIle: 6.046 ± 0.07
3.629AlaLys: 3.629 ± 0.07
11.948AlaLeu: 11.948 ± 0.115
2.88AlaMet: 2.88 ± 0.047
3.036AlaAsn: 3.036 ± 0.054
3.714AlaPro: 3.714 ± 0.06
4.668AlaGln: 4.668 ± 0.08
5.737AlaArg: 5.737 ± 0.088
5.649AlaSer: 5.649 ± 0.078
5.03AlaThr: 5.03 ± 0.085
6.739AlaVal: 6.739 ± 0.083
1.712AlaTrp: 1.712 ± 0.041
1.889AlaTyr: 1.889 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
1.01CysAla: 1.01 ± 0.034
0.169CysCys: 0.169 ± 0.012
0.61CysAsp: 0.61 ± 0.025
0.597CysGlu: 0.597 ± 0.024
0.436CysPhe: 0.436 ± 0.018
1.037CysGly: 1.037 ± 0.031
0.305CysHis: 0.305 ± 0.018
0.554CysIle: 0.554 ± 0.021
0.334CysLys: 0.334 ± 0.017
1.01CysLeu: 1.01 ± 0.033
0.228CysMet: 0.228 ± 0.014
0.322CysAsn: 0.322 ± 0.016
0.511CysPro: 0.511 ± 0.022
0.464CysGln: 0.464 ± 0.018
0.618CysArg: 0.618 ± 0.023
0.655CysSer: 0.655 ± 0.026
0.501CysThr: 0.501 ± 0.021
0.706CysVal: 0.706 ± 0.023
0.195CysTrp: 0.195 ± 0.013
0.335CysTyr: 0.335 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
5.58AspAla: 5.58 ± 0.075
0.534AspCys: 0.534 ± 0.022
3.268AspAsp: 3.268 ± 0.074
3.581AspGlu: 3.581 ± 0.057
2.107AspPhe: 2.107 ± 0.046
3.877AspGly: 3.877 ± 0.079
0.982AspHis: 0.982 ± 0.035
3.68AspIle: 3.68 ± 0.059
2.543AspLys: 2.543 ± 0.049
4.851AspLeu: 4.851 ± 0.079
1.355AspMet: 1.355 ± 0.032
2.359AspAsn: 2.359 ± 0.055
2.329AspPro: 2.329 ± 0.047
1.612AspGln: 1.612 ± 0.037
2.949AspArg: 2.949 ± 0.054
2.858AspSer: 2.858 ± 0.049
2.754AspThr: 2.754 ± 0.064
4.095AspVal: 4.095 ± 0.075
0.806AspTrp: 0.806 ± 0.023
1.877AspTyr: 1.877 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
5.131GluAla: 5.131 ± 0.07
0.449GluCys: 0.449 ± 0.019
2.469GluAsp: 2.469 ± 0.053
3.176GluGlu: 3.176 ± 0.06
1.781GluPhe: 1.781 ± 0.045
3.616GluGly: 3.616 ± 0.056
1.461GluHis: 1.461 ± 0.037
3.301GluIle: 3.301 ± 0.062
3.236GluLys: 3.236 ± 0.064
5.689GluLeu: 5.689 ± 0.082
1.776GluMet: 1.776 ± 0.042
2.288GluAsn: 2.288 ± 0.046
2.045GluPro: 2.045 ± 0.042
3.338GluGln: 3.338 ± 0.061
3.528GluArg: 3.528 ± 0.059
2.949GluSer: 2.949 ± 0.054
2.869GluThr: 2.869 ± 0.052
3.559GluVal: 3.559 ± 0.057
0.764GluTrp: 0.764 ± 0.027
1.392GluTyr: 1.392 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
3.702PheAla: 3.702 ± 0.064
0.532PheCys: 0.532 ± 0.022
2.354PheAsp: 2.354 ± 0.055
1.677PheGlu: 1.677 ± 0.043
1.65PhePhe: 1.65 ± 0.038
3.077PheGly: 3.077 ± 0.054
0.802PheHis: 0.802 ± 0.027
2.648PheIle: 2.648 ± 0.046
1.229PheLys: 1.229 ± 0.034
3.271PheLeu: 3.271 ± 0.056
0.988PheMet: 0.988 ± 0.028
1.756PheAsn: 1.756 ± 0.036
1.503PhePro: 1.503 ± 0.043
1.093PheGln: 1.093 ± 0.031
1.863PheArg: 1.863 ± 0.04
3.14PheSer: 3.14 ± 0.053
2.496PheThr: 2.496 ± 0.059
2.426PheVal: 2.426 ± 0.047
0.615PheTrp: 0.615 ± 0.026
1.194PheTyr: 1.194 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
6.205GlyAla: 6.205 ± 0.082
0.982GlyCys: 0.982 ± 0.03
3.869GlyAsp: 3.869 ± 0.077
4.547GlyGlu: 4.547 ± 0.068
3.219GlyPhe: 3.219 ± 0.059
5.381GlyGly: 5.381 ± 0.086
1.697GlyHis: 1.697 ± 0.04
5.155GlyIle: 5.155 ± 0.07
3.856GlyLys: 3.856 ± 0.067
7.386GlyLeu: 7.386 ± 0.094
2.324GlyMet: 2.324 ± 0.047
2.867GlyAsn: 2.867 ± 0.095
2.064GlyPro: 2.064 ± 0.043
3.006GlyGln: 3.006 ± 0.058
3.757GlyArg: 3.757 ± 0.057
4.168GlySer: 4.168 ± 0.084
3.933GlyThr: 3.933 ± 0.078
5.596GlyVal: 5.596 ± 0.084
1.238GlyTrp: 1.238 ± 0.033
2.583GlyTyr: 2.583 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
1.988HisAla: 1.988 ± 0.046
0.312HisCys: 0.312 ± 0.016
1.277HisAsp: 1.277 ± 0.033
1.075HisGlu: 1.075 ± 0.034
1.096HisPhe: 1.096 ± 0.032
1.739HisGly: 1.739 ± 0.041
0.852HisHis: 0.852 ± 0.032
1.356HisIle: 1.356 ± 0.035
0.792HisLys: 0.792 ± 0.028
2.316HisLeu: 2.316 ± 0.042
0.532HisMet: 0.532 ± 0.02
0.892HisAsn: 0.892 ± 0.027
1.334HisPro: 1.334 ± 0.039
1.196HisGln: 1.196 ± 0.03
1.293HisArg: 1.293 ± 0.033
1.277HisSer: 1.277 ± 0.031
1.142HisThr: 1.142 ± 0.028
1.289HisVal: 1.289 ± 0.035
0.392HisTrp: 0.392 ± 0.018
0.928HisTyr: 0.928 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.665IleAla: 6.665 ± 0.087
0.691IleCys: 0.691 ± 0.022
3.637IleAsp: 3.637 ± 0.059
3.294IleGlu: 3.294 ± 0.066
2.106IlePhe: 2.106 ± 0.049
4.534IleGly: 4.534 ± 0.068
1.225IleHis: 1.225 ± 0.036
3.59IleIle: 3.59 ± 0.072
2.358IleLys: 2.358 ± 0.05
5.204IleLeu: 5.204 ± 0.077
1.385IleMet: 1.385 ± 0.038
2.775IleAsn: 2.775 ± 0.066
2.835IlePro: 2.835 ± 0.051
1.858IleGln: 1.858 ± 0.04
3.036IleArg: 3.036 ± 0.058
3.935IleSer: 3.935 ± 0.067
3.617IleThr: 3.617 ± 0.059
4.175IleVal: 4.175 ± 0.062
0.73IleTrp: 0.73 ± 0.025
1.583IleTyr: 1.583 ± 0.04
0.0IleXaa: 0.0 ± 0.0
Lys
4.147LysAla: 4.147 ± 0.068
0.277LysCys: 0.277 ± 0.016
2.068LysAsp: 2.068 ± 0.052
2.368LysGlu: 2.368 ± 0.05
1.136LysPhe: 1.136 ± 0.037
2.868LysGly: 2.868 ± 0.051
0.823LysHis: 0.823 ± 0.024
2.42LysIle: 2.42 ± 0.05
2.267LysLys: 2.267 ± 0.063
4.087LysLeu: 4.087 ± 0.063
1.261LysMet: 1.261 ± 0.037
1.73LysAsn: 1.73 ± 0.044
2.02LysPro: 2.02 ± 0.041
1.858LysGln: 1.858 ± 0.04
2.475LysArg: 2.475 ± 0.049
2.299LysSer: 2.299 ± 0.051
2.577LysThr: 2.577 ± 0.053
2.889LysVal: 2.889 ± 0.048
0.438LysTrp: 0.438 ± 0.019
1.117LysTyr: 1.117 ± 0.035
0.0LysXaa: 0.0 ± 0.0
Leu
11.415LeuAla: 11.415 ± 0.112
1.268LeuCys: 1.268 ± 0.038
5.545LeuAsp: 5.545 ± 0.079
5.373LeuGlu: 5.373 ± 0.075
4.142LeuPhe: 4.142 ± 0.066
7.047LeuGly: 7.047 ± 0.096
2.383LeuHis: 2.383 ± 0.043
6.039LeuIle: 6.039 ± 0.074
4.434LeuLys: 4.434 ± 0.062
11.815LeuLeu: 11.815 ± 0.16
2.945LeuMet: 2.945 ± 0.045
4.409LeuAsn: 4.409 ± 0.074
5.668LeuPro: 5.668 ± 0.088
4.108LeuGln: 4.108 ± 0.072
6.299LeuArg: 6.299 ± 0.083
7.294LeuSer: 7.294 ± 0.091
6.704LeuThr: 6.704 ± 0.097
7.001LeuVal: 7.001 ± 0.078
1.462LeuTrp: 1.462 ± 0.034
2.596LeuTyr: 2.596 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
2.872MetAla: 2.872 ± 0.054
0.2MetCys: 0.2 ± 0.013
1.201MetAsp: 1.201 ± 0.029
1.208MetGlu: 1.208 ± 0.034
0.848MetPhe: 0.848 ± 0.028
1.828MetGly: 1.828 ± 0.042
0.536MetHis: 0.536 ± 0.021
1.518MetIle: 1.518 ± 0.037
1.346MetLys: 1.346 ± 0.039
3.194MetLeu: 3.194 ± 0.064
0.896MetMet: 0.896 ± 0.027
1.07MetAsn: 1.07 ± 0.029
1.373MetPro: 1.373 ± 0.03
1.23MetGln: 1.23 ± 0.033
1.489MetArg: 1.489 ± 0.034
1.897MetSer: 1.897 ± 0.038
1.78MetThr: 1.78 ± 0.035
1.924MetVal: 1.924 ± 0.041
0.262MetTrp: 0.262 ± 0.017
0.515MetTyr: 0.515 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
3.65AsnAla: 3.65 ± 0.065
0.322AsnCys: 0.322 ± 0.018
2.188AsnAsp: 2.188 ± 0.048
1.925AsnGlu: 1.925 ± 0.038
1.312AsnPhe: 1.312 ± 0.036
3.012AsnGly: 3.012 ± 0.071
0.847AsnHis: 0.847 ± 0.026
2.426AsnIle: 2.426 ± 0.051
1.535AsnLys: 1.535 ± 0.04
3.475AsnLeu: 3.475 ± 0.06
0.954AsnMet: 0.954 ± 0.027
1.687AsnAsn: 1.687 ± 0.054
2.005AsnPro: 2.005 ± 0.047
1.555AsnGln: 1.555 ± 0.041
1.953AsnArg: 1.953 ± 0.045
2.064AsnSer: 2.064 ± 0.05
2.166AsnThr: 2.166 ± 0.058
2.817AsnVal: 2.817 ± 0.067
0.561AsnTrp: 0.561 ± 0.027
1.208AsnTyr: 1.208 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
4.734ProAla: 4.734 ± 0.072
0.359ProCys: 0.359 ± 0.017
2.909ProAsp: 2.909 ± 0.058
3.228ProGlu: 3.228 ± 0.062
1.802ProPhe: 1.802 ± 0.039
3.59ProGly: 3.59 ± 0.052
1.076ProHis: 1.076 ± 0.033
1.739ProIle: 1.739 ± 0.042
1.409ProLys: 1.409 ± 0.036
5.219ProLeu: 5.219 ± 0.085
1.045ProMet: 1.045 ± 0.03
1.121ProAsn: 1.121 ± 0.032
1.679ProPro: 1.679 ± 0.042
2.345ProGln: 2.345 ± 0.049
2.029ProArg: 2.029 ± 0.045
2.111ProSer: 2.111 ± 0.038
2.015ProThr: 2.015 ± 0.04
4.173ProVal: 4.173 ± 0.068
0.796ProTrp: 0.796 ± 0.028
1.12ProTyr: 1.12 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.702GlnAla: 4.702 ± 0.075
0.39GlnCys: 0.39 ± 0.02
1.991GlnAsp: 1.991 ± 0.042
2.149GlnGlu: 2.149 ± 0.044
1.527GlnPhe: 1.527 ± 0.032
3.055GlnGly: 3.055 ± 0.06
1.296GlnHis: 1.296 ± 0.035
2.38GlnIle: 2.38 ± 0.043
1.896GlnLys: 1.896 ± 0.038
4.963GlnLeu: 4.963 ± 0.073
1.204GlnMet: 1.204 ± 0.033
1.516GlnAsn: 1.516 ± 0.042
2.269GlnPro: 2.269 ± 0.062
3.348GlnGln: 3.348 ± 0.077
3.061GlnArg: 3.061 ± 0.056
2.36GlnSer: 2.36 ± 0.048
2.27GlnThr: 2.27 ± 0.053
2.946GlnVal: 2.946 ± 0.052
0.692GlnTrp: 0.692 ± 0.025
1.188GlnTyr: 1.188 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
4.65ArgAla: 4.65 ± 0.07
0.568ArgCys: 0.568 ± 0.025
3.098ArgAsp: 3.098 ± 0.057
3.74ArgGlu: 3.74 ± 0.068
2.56ArgPhe: 2.56 ± 0.044
3.446ArgGly: 3.446 ± 0.065
1.583ArgHis: 1.583 ± 0.039
3.499ArgIle: 3.499 ± 0.055
2.307ArgLys: 2.307 ± 0.049
6.528ArgLeu: 6.528 ± 0.079
1.603ArgMet: 1.603 ± 0.041
1.995ArgAsn: 1.995 ± 0.042
2.27ArgPro: 2.27 ± 0.052
3.251ArgGln: 3.251 ± 0.06
3.556ArgArg: 3.556 ± 0.073
2.766ArgSer: 2.766 ± 0.05
2.556ArgThr: 2.556 ± 0.052
3.79ArgVal: 3.79 ± 0.057
1.016ArgTrp: 1.016 ± 0.03
2.151ArgTyr: 2.151 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
5.876SerAla: 5.876 ± 0.096
0.552SerCys: 0.552 ± 0.025
3.168SerAsp: 3.168 ± 0.064
3.116SerGlu: 3.116 ± 0.056
2.197SerPhe: 2.197 ± 0.041
5.438SerGly: 5.438 ± 0.098
1.476SerHis: 1.476 ± 0.037
3.083SerIle: 3.083 ± 0.056
1.976SerLys: 1.976 ± 0.048
6.664SerLeu: 6.664 ± 0.085
1.456SerMet: 1.456 ± 0.038
1.829SerAsn: 1.829 ± 0.047
2.593SerPro: 2.593 ± 0.047
2.617SerGln: 2.617 ± 0.049
3.541SerArg: 3.541 ± 0.058
3.63SerSer: 3.63 ± 0.058
3.157SerThr: 3.157 ± 0.057
4.377SerVal: 4.377 ± 0.085
0.983SerTrp: 0.983 ± 0.024
1.569SerTyr: 1.569 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
5.267ThrAla: 5.267 ± 0.097
0.537ThrCys: 0.537 ± 0.023
2.777ThrAsp: 2.777 ± 0.06
2.517ThrGlu: 2.517 ± 0.047
2.154ThrPhe: 2.154 ± 0.045
4.667ThrGly: 4.667 ± 0.076
1.289ThrHis: 1.289 ± 0.03
3.045ThrIle: 3.045 ± 0.068
1.503ThrLys: 1.503 ± 0.041
7.868ThrLeu: 7.868 ± 0.108
1.15ThrMet: 1.15 ± 0.029
1.562ThrAsn: 1.562 ± 0.042
3.186ThrPro: 3.186 ± 0.061
2.268ThrGln: 2.268 ± 0.044
3.224ThrArg: 3.224 ± 0.057
3.054ThrSer: 3.054 ± 0.058
2.979ThrThr: 2.979 ± 0.08
4.054ThrVal: 4.054 ± 0.152
0.867ThrTrp: 0.867 ± 0.028
1.149ThrTyr: 1.149 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
6.978ValAla: 6.978 ± 0.083
0.768ValCys: 0.768 ± 0.025
3.957ValAsp: 3.957 ± 0.08
3.857ValGlu: 3.857 ± 0.058
2.564ValPhe: 2.564 ± 0.049
4.725ValGly: 4.725 ± 0.07
1.275ValHis: 1.275 ± 0.036
4.618ValIle: 4.618 ± 0.076
3.094ValLys: 3.094 ± 0.055
7.194ValLeu: 7.194 ± 0.082
2.201ValMet: 2.201 ± 0.049
2.936ValAsn: 2.936 ± 0.053
3.102ValPro: 3.102 ± 0.057
2.523ValGln: 2.523 ± 0.048
3.7ValArg: 3.7 ± 0.06
4.594ValSer: 4.594 ± 0.092
4.377ValThr: 4.377 ± 0.112
5.476ValVal: 5.476 ± 0.071
0.994ValTrp: 0.994 ± 0.037
1.705ValTyr: 1.705 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
1.065TrpAla: 1.065 ± 0.033
0.185TrpCys: 0.185 ± 0.011
0.663TrpAsp: 0.663 ± 0.025
0.604TrpGlu: 0.604 ± 0.022
0.652TrpPhe: 0.652 ± 0.023
0.913TrpGly: 0.913 ± 0.029
0.498TrpHis: 0.498 ± 0.02
0.747TrpIle: 0.747 ± 0.025
0.481TrpLys: 0.481 ± 0.019
2.352TrpLeu: 2.352 ± 0.057
0.444TrpMet: 0.444 ± 0.02
0.5TrpAsn: 0.5 ± 0.022
0.708TrpPro: 0.708 ± 0.027
1.224TrpGln: 1.224 ± 0.037
1.029TrpArg: 1.029 ± 0.027
0.872TrpSer: 0.872 ± 0.032
0.678TrpThr: 0.678 ± 0.03
0.912TrpVal: 0.912 ± 0.028
0.223TrpTrp: 0.223 ± 0.015
0.429TrpTyr: 0.429 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.459TyrAla: 2.459 ± 0.047
0.361TyrCys: 0.361 ± 0.019
1.618TyrAsp: 1.618 ± 0.04
1.205TyrGlu: 1.205 ± 0.032
1.157TyrPhe: 1.157 ± 0.032
2.146TyrGly: 2.146 ± 0.049
0.706TyrHis: 0.706 ± 0.027
1.389TyrIle: 1.389 ± 0.039
0.901TyrLys: 0.901 ± 0.028
2.914TyrLeu: 2.914 ± 0.054
0.558TyrMet: 0.558 ± 0.022
1.015TyrAsn: 1.015 ± 0.037
1.291TyrPro: 1.291 ± 0.035
1.642TyrGln: 1.642 ± 0.039
1.795TyrArg: 1.795 ± 0.04
1.728TyrSer: 1.728 ± 0.043
1.517TyrThr: 1.517 ± 0.059
1.695TyrVal: 1.695 ± 0.041
0.431TyrTrp: 0.431 ± 0.021
0.92TyrTyr: 0.92 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3739 proteins (1233813 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski