Porcine associated porprismacovirus 6

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076VH58|A0A076VH58_9VIRU Rep protein OS=Porcine associated porprismacovirus 6 OX=2170122 PE=4 SV=1
MM1 pKa = 7.13PTGIKK6 pKa = 10.09LALEE10 pKa = 4.28EE11 pKa = 4.47GRR13 pKa = 11.84PTGTGDD19 pKa = 2.94TGGNPMVTVRR29 pKa = 11.84VSEE32 pKa = 4.36TYY34 pKa = 10.72DD35 pKa = 3.26LSTKK39 pKa = 8.75VGKK42 pKa = 9.33MGLVGIHH49 pKa = 6.31TPTGDD54 pKa = 4.83LIPRR58 pKa = 11.84LWSGFVQQYY67 pKa = 9.99KK68 pKa = 10.08KK69 pKa = 10.86FRR71 pKa = 11.84FVKK74 pKa = 10.14CDD76 pKa = 2.95VAMACASMLPADD88 pKa = 4.26PLQIGVEE95 pKa = 4.22SGSIAPQDD103 pKa = 3.58MFNPILYY110 pKa = 9.79KK111 pKa = 10.56AVSNQSMNNFLGWLQLVGEE130 pKa = 4.48TEE132 pKa = 4.22DD133 pKa = 3.65MVGAFNKK140 pKa = 10.41GSVVDD145 pKa = 3.77VNNPVFKK152 pKa = 10.69SAAQDD157 pKa = 3.56GVDD160 pKa = 2.88IDD162 pKa = 3.95QFQMYY167 pKa = 9.94YY168 pKa = 10.9SLLADD173 pKa = 3.27SDD175 pKa = 3.9GWRR178 pKa = 11.84KK179 pKa = 9.97AMPQSGLTMRR189 pKa = 11.84GLIPLVFQVLSNYY202 pKa = 9.75GAGPQNEE209 pKa = 4.57SDD211 pKa = 3.85SADD214 pKa = 3.42LRR216 pKa = 11.84ISVPALSLGPQGGPPTSAADD236 pKa = 3.29SSSFVLPRR244 pKa = 11.84TMRR247 pKa = 11.84GPAARR252 pKa = 11.84MPAIDD257 pKa = 3.43TAFFSSLAAKK267 pKa = 9.89DD268 pKa = 3.73RR269 pKa = 11.84NVFSAFVQAQEE280 pKa = 4.25TLGNVPPAYY289 pKa = 10.26VGIIVLPPAKK299 pKa = 9.97LDD301 pKa = 3.35QLYY304 pKa = 10.56YY305 pKa = 10.03RR306 pKa = 11.84LKK308 pKa = 8.93VTWTIEE314 pKa = 3.99FTGVRR319 pKa = 11.84SNNDD323 pKa = 3.01LSSWSALANAGNVAYY338 pKa = 10.22GSDD341 pKa = 3.73YY342 pKa = 11.36DD343 pKa = 4.06VQSKK347 pKa = 10.82SMASALGMADD357 pKa = 3.23TAGADD362 pKa = 2.99ITKK365 pKa = 10.13IMEE368 pKa = 4.36GSS370 pKa = 3.18

Molecular weight: 39.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076VH58|A0A076VH58_9VIRU Rep protein OS=Porcine associated porprismacovirus 6 OX=2170122 PE=4 SV=1
MM1 pKa = 6.91KK2 pKa = 9.8TYY4 pKa = 9.96MLTIPRR10 pKa = 11.84EE11 pKa = 3.79RR12 pKa = 11.84ADD14 pKa = 4.56RR15 pKa = 11.84IGTEE19 pKa = 3.92PIIRR23 pKa = 11.84LMRR26 pKa = 11.84YY27 pKa = 8.86IRR29 pKa = 11.84EE30 pKa = 3.98NDD32 pKa = 3.18VKK34 pKa = 10.47KK35 pKa = 9.99WIVAMEE41 pKa = 4.36TGRR44 pKa = 11.84RR45 pKa = 11.84GYY47 pKa = 7.87EE48 pKa = 3.43HH49 pKa = 6.04WQVRR53 pKa = 11.84LQARR57 pKa = 11.84EE58 pKa = 4.23GFSEE62 pKa = 4.17WKK64 pKa = 8.98DD65 pKa = 3.4TEE67 pKa = 4.44VPVLGPNWTIVTEE80 pKa = 4.14KK81 pKa = 10.67QKK83 pKa = 11.34VGVGWIQRR91 pKa = 11.84NIPQAHH97 pKa = 5.73VEE99 pKa = 4.16EE100 pKa = 5.68CSDD103 pKa = 3.01NWEE106 pKa = 4.2YY107 pKa = 10.43EE108 pKa = 4.27AKK110 pKa = 10.26EE111 pKa = 3.95GRR113 pKa = 11.84YY114 pKa = 6.59WASWDD119 pKa = 3.49TVEE122 pKa = 4.17VHH124 pKa = 6.09RR125 pKa = 11.84MRR127 pKa = 11.84WGRR130 pKa = 11.84PRR132 pKa = 11.84WYY134 pKa = 10.17QQALLNKK141 pKa = 9.86LRR143 pKa = 11.84TTTDD147 pKa = 2.96RR148 pKa = 11.84EE149 pKa = 4.18VMVWYY154 pKa = 10.05DD155 pKa = 3.34PEE157 pKa = 4.85GNNGKK162 pKa = 9.23SWLVGHH168 pKa = 7.02LFEE171 pKa = 5.21TGRR174 pKa = 11.84AYY176 pKa = 10.74YY177 pKa = 10.16LPPTLTSVQSMLQTMASLAHH197 pKa = 5.75QDD199 pKa = 3.52RR200 pKa = 11.84EE201 pKa = 4.06KK202 pKa = 10.98GYY204 pKa = 9.13PPKK207 pKa = 10.09PYY209 pKa = 10.45VIIDD213 pKa = 3.76IPRR216 pKa = 11.84TWKK219 pKa = 9.02WSKK222 pKa = 10.19EE223 pKa = 3.98LYY225 pKa = 10.17CAIEE229 pKa = 4.3SIKK232 pKa = 10.82DD233 pKa = 3.66GLIMDD238 pKa = 4.71PRR240 pKa = 11.84YY241 pKa = 9.42SARR244 pKa = 11.84PINIRR249 pKa = 11.84GVKK252 pKa = 10.09VLVLTNEE259 pKa = 4.08MPKK262 pKa = 10.41LDD264 pKa = 4.49ALSEE268 pKa = 4.11DD269 pKa = 2.96RR270 pKa = 11.84WIIEE274 pKa = 4.21NTPMHH279 pKa = 6.64

Molecular weight: 33.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

649

279

370

324.5

36.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.55 ± 1.934

0.616 ± 0.067

5.701 ± 0.697

5.239 ± 2.25

2.619 ± 1.273

7.858 ± 1.421

1.079 ± 0.717

5.239 ± 1.051

4.777 ± 0.641

7.704 ± 0.598

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.16 ± 0.146

4.006 ± 0.282

6.317 ± 0.15

4.16 ± 0.385

5.855 ± 2.078

6.934 ± 2.001

5.855 ± 0.399

7.55 ± 0.495

3.082 ± 1.535

3.698 ± 0.643
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski