Synechococcus phage S-RIP1
Average proteome isoelectric point is 5.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M4NNP5|M4NNP5_9CAUD Uncharacterized protein OS=Synechococcus phage S-RIP1 OX=754041 GN=SWVG_00037 PE=4 SV=1
MM1 pKa = 7.52 LLAPEE6 pKa = 4.17 MTDD9 pKa = 3.25 YY10 pKa = 10.95 EE11 pKa = 4.6 CRR13 pKa = 11.84 VSEE16 pKa = 5.53 LIGTLKK22 pKa = 9.93 PARR25 pKa = 11.84 IEE27 pKa = 3.98 HH28 pKa = 7.02 FINEE32 pKa = 4.13 LEE34 pKa = 4.0 GWGIDD39 pKa = 4.68 SEE41 pKa = 4.44 EE42 pKa = 4.14 QLDD45 pKa = 3.67 DD46 pKa = 4.66 AYY48 pKa = 10.88 YY49 pKa = 9.67 GCYY52 pKa = 9.6 PSVEE56 pKa = 4.33 SFVEE60 pKa = 4.76 DD61 pKa = 3.89 LCEE64 pKa = 3.93 DD65 pKa = 4.33 CYY67 pKa = 11.84 ADD69 pKa = 4.66 VIRR72 pKa = 11.84 SMPTFMQTALDD83 pKa = 4.21 YY84 pKa = 11.42 EE85 pKa = 4.74 LMWHH89 pKa = 6.48 QSFQYY94 pKa = 11.06 DD95 pKa = 3.81 FFTIYY100 pKa = 10.84 DD101 pKa = 4.02 RR102 pKa = 11.84 DD103 pKa = 3.6 SGDD106 pKa = 3.24 YY107 pKa = 11.24 YY108 pKa = 11.11 FFNRR112 pKa = 11.84 NFF114 pKa = 3.24
Molecular weight: 13.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.885
IPC2_protein 3.859
IPC_protein 3.821
Toseland 3.617
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.656
Grimsley 3.528
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.101
Thurlkill 3.668
EMBOSS 3.732
Sillero 3.935
Patrickios 0.108
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.851
Protein with the highest isoelectric point:
>tr|M4NUZ0|M4NUZ0_9CAUD Uncharacterized protein OS=Synechococcus phage S-RIP1 OX=754041 GN=SWVG_00034 PE=4 SV=1
MM1 pKa = 7.44 FIKK4 pKa = 9.66 RR5 pKa = 11.84 TSAAIHH11 pKa = 6.4 CISTDD16 pKa = 3.75 FLHH19 pKa = 6.98 GVVDD23 pKa = 3.91 VMFSNGSVYY32 pKa = 10.24 RR33 pKa = 11.84 YY34 pKa = 10.21 SNVSRR39 pKa = 11.84 RR40 pKa = 11.84 AIANLQLQRR49 pKa = 11.84 NMSLGFWVNNNLLGNKK65 pKa = 8.48 RR66 pKa = 11.84 VKK68 pKa = 9.42 STQTYY73 pKa = 9.23 GAIPAIAA80 pKa = 4.3
Molecular weight: 8.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 10.028
IPC_protein 10.847
Toseland 10.716
ProMoST 10.496
Dawson 10.847
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.038
Grimsley 10.921
Solomon 10.965
Lehninger 10.921
Nozaki 10.701
DTASelect 10.599
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.774
Patrickios 10.833
IPC_peptide 10.965
IPC2_peptide 9.721
IPC2.peptide.svr19 8.12
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
11195
49
1067
203.5
22.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.397 ± 0.499
1.081 ± 0.239
6.137 ± 0.239
6.521 ± 0.355
3.609 ± 0.203
7.11 ± 0.458
1.626 ± 0.24
5.333 ± 0.232
5.208 ± 0.358
7.941 ± 0.343
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.787 ± 0.27
4.618 ± 0.282
4.484 ± 0.34
4.859 ± 0.318
5.065 ± 0.292
6.646 ± 0.344
6.664 ± 0.596
5.94 ± 0.256
1.242 ± 0.177
3.734 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here