Tulip virus X

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8BF53|Q8BF53_9VIRU Movement protein TGBp3 OS=Tulip virus X OX=167132 GN=tgb3 PE=3 SV=1
MM1 pKa = 7.73ALNTAPNPEE10 pKa = 4.24ALAAMTLEE18 pKa = 4.43VSSPAVPTPAEE29 pKa = 4.53LDD31 pKa = 3.66TIAAGLTTLGVPADD45 pKa = 3.9SLISHH50 pKa = 7.39ALALVNACFDD60 pKa = 3.86AGSSQFTTISGPSPTPTITLAQLAGVVKK88 pKa = 10.8VSTTLRR94 pKa = 11.84KK95 pKa = 9.4FCRR98 pKa = 11.84FYY100 pKa = 11.78AKK102 pKa = 10.52LIWNARR108 pKa = 11.84LSRR111 pKa = 11.84NRR113 pKa = 11.84PPAGFARR120 pKa = 11.84AYY122 pKa = 10.77VKK124 pKa = 9.83TGQKK128 pKa = 8.78WAGFDD133 pKa = 4.11FFDD136 pKa = 5.32GLLNPAALEE145 pKa = 3.9PLGGLTRR152 pKa = 11.84EE153 pKa = 4.21PTPDD157 pKa = 3.77EE158 pKa = 3.64ITANEE163 pKa = 4.12TARR166 pKa = 11.84SLGLFEE172 pKa = 6.34SRR174 pKa = 11.84ANSNNLATTSTQFTRR189 pKa = 11.84GQLSNTSPTVQFLPSPEE206 pKa = 4.05DD207 pKa = 3.19

Molecular weight: 21.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8BF55|Q8BF55_9VIRU TGB1 OS=Tulip virus X OX=167132 GN=tgb1 PE=4 SV=1
MM1 pKa = 7.51SGAHH5 pKa = 6.58HH6 pKa = 6.76LTPPTNLGKK15 pKa = 9.58PVLAVSVGLSLALLAYY31 pKa = 7.29TATRR35 pKa = 11.84STLPHH40 pKa = 6.73VGDD43 pKa = 3.95TTHH46 pKa = 6.83SLPHH50 pKa = 6.22GGRR53 pKa = 11.84YY54 pKa = 9.34VDD56 pKa = 3.26GTKK59 pKa = 10.31RR60 pKa = 11.84IDD62 pKa = 3.36YY63 pKa = 8.13FRR65 pKa = 11.84PHH67 pKa = 6.91SPNSNPTYY75 pKa = 10.43PFILLTILILSGLILLLSRR94 pKa = 11.84RR95 pKa = 11.84GPAPARR101 pKa = 11.84CPCCQRR107 pKa = 11.84PHH109 pKa = 6.04

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2001

94

1361

400.2

44.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.796 ± 1.104

1.499 ± 0.51

4.398 ± 0.801

5.147 ± 0.882

4.148 ± 0.6

4.598 ± 0.625

3.748 ± 0.995

4.348 ± 0.361

4.698 ± 0.929

12.144 ± 0.889

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.249 ± 0.231

3.648 ± 0.448

8.246 ± 0.907

3.848 ± 0.69

4.798 ± 0.461

7.196 ± 1.022

8.846 ± 1.034

4.748 ± 0.156

1.049 ± 0.308

2.749 ± 0.588
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski