Dinoroseobacter phage vB_DshS-R5C

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nanhaivirus; Dinoroseobacter virus D5C

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 123 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0DY66|A0A1V0DY66_9CAUD Large packaging protein OS=Dinoroseobacter phage vB_DshS-R5C OX=1965368 GN=vBDshSR5C_23 PE=4 SV=1
MM1 pKa = 7.73FDD3 pKa = 3.42QFQEE7 pKa = 4.35AMDD10 pKa = 4.3HH11 pKa = 6.07EE12 pKa = 5.28FNAQFDD18 pKa = 4.16DD19 pKa = 3.5VRR21 pKa = 11.84EE22 pKa = 4.0RR23 pKa = 11.84FAATEE28 pKa = 3.6LDD30 pKa = 3.92AYY32 pKa = 10.84HH33 pKa = 7.15EE34 pKa = 5.45GYY36 pKa = 10.6CDD38 pKa = 3.4YY39 pKa = 11.46CNACDD44 pKa = 4.04EE45 pKa = 5.1DD46 pKa = 4.85GEE48 pKa = 4.59EE49 pKa = 4.34PLEE52 pKa = 4.07FDD54 pKa = 3.51AWKK57 pKa = 10.74ASLNTPRR64 pKa = 11.84AAPSGWGAVALDD76 pKa = 4.24DD77 pKa = 5.58EE78 pKa = 5.17IPFF81 pKa = 4.24

Molecular weight: 9.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0DY60|A0A1V0DY60_9CAUD Uncharacterized protein OS=Dinoroseobacter phage vB_DshS-R5C OX=1965368 GN=vBDshSR5C_50 PE=4 SV=1
MM1 pKa = 7.41SPAEE5 pKa = 4.04ARR7 pKa = 11.84RR8 pKa = 11.84LARR11 pKa = 11.84IIDD14 pKa = 3.91ANASRR19 pKa = 11.84NPVPFQYY26 pKa = 9.81RR27 pKa = 11.84VRR29 pKa = 11.84TRR31 pKa = 11.84PVGDD35 pKa = 4.04RR36 pKa = 11.84FGAWTTLPGVHH47 pKa = 6.69SSRR50 pKa = 11.84ADD52 pKa = 3.14AEE54 pKa = 4.64KK55 pKa = 10.02IAKK58 pKa = 9.13AHH60 pKa = 6.6RR61 pKa = 11.84SSSLDD66 pKa = 3.27VSITEE71 pKa = 4.23NATDD75 pKa = 4.92GYY77 pKa = 11.63

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

123

0

123

24524

37

1460

199.4

22.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.153 ± 0.409

0.93 ± 0.108

7.332 ± 0.171

6.663 ± 0.244

3.906 ± 0.137

7.813 ± 0.257

2.023 ± 0.141

5.179 ± 0.143

4.518 ± 0.28

7.666 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.842 ± 0.147

3.809 ± 0.17

4.11 ± 0.199

3.45 ± 0.136

6.569 ± 0.231

5.264 ± 0.239

6.292 ± 0.327

6.875 ± 0.143

2.01 ± 0.107

2.597 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski