Muribaculaceae bacterium

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; unclassified Muribaculaceae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2214 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S4HF00|A0A4S4HF00_9BACT DUF4421 domain-containing protein OS=Muribaculaceae bacterium OX=2498093 GN=E5990_01125 PE=4 SV=1
MM1 pKa = 7.23EE2 pKa = 4.17QVQINNNSLAFMALANEE19 pKa = 4.39YY20 pKa = 10.29CQALEE25 pKa = 4.03QARR28 pKa = 11.84DD29 pKa = 3.58EE30 pKa = 4.27YY31 pKa = 11.4GQLAFVNAMLRR42 pKa = 11.84ILPRR46 pKa = 11.84IYY48 pKa = 10.17ISASDD53 pKa = 3.53IYY55 pKa = 11.31VPEE58 pKa = 4.53ALLEE62 pKa = 3.79EE63 pKa = 4.83AYY65 pKa = 9.85IDD67 pKa = 3.7NRR69 pKa = 11.84LDD71 pKa = 3.22EE72 pKa = 5.56DD73 pKa = 4.59YY74 pKa = 11.14YY75 pKa = 11.59DD76 pKa = 3.53SVRR79 pKa = 11.84RR80 pKa = 11.84NIEE83 pKa = 3.89NLLGPDD89 pKa = 3.46DD90 pKa = 4.11TYY92 pKa = 11.97LEE94 pKa = 4.52VFHH97 pKa = 7.98QDD99 pKa = 2.66MKK101 pKa = 11.55YY102 pKa = 10.61SDD104 pKa = 3.76TPIAQSVAEE113 pKa = 4.42GLADD117 pKa = 3.81IYY119 pKa = 11.07QVLYY123 pKa = 11.19NFLDD127 pKa = 3.73SVRR130 pKa = 11.84DD131 pKa = 3.79MPADD135 pKa = 3.21VTMAMLTAVKK145 pKa = 10.23EE146 pKa = 4.26DD147 pKa = 3.41FRR149 pKa = 11.84SYY151 pKa = 9.66WGQILCNVMRR161 pKa = 11.84PLNAIAAGSQIDD173 pKa = 3.51ADD175 pKa = 4.05ADD177 pKa = 3.7VFDD180 pKa = 4.45

Molecular weight: 20.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S4FVV8|A0A4S4FVV8_9BACT IS982 family transposase (Fragment) OS=Muribaculaceae bacterium OX=2498093 GN=E5990_11850 PE=4 SV=1
SS1 pKa = 6.19SGRR4 pKa = 11.84VRR6 pKa = 11.84RR7 pKa = 11.84VMTDD11 pKa = 3.04EE12 pKa = 3.48VRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84IDD18 pKa = 3.15GFIARR23 pKa = 11.84NRR25 pKa = 11.84EE26 pKa = 3.53NVAAGLHH33 pKa = 4.92KK34 pKa = 10.47QQMRR38 pKa = 11.84KK39 pKa = 9.6LDD41 pKa = 3.32MWRR44 pKa = 11.84RR45 pKa = 11.84LQDD48 pKa = 2.93EE49 pKa = 4.75GARR52 pKa = 11.84IAYY55 pKa = 7.66STVCQYY61 pKa = 10.28VRR63 pKa = 11.84ALEE66 pKa = 4.33AAPKK70 pKa = 9.19PQEE73 pKa = 4.37KK74 pKa = 9.03PAKK77 pKa = 9.6AYY79 pKa = 9.31IRR81 pKa = 11.84KK82 pKa = 9.88DD83 pKa = 3.36YY84 pKa = 11.16EE85 pKa = 3.84PGFRR89 pKa = 11.84CEE91 pKa = 4.17FDD93 pKa = 3.02WGVLTLWIGGVRR105 pKa = 11.84RR106 pKa = 11.84RR107 pKa = 11.84LL108 pKa = 3.33

Molecular weight: 12.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2214

0

2214

738216

29

2076

333.4

37.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.088 ± 0.048

1.334 ± 0.02

5.627 ± 0.038

5.659 ± 0.049

4.256 ± 0.032

7.038 ± 0.043

1.975 ± 0.022

7.084 ± 0.052

5.655 ± 0.043

8.75 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.815 ± 0.027

5.164 ± 0.04

4.146 ± 0.029

3.278 ± 0.028

5.02 ± 0.044

6.42 ± 0.039

5.773 ± 0.041

6.672 ± 0.043

1.22 ± 0.022

4.025 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski