Firmicutes bacterium CAG:555

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1723 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5CWD3|R5CWD3_9FIRM Transporter major facilitator family OS=Firmicutes bacterium CAG:555 OX=1263030 GN=BN705_01697 PE=4 SV=1
MM1 pKa = 8.02PDD3 pKa = 3.53YY4 pKa = 11.24KK5 pKa = 11.29SMYY8 pKa = 9.53TLLCKK13 pKa = 10.43AVDD16 pKa = 3.94DD17 pKa = 6.05AIDD20 pKa = 4.15PLSKK24 pKa = 10.04IPEE27 pKa = 4.55AVPVAQVLIIALNAAEE43 pKa = 4.56EE44 pKa = 4.53IYY46 pKa = 10.43IEE48 pKa = 4.44SCDD51 pKa = 3.94DD52 pKa = 3.29

Molecular weight: 5.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5D6Z3|R5D6Z3_9FIRM CRISPR-associated endonuclease Cas1 OS=Firmicutes bacterium CAG:555 OX=1263030 GN=cas1 PE=3 SV=1
MM1 pKa = 7.45TLPPRR6 pKa = 11.84SPPAVDD12 pKa = 4.51IINDD16 pKa = 3.75TRR18 pKa = 11.84PSAIIFSVLQSRR30 pKa = 11.84KK31 pKa = 8.39FCATIVAPTEE41 pKa = 4.05VARR44 pKa = 11.84KK45 pKa = 7.13MVTMFMSAFCAVSEE59 pKa = 4.38SLSVTPHH66 pKa = 4.47SRR68 pKa = 11.84KK69 pKa = 9.52RR70 pKa = 11.84LPSMRR75 pKa = 11.84QPMSGAVEE83 pKa = 4.15GRR85 pKa = 11.84SRR87 pKa = 11.84MTKK90 pKa = 9.92VATTMGKK97 pKa = 8.97MIFSFFEE104 pKa = 3.88TSRR107 pKa = 11.84VCSILTLRR115 pKa = 11.84ICSVVRR121 pKa = 11.84SFMSGGCIIGMSAMYY136 pKa = 9.58EE137 pKa = 3.97YY138 pKa = 11.03AAIAMGPNSCGARR151 pKa = 11.84RR152 pKa = 11.84VAVNIAVGPSAPPMMPIEE170 pKa = 4.36AASGPEE176 pKa = 4.11KK177 pKa = 10.53PMHH180 pKa = 5.62TARR183 pKa = 11.84KK184 pKa = 7.9NAKK187 pKa = 7.81NTPSCAAAPKK197 pKa = 10.4SRR199 pKa = 11.84LLGLASSGPKK209 pKa = 9.86SVIAPTPRR217 pKa = 11.84NISEE221 pKa = 4.1GG222 pKa = 3.57

Molecular weight: 23.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1723

0

1723

521547

29

2406

302.7

33.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.157 ± 0.069

1.924 ± 0.026

5.95 ± 0.052

6.641 ± 0.062

3.963 ± 0.043

7.589 ± 0.05

1.687 ± 0.026

6.727 ± 0.049

6.18 ± 0.048

9.088 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.037 ± 0.032

3.807 ± 0.034

3.621 ± 0.036

2.488 ± 0.03

4.734 ± 0.06

6.025 ± 0.053

5.503 ± 0.062

7.307 ± 0.053

0.89 ± 0.023

3.677 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski