Amino acid dipepetide frequency for Firmicutes bacterium CAG:555

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.789AlaAla: 10.789 ± 0.216	1.601AlaCys: 1.601 ± 0.056	5.909AlaAsp: 5.909 ± 0.156	6.824AlaGlu: 6.824 ± 0.134	3.428AlaPhe: 3.428 ± 0.093	6.546AlaGly: 6.546 ± 0.107	1.35AlaHis: 1.35 ± 0.062	5.583AlaIle: 5.583 ± 0.123	5.597AlaLys: 5.597 ± 0.112	8.812AlaLeu: 8.812 ± 0.171	2.953AlaMet: 2.953 ± 0.083	2.711AlaAsn: 2.711 ± 0.08	2.918AlaPro: 2.918 ± 0.081	2.765AlaGln: 2.765 ± 0.085	3.566AlaArg: 3.566 ± 0.089	4.615AlaSer: 4.615 ± 0.107	3.622AlaThr: 3.622 ± 0.099	8.362AlaVal: 8.362 ± 0.145	0.677AlaTrp: 0.677 ± 0.033	2.936AlaTyr: 2.936 ± 0.078	0.006AlaXaa: 0.006 ± 0.003
Cys	1.816CysAla: 1.816 ± 0.064	0.393CysCys: 0.393 ± 0.03	1.352CysAsp: 1.352 ± 0.056	1.131CysGlu: 1.131 ± 0.048	0.775CysPhe: 0.775 ± 0.038	2.222CysGly: 2.222 ± 0.077	0.293CysHis: 0.293 ± 0.026	1.311CysIle: 1.311 ± 0.054	0.884CysLys: 0.884 ± 0.044	1.369CysLeu: 1.369 ± 0.056	0.454CysMet: 0.454 ± 0.029	0.581CysAsn: 0.581 ± 0.036	0.893CysPro: 0.893 ± 0.044	0.339CysGln: 0.339 ± 0.027	1.033CysArg: 1.033 ± 0.053	1.156CysSer: 1.156 ± 0.052	1.049CysThr: 1.049 ± 0.045	1.428CysVal: 1.428 ± 0.065	0.169CysTrp: 0.169 ± 0.019	0.594CysTyr: 0.594 ± 0.035	0.0CysXaa: 0.0 ± 0.0
Asp	5.355AspAla: 5.355 ± 0.141	1.149AspCys: 1.149 ± 0.048	3.585AspAsp: 3.585 ± 0.086	4.931AspGlu: 4.931 ± 0.102	2.529AspPhe: 2.529 ± 0.068	5.19AspGly: 5.19 ± 0.124	0.83AspHis: 0.83 ± 0.049	4.774AspIle: 4.774 ± 0.098	3.856AspLys: 3.856 ± 0.09	4.27AspLeu: 4.27 ± 0.09	1.998AspMet: 1.998 ± 0.071	2.239AspAsn: 2.239 ± 0.068	2.121AspPro: 2.121 ± 0.052	0.792AspGln: 0.792 ± 0.041	2.665AspArg: 2.665 ± 0.078	2.991AspSer: 2.991 ± 0.082	3.309AspThr: 3.309 ± 0.094	3.66AspVal: 3.66 ± 0.099	0.571AspTrp: 0.571 ± 0.036	2.679AspTyr: 2.679 ± 0.072	0.0AspXaa: 0.0 ± 0.0
Glu	5.733GluAla: 5.733 ± 0.125	1.072GluCys: 1.072 ± 0.048	3.175GluAsp: 3.175 ± 0.084	4.163GluGlu: 4.163 ± 0.119	2.452GluPhe: 2.452 ± 0.077	3.775GluGly: 3.775 ± 0.096	1.57GluHis: 1.57 ± 0.062	4.389GluIle: 4.389 ± 0.101	5.183GluLys: 5.183 ± 0.112	7.635GluLeu: 7.635 ± 0.158	2.119GluMet: 2.119 ± 0.07	3.319GluAsn: 3.319 ± 0.071	2.239GluPro: 2.239 ± 0.076	2.284GluGln: 2.284 ± 0.07	3.676GluArg: 3.676 ± 0.097	3.012GluSer: 3.012 ± 0.077	3.451GluThr: 3.451 ± 0.071	3.996GluVal: 3.996 ± 0.096	0.51GluTrp: 0.51 ± 0.03	2.656GluTyr: 2.656 ± 0.076	0.002GluXaa: 0.002 ± 0.002
Phe	3.733PheAla: 3.733 ± 0.089	0.807PheCys: 0.807 ± 0.034	2.759PheAsp: 2.759 ± 0.064	2.548PheGlu: 2.548 ± 0.068	1.699PhePhe: 1.699 ± 0.061	3.434PheGly: 3.434 ± 0.096	0.543PheHis: 0.543 ± 0.036	2.636PheIle: 2.636 ± 0.083	2.078PheLys: 2.078 ± 0.064	3.152PheLeu: 3.152 ± 0.1	1.162PheMet: 1.162 ± 0.05	1.486PheAsn: 1.486 ± 0.059	1.367PhePro: 1.367 ± 0.039	0.591PheGln: 0.591 ± 0.031	1.559PheArg: 1.559 ± 0.055	2.717PheSer: 2.717 ± 0.07	2.422PheThr: 2.422 ± 0.073	3.152PheVal: 3.152 ± 0.087	0.393PheTrp: 0.393 ± 0.027	1.388PheTyr: 1.388 ± 0.054	0.0PheXaa: 0.0 ± 0.0
Gly	6.304GlyAla: 6.304 ± 0.131	1.547GlyCys: 1.547 ± 0.053	4.291GlyAsp: 4.291 ± 0.101	4.933GlyGlu: 4.933 ± 0.114	3.286GlyPhe: 3.286 ± 0.09	5.744GlyGly: 5.744 ± 0.137	1.331GlyHis: 1.331 ± 0.046	5.789GlyIle: 5.789 ± 0.11	5.601GlyLys: 5.601 ± 0.102	6.32GlyLeu: 6.32 ± 0.121	2.496GlyMet: 2.496 ± 0.077	2.552GlyAsn: 2.552 ± 0.08	1.29GlyPro: 1.29 ± 0.048	1.797GlyGln: 1.797 ± 0.064	3.549GlyArg: 3.549 ± 0.09	4.88GlySer: 4.88 ± 0.109	4.414GlyThr: 4.414 ± 0.132	5.963GlyVal: 5.963 ± 0.105	0.738GlyTrp: 0.738 ± 0.044	3.06GlyTyr: 3.06 ± 0.084	0.004GlyXaa: 0.004 ± 0.003
His	1.21HisAla: 1.21 ± 0.056	0.355HisCys: 0.355 ± 0.028	1.051HisAsp: 1.051 ± 0.048	1.241HisGlu: 1.241 ± 0.056	0.719HisPhe: 0.719 ± 0.037	1.471HisGly: 1.471 ± 0.057	0.351HisHis: 0.351 ± 0.03	1.323HisIle: 1.323 ± 0.049	0.83HisLys: 0.83 ± 0.042	1.29HisLeu: 1.29 ± 0.052	0.458HisMet: 0.458 ± 0.028	0.681HisAsn: 0.681 ± 0.035	0.888HisPro: 0.888 ± 0.041	0.36HisGln: 0.36 ± 0.022	0.893HisArg: 0.893 ± 0.046	0.989HisSer: 0.989 ± 0.045	0.922HisThr: 0.922 ± 0.039	0.943HisVal: 0.943 ± 0.045	0.199HisTrp: 0.199 ± 0.02	0.698HisTyr: 0.698 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	6.467IleAla: 6.467 ± 0.114	1.427IleCys: 1.427 ± 0.055	4.318IleAsp: 4.318 ± 0.112	4.347IleGlu: 4.347 ± 0.098	2.475IlePhe: 2.475 ± 0.078	5.269IleGly: 5.269 ± 0.119	0.936IleHis: 0.936 ± 0.042	4.849IleIle: 4.849 ± 0.108	3.927IleLys: 3.927 ± 0.086	5.395IleLeu: 5.395 ± 0.106	1.839IleMet: 1.839 ± 0.067	2.707IleAsn: 2.707 ± 0.071	2.857IlePro: 2.857 ± 0.077	1.346IleGln: 1.346 ± 0.052	2.78IleArg: 2.78 ± 0.097	4.811IleSer: 4.811 ± 0.095	3.712IleThr: 3.712 ± 0.087	5.211IleVal: 5.211 ± 0.114	0.504IleTrp: 0.504 ± 0.031	2.092IleTyr: 2.092 ± 0.068	0.0IleXaa: 0.0 ± 0.0
Lys	5.831LysAla: 5.831 ± 0.123	0.922LysCys: 0.922 ± 0.051	2.943LysAsp: 2.943 ± 0.084	3.447LysGlu: 3.447 ± 0.094	1.998LysPhe: 1.998 ± 0.062	3.791LysGly: 3.791 ± 0.094	1.133LysHis: 1.133 ± 0.055	3.787LysIle: 3.787 ± 0.085	4.558LysLys: 4.558 ± 0.105	6.408LysLeu: 6.408 ± 0.114	2.055LysMet: 2.055 ± 0.062	2.884LysAsn: 2.884 ± 0.077	2.535LysPro: 2.535 ± 0.076	1.998LysGln: 1.998 ± 0.065	3.087LysArg: 3.087 ± 0.07	3.388LysSer: 3.388 ± 0.08	4.046LysThr: 4.046 ± 0.09	3.931LysVal: 3.931 ± 0.094	0.577LysTrp: 0.577 ± 0.034	2.479LysTyr: 2.479 ± 0.075	0.0LysXaa: 0.0 ± 0.0
Leu	8.22LeuAla: 8.22 ± 0.138	2.138LeuCys: 2.138 ± 0.081	5.568LeuAsp: 5.568 ± 0.121	5.309LeuGlu: 5.309 ± 0.11	3.685LeuPhe: 3.685 ± 0.089	6.862LeuGly: 6.862 ± 0.118	1.57LeuHis: 1.57 ± 0.059	5.775LeuIle: 5.775 ± 0.124	5.426LeuLys: 5.426 ± 0.101	8.408LeuLeu: 8.408 ± 0.17	2.588LeuMet: 2.588 ± 0.071	3.53LeuAsn: 3.53 ± 0.083	3.915LeuPro: 3.915 ± 0.089	1.921LeuGln: 1.921 ± 0.066	4.675LeuArg: 4.675 ± 0.112	6.431LeuSer: 6.431 ± 0.121	5.158LeuThr: 5.158 ± 0.11	5.994LeuVal: 5.994 ± 0.105	0.731LeuTrp: 0.731 ± 0.039	2.978LeuTyr: 2.978 ± 0.069	0.002LeuXaa: 0.002 ± 0.002
Met	2.583MetAla: 2.583 ± 0.074	0.52MetCys: 0.52 ± 0.033	1.678MetAsp: 1.678 ± 0.064	1.695MetGlu: 1.695 ± 0.061	1.127MetPhe: 1.127 ± 0.045	2.146MetGly: 2.146 ± 0.064	0.6MetHis: 0.6 ± 0.034	1.754MetIle: 1.754 ± 0.056	2.065MetLys: 2.065 ± 0.066	3.166MetLeu: 3.166 ± 0.089	0.869MetMet: 0.869 ± 0.043	1.344MetAsn: 1.344 ± 0.064	1.413MetPro: 1.413 ± 0.06	1.047MetGln: 1.047 ± 0.046	1.528MetArg: 1.528 ± 0.055	2.199MetSer: 2.199 ± 0.064	1.731MetThr: 1.731 ± 0.055	1.758MetVal: 1.758 ± 0.068	0.222MetTrp: 0.222 ± 0.023	0.92MetTyr: 0.92 ± 0.043	0.0MetXaa: 0.0 ± 0.0
Asn	3.61AsnAla: 3.61 ± 0.112	0.79AsnCys: 0.79 ± 0.043	2.312AsnAsp: 2.312 ± 0.073	2.447AsnGlu: 2.447 ± 0.078	1.382AsnPhe: 1.382 ± 0.053	3.541AsnGly: 3.541 ± 0.092	0.522AsnHis: 0.522 ± 0.034	2.866AsnIle: 2.866 ± 0.077	2.126AsnLys: 2.126 ± 0.059	2.891AsnLeu: 2.891 ± 0.072	1.139AsnMet: 1.139 ± 0.049	1.465AsnAsn: 1.465 ± 0.062	1.572AsnPro: 1.572 ± 0.049	0.698AsnGln: 0.698 ± 0.031	1.781AsnArg: 1.781 ± 0.07	2.224AsnSer: 2.224 ± 0.076	2.14AsnThr: 2.14 ± 0.072	2.682AsnVal: 2.682 ± 0.07	0.385AsnTrp: 0.385 ± 0.032	1.49AsnTyr: 1.49 ± 0.058	0.0AsnXaa: 0.0 ± 0.0
Pro	3.225ProAla: 3.225 ± 0.09	0.589ProCys: 0.589 ± 0.032	2.688ProAsp: 2.688 ± 0.077	3.975ProGlu: 3.975 ± 0.094	1.434ProPhe: 1.434 ± 0.059	2.301ProGly: 2.301 ± 0.068	0.681ProHis: 0.681 ± 0.033	2.023ProIle: 2.023 ± 0.062	2.132ProLys: 2.132 ± 0.071	2.82ProLeu: 2.82 ± 0.07	0.982ProMet: 0.982 ± 0.047	1.252ProAsn: 1.252 ± 0.059	1.058ProPro: 1.058 ± 0.055	1.085ProGln: 1.085 ± 0.044	1.281ProArg: 1.281 ± 0.054	1.929ProSer: 1.929 ± 0.059	2.061ProThr: 2.061 ± 0.069	3.049ProVal: 3.049 ± 0.077	0.322ProTrp: 0.322 ± 0.028	1.325ProTyr: 1.325 ± 0.051	0.0ProXaa: 0.0 ± 0.0
Gln	2.226GlnAla: 2.226 ± 0.074	0.372GlnCys: 0.372 ± 0.031	1.022GlnAsp: 1.022 ± 0.048	1.415GlnGlu: 1.415 ± 0.055	1.043GlnPhe: 1.043 ± 0.044	1.586GlnGly: 1.586 ± 0.058	0.451GlnHis: 0.451 ± 0.031	1.747GlnIle: 1.747 ± 0.058	1.649GlnLys: 1.649 ± 0.063	2.656GlnLeu: 2.656 ± 0.076	0.882GlnMet: 0.882 ± 0.043	1.035GlnAsn: 1.035 ± 0.046	0.83GlnPro: 0.83 ± 0.041	0.834GlnGln: 0.834 ± 0.043	1.382GlnArg: 1.382 ± 0.049	1.471GlnSer: 1.471 ± 0.052	1.522GlnThr: 1.522 ± 0.056	1.584GlnVal: 1.584 ± 0.054	0.251GlnTrp: 0.251 ± 0.023	0.92GlnTyr: 0.92 ± 0.047	0.0GlnXaa: 0.0 ± 0.0
Arg	3.493ArgAla: 3.493 ± 0.084	0.947ArgCys: 0.947 ± 0.053	2.495ArgAsp: 2.495 ± 0.066	3.223ArgGlu: 3.223 ± 0.082	1.965ArgPhe: 1.965 ± 0.057	2.794ArgGly: 2.794 ± 0.077	0.926ArgHis: 0.926 ± 0.045	3.363ArgIle: 3.363 ± 0.088	3.049ArgLys: 3.049 ± 0.079	4.767ArgLeu: 4.767 ± 0.103	1.528ArgMet: 1.528 ± 0.051	1.705ArgAsn: 1.705 ± 0.059	1.549ArgPro: 1.549 ± 0.066	1.543ArgGln: 1.543 ± 0.05	3.158ArgArg: 3.158 ± 0.099	3.116ArgSer: 3.116 ± 0.08	2.542ArgThr: 2.542 ± 0.067	2.769ArgVal: 2.769 ± 0.07	0.422ArgTrp: 0.422 ± 0.028	1.983ArgTyr: 1.983 ± 0.063	0.006ArgXaa: 0.006 ± 0.003
Ser	5.892SerAla: 5.892 ± 0.108	1.024SerCys: 1.024 ± 0.044	3.695SerAsp: 3.695 ± 0.087	3.965SerGlu: 3.965 ± 0.091	2.746SerPhe: 2.746 ± 0.079	5.543SerGly: 5.543 ± 0.112	0.955SerHis: 0.955 ± 0.043	3.816SerIle: 3.816 ± 0.088	3.112SerLys: 3.112 ± 0.075	5.213SerLeu: 5.213 ± 0.1	1.766SerMet: 1.766 ± 0.066	1.898SerAsn: 1.898 ± 0.069	1.927SerPro: 1.927 ± 0.058	1.363SerGln: 1.363 ± 0.054	2.878SerArg: 2.878 ± 0.071	3.637SerSer: 3.637 ± 0.119	3.024SerThr: 3.024 ± 0.109	4.983SerVal: 4.983 ± 0.112	0.585SerTrp: 0.585 ± 0.038	2.232SerTyr: 2.232 ± 0.079	0.0SerXaa: 0.0 ± 0.0
Thr	5.599ThrAla: 5.599 ± 0.134	0.844ThrCys: 0.844 ± 0.05	3.486ThrAsp: 3.486 ± 0.093	3.725ThrGlu: 3.725 ± 0.088	1.691ThrPhe: 1.691 ± 0.058	4.901ThrGly: 4.901 ± 0.099	0.936ThrHis: 0.936 ± 0.046	3.131ThrIle: 3.131 ± 0.089	2.882ThrLys: 2.882 ± 0.09	5.112ThrLeu: 5.112 ± 0.105	1.496ThrMet: 1.496 ± 0.054	1.929ThrAsn: 1.929 ± 0.07	2.581ThrPro: 2.581 ± 0.084	1.361ThrGln: 1.361 ± 0.044	2.034ThrArg: 2.034 ± 0.061	2.853ThrSer: 2.853 ± 0.088	2.801ThrThr: 2.801 ± 0.094	5.524ThrVal: 5.524 ± 0.13	0.451ThrTrp: 0.451 ± 0.035	1.697ThrTyr: 1.697 ± 0.073	0.0ThrXaa: 0.0 ± 0.0
Val	5.739ValAla: 5.739 ± 0.11	1.775ValCys: 1.775 ± 0.065	4.0ValAsp: 4.0 ± 0.095	4.421ValGlu: 4.421 ± 0.087	3.148ValPhe: 3.148 ± 0.085	5.303ValGly: 5.303 ± 0.12	1.12ValHis: 1.12 ± 0.042	5.328ValIle: 5.328 ± 0.123	4.337ValLys: 4.337 ± 0.086	7.002ValLeu: 7.002 ± 0.131	2.199ValMet: 2.199 ± 0.073	2.682ValAsn: 2.682 ± 0.081	2.725ValPro: 2.725 ± 0.066	1.685ValGln: 1.685 ± 0.053	3.442ValArg: 3.442 ± 0.085	5.096ValSer: 5.096 ± 0.111	4.487ValThr: 4.487 ± 0.108	5.311ValVal: 5.311 ± 0.118	0.654ValTrp: 0.654 ± 0.038	2.61ValTyr: 2.61 ± 0.072	0.002ValXaa: 0.002 ± 0.002
Trp	0.685TrpAla: 0.685 ± 0.04	0.222TrpCys: 0.222 ± 0.022	0.569TrpAsp: 0.569 ± 0.042	0.476TrpGlu: 0.476 ± 0.028	0.428TrpPhe: 0.428 ± 0.029	0.7TrpGly: 0.7 ± 0.042	0.19TrpHis: 0.19 ± 0.02	0.474TrpIle: 0.474 ± 0.027	0.453TrpLys: 0.453 ± 0.035	1.007TrpLeu: 1.007 ± 0.046	0.284TrpMet: 0.284 ± 0.028	0.387TrpAsn: 0.387 ± 0.029	0.238TrpPro: 0.238 ± 0.022	0.316TrpGln: 0.316 ± 0.025	0.42TrpArg: 0.42 ± 0.031	0.56TrpSer: 0.56 ± 0.044	0.445TrpThr: 0.445 ± 0.034	0.581TrpVal: 0.581 ± 0.032	0.081TrpTrp: 0.081 ± 0.012	0.387TrpTyr: 0.387 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.058TyrAla: 3.058 ± 0.082	0.752TyrCys: 0.752 ± 0.045	2.6TyrAsp: 2.6 ± 0.078	2.192TyrGlu: 2.192 ± 0.069	1.616TyrPhe: 1.616 ± 0.063	2.771TyrGly: 2.771 ± 0.069	0.587TyrHis: 0.587 ± 0.04	2.55TyrIle: 2.55 ± 0.074	2.054TyrLys: 2.054 ± 0.065	3.189TyrLeu: 3.189 ± 0.096	1.097TyrMet: 1.097 ± 0.044	1.678TyrAsn: 1.678 ± 0.072	1.29TyrPro: 1.29 ± 0.044	0.752TyrGln: 0.752 ± 0.043	1.946TyrArg: 1.946 ± 0.061	2.176TyrSer: 2.176 ± 0.071	2.174TyrThr: 2.174 ± 0.089	2.186TyrVal: 2.186 ± 0.066	0.458TyrTrp: 0.458 ± 0.038	1.641TyrTyr: 1.641 ± 0.091	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.004XaaPro: 0.004 ± 0.003	0.0XaaGln: 0.0 ± 0.0	0.008XaaArg: 0.008 ± 0.004	0.002XaaSer: 0.002 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.002	0.036XaaXaa: 0.036 ± 0.01

Statistics based on 1723 proteins (521547 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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