Enterococcus phage EFDG1
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 192 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5K8N6|A0A0C5K8N6_9CAUD Uncharacterized protein OS=Enterococcus phage EFDG1 OX=1597976 PE=4 SV=1
MM1 pKa = 7.69 LSTAEE6 pKa = 3.92 YY7 pKa = 10.61 DD8 pKa = 3.57 SLRR11 pKa = 11.84 EE12 pKa = 3.76 YY13 pKa = 11.0 LVAVIHH19 pKa = 6.15 NEE21 pKa = 3.14 ISEE24 pKa = 4.35 YY25 pKa = 11.25 GGDD28 pKa = 3.83 TQSLDD33 pKa = 3.08 VWKK36 pKa = 10.86 EE37 pKa = 3.87 EE38 pKa = 3.92 VSKK41 pKa = 11.37 YY42 pKa = 10.21 ADD44 pKa = 3.69 NRR46 pKa = 11.84 IGWYY50 pKa = 9.12 LRR52 pKa = 11.84 CMNLADD58 pKa = 4.9 LLYY61 pKa = 10.3 VDD63 pKa = 5.36 TPASLADD70 pKa = 3.15 IYY72 pKa = 11.29 KK73 pKa = 10.4 DD74 pKa = 3.44 YY75 pKa = 11.28 KK76 pKa = 11.04 EE77 pKa = 4.29 EE78 pKa = 4.1 LNEE81 pKa = 3.72 ILRR84 pKa = 11.84 DD85 pKa = 3.86 YY86 pKa = 11.04 TEE88 pKa = 4.06 EE89 pKa = 4.39 LYY91 pKa = 11.03 AGATFDD97 pKa = 4.34 KK98 pKa = 10.85 EE99 pKa = 3.78 ADD101 pKa = 3.7 EE102 pKa = 4.36 RR103 pKa = 11.84 LGWMWEE109 pKa = 4.28 DD110 pKa = 3.68 YY111 pKa = 11.24 DD112 pKa = 4.28 PLILEE117 pKa = 4.27 AHH119 pKa = 5.65 NQGLVARR126 pKa = 11.84 LGLLIVAEE134 pKa = 4.07 QLATQLEE141 pKa = 4.61 QGTFTIPYY149 pKa = 9.94 DD150 pKa = 3.51 NN151 pKa = 4.39
Molecular weight: 17.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.813
IPC2_protein 4.062
IPC_protein 4.012
Toseland 3.834
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.872
Rodwell 3.846
Grimsley 3.745
Solomon 3.973
Lehninger 3.923
Nozaki 4.088
DTASelect 4.266
Thurlkill 3.859
EMBOSS 3.884
Sillero 4.126
Patrickios 1.138
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.006
Protein with the highest isoelectric point:
>tr|A0A0C5JZD1|A0A0C5JZD1_9CAUD PHB domain-containing protein OS=Enterococcus phage EFDG1 OX=1597976 PE=3 SV=1
MM1 pKa = 7.41 RR2 pKa = 11.84 NKK4 pKa = 10.49 YY5 pKa = 9.66 PGYY8 pKa = 10.09 CYY10 pKa = 10.19 RR11 pKa = 11.84 CGTFVEE17 pKa = 4.71 KK18 pKa = 10.92 GQGHH22 pKa = 5.88 FEE24 pKa = 4.19 RR25 pKa = 11.84 KK26 pKa = 8.35 NGYY29 pKa = 8.56 WRR31 pKa = 11.84 LQHH34 pKa = 6.85 ADD36 pKa = 3.29 CAIKK40 pKa = 10.81 YY41 pKa = 10.09 RR42 pKa = 11.84 GTNVQGNGTRR52 pKa = 11.84 PTKK55 pKa = 9.61 EE56 pKa = 3.49 RR57 pKa = 11.84 KK58 pKa = 9.13
Molecular weight: 6.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.27
IPC2_protein 9.516
IPC_protein 9.677
Toseland 9.97
ProMoST 9.838
Dawson 10.218
Bjellqvist 9.955
Wikipedia 10.423
Rodwell 10.54
Grimsley 10.306
Solomon 10.262
Lehninger 10.218
Nozaki 10.058
DTASelect 9.926
Thurlkill 10.058
EMBOSS 10.394
Sillero 10.16
Patrickios 10.087
IPC_peptide 10.262
IPC2_peptide 9.004
IPC2.peptide.svr19 8.332
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
192
0
192
43464
41
2199
226.4
25.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.136 ± 0.244
0.867 ± 0.093
6.35 ± 0.162
8.096 ± 0.305
3.792 ± 0.104
6.339 ± 0.213
1.622 ± 0.094
6.274 ± 0.143
7.675 ± 0.187
8.133 ± 0.174
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.586 ± 0.078
5.342 ± 0.147
3.221 ± 0.121
3.861 ± 0.149
4.392 ± 0.143
5.927 ± 0.157
6.569 ± 0.213
7.029 ± 0.175
1.114 ± 0.073
4.675 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here