Sea turtle tornovirus 1
Average proteome isoelectric point is 8.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C0J6N2|C0J6N2_9VIRU Uncharacterized protein OS=Sea turtle tornovirus 1 OX=575913 PE=4 SV=1
MM1 pKa = 7.27 AQNAGEE7 pKa = 4.68 DD8 pKa = 3.93 NPDD11 pKa = 3.2 TTNPGEE17 pKa = 4.27 GTSQSFGGGNHH28 pKa = 6.38 SGATNYY34 pKa = 10.8 LDD36 pKa = 5.07 LIKK39 pKa = 10.93 LQGEE43 pKa = 4.02 YY44 pKa = 10.72 SFYY47 pKa = 10.22 KK48 pKa = 9.48 RR49 pKa = 11.84 HH50 pKa = 5.61 YY51 pKa = 10.13 NRR53 pKa = 11.84 ALAIWKK59 pKa = 9.36 RR60 pKa = 11.84 EE61 pKa = 3.67 CRR63 pKa = 11.84 EE64 pKa = 3.79 SHH66 pKa = 7.42 DD67 pKa = 5.21 RR68 pKa = 11.84 ICTCKK73 pKa = 9.73 DD74 pKa = 2.42 WRR76 pKa = 11.84 YY77 pKa = 9.12 HH78 pKa = 5.81 FSRR81 pKa = 11.84 AAEE84 pKa = 4.01 RR85 pKa = 11.84 PGIEE89 pKa = 4.1 ALQHH93 pKa = 6.28 SSQQSSRR100 pKa = 11.84 DD101 pKa = 3.63 LYY103 pKa = 11.05 SDD105 pKa = 4.18 PGADD109 pKa = 4.65 FYY111 pKa = 11.64 QQVQSLPQRR120 pKa = 11.84 YY121 pKa = 8.67 KK122 pKa = 10.86 PEE124 pKa = 3.74 RR125 pKa = 11.84 TLQNLEE131 pKa = 3.95 RR132 pKa = 11.84 EE133 pKa = 4.49 YY134 pKa = 10.92 TRR136 pKa = 11.84 VLARR140 pKa = 11.84 YY141 pKa = 8.22 KK142 pKa = 10.49 KK143 pKa = 10.03 LRR145 pKa = 11.84 KK146 pKa = 9.34 KK147 pKa = 10.07 QRR149 pKa = 11.84 QEE151 pKa = 3.23 KK152 pKa = 10.13 ARR154 pKa = 11.84 ARR156 pKa = 11.84 VQFTQKK162 pKa = 9.71 PGKK165 pKa = 9.37 RR166 pKa = 11.84 PKK168 pKa = 9.86 IKK170 pKa = 10.16 KK171 pKa = 8.13 EE172 pKa = 3.84 KK173 pKa = 9.84 RR174 pKa = 11.84 WPIDD178 pKa = 3.59 DD179 pKa = 5.42 DD180 pKa = 3.83 STEE183 pKa = 4.1 GDD185 pKa = 3.55 TTGEE189 pKa = 4.1 EE190 pKa = 4.4 DD191 pKa = 4.02 TDD193 pKa = 4.02 GTDD196 pKa = 4.33 GDD198 pKa = 4.08 ITGDD202 pKa = 4.1 DD203 pKa = 3.88 DD204 pKa = 4.02 TGGPTGDD211 pKa = 3.73 GGSFLDD217 pKa = 4.13 PSSVRR222 pKa = 11.84 AASRR226 pKa = 11.84 FLL228 pKa = 3.76
Molecular weight: 25.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.109
IPC2_protein 7.468
IPC_protein 7.571
Toseland 7.38
ProMoST 7.951
Dawson 8.2
Bjellqvist 8.331
Wikipedia 8.156
Rodwell 8.2
Grimsley 7.38
Solomon 8.39
Lehninger 8.39
Nozaki 8.463
DTASelect 8.229
Thurlkill 8.273
EMBOSS 8.434
Sillero 8.551
Patrickios 4.571
IPC_peptide 8.375
IPC2_peptide 7.366
IPC2.peptide.svr19 7.591
Protein with the highest isoelectric point:
>tr|C0J6N3|C0J6N3_9VIRU Uncharacterized protein OS=Sea turtle tornovirus 1 OX=575913 PE=4 SV=1
MM1 pKa = 7.61 LEE3 pKa = 4.53 KK4 pKa = 10.17 IILTLLILVRR14 pKa = 11.84 EE15 pKa = 4.51 LLRR18 pKa = 11.84 ALAGGIILVLLIIWILSSYY37 pKa = 9.24 KK38 pKa = 10.87 ANIAFTRR45 pKa = 11.84 DD46 pKa = 3.51 TITVHH51 pKa = 6.31 LPSGRR56 pKa = 11.84 EE57 pKa = 3.88 SAEE60 pKa = 3.76 KK61 pKa = 10.85 AMTEE65 pKa = 4.11 SVPAKK70 pKa = 9.79 TGATTSPEE78 pKa = 3.93 QLKK81 pKa = 10.91 DD82 pKa = 3.53 LALKK86 pKa = 10.28 LCSIHH91 pKa = 6.91 LSNPPAISTVTPEE104 pKa = 3.7 RR105 pKa = 11.84 TSTNKK110 pKa = 10.25 YY111 pKa = 9.88 KK112 pKa = 10.84 VYY114 pKa = 10.86 LSATSQKK121 pKa = 10.4 GPSKK125 pKa = 9.08 TLSVSTRR132 pKa = 11.84 GSWHH136 pKa = 4.5 VTRR139 pKa = 11.84 SSGRR143 pKa = 11.84 SSAKK147 pKa = 9.66 RR148 pKa = 11.84 RR149 pKa = 11.84 LEE151 pKa = 3.78 PEE153 pKa = 3.96 FNSPRR158 pKa = 11.84 SLEE161 pKa = 3.71 RR162 pKa = 11.84 DD163 pKa = 3.22 LRR165 pKa = 11.84 SKK167 pKa = 10.54 KK168 pKa = 10.58 KK169 pKa = 10.32 KK170 pKa = 10.36 DD171 pKa = 3.41 GLL173 pKa = 3.84
Molecular weight: 19.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.198
IPC2_protein 9.531
IPC_protein 9.648
Toseland 10.599
ProMoST 10.101
Dawson 10.687
Bjellqvist 10.277
Wikipedia 10.804
Rodwell 11.184
Grimsley 10.701
Solomon 10.73
Lehninger 10.716
Nozaki 10.57
DTASelect 10.277
Thurlkill 10.57
EMBOSS 10.979
Sillero 10.599
Patrickios 10.921
IPC_peptide 10.745
IPC2_peptide 8.77
IPC2.peptide.svr19 8.661
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
711
173
310
237.0
27.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.188 ± 0.302
0.703 ± 0.259
4.501 ± 1.946
4.079 ± 1.539
3.657 ± 1.163
6.892 ± 1.096
3.094 ± 0.811
4.219 ± 1.038
6.048 ± 1.318
8.158 ± 2.186
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.547 ± 0.58
4.501 ± 1.298
5.063 ± 0.1
4.923 ± 1.442
9.423 ± 0.757
8.861 ± 1.692
8.298 ± 0.555
3.516 ± 0.743
2.672 ± 1.03
3.657 ± 0.733
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here